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Identification of plant variety on the base of glycoside components such as saponin

Research Project

Project/Area Number 63860001
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionTohoku University

Principal Investigator

HINATA Kouichi  Fac. Agriculture, Tohoku Univ., Professor, 農学部, 教授 (00005589)

Co-Investigator(Kenkyū-buntansha) IWABUCHI Setsuko  Fac. Agriculture, Tohoku Univ., Res. Fellow, 農学部, 教務員 (50089794)
ASANO Mitsuo  Fac. Agriculture, Tohoku Univ., Assistant, 農学部, 教務員 (40089793)
OKUBO Kazuyoshi  Fac. Agriculture, Tohoku Univ., Professor, 農学部, 教授 (00005612)
MOTOMURA Yosie  Fac. Agriculture, Tohoku Univ., Assistant, 農学部, 助手 (50005609)
HARADA Hisaya  Nat. Inst. Agrobiol. Res., Head, 室長
Project Period (FY) 1987 – 1990
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥18,200,000)
Fiscal Year 1990: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1989: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1988: ¥10,800,000 (Direct Cost: ¥10,800,000)
KeywordsSoybean / Glycoside / Saponin / Soyasapogenol / Group A saponin / Group B saponin / Glycine max / Glycine soja / ブド- / 品種 / 大豆 / ビスデスモサイドトリテルペンサポニン / ケモタキソノミー
Research Abstract

(1) In this study, identifications of a plant variety was attemted based on the analysis of glycoside compomemts in some part of the plant such as soybean, rape, rice and grape.
(2) In the soybean, the composition analysis of group A saponin, which are located only in the seed hypocotyl, has provided data on not only the classification of the variety but also a prospect of finding an original variety of it.
(3) It was the first findings that group A saponin had a sugar, in which hydroxyl groups are fully acetylated, at the terminal position of the sugar chain attached at the C-22 position of soyasapogenol A, and that Aa-type which has a xylose residure at that position and Ab-type which has a glucose residure were controlled by a codominant allele at a single locus.
(4) Group A saponins are detected in the subgenus Soja, and Aa-type would be a marker for the wild soybean, G. soja, and Ab-type would be a marker for the cultivated soybean, G. max. This finding will play an important role to collect the germplasm which competes worldwide.
(5) As compared with group A saponin, group B saponin is a marker for the Leguminosae and distributes widely to it, and it was supposed to be a starting material in vivo for synthesizing glycchirrichin which is used as a medicine.
(6) Extraction, TLC and HPLC conditions for glucoside component analysis have been established using representative variety seed of rice, soybean, rape as well as grape and leaves of peach and grape, and typical patterns of TLC and HPLC analyses have also been obtained. This led a finding of unique components to the plant and unknown components.
(7) In the genus Vitis, there was a clear difference in each classification of Muscadinia, North America, East Asia and Euracia.
(8) These results will contribute to the improvement and identiication of the variety as well as the classification of plants.

Report

(4 results)
  • 1991 Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Makoto SHIMOYAMADA et al.: "Distribution of saponin constituents in soybean plant of some varieties" Agric.Biol.Chem.54. 77-81 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] ShiGemitsu KUDOU et al.: "Enzymatic hydrolysis of soybean saponin by Aspergillus oryzae KO-2" Agric.Biol.Chem.54. 1341-1342 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masakazu SHIRAIWA et al.: "Inheritance of "group A sapoin in soybean seed" Agric.Biol.Chem.54. 1347-1352 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 元村 佳恵等: "ブド-属植物の葉に含まれるサポニン様物質による品種群の識別" 園芸学雑誌. 59. 72-73 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Makoto SHIMOYAMADA et al.: "Antifungal activity of saponin fraction Obtained from Asparagus of ficinal L.and its principle" Agric.Biol.Chem.54. 2553-2557 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shigemitsu KUDOU et al.: "Screening for microorganisms producing soybean hydrolase" Agric.Biol.Chem.54. 3035-3037 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shigemitsu KUDOU et al.: "Purification and some properties of soybean saponin hydrolase from Aspergillus orezae KO-2" Agric.Biol.Chem.55. 31-36 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masakazu SHIRAIWA et al.: "Composition and structure of “group A saponin" in soybean seed" Agric.Biol.Chem.55. 315-322 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masamitsu KUDOU et al.: "Malonyl isoflavone glycosides in soybean seeds" Agric.Biol.Chem.55. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ryzard AMAROWICZ et al.: "The content of crude glycosides and glucosinolates in the Japanese rape seed varieties" Die Nahrung-Food. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Roger G.FENWICK et al.: "Saponin;Toxic substance in crop plant" Royal Society of Chemistry,UK, (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Makoto SHIMOYAMADA et al.: "Distribution of saponin constituents in soybean plant of some varieties" Agric.Biol.Chem.54. 77-81 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shigemitsu KUDOU et at.: "Enzymatic hydrolysis of soybean s<ap>___ーon<in by A.>___ーoryzae KOー2" Agric.Biol.Chem.54. 1341-1342 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masakazu SHIRAIWA et al.: "Inheritance of “group A saponin in soybean seed" Agric.Biol.Chem. 54. 1347-1352 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 元村 佳恵等: "ブド-属植物の葉に含まれるまれる配糖体成分による品種群の識別" 園芸学雑誌. 59. 72-73 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Makoto Shimoyamada et al.: "Antifugal activity of saponin fraction obtained from Asparagus of ficinal L.and its principle" Agric.Biol.Chem.54. 2553-2557 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shigemitsu KUDOU et al.: "Screening for microorganisms producing soypean saponin hydrolase" Agric.Biol.Chem.54. 3035-3037 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shigemitsu KUDOU et al.: "Purification and some properties of soybean saponin hydrase from A<spergillus orezae KOー2>___ー" Agric.Biol.Chem.55. 31-36 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masamitsu SHIRAIWA et al.: "Composition and structure of “group A saponin" in soybean seed" Agric.Biol.Chem.55. 315-322 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masamitsu SHIRAIWA et al.: "Composition and content of saponin in soybean seed according to variety,annual cultivation and harvest time" Agric.Biol.Chem.55. 323-331 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Makoto Shimoyamada et al.: "Variation in saponin levels and contents in germination of soybean seed and effect of light irradiation" Agric.Biol.Chem.55. 577-579 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shigemitsu KUDOU et al.: "A new isoflavon in soybean seeds (Glycine max L.Merrill);glycitein 7ーOーβーDー(6“ーOーacetyl)ーglucopyranoside" Agric.Biol.Chem.55. 859-860 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masamitsu SHIRAIWA et al.: "Composition and structure of “group B saponin" in soybean seed" Agric.Biol.Chem.55. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ryzard AMAROWICZ et al.: "Application of reverse phase liquid chromatography in the analysis of saponin in faba bean" Die NahrungーFood(German). No.2. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Makoto Shimoyamada et al.: "Saponin composition in developing soybean seed (Glycine max L.Merril,cv.Mikuriyaao)" Agric.Biol.Chem.55. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masamitsu SHIRAIWA et al.: "Soybean saponin:Structure and physiological properties,especially antiviral activity of HIV in vitro" Proc.International Coference Soybean Processing and Utilization. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 下山田 真等: "大豆サポニンの共優性遺伝大豆の起源を探れるかー" 化学と生物. 29. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Roger G.FENWICK et al.: "Saponin;Toxic substance in crop plant" Royal Society of Chemistory,UK, (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hideki NAKASHIMA et al.: "Inhibitory effect of glycisides like saponin from soybean on the infectivity of HIV in vitoro" AIDS. 3. 655-658 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 大久保一喜等: "醤油粕の有効利用に関する研究(第1報)醤油粕たんぱく質の化学的性質について" 日本醸造協会誌. 84. 869-875 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Makoto SHIMOYAMADA et al.: "Distribution of saponin constituents in soybean plant of some varieties" Agric.Biol.Chem.54. 77-81 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Masamitsu SHIRAIWA et al.: "Inheritance of″group A saponin″in soybean seed" Agric.Biol.Chem.54. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shigemitsu KIDO et al.: "Enzymatic hydrolysis of soybean saponin by Aspergillus oryzae KO-2" Agric.Biol.Chem.54. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Masamitsu SHIRAIWA et al.: "Composition and structure of″group A saponin″in soybean seed" Agric.Biol.Chem.54. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 大久保一良: 日本食品工業学会誌. 35. 866-874 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Isao KITAGAWA;et al.: Chem.Pharm.Bull.36. 2819-2828 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2019-02-15  

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