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Molecular mechanisms of protein sorting between the mitochondrial outer and inner membranes

Research Project

Project/Area Number 63580151
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 代謝生物化学
Research InstitutionNagoya University

Principal Investigator

HASE Toshiharu  Nagoya University, Department of Agricultural Chemistry, Associate Professor, 農学部, 助教授 (00127276)

Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsMitochondria / Membrane proteins / Intracellular transport of proteins / Cytochrome c_1 / チトクロムc_1
Research Abstract

Mitochondrial proteins synthesized in the cytoplasm are transported and localized to a specific compartment of the mitochondrion. The intramitochondrial sorting between the outer and inner membranes was investigated using a 70 kDa outer membrane protein and cytochrome c_1 as representative proteins in yeast cells. The presequence of cytochrome c_1, which functions as a signal to the inner membrane was replaced by various lengths of the N-terminal region of the 70 kDa protein. The fused protein with the N-terminal 61 residues(61mC_1) was localized to the outer membrane and those with less than 29 residues to the inner membrane. The latter fusion proteins were shown to be assembled into succinate-eytochrome c reductase without proteolytic removal of the fused region and restore the respiration of a cytochrome c_1 deficient mutant yeast(MH6-16). These date indicate that targeting and anchoring signals of the outer membrane protein localize the inner membrane protein to the outer membrane and that the target sequence alone directs it to the inner membrane.
The cytochrome c_1 deficient mutant yeast, MH6-16 transformed with a plasmid encoding 61mC_1 was not able to grow on a non- fermentable medium(glycerol^-), due to location of this protein to the outer membrane. A selection for mutations which restored growth on glycerol was applied to the transformants and yield a class of invitation in the host genome. We are now characterizing this mutation as a mitochondrial protein sorting mutant and a DNA fragment involved in the restoration are currently isolated.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 長谷俊治: "真核細胞における蛋白質の細胞内輸送の分子機構" 生物物理. 28. 1-6 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M.Nakai: "Precise determination of the mitochondrial import signal contained in a 70 kDa protein of yeast mitochondrial outer membrane" Journal of Biochemistry. 105. 513-519 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M.Nakai: "Protein sorting between the outer and inner mitochondrial membrane:submitochondrial localization of cytochrome c1 whose presequence is replaced by the amino-terminal region of a 70 kDa outer membrane" Journal of Biochemistry. 106. 181-187 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M.Nakai: "Isolation of a yeast gene involved in the protein sorting between the outer and iiner mitochondrial membranes"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M. Nakai et. al.: "Precise determination of the mitochondrial import signal contained in a 70 kDa protein of yeast mitochondrial outer membrane" J. Biochemistry 105, 513-519 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M. Nakai et. al.: "Protein sorting between the outer and inner mitochondrial membranes: submitochondrial localization of cytchome c_1 whose presequence is replaced by the amino-terminal region of a 70 kDa outer membrane" J. Biochemistry 106, 181-187 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M.Nakai: "Precise determination of the mitochondrial import signal contained in a 70kDa protein of yeast mitochondrial outer membane" J.Biochemistry. 105. 513-519 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Nakai: "Protein sorting between the outer and inner mitochondrial membrane:submitochondrial localization of cytochrome c_1 whose presequence is replaced by the amino-terminal region of a 70 kDa outer membrane" J.Biochemistry. 106. 181-187 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 長谷俊治: 生物物理. 28. 1-6 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Nakai: Journal of Biochemistry. 105. (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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