生体分子応答性ゲルを用いたマイクロアクチュエータシステムの構築

• Project Area: Development of Molecular Robots equipped with sensors and intelligence
• Project/Area Number: 15H00827
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Kansai University
• Principal Investigator: 宮田 隆志 関西大学, 化学生命工学部, 教授 (50239414)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 生体分子応答性ゲル / 分子認識 / 動的架橋 / アクチュエータ / マイクロデバイス / マイクロ流路 / 分子複合体 / 刺激応答性

Outline of Annual Research Achievements

本研究では，生体分子応答性ゲルのコンセプトを拡張し，標的分子を入力としてインプットすると動的架橋のネットワーク構造変化により情報変換し，マクロな体積変化を出力情報としてアウトプットできるスマートゲルシステムの創出を試みる。平成28年度は以下のような研究成果が得られた。
(i)屈曲する生体分子応答性ゲルの合成とマイクロアクチュエータシステムの構築：平成27年度と同様にポリアクリルアミド(PAAm)ゲル層の片面上で，リガンドとしてシクロデキストリン(CD)，鋳型分子としてビスフェノールA(BPA)を用いた分子インプリント法によりBPA応答性ゲル層を形成させた。この2層ゲルの構造を最適に設計したところ，BPA水溶液中で一方向に曲がる明確な屈曲挙動を示した。したがって，BPAに応答して屈曲する分子応答性ゲルの合成に成功した。
(ii)膨潤収縮する生体分子応答性ゲルの合成とマイクロアクチュエータシステムの構築：光学顕微鏡を用いてマイクロ流路内に応答膨潤型のグルコース応答性ゲルと応答収縮型のBPA応答性ゲルを調製した。前者のグルコース応答性ゲルは，グルコース存在下で速やかに膨潤し，その応答挙動は動的架橋として導入したレクチン－糖複合体の含有率によって変化した。一方，後者のBPA応答性ゲルは，BPAに応答して収縮した。マイクロ流路にグルコースあるいはBPAを流すと，グルコース応答性ゲルでは流量が減少し，BPA応答性ゲルでは流量が増加した。したがって，グルコース応答性ゲルは標的分子に応答して自律的にクローズするマイクロバルブ，BPA応答性ゲルはオープンするマイクロバルブとして利用できることがわかった。したがって，生体分子応答性ゲルは自律応答型マイクロ流路制御バルブとして利用できることが明らかとなった。

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Conformationally Regulated Molecular Binding and Release of Molecularly Imprinted Polypeptide Hydrogels That Undergo Helix-Coil Transition (2017)
  • Author(s): K. Matsumoto, A. Kawamura, T. Miyata
  • Journal Title: Macromolecules Volume: 50 Issue: 5 Pages: 2136-2144
  • DOI: 10.1021/acs.macromol.6b02688
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Selective Removal of Dilute Benzene from Water by Poly(methyl methacrylate)-graft-Poly(dimethylsiloxane) Membranes Containing Hydrophobic Ionic Liquid by Pervaporation (2016)
  • Author(s): T. Uragami, E. Fukuyama, T. Miyata
  • Journal Title: J. Membrane Sci Volume: 510 Pages: 131-140
  • DOI: 10.1016/j.memsci.2016.01.057
  • Peer Reviewed
• [Journal Article] QCM sensing of bisphenol A using molecularly imprinted hydrogel/conducting polymer matrix (2016)
  • Author(s): Kazuya Matsumoto, Brylee David B Tiu, Akifumi Kawamura, Rigoberto C Advincula, Takashi Miyata
  • Journal Title: Polymer Journal Volume: 48 Issue: 4 Pages: 525-532
  • DOI: 10.1038/pj.2016.23
  • NAID: 40020778477
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Permeation and Separation Characteristics in Removal of Dilute Volatile Organic Compounds from Aqueous Solutions through Copolymer Membranes Consisted of Poly(styrene) and Poly(dimethylsiloxane) Containing a Hydrophobic Ionic Lquid by Pervaporation (2016)
  • Author(s): T, Uragami, Y. Matsuoka, T. Miyata
  • Journal Title: J. Membrane Sci. Volume: 506 Pages: 109-118
  • DOI: 10.1016/j.memsci.2016.01.031
  • Peer Reviewed
• [Journal Article] Removal of Dilute Benzene in Water through Ionic Liquid/Poly(vinyl chloride) Membrnes by Pervaporation (2016)
  • Author(s): T. Uragami, Y. Matsuoka, T. Miyata
  • Journal Title: J. Membrane Sci. Res. Volume: 2 Pages: 20-25
  • Peer Reviewed
• [Journal Article] 動的架橋を利用した刺激応答性ゲルの設計と応用 (2016)
  • Author(s): 宮田隆志
  • Journal Title: 膜 Volume: 41 Pages: 226-232
  • NAID: 130005421188
  • Acknowledgement Compliant
• [Journal Article] Development of protein-recognition SPR devices by combination of SI-ATRP with biomolecular imprinting using protein ligands (2016)
  • Author(s): Rinyarat Naraprawatphong, Genta Kawanaka, Masayoshi Hayashi, Akifumi Kawamura, Takashi Miyata
  • Journal Title: Molecular Imprinting Volume: 4 Issue: 1 Pages: 21-30
  • DOI: 10.1515/molim-2016-0003
  • Peer Reviewed / Open Access
• [Journal Article] Structural transition of pH-responsive poly(L-lysine) hydrogel prepared via chemical crosslinking (2015)
  • Author(s): Kazuya Matsumoto, Akifumi Kawamura, Takashi Miyata
  • Journal Title: Chemistry Letters Volume: 44 Issue: 10 Pages: 1284-1286
  • DOI: 10.1246/cl.150464
  • NAID: 130005101473
  • Peer Reviewed
• [Presentation] 革新的膜工学のための新規な応答性膜システム材料の設計 (2017)
  • Author(s): 宮田隆志
  • Organizer: 日本化学会第97春季年会(2017) ATPセッション「革新的膜工学の研究最前線」
  • Place of Presentation: 慶應義塾大学，東京
  • Invited
• [Presentation] Rational Rational Design of Stimuli-Responsive Gels Using Dynamic Crosslinks and Their Various Applications (2017)
  • Author(s): T. Miyata
  • Organizer: 11th International Gel Symposium (GelSympo 2017)
  • Place of Presentation: Nihon University, Chiba, Japan
  • Int'l Joint Research / Invited
• [Presentation] 光反応を利用した刺激応答性ポリマーの設計 (2017)
  • Author(s): 宮田隆志
  • Organizer: 第150回ラドテック研究会講演会
  • Place of Presentation: ，第150回ラドテック研究会講演会，東京理科大学，東京
  • Invited
• [Presentation] Design of Drug-Loaded Polypeptide Hydrogels via Molecular Imprinting and Their Controlled Release by Helix-Coil Transition (2016)
  • Author(s): K. Matsumoto, Y. Ito, A. Kawamura, T. Miyata
  • Organizer: PNG2016
  • Place of Presentation: KTH Royal Institute of Technology, Stockholm, Sweden
  • Int'l Joint Research
• [Presentation] QCM Sensing of Bisphenol A Using Molecularly Imprinted Gel Layers on Electropolymerized Terthiophene Films (2016)
  • Author(s): K. Matsumoto, B. D. Tiu, A. Kawamura, R. C. Advincula, T. Miyata
  • Organizer: The 10th Conference of Aseanian Membrane Society (AMS10)
  • Place of Presentation: Nara Kasugano International Forum IRAKA, Nara, Japan
  • Int'l Joint Research
• [Presentation] Loading of Drugs within Polypeptide Hydrogels via Molecular Imprinting and Their Controlled Release (2016)
  • Author(s): K. Matsumoto, Y. Ito, A. Kawamura, T. Miyata
  • Organizer: The 11th SPSJ International Polymer Conference
  • Place of Presentation: Fukuoka International Congress Center, Fukuoka, Japan
  • Int'l Joint Research
• [Presentation] Rational Design of Stimuli-Responsive Polymeric Materials Using Photocrosslinking and Their Applications (2016)
  • Author(s): T. Miyata
  • Organizer: RadTech Asia 2016
  • Place of Presentation: Hilton Tokyo Odaiba, Tokyo, Japan
  • Int'l Joint Research / Invited
• [Presentation] Responsive Membrane Materials for Medical and Environmental Applications (2016)
  • Author(s): T. Miyata
  • Organizer: The 10th Conference of Aseanian Membrane Society (AMS10)
  • Place of Presentation: Nara Kasugano International Forum IRAKA, Nara, Japan
  • Int'l Joint Research / Invited
• [Presentation] Rational Design of Stimuli-responsive Biomaterials Using Biomolecular Complex Crosslinks (2016)
  • Author(s): T. Miyata
  • Organizer: 2016 The 2nd International Symposium on Biointerface Science and Engineering
  • Place of Presentation: National Taiwan University, Taiwan
  • Int'l Joint Research / Invited
• [Presentation] 動的構造を利用した刺激応答性ゲルの設計と応用 (2016)
  • Author(s): 宮田隆志
  • Organizer: 日本膜学会第38年会
  • Place of Presentation: 早稲田大学，東京
  • Invited
• [Presentation] 分子間相互作用に着目した刺激応答性ゲルの設計と応用 (2016)
  • Author(s): 宮田隆志
  • Organizer: 日本材料学会 第90回高分子材料セミナー
  • Place of Presentation: 滋賀県立大学，滋賀
  • Invited
• [Presentation] 生体分子を利用した刺激応答性ゲルの設計と応用 (2016)
  • Author(s): 宮田隆志
  • Organizer: 日本学術振興会産学協力研究委員会第174委員会「分子ナノテクノロジー」第54 回研究会
  • Place of Presentation: 京都テルサ，京都
  • Invited
• [Presentation] Designs of Target Molecule-responsive Gels with Cyclodextrins and Their Smart Properties (2015)
  • Author(s): T. Miyata
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2015 (PACIFIChem 2015)
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Preparation of Glucose-responsive Shape-memory Hydrogels Using Molecular Complexes as Dynamic Crosslinks (2015)
  • Author(s): M. Hayashi, A. Kawamura, T. Miyata
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2015 (PACIFIChem 2015)
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Synthesis of Dual Stimuli-responsive Polymers That Undergo Sol-Gel Phase Transition by Light and Biomolecule (2015)
  • Author(s): K. Okuma, A. Kawamura, T. Miyata
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2015 (PACIFIChem 2015)
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Design of Molecularly Imprinted Polypeptide Hydrogels for Drug Delivery (2015)
  • Author(s): K. Matsumoto, Y. Ito, A. Kawamura, T. Miyata
  • Organizer: 25th Annual Meeting of MRS-Japan 2015
  • Place of Presentation: 横浜市開港記念会館，横浜情報文化センター，万国橋会議センター，波止場会館，産業貿易センタービル，神奈川県民ホール，神奈川
  • Year and Date: 2015-12-08
  • Int'l Joint Research
• [Presentation] 動的架橋を利用した医療・環境用スマート材料の設計 (2015)
  • Author(s): 宮田隆志
  • Organizer: 第24回ポリマー材料フォーラム
  • Place of Presentation: タワーホール船堀，東京
  • Year and Date: 2015-12-02
  • Invited
• [Presentation] Designs of Stimuli-Responsive Bioconjugated Gels with Dynamic Crosslinks and Their Smart Fuctions (2015)
  • Author(s): T. Miyata
  • Organizer: 13th Japan-Belgium Symposium on Polymer Science (13J-BSPS)
  • Place of Presentation: Nara Women’s University, Japan
  • Year and Date: 2015-11-17
  • Int'l Joint Research / Invited
• [Presentation] Designs of Stimuli-Responsive Bioconjugated Gels with Dynamic Crosslinks and Their Smart Fuctions (2015)
  • Author(s): T. Miyata
  • Organizer: IUPAC 11th International Conference on Advanced Polymers via Macromolecular Engineering (APME 2015)
  • Place of Presentation: Pacifico Yokohama Conference Center, Kanagawa
  • Year and Date: 2015-10-19
  • Int'l Joint Research / Invited
• [Presentation] Loading of Drug within Molecularly Imprinted Polypeptide Hydrogels and Its Controlled Release (2015)
  • Author(s): K. Matsumoto, A. Kawamura, T. Miyata
  • Organizer: IUPAC 11th International Conference on Advanced Polymers via Macromolecular Engineering
  • Place of Presentation: Pacifico Yokohama Conference Center, Kanagawa
  • Year and Date: 2015-10-18
  • Int'l Joint Research
• [Presentation] Stimuli-Responsive Hydrogels with Biomolecular Complexes as Dynamic Crosslinks (2015)
  • Author(s): T. Miyata
  • Organizer: International Science & Nature Congress (ISNaC) 2015 -International Symposium on Materials Science and Nanotechnology (ISMSN)
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Year and Date: 2015-09-21
  • Int'l Joint Research / Invited
• [Presentation] 光架橋を利用したスマート材料の設計と応用 (2015)
  • Author(s): 宮田隆志
  • Organizer: ラドテック研究会第38回UV/EB表面加工入門講座
  • Place of Presentation: 大阪市立工業研究所，大阪
  • Year and Date: 2015-07-15
  • Invited
• [Presentation] 革新的刺激応答性ゲル：設計戦略と応用 (2015)
  • Author(s): 宮田隆志
  • Organizer: 繊維加工技術研究会第49回講演会
  • Place of Presentation: 大阪産業創造館，大阪
  • Year and Date: 2015-06-26
  • Invited
• [Presentation] Molecular Recognition and Responsive Behavior of Molecularly Imprinted Polypeptide Hydrogels That Undergo Helix-Coil Transition (2015)
  • Author(s): K. Matsumoto, A. Kawamura, T. Miyata
  • Organizer: 第64回高分子年次大会
  • Place of Presentation: 札幌コンベンションセンター，北海道
  • Year and Date: 2015-05-27
• [Book] Biomaterials Nanoarchitectonics (2016)
  • Author(s): A. Kawamura, T. Miyata
  • Total Pages: 362
  • Publisher: Elsevier
• [Book] Encyclopedia of Polymeric Nanomaterials (2016)
  • Author(s): A. Kawamura, T. Miyata
  • Total Pages: 2672
  • Publisher: Springer
• [Book] DDSキャリア作製プロトコル集 (2015)
  • Author(s): 宮田隆志
  • Total Pages: 262
  • Publisher: シーエムシー
• [Patent(Industrial Property Rights)] 正浸透圧利用システムの駆動溶液およびその再生方法 (2016)
  • Inventor(s): 宮田隆志，河村暁文，中里高典
  • Industrial Property Rights Holder: 学校法人関西大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-139271
  • Filing Date: 2016-07-04