| Project/Area Number |
63870008
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
SHINODA Yoshikazu Tokyo Medical and Dental Univ., Dept. of Physiol., School of Medicine, Professor., 医学部, 教授 (60010104)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1989: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1988: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Keywords | Reconstruction device / Neuron / Microscope / Golgi staining / HRP staining / 形態解析 / 三次元再構築 / 三次元コンピュ-タ-解析描画装置 / 三次元コンピュ-ター解析描画装置 / HRP / 前庭核 / 脊髄 / 小脳 / 細胞内染色 |
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| Research Abstract |
To accelerate the speed of analysis on three dimensional structures of single neurons, a new reconstruction device was developed. This device was composed of a conventional microscope with a camera lucida drawing attachment, a motor-driven microscope stage and a motor-driven table which can be moved in X Y directions. The motor-driven tables moves in proportion to the movement of the motor-driven stage by a microprocessor system, so that the desired structures on the brain slice under a microscope can be drawn through the drawing attachment and then, by moving the neighboring structures just outside the view of the microscope in to the viewer, those structures can be described continuously. By repeating this procedure, the whole structure of a single neuron on one slice can be traced. Serial sections are processed in the same way. Finally, each drawing of one brain section adjusted one by one to connect cut ends of neuronal structures of one slice to the appropriate cut ends on the previous slice. This device can trace a neuronal structure and enlarge it to 1.5m wide x 2 m long. The reconstruction time is speeded by three times.
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