| Project/Area Number |
63870100
|
|---|
| Research Category |
Grant-in-Aid for Developmental Scientific Research (B).
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
医学一般
|
|---|
| Research Institution | University of Tokyo |
|---|
Principal Investigator |
SUGIMOTO Tsuneaki University of Tokyo, Faculty of Medicine, Professor, 医学部(病), 教授 (60019883)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Toshiaki University of Tokyo, Faculty of Medicine, Associate, 医学部(病), 助手 (50227790)
IINO Masamitsu University of Tokyo, Faculty of Medicine, Associate, 医学部, 助手 (50133939)
MARUYAMA Yoshio Jichi Medical University, School of Medicine, Assoc. Prof., 医学部, 助教授 (00133942)
TANAKA Hiroshi Hamamatsu Medical University, Medical Information, Assoc. Prof., 医療情報部, 助教授 (60155158)
TOYOOKA Teruhiko University of Tokyo, Health Administration, Assoc. Prof., 保健センター, 助教授 (00146151)
倉智 嘉久 東京大学, 医学部(病), 助手 (30142011)
|
|---|
| Project Period (FY) |
1988 – 1990
|
| Project Status |
Completed (Fiscal Year 1990)
|
| Budget Amount *help |
¥28,800,000 (Direct Cost: ¥28,800,000)
Fiscal Year 1990: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1989: ¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 1988: ¥14,400,000 (Direct Cost: ¥14,400,000)
|
|---|
| Keywords | Cellular electrical activities / cellular function analysis / atrial myocyte / bronchial smooth muscle cell / intestinal smooth muscle cell / pancreatic acinar cell / intracellular Ca dynamics / vascular smooth muscle cell / 細胞機能解析 / 血管平滑筋細胞 / 腸評滑筋細胞 / 細胞Ca動態 / 細胞内カルシウム測定装置 / パッチクランプ法 / 単一組織細胞 / 興奮ー機能連関 / 興奮一機能連関 |
|---|
| Research Abstract |
The study was performed to establish the novel method to investigate the cellular excitation-function coupling by means of simultaneous recording of cellular electrical, mechanical as well as neurohumoral activities.
1. The electrical activities and function : We clarified the role of arachidonic acid and their metabolites in the regulation of the muscarinic K channel in single pacemaker and atrial cells. In isolated tracheal smooth muscle cells, the effects of several agents on the contraction and the ionic currents were studied. In ileum cells, the ionic basis of the agents, which affect the bowel movement was clarified. In pancreatic acinar cells, we investigated the relations between the stimuli and secretion in the cells by recording the alterations of membrane capacitance of the cells which reflect the exocytosis function.
2. Dynamics and function of the intracellular Ca store : In the smooth muscle cells isolated from cecum, there was some delay between the time-integrated fluorescent density and the Ca current. The relation between the velocity of the Ca leak and Ca uptake suggested that there are several compartments in sarcoplasmic reticulum.
3. Multidimensional image analysis of the intracellular Ca distribution : In vascular smooth muscle cells, the distributions of caffeine and IP-3 sensitive Ca channels were different among cells and were attributed to the phase of cell growth cycle.
|
