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Electron microscopic immunocytochemistry of protein kinase C using subspecies-specific anti-peptide antibody

Research Project

Project/Area Number 63870011
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionKobe University School of Medicine

Principal Investigator

TANAKA Chikako  Kobe Univ.Sch.Med., Dept. of Pharmacol., Professor, 医学部, 教授 (20025571)

Co-Investigator(Kenkyū-buntansha) SAITO Naoaki  Kobe Univ.Sch.Med., Dept. of Pharmacol., Research Associate, 医学部, 助手 (60178499)
KUNO Takayoshi  Kobe Univ.Sch.Med., Dept. of Pharmacol., Assistant Professor, 医学部, 講師 (50144564)
TANIYAMA Kohtaro  Kobe Univ.Sch.Med., Dept. of Pharmacol., Associate Professor, 医学部, 助教授 (70030898)
NISHIZUKA Yasutomi  Kobe Univ.Sch.Med., Dept. of Biochem., Professor, 医学部, 教授 (10025546)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 1989: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1988: ¥10,200,000 (Direct Cost: ¥10,200,000)
KeywordsProtein kinase C / Subspecies / Cloning / Central nervous system / Immunocytochemistry / Electron microscopy / プロテインキナーゼC / クローニング
Research Abstract

Protein kinase C (PKC) is a key enzyme involved in the signal transduction mechanism of many physiological processes. The enzyme is now known to exist as a family of several subtly different, but closely related subspecies. Thus far several laboratories have isolated cDNA clones encoding distinct forms of PKC, namely, alpha, betaI, betaII, gamma, delta, epsilon, and zeta. PKC subspecies exhibit subtle difference in their enzymological characteristics and cellular locations, suggesting they have distinct physiological roles. We synthesized subspecies-specific oligopeptides for alpha, betaI, betaII, and gamma-PKC and obtained subspecies-specific antibodies by immunizing rabbits with the oligopeptides. We aimed in this project to elucidate the functional difference between the subspecies of PKC in nervous tissue using electron microscopic immunocytochemistry. The distribution of each PKC subspecies was distinctly different and the distributions did not completely correspond with those of any neurotransmitters and receptors. Under electron microscopy, gamma-PKC was present in the perikaryon, plasma membrane, nucleus, dendrite, and axon. betaI-PKC was just adjacent to plasma membrane, while betaII-PKC was located around Golgi complex. In the hippocampus, gamma-PKC was distributed diffusely throughout the cytoplasm of pyramidal cells from the perikarya to dendritic spine. In contrast, betaII-PKC was concentrated around Golgi complex and present diffusely in distal dendrites, except for the dendritic spines. Neither PKC subspecies could not be detected in the synaptic terminals. These results suggest that each PKC subspecies has a specific function in the neurons and that gamma-PKC and betaII-PKC are involved in long term potentiation postsynaptically in the hippocampus.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] A.Ito: "Immuoncytochemical localization of α-subspecies of protein kinase C in rat brain." Proc.Natl.Acad.Sci.USA. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Hosoda: "Immunocyutochemical localization of the β1-subspecies of protein kinase C in rat brain." Proc.Natl.Acad.Sci.USA. 86. 1393-1397 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N.Saito: "Immunocytochemical localization of β11-subspecies of protein kinase C in rat brain." Proc.Natl.Acad.Sci.USA. 86. 3409-3431 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] A.Ito: "Localization of β11-subspecies of protein kinase C in B cell of pancreatic islet." Diabetes. 38. 1005-1011 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Tsujino: "Light and electron microscopic localization of β1-,β11-and γ-subspecies of protein kinase C in rat cerebral cortex." J.Neurosci. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] A.Kose: "Flectron microscopic localization of γ-and β11-subspecies of protein kinase C in rat hippocampus." Brain Res.(1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] A. Ito: "Immunocytochemical localization of alpha-subspecies of protein kinase C in rat brain." Proc. Natl. Acad. Sci. USA. (1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K. Hosoda: "Immunocytochemical localization of the betaI-subspecies of protein kinase C in rat brain." Proc. Natl. Acad. Sci. USA 86 (1989), 1393-1397.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N. Saito: "Immunocytochemical localization of betaII-subspecies of protein kinase C in rat brain." Proc. Natl. Acad. Sci. USA. 86 (1989), 3409-3431.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] A. Ito: "Localization of betaII-subspecies of protein kinase C in B cell of pancreatic islet." Diabetes 38 (1989), 1005-1011.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T. Tsujino: "Light and electron microscopic localization of betaI-, betaII- and gamma-subspecies of protein kinase C in rat cerebral cortex." J. Neurosci. (1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] A. Kose: "Electron microscopic localization of gamma- and betaII- subspecies of protein kinase C in rat hippocampus." Brain Res. (1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] A.Ito: "Immunocytochemical localization of α-subspecies of protein kinase C in rat brain." Proc. Natl.Acad Sci. USA.inpress. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Hosoda: "Immunocytochemical localization of βI-subspecies of protein kinase C in rat brain." Proc. Natl.Acad Sci. USA.86. 1393-1397 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] N.Saito: "Immunocytochemical localization of βII-subspecies of protein kinase C in rat brain." Proc. Natl.Acad Sci. USA.86. 3409-3431 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] A.Ito: "Localization of βII-subspecies of protein kinase C in B cell of pancreatic islet." Diabetes. 38. 1005-1011 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Tsujino: "Light and electron microscopic localization of βI-,βII-and γ-subsepecies of protein kinase C in rat cerebral cortex." J.Neurosci.in press. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] A.Kose: "Flectron microscopic localization of γ-and βII-subspecies of protein kinase C in rat hippocampus." Brain Res.in press. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] N.Saito: Pro.Natl.Acad.Sci.USA. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Hosoda: Pro.Natl.Acad.Sci.USA. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] A.Kose: J.Neurosci.8. 4262-4268 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.Hosoda: J.Neurosci.8. 1678-1683 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] N.Saito: J.Neurosci.8. 369-382 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Ase: J.Neurosci.8. 3850-3856 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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