• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Production of monoclonal

Research Project

Project/Area Number 63880018
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionThe University of Tokyo

Principal Investigator

KOBATA Akira  Inst. of Med. Sci., Dept. of Biochem., Professor, 医科学研究所, 教授 (30030852)

Co-Investigator(Kenkyū-buntansha) KITAJIMA Toru  Towa Kasei Co., Ltd., Research Inst., Section Chief, 研究所, 係長
YASUDA Tatsuji  Inst. Environ. Med., Okayama Univ. Med. Sch., Professor, 医学部, 教授 (30092357)
KATAGIRI Takuya  Inst. of Med. Sci., Dept. of Immunol., Research Association, 医科学研究所, 助手 (70126100)
北村 徹  東和化成工業, 富士研究所, 係長
狩野 恭一  東京大学, 医科学研究所, 教授 (80152825)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥13,900,000 (Direct Cost: ¥13,900,000)
Fiscal Year 1989: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1988: ¥9,800,000 (Direct Cost: ¥9,800,000)
KeywordsMonoclonal antibody / IgG / Abnormal Biantennary / Anti NCA-2 antibody / Type I sugar chain / Asialo GM1 / Epitope / Lipid A / 単クロ-ン抗体 / 免疫染色 / Le^a抗原決定基 / CEA / NFAー2 / Nー結合糖鎖 / 単クローン抗体 / アスパラギン結合糖鎖 / 人工糖タンパク質 / リポソーム
Research Abstract

1. Production of Monoclonal Antibody Directed to the N-linked Sugar Chains of Glycoproteins. Chemical synthesized Galbeta1->4GIcNAcbeta1->4Manalpha1->6(Manalpha1->3)Manbeta1->O-(CH_2)_8-COOH was coupled to methylated BSA. Monoclonal antibodies made against this artificial glycoprotein were screened with poly-D-lysine to which the above pentasaccharide derivative was coupled. From approximately 500 samples, three antibodies were confirmed to react with the above pentasaccharide. Therefore, the method will be effectively used to make monoclonal antibodies directed to N-linked sugar chains.
2. Epitope Determination of Monoclonal Antibodies Directed to the Sugar Chains of Glycoproteins. A novel sensitive method to determine the epitope of antibodies directed to the sugar chains of glycoproteins was established. In principle, the method is the immunostaining of oligosaccharides chemically coupled to an amino-inpregnated TLC plate by reduction with NaBH_3CN.
3. Basic Studies for The Effective Production of IqG-type Monoclonal Antibodies Directed to Sugars. It was found that an autoimmune mouse MRL/Ipr produces IgG-type anti-asialo GM 1 monoclonal antibodies in approximately twenty times higher rate than normal mice. This result indicates that the mouse will be a useful animal for the production of IgG-type monoclonal antibodies directed to sugar chains.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Yamashita,K.Totani,K.,Kuroki,M.,Matsuoka,Y.,Endo,T.,Kobata,A.: "Carbohydrate structures of nonspecific cross-reacting antigen-2,a glycoprotein purified from meconium as an antigen cross-reacting with anti CEA antibody." J.Biol.Chem.264. 17873-17881 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohbayashi,H.,Endo,T.,Yamashita,K.,Kuroki,M.,Matsuoka,Y.,Kobata,A.: "Novel methods to determine the epitopes on the asparagine-linked oligosaccharides of glycoproteins." Anal.Biochem.182. 200-206 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Umeda,M.,Tomita,T.,Shibata,H.,Seki,M.,Yasuda,T.: "Homogeneous liposome lysis assay for determination of anti-strepto-lysin O antibody titre in serum." J.Clinical Microbiol.26. 804-807 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hosoda,K.,Yasuda,T.: "Homogeneous immunoassay for alpha-2 plasmin inhibitor and alpha-9PI-plasmin complex." J.Immunol.Methods. 121. 121-128 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Katagiri,T.,Ting,J.P.,Dy,R.,Prokop,C.,Cohen,P.,Earp,H.S: "Tyrosine phosphorylation of a c-Src-like protein is increased in membranes of CD4^-CD8^-T lymphocytes from lpr/lpr mice." Mol.Cell.Biol.9. 4914-4922 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Katagiri,T.,Urakawa,K.,Yamanashi,Y.,Semba,K.,Takahashi,T.,Kato,K.: "Overepression of src family gene for tyrosine-kinase p59^<fyn> in CD4^-CD8^-T cells of mice with a lymphoproliferative disorder." Proc.Natl.Acad.Sci.86. 10064-10068 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kobata,A.,Furukawa,K.: "Handbook on glycoproteins" Marcel Dekker.Inc.New York, (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yasuda,T.,Ishimori,Y.,Umeda,M.: "Liposome Immune Lysis Assay:Non-isotopic Immunoassay" Plenum Press, 389-399 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yamashita, K., Totani, K., Kuroki, M., Matsuoka, Y., Endo, T., Kobata, A.: "Carbohydrate structures of nonspecific cross-reacting antigen-2, a glycoprotein purified from meconium as an antigen cross-reacting with anti CEA antibody." J.Biol.Chem.264-30. 17873-17881 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohbayashi, H., Endo, T., Yamashita, K., Kuroki, M., Matsuoka, Y., Kobata, A.: "Novelmethods to determine the epitopes on the asparagine-linked oligosaccharides of glycoproteins." Anal.Biochem.182-2. 200-206 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Umeda, M., Tomita, T., Shibata, H., Seki, M., Yasuda, T.: "Homogeneous liposome lysis assay for determination of antistereptolysin O antibody titre in serum." J. Clinical Microbiol.26. 804-807 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hosoda, K., Yasuda, T.: "Homogeneous immunoassay for alpha-2-plasmin inhibitor and alpha-2Pl-plasmin complex." J. Immunol.Methods. 121. 121-128 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Katagiri, T., Ting, J.P., Dy, R., Prokop, C., Cohen, P., Earp, H.S.: "Tyrosine phosphorylation of a c-Src-like protein is increased in membranes of CD4^-CD8^- T lymphocytes from lpr/lpr mice." Mol.Cell.Biol.9. 4914-4922 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Katagiri, T., Urakawa, K., Yamanashi, Y., Semba, K., Takahashi, T., Kano, K.: "Overexpression of src family gene for tyrosine-kinase p59^<fyn> in CD4^-CD8^- T cells of mice with a lymphoproliferative disorder." Proc.Natl.Acad.Sci.86-24. 10064-10068 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kobata, A., Furukawa, K.: Handbook on glycoproteins. Marcel Dekker, Inc., New York, (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yasuda, T., Ishimori, Y., Umeda, M.: Liposome Immune Lysis Assay: Non-isotopic Immunoassay. Plenum Press, 389-399 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohbayashi,H.,Endo,T.,Yamashita,K.,Kuroki,M.,Matsuoka,Y.,Kobata,A.: "Novel methods to determine the epitopes on the asparagine-linked oligosaccharides of glycoproteins." Anal.Biochem.182. 200-206 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Endo,T.,Groth,D.,Prusiner,S.B.,Kobata,A.: "Diversity of oligosaccharide structures linked to asparagines of the scrapie prion protein." Biochemistry. 28. 8380-8388 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tomita,T.,Watanabe,M.,Takahashi,T.,Kumai,K.,Todakuma,T.,Yasuda,T.: "Temperature-sensitive release of adriamycin,an amphiphillic anti-tumor agent,from dipalmitoyl-phosphatidyl choline-cholesterol liposomes." Biochim.Biophys.Acta. 978. 185-190 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hosoda,K.,Yasuda,T.: "Homogeneous immunoassay for alpha-2 plasmin inhibitor and alpha-2PI-plasmin complex.Application of sandwich liposome immune lysis assay technique." J.Immunol.Methods. 121. 121-128 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Katagiri,T.,Ting,J.P.,Dy,R.,Prokop,C.,Cohen,P.,Earp,H.S.: "Tyrosine phosphorylation of a c-Src-like protein is increased in membranes of CD4^-CD8^-T lymphocytes from 1pr/1pr mice." Mol.Cell.Biol.9. 4914-4922 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Katagiri,T.,Urakawa,K.,Yamanashi,Y.,Semba,K.,Takahashi,T.,Kano,K.: "Overexpression of src family gene for tyrosine-kinase p59^<fyn> in CD4^-CD8^-T cells of mice with a lymphoproliferative disorder." Proc.Natl.Acad.Sci.86. 10064-10068 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kobata,A.,Furukawa,K.: "Handbook on glycoproteins" Marcel Dekker,Inc.New York, (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yasuda,T.,Ishimori,Y.,Umeda,M.: "Liposome Immune Lysis Assay:Non-isotopic Immunoassay" Plenum Press, 389-399 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Amano,J.;Nishimura,R.;Mochizuki,M.;Kobata,A.: J.Biol.Chem.263. 1157-1165 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kobata,A.: Gann Monograph Cancer Res.34. 3-13 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sadahira,Y.;Mori,M.;Awai,M.;Watarai,S.;Yasuda,T.: Blood. 72. 42-48 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Watarai,S.;Onuma,M.;Yasuda,T.: Jpn.J.Vet.Sci.50. 463-469 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Umeda,M.;Tomita,T.;Shibata,H.;Seki,M.;Yasuda,T.: J.Clin.Microbiology. 26. 804-807 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kobata,A.: Biochimie.

    • Related Report
      1988 Annual Research Report
  • [Publications] Yasuda,T.;Ishimori,Y.;Umeda,M.: "Immunoassay using fluorescent dye-trapped liposomes Liposome immune lysis assay(LISA)" Plenum Press, 389-399 (1988)

    • Related Report
      1988 Annual Research Report

URL: 

Published: 1988-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi