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Studies on the Production and Improvement of Flavors of Fishes and Shells in Microalgae

Research Project

Project/Area Number 63860027
Research Category

Grant-in-Aid for Developmental Scientific Research (B).

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionKyoto University

Principal Investigator

ISHIDA Yuzaburo  Microbiology, Dept. of Fisheries, Professor, 農学部, 教授 (20026488)

Co-Investigator(Kenkyū-buntansha) KAWAI Tetsuo  Lab. of Flavor Substances, Shionokoryo Kaisha Ltd., Head, 基礎研究部, 主任
KANISAWA Thuneyoshi  General Institute, Takasago Koryo Kogyo. Manager, 総合研究所, 部長
NAKAJIMA Kenji  Dept. of Nutrition, Koshienn Univ., Professor, 栄養学部, 教授 (60131512)
UCHIDA Aritsune  Microbiology, Dept. of Fisheries, Assoc. Professor, 農学部, 助教授 (50027190)
SAKAGUCHI Morihiko  Chemistry, Dept. of Fisheries, Professor, 農学部, 教授 (00027187)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1988: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsFlavor / Microalgae / Crypthecodinium cohnii / Dimethylsulphide / Dimethyl-beta-Propiothetine / Dinoflagellate / Sulphor Cycle / Feeding Attractant / 09 Volatile Component / ジチメルーβープロピオテチン / メチオニン脱炭酸酵素 / 含硫香気成分 / 摂餌誘因 / ジメチル・β・プロピオテチン / 抗胃潰瘍性 / 揮発性イオウ化合物 / 揮発性含硫有機化合物 / 成長促進物質
Research Abstract

In this study, we concentrated to make clear the biosynthesis and role of volatile sulphur compound, especially dimethylsulphide, in microalgae and macroalgae. The major source of dimethylsulphide in the aquatic environment appears to be the enzymatic cleavage or alkaline degradation of dimethyl-beta-propio thetin into dimethylsulphide and acrylate. The dimethylsulphide producers are chlorophyta (such as Ulva and Enteromorpha), rhodophyta (such as Polysiphonia), haptopyta (such as Emiliania and hymenomonas) and dinophyta (such as Gymnodinium, Gyrodinium, and Crypthecodinium). This dimethylsulphide fills the role originally assigned to H2S ; that of transferring sulphur from the seas through the air to land surface, and is very likely the compound most responsible for the characteristic "odor of the sea" or "flavour of sea weeds".
We established the growth condition of Crypthecodinium cohnii (dinoflagellate) to get high cell yield (12g wet weight/L).
From the relationship between the stru … More ctures of methionine, dimethyl-beta-propiothetin and dimethyl-sulphide, our recent study make clear that, in C. Cohnii, the methyl group and the sulphur of dimethyl-beta-propiothetine are derived from the methyl group and the sulphur of methionine and that the carboxyl group of methionine is not incorporated. We showed the crude extracts of C. Cohnii had methionine decarboxylase activity and did not have methionine deaminase activity. The observation are consistent with the hypothesis that methionine is converted to 3-methylthiopropylamine by decarboxylation and that 3-methylthiopropylamine is converted to dimethyl-beta-propiothetin by deamination, oxidation and methylation, and that the carboxyl group of dimethyl-beta-propiothetine may arise from the 2-carbon of methionine.
We also found that the precursor of dimethylsulphide, dimethyl-beta-propiothetin, greatly accelerates the biting behaviors of goldfish, carp, crucian carp and tilapia. Dimethyl-beta-propiothetin proved not only to be an effective stimulant for the striking behavior of fishes but also to be able to promote fish growth. A crude extract of Crypthecodinium cohnii and its main component, dimethyl-beta-propiothetin, significantly and dose dependently protected the gastric mucosa against HCI-ethanolーinduced lesions in rats. Since indomethacin pretraetment reduced the protective effect to some extent, endogenous prostaglandins might be partly involved in the mechanism of action. Both the crude extract and dimethyl-beta-propiothetin significantly increased gastric secretion.
The volatile components of Crypthecodinium cohnii were investigated to find its pleasant flavour compounds that could be associated with some edible seashore products. Heating the fresh wet alga with glass beads gave 66 volatile components. The first intact distillate greatly contained dimethylsulphide nearly 99%. Other components included some strong sweet-odorus compounds ; 2ーhydroxy-3-methy1-2-cyclopenten-l-one (cyclotene), 2, 5-dimethyl-4-hydroxy-3 (2H) -furanone (furaneol), and 3-hydroxy-2-methyl-4H-pyran-4-one (maltol). Less

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] K.Nakajina,A.Uchida,and Y.Ishida: "Effect of supplemental dietary feeding attractant,dimethyーβーpropiothetin,on growth of gold fish." Nippon Suisan Gakkaishi. 55. 1291 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Kawai,Y.Ishida,H.Kakeuchi,N.Ikeda,and T.Tsuneya: "Volatile compounds of boiled and roasted shorーnecked clam Tapes philippinarum." Nippon Suisan Gakkaishi. 56. 795-802 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Nakajima,A.Uchida,and Y.Ishida: "Effect of a feeding attractant,dimethylーーpropiothetin,on growth of marine fishe." Nippon Suisan Gakkaishi. 56. 1151-1154 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y.Ishida,Y.Ogihara,and S.Okabe: "Effects of a crude extract of a marine dinoflagellate,containing dimethylーβーpropiothetin,on HClーethanolーinduced gastric lesions and gastric secretion in rats." Japan J.Pharmacol.54. 333-338 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Kawai,Y.Ishida,H.Kakeuchi,N.Ikeda,T.Higashida and S.Nakamura: "Flavor componets of dried squid." J.Agric.Food Chem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H.Komi,T.Kawai,T.Fujimura,A.Uchida,and Y.Ishida: "Comparison of volatile components between fresh and heated unicellular,alga,Crypthecodinium cohnii." J.Agric.Food Chem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 内田 有恆: "海洋科学・海洋微生物・研究の現状と展望 海洋におけるイオウサイクルと微生物" 海洋出版株式会社, 124 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 石田 祐三郎: "水産学シリ-ズ77 魚介類の生息環境と着臭(III.生物産生物質による着臭,7.微生物由来の着臭)" 恒星社厚生閣, 114 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] ARITSUNE UCHIDA: "Sulphur cycle and microbes in the ocean" Ocean Science. 120. 74-79 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] KENJI NAKAJIME, ARITSUNE UCHIDA AND YUZABURO ISHIDA: "Effects of heterocyclic sulfur-containing compounds on the striking response of goldfish." Memoirs of Koshien University. 16. 7-10 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] YUZABURISHIDA: "Off-Flavor originated from microbes." Off-Flavor and the Growing Environment of Fishes and Shellfishes. 70-80 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] MICHIYO MURATA AND MORIHIKO SAKAGUCHI: "Rapid accumulation of inosine 5'- monophosphate during heating the muscle of very fresh yellowtail." Nippon Suisan Gakkaishi. 55. 823-828 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] KENJI NAKAJIMA, ARITSUNE UCHIDA AND YUZABURO ISHIDA: "Effect of supplemental dietary feeding attractant, dimethyl-beta-rpopiothetin, on growth of goldfish." Nippon Suisan Gakkaishi. 55. 1291 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] TETSUO KAWAI AND YUZABURO ISHIDA: "Comparison of volatile components of dried squid to reaction products formed from the mixtures of hydrogen sulfide, ammonia, and aldehydes" J. Agric. Food Chem.37. 1026-1031 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] MORIHIKO SAKAGUCHI: "Mechanisms of developing flavor of fishr and shellfish" Bulletin of the research institute for food science, Kyoto University. No. 52. 30-33 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] TETSUO KAWAI, YUZABURO ISHIDA, HIROFUMI KAKIUCHI, NOBUO IKEDA AND TOMOYUKI TSUNEYA: "Volatile compounds of boiled and roasted short-necked clam Tapes philippinarum" Nippon Suisan Gakkaishi. 56. 795-802 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] MICHIYO MURATA, MORIHIKO SAKAGUCHI, TETSUJI SHIMIZU AND HAJIME EGUCHI: "Changes in flavor profile in boiled muscle extracts of yellowtall Seriola quinqueradiata Stored in ice" Nippon Suisan Gakkaishi. 56. 697 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] TETSUO KAWAI, YUZABURO ISHIDA AND TOSHIHISA HIGASHIDA: "Elimination of unpleasant odors of putrefied seafoods" Nippon Suisan Gakkaishi. 56. 1013 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] KENJI NAKAJIMA, ARITSUNE UCHIDA AND YUZABURO ISHIDA: "Effect of a feeding attractant dimethyl-beta-propiothetine, on growth of marine fish" Nippon Suisan Gakkaishi. 56. 1151-1154 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] YUZABURO ISHIDA, YOSHIYASU OGIHARA AND SUSUMU OKABE: "Effects of a crude extract of a marine dinoflagellate, containing dimethyl-beta-propiothetine, on HCl-ETHANOL-INDUCED Gastric lesions and gastric secretion in rats" Japan J. Pharmacol.54. 333-338 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] ARITSUNE UCHIDA: "Distribution of dimethylsulphide in the tropical and subtropical pacific ocean" Preliminary report of the Hakuho Maru Cruise KH-8-1. 66-77 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] TETSUO KAWAI, YUZABURO ISHIDA, HIROFUMI KAKEUCHI, NOBUO IKEDA, TOSHIHISA HIGASHIDA AND SHIGEO NAKAMURA: "Flavor components of dried squid." J. AGRIC. FOOD CHEM.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] HIROYUKI KOMI, TETSUO KAWAI, TAICHIRO FUJIMURA, ARITSUNE UCHIDA AND YUZABURO ISHIDA: "Comparison of volatile components between fresh and heated unicellular marine alga" Crypthecodinium cohnii.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Kawai,Y.Ishida,H.Kakiuchi,N.Ikeda and T,Tsuneya: "Volatile compounds of boiled and roasted shorーnecked clam <Tapes>___ー <Philippinarum>___ー." Nippon Suisan Gakkaishi. 56. 795-802 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Murata,M.Sakaguchi T.Shimizu and H.Eguchi: "Changes in flavor profile in boiled muscle extracts of yellowtail <Seriola>___ー <quinqueradiata>___ー stored in ice." Nippon Suisan Gakkaishi. 56. 697 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Kawai,Y.Ishida,and T.Higashida: "Elimination ofunpleasant odors of putrefied seafoods." Nippon Suisan Gakkaishi. 56. 1013 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Nakajima,A.Uchida,and Y.Ishida: "Effect of a feeding attractant,dimethylー ーpropiothetin,on growth of marine fish." Nippon Suisan Gakkaishi. 56. 1151-1154 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Ishida,Y.Ogihara,and S.Okabe: "Effects of a crude extract of a marine dinoflagellate,containing dimethylー ーpropiothetin,on HClーethanol induced gastric lesions and gastric secretion in rats." Japan J.Pharmacol.54. 333-338 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Kawai,Y.Ishida,H.Kakeuchi,N.Ikeda,T.Higashida,and S.Nakamura: "Flavor components of dried squid." J.Agric.Food Chem.

    • Related Report
      1990 Annual Research Report
  • [Publications] A.Uchida: "PRERIMINARY REPORT OF THE HAKUHO MARU CRUISE KHー88ー1" Ocean Research Institute,University of Tokyo, 107 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] B.Kimura: "Phospholipid as a Growth Factor of Uroglena americana,a Red Tide Chrysophyceae in Lake Biwa." Nippon Suisan Gakkaishi. 55. 799-804 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Kawai: "Comparison of Volatile Components of Dried Squid to Reaction Products Formed from the Mixture of Hydrogen Sulfide,Ammonia,and Aldehydes." J.Agr.Food Chem.37. 1026-1031 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Nakajima: "Effect of Supplemental Dietary Feeding Attractant,Dimethylーβーpropiothetin,on Growth of Goldfish." Nippon Suisan Gakkaishi. 55. 1291 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Nishio: "Distribution of IronーSolububilizing Bacteria in the Sediment of a Small lagoon." Nippon Suisan Gakkaishi. 55. 1955-1960 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Ishida: "水産学シリ-ズ77 魚介類の生息環境と着臭" 恒星社厚生閣, 125 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Ishida: "RECENT ADVANCES IN MICROBIAL ECOLOGY" Japan Scientific Societies Press, 724 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 内田有恆: 海洋科学. 120. 74-79 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Shingo Hiroishi: J.Phycol.24. 442-444 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Aritsune Uchida: J.Biochem.Tokyo. 104. 730-734 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Aritsune Uchida: Nippon Suisan Gakkaishi. 54. 1941-1945 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tetsuo Kawai: J.Agr.Food.Chem.

    • Related Report
      1988 Annual Research Report
  • [Publications] Kenji Nakajima: Nippon Suisan Gakkaishi. 55. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Aritsune Uchida: "RED TIDES" ELSEVIER,NEW YORK, 489 (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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