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DNA Synthesis on Porous Glass Supports

Research Project

Project/Area Number 63850186
Research Category

Grant-in-Aid for Developmental Scientific Research (B).

Allocation TypeSingle-year Grants
Research Field 高分子合成
Research InstitutionMiyazaki University

Principal Investigator

IMADA Kiyohisa  Miyazaki University, Faculty of Engineering, Department of Industrial Chemistry Professor, 工学部, 教授 (00037748)

Co-Investigator(Kenkyū-buntansha) HAYSHI Satio  Miyazaki University, Faculty of Engineering, Department of Industrial Chemistry, 工学部, 助教授 (90148916)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1988: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsPorous Glass / DNA Synthesis / Chemical Modification of Glass Surface / ガラス表面の化学修飾 / 多孔質ガラス / DNA合成 / ガラス多孔体 / ガラス担体
Research Abstract

The surface structure of porous glasses, which would be used as supports in DNA syntheses, was investigated. Silanol groups on the surface were estimated to be 7-9mumol・m^<-2>. The value was compared with the crystal structure of cristobalite, as a model structure of SiO_2 glass. Amino-alkyl functionalities obtained by silane coupling agents was estimated to be 2-3mumol・m^<-2>. Control of the surface densities of amino-alkyl functionalities by adding alkyl silane, such as methyl trimethoxysilane, was useful to avoid the stereochemical interactions hindering the growth of the DNA chains. Amino-alkylation of the glass surface of 5mumol・m^<-2> was found as the optimum density. Pore diameters and chemical compositions of the skeletal glass were optimized.
A clone of carboxymethyl cellulose degrading enzyme obtained from a wild strain of Bacillus was established using E. coli K12, HB101, as a host, and pBR322 plasmid as a vector. This could be used in further studies to develop wider range of uses of the porous glass substrata, in many aspects of protein engineering, such as DNA and peptide syntheses, immobilized enzymes, affinity chromatography, etc.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] 今田 清久,坂上 勝伺,川端 裕人,堤 信夫,中島 忠夫,信原 一敬: ""多孔質ガラスの表面状態とその化学修飾"" 日本化学会誌. 407-414 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.Hayashi,K.Imada et al.: ""Production of a fructosyl transferring enzyme by Aureobasidium sp.ATCC 20524"" J.Industrial Microbiology. 5. 395-400 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.Hayashi,K.Imada et al.: "Obseervation of the Chemical Structure of Fructooligosaccharide Produced by an Enzyme from Aureobasidium" Current Microbiology. 19. 175-177 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.OKUYAMA,K.Imada et al.: "Molecular Structure of AibーContaining Oligopeptides Bocー(Leu_3ーAib)_2ーOBzlー and Boeー(Leu_3ーAib)_3ーOBzl" Polymer Journal. 20. 707-710 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.Hayashi,M.Nonoguchi,Y.Takasaki,H.Ueno and K.Imada: "″Purification and Properties of βーFuructofuranosidase from Aureobasidium sp. ATCC 20524"" J.Industrial Microbiology. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.Hayashi,M.Nonoguchi,Y.Takasaki,H.Ueno and K.Imada: ""Nutritional Status of Aureobasidium sp.ATCC 20524 for the production of βーFuructofuranosidase"" World J.Microbiology and Biotechnology. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 今田 清久: "泉フォラム、Int.Workshop on Suprahybridization between Biocomponents and Artificial Materials 講演予稿集 (Possibilities of Glass Surface as the Support for Biological and Medical Uses./Porous Glass Support for DNA Synthesis.)" (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 今田 清久: "SPG研究論文集 (多孔質ガラスの表面状態と細孔内表面の化学修飾,多孔質ガラス支持体による核酸の合成)" SPG研究会, (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. IMADA, K. SAKAUE, H. KAWABATA, N. TSUTSUMI, T. NAKAJIMA, K. NOBUHARA: "Studies on the Surface of Porous Glass and Chemical Modification thereof" J. Chem. Soc. Japan. 1990(4). 407-414 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Hayashi, M. Nonoguchi, H. Ueno, and K. Imada: "Production of a fructosyltransferring enzyme by Aureobasidium sp. ATCC 20524" J. Industrial Microbiology. 5. 395-400 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Hayashi, K. Imada, Y. Kushima, H. Ueno: "Observation of the Chemical Structure of Fructooligosaccharide Produced by an Enzyme from Aureobasidium sp. ATCC20524" Current Microbiology. 177. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hayashi, M. Nonoguchi, Y. Takasaki, H. Ueno, and K. Imada: "Purification and Properties of beta-Fuructofuranosidase from Aureobasidium sp. ATCC 20524" J. Industrial Microbiology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Hayashi, M. Nonoguchi, Y. Takasaki, H. Ueno, and K. Imada: "Nutritional Status of Aureobasidium sp. ATCC 20524 for the production of beta-Fuructofuranosidase" World J. Microbiology and Biotechnology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. IMADA, K. SHIMA, K. KUSANO, T. NAKASHIMA: "Characteristics of the Internal Surface of Porous Glass and Chemical Modification thereof" Reports on Researches and Developments of Sirasu Porous Glass. 100-107 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. IMADA, K. SHIMA, K. KUSANO, T. NAKASHIMA, N. TSUTSUMI: "DNA Synthesis on Porous Glass Support" Reports on Researches and Developments of Sirasu Porous Glass. 108-116 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. IMADA, M. URABE: Shoka Bou. Polymer Chemistry, (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 今田 清久,坂上 勝伺,川端 裕人,堤 信夫,中島 忠夫,信原 一敬: "“多孔質ガラスの表面状態とその化学修飾"" 日本化学会誌. 407-414 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Hayashi,K.Imada et al.: "“Production of a fructosyl transferring enzyme by Aureobasidium sp.ATCC 20524"" J.Industrial Microbiology. 5. 395-400 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Hayashi,K.Imada et al.: "Obseervation of the Chemical Structure of Fructooligosaccharide Produced by an Enzyme from Aureobasidium" Current Microbiology. 19. 175-177 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.OKUYAMA,K.Imada et al.: "Molecular Structure of AibーContaining Oligopeptides Bocー(Leu_3ーAib)_2ーOBzl andBocー(Leu_3ーAib)_3ーOBzl" Polymer Journal. 20. 707-710 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Hayashi,M.Nonoguchi,Y.Takasaki,H.Ueno and K.Imada: "“Purification and Properties of βーFuructofuranosidase from Aureobasidium sp.ATCC 20524"" J.Industrial Microbiology. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Hayashi,M.Nonoguchi,Y.Takasaki,H.Ueno and K.Imada: "“Nutritional Status of Aureobasidium sp.ATCC 20524 for the production of βーFuructofuranosidase"" World J.Microbiology and Biotechnology. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 今田 清久: "泉フォラム、Int.Workshop on Suprahybridization between Biocomponents and Artificial Materials 講演予稿集" Possibilities of Glass Surface as the Support for Biological and Medical Uses.Porous Glass Support for DNA Synthesis.2-2 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 今田 清久: "SPG研究論文集・SPG研究会" 多孔質ガラスの表面状態と細孔内表面の化学修飾 多孔質ガラス支持体による核酸の合成. 8-9 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 今田清久,坂上勝伺,川端裕人,堤信夫,中島忠夫,信原一敬: "多孔質ガラスの表面状態とその化学修飾" 日本化学会誌. 1990. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 今田清久,志摩健介,草野一仁,中島忠夫: "多孔質ガラスの表面状態と細孔内表面の化学修飾" SPG研究論文集(SPG応用技術研究会). 100-106 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 今田清久,志摩健介,草野一仁,中島忠夫,堤信夫: "多孔質ガラス支持体による核酸の合成" SPG研究論文集(SPG応用技術研究会). 108-116 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.HAYASHI: "Ovservation of the Chemical Strueture of Fructooligo Saccharide Produced by and Enzyme from Aureofasidlum sp.ATCC 20524." Current Microbiology. 19. 175-177 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.HAYASHI: "Production of a fructosyl transferring enzyme by Aureo basidium sp.ATCC 20524" Journal of Industrial Microbiology.

    • Related Report
      1989 Annual Research Report
  • [Publications] 今田清久,林幸男,中島忠夫,村上昭弘: 日本化学会誌.

    • Related Report
      1988 Annual Research Report
  • [Publications] 今田清久,坂上勝伺,堤信夫: 第11回SPG応用技術研究会・研究発表要旨集. 13-16 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 今田清久,中島忠夫: 第11回機能材料と利用に関するフォーラム講演予稿. 8-11 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 今田清久: ガラス表面研究会討論会資料. 22-25 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Hayashi;et al.: Current Microbiology.

    • Related Report
      1988 Annual Research Report
  • [Publications] 今田清久,占部正義: "高分子化学" 裳華房, 1-167 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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