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Purification of IGF-I in Bovine Colostrum and its Application for Animal Production Research.

Research Project

Project/Area Number 63860036
Research Category

Grant-in-Aid for Developmental Scientific Research (B).

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionTohoku University

Principal Investigator

SASAKI Yasuyuki  Tohoku University, Agric. Professor, 農学部, 教授 (90005637)

Co-Investigator(Kenkyū-buntansha) UMEZU Motoaki  Tohoku University, Agric., 農学部, 助手 (30005649)
SHOJI Yoshio  Tohoku University, Agric., 農学部, 助手 (60005642)
KATOH Kazuo  Tohoku University, Agric, Associate Professor, 農学部, 助教授 (60091831)
OHTA Minoru  Tohoku University, Agric, Associate Professor, 附属農場, 助教授 (00005670)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥5,100,000 (Direct Cost: ¥5,100,000)
KeywordsBovine / Colostrum / IGF-I / Purification
Research Abstract

Insulin-like growth factor-I (IGF-I) is a biologically active peptide consisting of 70 amino acids (mol wt. 7,649). Its chemical structure is homologous to proinsulin. The primary structure of IGF-I are almost identical in mammalian species tested (human, rat, mouse, porcine, bovine, ovine).
Biological action of IGF-I, firstly found as sulfation factor, has been shown by recent investigations to stimulate proliferation and differentiation of various cell types. Growth hormone stimulates the secretion of IGF-I, then IGF-I stimulates the growth of young animals through accelerating bone elongation and protein accumulation. On this point IGF-I has been recognized to be of importance for animal production. We showed that the concentration (0.5mg/l) of IGF-I in bovine colostrum exceeded that in blood plasma which has been used as a biological material to extract IGF-I, suggesting availability of colostrum to be an useful material for purifying IGF-I. Colostrum was collected on the day of par … More turition. Chymosin was added to colostrum. After incubation at 37 C, the lipid- and casein-free supernatant was obtained by centrifugation. Acid-ethanol mixture (12.5% HC1, 87.5% EtOH) was added to dissociate IGF-I from its binding protein, followed by further addition of cold acetone to get acetone powder. We used the acetone powder as starting material. The powder was dissolved in 1M acetic acid solution to apply to sephadex G-50 column. Eluate of IGF-I immunoreactive fractions, containing 0.7 ug IGF-I/mg protein, was lyophilized and gel filtration procedure by TSKG3000SW was performed. The TSK column was eluted with 1M acetic acid, IGF-I being purified to 1.6 ug/mg protein. The lyophilized sample was dissolved with 20% acetonitrile in 0.05% TFA, and applied to the first ODP-50 reverse-phase HPLC. IGF-I immunoreactivity was eluted by a two-step linear gradient to 80% acetonitrile. IGF-I concentration of the eluate was 32 ug/mg protein. IGF-I was further purified by the second ODP-50 reverse phase HPLC. The bioassay using rat myoblast subculture cells clearly showed IGF-I bioactivity in the samples eluted from Sephadex and TSK gel column. Less

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] S.Oda: "Insulin like growth factorーI,GH,insulin and glucagon concentrations in bovine colostrm and in plasma of dairy cows and neonatal calves around partition" Comp.Biochem.Physiol.(A). 94. 805-808 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.Oda: "Periparturient changes in concentration of insulin like growth factorーI in bovine colostrum and in plasma of dairy cows and neonatal calves" AsianーAustralasian J.Anim.Sci.2. 210-211 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 小田 伸一: "ウシの分娩に伴う初乳及び血漿インスリン様成長因子ーI、成長ホルモン、インスリン、グルカゴン濃度変動について" 川渡農場報告. 5. 63-66 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 小田 伸一: "ウシの分娩に伴う血漿および初乳IGFーI、GH、インスリン、グルカゴン濃度変動について" 第80回日本畜産学会大会講演要旨. 185 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 小田 伸一: "ウシ初乳インスリン様成長因子ーIの調製について" 第84回日本畜産学会大会・大会要旨. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oda S.: "Insulin like growth factor-I, GH, insulin and glucagon concentrations in bovine colostrum and in plasma of dairy cows and neonatal calves around parturition." Comp. Biochem. Physiol.94 (A). 805-808 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oda S.: "Periparturient changes in concentration of insulin like growth factor-I in bovine colostrum and in plasma of dairy cows and neonatal calves." Asian-Australasian J. Anim. Sci.2. 210-211 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oda S.: "Insulin like growth factor-I, GH, insulin and glucagon concentrations in bovine colostrum and in plasma of dairy cows and neonatal calves around parturition. (in Japanese)" Bulletin of The Exp. Farm, Tohoku Univ.5. 63-66 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oda S.: "IGF-I, GH, insulin and glucagon concentrations in bovine colostrum and in plasma of dairy cows and neonatal calves around parturition." 80st Annual Meeting Program-handbook of Jpn. Soc. Zooteck. Sci.185 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oda S.: "Purification of IGF-I from bovine colostrum." 84st Annual Meeting program-handbook of Jpn. Soc. Zooteck.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.Oda: "Insulin like growth factorーI,GH,insulin and glucagon concentrations in bovine colostrum and in plasma of dairy cows and neonatal calves around parturition" Comp.Biochem.Physiol.(A). 94. 805-808 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Oda: "Periparturient changes in concentration of insulin like growth factorーI in bovine colostrum and in plasma of dairy cows and neonatal calves" AsianーAustralasian J.Anim.Sci.2. 210-211 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小田 伸一: "ウシの分娩に伴う初乳及び血漿インスリン様成長因子ーI、成長ホルモン、インスリン、グルカゴン濃度変動について" 川渡農場報告. 5. 63-66 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小田 伸一: "ウシの分娩に伴う血漿および初乳IGFーI、GH、インスリン、グルカゴン濃度変動について" 第80回日本畜産学会大学講演要旨. 185 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小田 伸一: "ウシ初乳インスリン様成長因子ーIの調製について" 第84回日本畜産学会大会・大会要旨. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Oda S.: "Periparturient changes in concentration of insulin like growth factor-I in bovine colostrum and plasma of dairy cows and neonatal calves." Asian-Australasian J.Anim.Sci.2. 210-211 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Oda S.: "Insulin like growth factor-I,GH,insulin and glucagon concentrations in bovine colostrum and in plasma of dairy cows and neonatal calves around parturition" Comp.Biochem.Physiol.94A. 805-808 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 小田伸一: "ウシの分娩に伴う血漿および初乳IGF-I、GH、インスリン、グルカゴン濃度変動について" 第80回日本畜産学会大会講演要旨集. 185 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 小田伸一: 第80回日本畜産学会大会講演要旨集. 185 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Oda: Asian-Australasian J.Anim.Sci. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Oda: Brit.J.Nutr.

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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