ACETYLTRANSFERASE (PLATELET-ACTIVATING FACTOR SYNTHESIS) ACTIVITY OF NEUTROPHILS IN INFLAMMATORY DISORDERS: EFFECTS OF CYTOKINES INCLUDING TUMOR NECROSIS FACTOR.
| Project/Area Number |
63571105
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | YAMANASHI MEDICAL COLLEGE |
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Principal Investigator |
OZAKI Yukio DEPARTMENT OF CLINICAL AND LABORATORY MEDICINE, ASSISTANT PROFESSOR, 医学部, 講師 (30134539)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1988: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | NEUTROPHILS / PLATELET-ACTIVATING FACTOR / CYTOKINE / TUMOR NECROSIS FACTOR / 血小板活性化因子合成能 / tumor necrosis factor / interleukin1 |
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| Research Abstract |
Of the five cytokines tested (tumor necrosis factor, interleukin 1, colony-stimulating factor, interferon alpha, beta, and gamma), only tumor necrosis factor (TNF) showed a potent stimulatory effect on the acetyltransferase activity of human neutrophils, which is a key enzyme for the production of platelet-activating factor. This potentiating effect of TNF was time- and dose-dependent and could be effectively blocked by antiserum against TNF. None of Intracellular Ca^<++> elevation, protein kinase C activation, and arachidonic acid metabolites appeared to be involved in the TNF-induced potentiation of the acetyltransferase activity in neutrophils, and the mechanism of TNF potentiation remains to be clarified.
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Report
(3 results)
Research Products
(7 results)
