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THE DEVELOPMENT OF LASER-FLUOROMETRIC MICROSCOPE FOR MEASUREMENT OF INTRACELLULAR CALCIUM IONS SINGLE LIVING CELLS.

Research Project

Project/Area Number 63870038
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionTOKYO UNIVERSITY, FACULTY OF MEDICINE.

Principal Investigator

YAZAKI Yoshio  UNIVERSITY OF TOKYO, MEDICINE, ASSOCIATE PROFESSOR., 医学部第三内科(病), 助教授 (20101090)

Co-Investigator(Kenkyū-buntansha) INAMOTO Naoki  OHTSUKA ELECTRONICS, 研究員
OKA Kohichi  OHTSUKA ELECTRONICS, 主任
YOSHIZUMI Masao  UNIVERSITY OF TOKYO, MEDICINE, 医学部第三内科(病), 医員
SUGIYAMA Takao  UNIVERSITY OF TOKYO, MEDICINE, 医学部第三内科(病), 医員
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥24,000,000 (Direct Cost: ¥24,000,000)
Fiscal Year 1989: ¥9,100,000 (Direct Cost: ¥9,100,000)
Fiscal Year 1988: ¥14,900,000 (Direct Cost: ¥14,900,000)
KeywordsINTRACELLULAR CALCIUM IONS / HYPERTENSION / LASER MICROSCOPE / VASCULAR SMOOTH MUSCLE CELLS / INDO-1 / SPONTANEOUSLY HYPERTENSIVE RATS / ABNORMAL CALCIUM HANDLING / INTRACELLULAR CALCIUM ION ELEVATING FACTOR / インドー1(INDC-1) / レーザー螢光顕微鏡 / Indoー1 / 血管平滑筋 / SHR / WKYラット
Research Abstract

We have developed an accurate and sensitive system for the measurement of cytoplasmic free calcium concentrations ([Ca^<++>]_i) of a single vascular smooth muscle cell (VSMC) by using UV-laser and Indo-1. [Ca^<++>]_i in spontaneously hypertensive rat (SHR) was elevated and maintained after the 6th passage culture. And moreover, marked elevation of [Ca^<++>]_i after stimulation with caffeine and angiotensin-II occurred in VSMC in some populations of 4-week-old SHR even when blood pressure and basal [Ca^<++>]_i had not yet been elevated. In 8-week-old rats, the basal [Ca^<++>]_i from SHR was elevated compared with Wistar Kyoto rats. We have attempted to characterize yet unspecified circulating factor(s) from SHR serum that elevates [Ca^<++>]_i in VSMC. Our results showed that 1) the maintenance of high [Ca^<++>]_i levels in VSMC in SHR is genetically regulated and it is one of the mechanisms in SHR hypertension, 2) abnormal calcium regulation in VSMC from SHR is expressed even prior to overt hypertension, and 3) the [Ca^<++>]_i elevating factor(s) exists in 8W-SHR serum, but not in 4W-SHR, 4W- or 8W- WKY serum.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Komuro I.Kurihara H.他: "Endothelin stimulate c-fos and c-myc expression and proliferation of vascular smooth muscle cells." FEBS Lett.238. 249-252 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yanagisawa M.Kurihara H.他: "A novel potent vasoconstrictor peptide produced by vascular endothelial cells." Nature.332. 411-415 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kurihara H.Yoshizumi M: "The possible role of endothelin-1 in the pathogenesis of coronary vasospasm." J Cardiovascular Pharmacol.13(Supl.5). S132-S137 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kurihara H.Yoshizumi M: "Transforming growth factor-β stimulates the expression of endothelin mRNA by vascular endothelial cells." Biochem Biophys Res Commun.159. 1435-1440 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yoshizumi M.Kurihara H.: "Hemodynamic shear stress stimulates endothelin production by cultured endothelial cells." Biochem Biophys Res Commun.161. 859-864 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sugiyama T.Yoshizumi M.: "Abnormal calcium handling in vascular smooth muscle cells in spontaneously hypertensive rats." J.Hypertens.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Komuro I, Kurihara H, Sugiyama T, Yoshizumi M, Takaku F, Yazaki Y.: "Endothelin stimulate c-fos and c-myc expression and proliferation of vascular smooth muscle cells." FEBS Lett.238. 249-252 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y, Yazaki Y, Goto K, Masaki T.: "A novel potent vasoconstrictor peptide produced by vascular endothelial cells." Nature.332. 411-415 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kurihara H, Yoshizumi M, Sugiyama T, Yamaoki K, Nagai R, Takaku F, Satoh H, Inui J, Yanagisawa M, Masaki T, Yazaki Y.: "The possible role of endothelin-1 in the pathogenesis of coronary vasospasm." J Cardiovascular Pharmacol.13(Sup 5). 132-137 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kurihara H, Yoshizumi M, Sugiyama T, Takaku F, Yanagisawa M, Masaki T, Hamaoki M, Kato H, Yazaki Y.: "Transforming growth factor-beta stimulates the expression of endothelin mRNA by vascular endothelial cells." Biochem Biophys Res Commun. 159. 1435-1440 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yoshizumi M, Kurihara H, Sugiyama T, Takaku F, Yanagisawa M, Masaki T, Yazaki Y.: "Hemodynamic shear stress stimulates endothelin production by cultured endothelial cells." Biochem Biophys Res Commun. 161. 859-864 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sugiyama T, Yoshizumi M, Takaku F, Yazaki Y.: "Abnormal calcium handling in vascular smooth muscle cells in spontaneously hypertensive rats." J Hypertens.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Komuro I,Kurihara H,他: "Endothelin stimulate c-fos and c-myc expression and proliferation of vascular smooth muscle cells." FEBS Lett.238. 249-252 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yanagisawa M,Kurihara H,他: "A novel potent vasoconstrictor peptide produced by vascular endothelial cells." Nature.332. 411-415 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kurihara H,Yoshizumi M: "The possible role of endothelin-1 in the pathogenesis of coronary vasospasm." J Cardiovascular Pharmacol.13(Supl.5). S132-S137 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kurihara H,Yoshizumi M,: "Transforming growth factor-β stimulates the expression of endothelin mRNA by vascular endothelial cells." Biochem Biophys Res Commun.159. 1435-1440 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshizumi M,Kurihara H,: "Hemodynamic shear stress stimulates endothelin production by cultured endothelial cells." Biochem Biophys Res Commun.161. 859-864 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sugiyama T,Yoshizumi M,: "Abnormal calcium handling in vascular smooth muscle cells in spontaneously hypertensive rats." J Hypertens.

    • Related Report
      1989 Annual Research Report
  • [Publications] Mashiko-Kurabayashi,et al.: J.Biol.Chem.263. 13930-13936 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hidetsugu-Tsuchimochi,et al.: Circulation. 78. 920-927 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Masahiko-Kurabayashi,et al.: J.Clin.Invest.82. 524-531 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Issei-Komuro,et al.: Circ.Res.62. 1075-1079 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kazuomi-Nomoto,et al.: Circ.Res.62. 1088-1092 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Masashi-Yanagisawa,et al.: Nature. 332. 411-415 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yoshio-Yazaki,et al.: "Distribution of cardiac myosin isozymes in human and rat heartimmunohistochemical study using monoclonal antibodies.Heart function and metabolism." Martinus Nijhoff Publishing,Boston, 293-305 (1987)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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