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STUDY ON THE DEVELOP OF IDIOTYPIC ANTIBODY TO ESTROGEN AND ON THE APPLICATION IT TO CLINICAL USE

Research Project

Project/Area Number 63870003
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionJIKEI UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF MEDICINE

Principal Investigator

ISHIKAWA Hiroshi  JIKEI UNIV. SCHL. MED. MEDICINE PROFESSOR, 医学部, 教授 (30089784)

Co-Investigator(Kenkyū-buntansha) GON Gotetsu  JIKEI UNIV. SCHL. MED. MEDICINE ASSIST. PROF., 医学部, 助手 (40170477)
SHIRASAWA Nobuyuki  JIKEI UNIV. SCHL. MED. MEDICINE LECTURER, 医学部, 講師 (40133392)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1989: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1988: ¥3,000,000 (Direct Cost: ¥3,000,000)
KeywordsESTROGEN / ESTROGEN ANTIBODY / IDIOTOPE / IDIOTYPIC ANTIBODY / ESTROGEN RECEPTOR / MONOCLONAL ANTIBODY / HYBRIDOMA / ESTROGEN SYNTHESIS / エストロゲン抗体 / 細胞融合 / イヂオタイプ抗体 / モノクローナル抗体 / エストロゲン受容体
Research Abstract

In 1974, Niels Jerne proposed a theory which pictured the immune system as a network of interacting idiotypes. This network theory of immune response emphasized the need to consider individual immunoglobulin molecules as expressing two critical sets through which they interacted with other elements of the immune system. Each molecule bears a combining site through which it interacts with conventional antigenic determinants and with other immunoglobulin molecules which bore idiotypic determinants cross-reacting with antigen. Jerne designated the antigen-combining sites of immunoglobulins as paratopes. The antigenic determinants of conventional antigens are referred to as epitopes. Each immunoglobulin molecule expresses one, or many, antigenic determinants determined by the structure of its variable regions and thus unique to it, or at least shared by only a relatively limited number of immunoglobulin molecules. These idiotypic determinants were termed as idiotopes. on the bases on these … More situation, we tried to make idiotypic antibodies that bind to estrogen receptor.
We speculated that some of them should have biological activity as estrogen has. The idiotypic antibodies would be a good tool to analyze estrogen receptors in cells and be a useful medicine, which are immunoglobulin but not steroid structure, for clinical fields. First of all, we made polyclonal and monoclonal antibodies to estrogen. Then the estrogen antibodies were injected to mice to rise idiotypic antibody, that is, anti-anti-estrogen immunoglobulin. After giving several boosters, mouse spleen cells were collected and fused with mouse myeloma cells to make hybridoma producing the idiotypic antibody. The idiotypic activity was checked by the enzyme-linked immunosorbant assay (ELISA) and the binding inhibition of ^3H-estrogen to estrogen antibody. We tried several cell fusion and got thousands of hybridomas and more than one hundred monoclonal cells producing substances having estrogen like activity. We further more analyzed the biological, biochemical and chemical characteristics of these substances. The biological activity using in vivo and in vitro showed estrogen-like activity, however, biochemical analysis showed that the substance was not immunoglobulins and was lower molecular weight chemicals. We finally concluded that the substance was estrogen itself by HPLC and mass spectrography.
These finding is very exciting one that high percentages of hybridoma have an ability to synthesize estrogen in vitro. Less

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (52 results)

All Other

All Publications (52 results)

  • [Publications] Gon,G.: "Granular,ciliated cells in the anterior pituitaries of immature rats." Cell Tissue Research.253. 683-684 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Gon,G.: "Formation of 'mixed follicles'in anterior pituitary of perinatal rat---with special reference to follicular growth hormone cells---." Jikeikai Med.J.35. 641-647 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shirasawa,N.: "Comparative immunohistochemical study of the mammarian pituitary cells stained with antisera against S-100 protein and non-neuronal enalase." Biomedical Research. 9. 477-487 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shirasawa,N.: "Ultrastructural classification of follicular cells and their relation in bovine pituitary glands." Biomedical Research.9. 503-514 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shimada,T.: "Dome formation of Keratincontaining granular cells from rat anterior pituitary gland in vitro." In Vitro Cellular & Development Biology. 24. 1193-1200 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sako,T.: "Glucagon-like material in the human and bovine pituitaries as revealed by immunochemistry." Jikei Med.J.36. 21-27 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shirasawa,N.: "Comparative immunohistochemical studies of prolactin(PRL)and growth hormone(GT)cells in mammals." Kyoto Prolactin Conference.4. 20-32 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nogami,H.: "Studies on Prolactin and Growth Hormone Gene Expression in the Pituitary Gland of Spontaneous Dwarf Rats." Endocrinology. 125. 964-970 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ishiwata,I: "Bioiogical characteristics of adenocarcinoma cell line (HOUA-I)and B-lymphoblastoid cell line(HOUA-II)established from the same tissue of endometrial adenocarcinoma" Jikeikai Medical Journal. 36(4). 303-315 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fukuchi,Y.: "Growth hormone increases the antral gastrin cell density in spontaneous rats." Biomedical Research. 10(6). 485-490 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ishiwata,I.: "N-myc Amplification and Neuron-Specific Enolase Production of Ovarian Immature Teratoma" ACTA OBST GYNAEC JPN. 41. 1585-1586 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ono,I.: HUMAN CELL. 2(4). 416-422 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Enomoto,H: "Formation of secretory granules and suppression of prolactin secretion in agranular prolactin-secreting clonal cells by calmodulin inhibitors in vitro."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nogami,H.: "Studies on the development of growth hormone and prolactin cells in the rat pituitary gland by in situ hybridization." Cell Tissue Research.255. 23-28 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nogami,H.: "Development of the prolactin and growth hormone cells in the rat anterior pituitary gland:immunocytochemical and in situ hybridization study." Kyoto prolactin conference monograph. 3. 51-60 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ishiwata,I.: "Changes in leukocytes count in peripheral blood of nude mouse bearing transplanted gynecological malignant tumors." Acta.Obst.Gynec.Jpn.40. 1767-1771 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ishiwata,I.: "Glandular cell properties of cell line(HKUS)derived from small cell non-keratinizing squamous cell carcinoma of the uterine cervix" Acta.Obst.Gynec.Jpn.40. 616-620 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sakuma,S.: "Immunohistochemical studies on the somatostatin-and growth hormone-relating factor(GRF)-neurons in the hypothalamus of the novel dwarf rat:the spontaneous dwarf rat(dr)." Brain Research.493. 402-406 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nogami,H.: "Electron-microscopic study on the anterior pituitary gland of spontaneous dwarf rats" Cell and Tissue Research. 258. 477-487 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] J.H.Steel: "Galanin and Vasoactive intestinal polypeptide are colocalised with classical pituitary hormones and show plasticity of expression" Histochemistry. 93. 183-189 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sakuma,S.: "The cell population of somatostatin and gorwth hormone releasing factor using quantitative immunohistochemistry in the isolated GH deficient dwarf rat" Brain Research. 506. 307-310 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takeuchi,T.: "Molecular Mechanism of Growth Hormone(GH)Deficiency in the Spontaneous Dwarf Rat:Detection of Abonormal Splicing of GH mRNA by the polymerase Chain Reaction." Endocrinology. 126(1). 31-38 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Dhruba Raj Naik: "Localization of Prolactin in the Pituitary Gland:a Comparative immunohistochemical Study from Fish to Mammal." Kyoto Prolactin Conference.in press. 5.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ishiwata,I,: "Carcina-embryonic proteins produced by Wilm's tumor cells in vitro and in vivo." Exp.Pathol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ishiwata,I: "Characterization of Krukenberg tomor cell line especially biological relationship between cancer and stormal cell." Esp.Pathol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 石川博: "ファ-マコバイオサイエンス・薬学生のための生物学(ヒトの発生の項分担執筆)" 広川書店, 96-129 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 石川博: "現代病理学大系17A 石川英世、他編(甲状腺構造、発生、発生分化の項分担執筆)" 中山書店, 261-273 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 石川博: "介護福祉士実践シリ-ズ3巻 老人介護の医学知識 「介護福祉教材」編集委員会編(第2章p25-p194)" メジカルフレンド社, 328 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 石川博: "コアテキスト組織学 p311-p339,p358-p362,p392-p403" 広川書店, (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 石川博: "臨床検査技師国家試験要点整理問題演習4 解剖学 p115-p215" 金原出版, 301 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shirasawa,N.: "Comparative immunohistochemical studies of prolactin(PRL)and growth hormone(GH)cells in mammals." Kyoto prolactin Conference.4. 20-32 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nogami.H.: "Studies on prolactin and Growth Hormone Gene Expression in the Pituitary Gland of spontaneous Dwarf Rats." Endocrinology. 125. 964-970 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fukuchi,Y.: "Growth hormone increases the antral gastrin cell density in spontaneous dwarf rats." Biomedical Research.10(6). 485-490 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ishiwata,I.: "N-myc Amplification and Neuron-Specific Enolase Production of Ovarian Immature Teratoma" ACTA OBST GYNAEC JPN. 41. 1585-1586 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ishiwata,I.: "Biological characteristics of adenocarcinoma cell line(HOUA-I)andB-lymphoblastoid cell line(HOUA-II)established from the same tissue of endometrial adenocarcinoma" Jikeikai Medical Journal. 36(4). 303-315 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sakuma,S.: "Immunohistochemical studies on the somatostatin-and growth hormone-relating factor(GRF)-neurons in the hypothalamus of the novel dwarf rat;the spontaneous dwarf rat(dr)." Brain Research.493. 402-406 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nogami,H.: "Electron-microscopic study on the anterior pituitary gland of spontaneous dwarf rats" Cell and Tissue Research. 258. 477-487 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] J.H.Steel: "Galanin and Vasoactive intestinal polypeptide are colocalized with classical pituitary hormones and show plasticity of expression" Histochemistry. 93. 183-189 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ono,I.: "神経芽腫細胞株HSNBの樹立と性状" HUMAN CELL. 2(4). 416-422 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Takeuchi.T.: "Molecular Mechanism of Growth Hormone(GH)Deficiency in the Spontaneous Dwarf Rat:Detection of Abnormal Splicing of GH mRNA by the polymerase Chain Reaction." Endocrinology. 126. 31-38 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sakuma,S.: "The cell population of somatostatin and growth hormone releasing factor using guantitative immunohistochemistry in the isolated GH deficient dwarf rat" Brain Research. 506. 307-310 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ishiwata,I.: "Carcina-embryonic proteins produced by Wilm's tumor cells in vitro and in vivo." Exp.Pathol.

    • Related Report
      1989 Annual Research Report
  • [Publications] Ishiwata,I.: "Characterization of Krukenberg tumor cell line especially biological relationship between cancer and stromal cell." Esp.Pathol.

    • Related Report
      1989 Annual Research Report
  • [Publications] Dhruba Raj Naik: "Localization pf Prolactin in the Pituitary Gland:a Comparative Immunohistochemical Study from Fish to Mammal." Kyoto Prolactin Conference.5.

    • Related Report
      1989 Annual Research Report
  • [Publications] 石川博: "介護福祉士実践シリ-ズ3巻老人介護の医学知識「介護福祉教材」編集委員会編" メジカルフレンド社, 328 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 石川博: "コアテキスト組織学(P311-P339,P358-P362,P392-P403)" 広川書店, 517 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 石川博: "臨床検査技師国家試験要点整理問題演習4解剖学" 金原出版, 301 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shirasawa,N.;et al.: Biomedical Research. 9. 477-487 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Shirasawa,N.;et al.: Biomedical Research. 9. 503-514 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Gon,G.;et al.: Cell Tissue Research. 253. 683-684 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Gon,G.;et al.: Jikeikai Medical Journal. 35. 641-647 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Enomoto,H.;et al.: Jikeikai Medical Journal. 35. 441-454 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2017-10-19  

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