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カロテノイドを光捕集系とするレチナールタンパク質の創出と展開

Publicly Offered Research

Project AreaChemical conversion of solar energy by artificial photosynthesis: a breakthrough by fusion of related fields toward realization of practical processes
Project/Area Number 15H00878
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionOkayama University

Principal Investigator

須藤 雄気  岡山大学, 医歯薬学総合研究科, 教授 (10452202)

Project Period (FY) 2015-06-29 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsロドプシン / カロテノイド / 光化学 / 光遺伝学 / 光生物学 / 生物物理学 / 生物物理 / エネルギー移動 / 発色団 / 分子認識 / 生体分子 / レチナール
Outline of Annual Research Achievements

ロドプシン(レチナールタンパク質)は、動物から微生物まで幅広い生物が共通にもっている光受容体タンパク質であり、アポタンパク質を構成する7本の膜貫通αヘリックスの中央部に、アルデヒド型ビタミンAである発色団レチナールが結合した構造を持つ。ロドプシンは、クロロフィルの吸収がほとんどない領域の光(450-600 nm)を吸収することで機能する。具体的には、光吸収によりレチナールの異性化が起こり、続いて様々な特徴を持った光中間体(活性型)となることで、多彩な機能(視覚、ATP合成、膜電位の調節など)が発現する。本研究では、ロドプシンの反応を生物の光応答のモデルと捉え、新規分子の探索・解析とその高機能化を実現することで、人工光合成の可能性を拡げることを目的とした研究を行い、以下の3つの成果をあげた。

[1] ロドプシンは一般に熱に不安定で、その不安定性は高機能化を行う上で支障になる。そこで、安定な分子の探索と解析を行い、安定化分子の取得に成功した。またそれらの高分解能構造を明らかにし、安定化機構の解明に成功した。
[2] 新しい機能を持ったロドプシンの発見や創成は、新しい光生物応答制御を可能とする。そこで、新規ロドプシンの単離・取得・創成を行った。その結果、二価アニオン・SO42-を輸送する新規分子の発見・解析と輸送方向・基質の変換に成功した。
[3] ロドプシンの光反応素子および光遺伝学ツールとしての有用性を高めるため、その高機能化を試みた。アミノ酸変異によるイオン輸送力の10倍の向上、カロテノイドアンテナ結合型分子の創成に成功した。
これらの成果は、JACS誌2報、Sci. Rep.誌2報を含む原著論文として報告するなど、H27-28年の公募期間2年間の間に、当初想定した予想を超える成果をあげることが出来たと自負している。

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (28 results)

All 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (16 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 11 results,  Acknowledgement Compliant: 5 results,  Open Access: 10 results) Presentation (10 results) (of which Int'l Joint Research: 1 results,  Invited: 10 results) Remarks (1 results)

  • [Int'l Joint Research] The Hebrew University/Weizman Institute of Science(イスラエル)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Demonstration of a light-driven SO42- transporter and its spectroscopic characteristics2017

    • Author(s)
      Niho A, Yoshizawa S, Tsukamoto T, Kurihara M, Tahara S, Nakajima Y, Mizuno M, Kuramochi H, Tahara T, Mizutani Y, *Sudo Y.
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 139 Issue: 12 Pages: 4376-4389

    • DOI

      10.1021/jacs.6b12139

    • NAID

      120006425837

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A phylogenetically distinctive and extremely heat stable light-driven proton pump from the eubacterium Rubrobacter xylanophilus DSM 9941T2017

    • Author(s)
      Kanehara K, Yoshizawa S, Tsukamoto T, *Sudo Y.
    • Journal Title

      Sci. Rep.

      Volume: 7 Issue: 1 Pages: 44427-44427

    • DOI

      10.1038/srep44427

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] An inhibitory role of Arg-84 in anion channelrhodopsin-2 expressed in Escherichia coli2017

    • Author(s)
      Doi S, Tsukamoto T, Yoshizawa S, & *Sudo Y.
    • Journal Title

      Sci. Rep.

      Volume: 7 Issue: 1 Pages: 41879-41879

    • DOI

      10.1038/srep41879

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Implications for the light-driven chloride ion transport mechanism of Nonlabens marinus rhodopsin 3 by its photochemical characteristics2017

    • Author(s)
      *Tsukamoto T, Yoshizawa S, Kikukawa T, Demura M, *Sudo Y.
    • Journal Title

      J. Phys. Chem. B

      Volume: 121 Issue: 9 Pages: 2027-2038

    • DOI

      10.1021/acs.jpcb.6b11101

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural and Functional Studies on Photoactive Retinal Proteins: Light Becomes Drugs with Proteins2016

    • Author(s)
      須藤雄気
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 136 Issue: 2 Pages: 185-189

    • DOI

      10.1248/yakushi.15-00229-3

    • NAID

      130005121476

    • ISSN
      0031-6903, 1347-5231
    • Year and Date
      2016-02-01
    • Related Report
      2015 Annual Research Report
  • [Journal Article] Temperature independence of ultrafast photo-isomerization in thermophilic rhodopsin: Assessment vs other microbial proton pumps2016

    • Author(s)
      Iyer ES, Misra R, Maity A, Lioubashevski O, Sudo Y, Sheves M, *Ruhman S.
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 138 Issue: 38 Pages: 12401-12407

    • DOI

      10.1021/jacs.6b05002

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Live-cell single-molecule imaging of the cytokine receptor MPL for analysis of dynamic dimerization2016

    • Author(s)
      *Sakamoto A, Tsukamoto T, Furutani Y, Sudo Y, Shimada K, Tomita A, Kiyoi H, Kato T, Funatsu T.
    • Journal Title

      J. Mol. Cell Biol.

      Volume: 8 Issue: 6 Pages: 553-555

    • DOI

      10.1093/jmcb/mjw027

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] X-ray crystallographic structure of thermophilic rhodopsin: implications for high thermal stability and optogenetic function2016

    • Author(s)
      Tsukamoto T, Mizutani K, Hasegawa T, Takahashi M, Honda N, Hashimoto N, Shimono K, Yamashita K, Yamamoto M, Miyauchi S, Takagi S, Hayashi S, Murata T, Sudo Y.
    • Journal Title

      J. Biol. Chem.

      Volume: in press Issue: 23 Pages: 12223-12232

    • DOI

      10.1074/jbc.m116.719815

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Functional and Photochemical Characterization of a Light-Driven Proton Pump from the Gammaproteobacterium Pantoea vagans.2016

    • Author(s)
      Sudo Y, Yoshizawa S.
    • Journal Title

      Photochem. Photobiol.

      Volume: in press Issue: 3 Pages: 420-427

    • DOI

      10.1111/php.12585

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] オプトジェネティクス2016

    • Author(s)
      須藤雄気
    • Journal Title

      光と生命の事典

      Volume: 1 Pages: 372-373

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Factors affecting the stability and performance of ionic liquid-based planar transient photodetectors2015

    • Author(s)
      *Dalgleish, S., Reissig, L., Hu, L., Matsushita, M.M., Sudo, Y., & *Awaga, K.
    • Journal Title

      Langmuir

      Volume: 印刷中 Issue: 18 Pages: 5235-5243

    • DOI

      10.1021/la504972q

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Atomistic design of microbial opsin-based blue-shifted optogenetics tools2015

    • Author(s)
      Kato HE, Kamiya M, Sugo S, Ito J, Taniguchi R, Orito A, Hirata K, Inutsuka A,Yamanaka A, Maturana AD, Ishitani R, Sudo Y, Hayashi S, Nureki O
    • Journal Title

      Nat Commun

      Volume: 6 Issue: 1 Pages: 7177-7177

    • DOI

      10.1038/ncomms8177

    • NAID

      120005745621

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structural and functional roles of the N- and C-terminal extended modules in channelrhodopsin-12015

    • Author(s)
      Doi S, Mori A, Tsukamoto T, Reissig L, Ihara K, Sudo Y.
    • Journal Title

      Photochem. Photobiol. Sci.

      Volume: 14 Issue: 9 Pages: 1628-1636

    • DOI

      10.1039/c5pp00213c

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Retinal Proteins in Thermophilic Bacteria2015

    • Author(s)
      塚本卓、須藤雄気
    • Journal Title

      Seibutsu Butsuri

      Volume: 55 Issue: 2 Pages: 092-094

    • DOI

      10.2142/biophys.55.092

    • NAID

      130005061232

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2015 Annual Research Report
    • Open Access
  • [Journal Article] Color tuning in retinylidene proteins2015

    • Author(s)
      Katayama K, Sekharan S, Sudo, Y.
    • Journal Title

      Optogenetics: Light-Sensing Proteins and Their Applications

      Volume: Chapter 7 Pages: 89-107

    • DOI

      10.1007/978-4-431-55516-2_7

    • ISBN
      9784431555155, 9784431555162
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Journal Article] Microbial rhodopsins: wide distribution, rich diversity and great potential2015

    • Author(s)
      Kurihara M, Sudo Y.
    • Journal Title

      Biophysics and Physicobiology

      Volume: 12 Issue: 0 Pages: 121-129

    • DOI

      10.2142/biophysico.12.0_121

    • NAID

      130005131092

    • ISSN
      2189-4779
    • Related Report
      2015 Annual Research Report
    • Open Access
  • [Presentation] レチナールタンパク質を基盤とした光遺伝学ツール開発2017

    • Author(s)
      須藤雄気
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      宮城県・仙台市
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 光受容レチナールタンパク質の 分子機能から考える オプトジェネティクス研究の10年後2016

    • Author(s)
      須藤雄気
    • Organizer
      2015年度 第2回バイオ単分子研究会
    • Place of Presentation
      いわき市
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Towards production of retinal protein-based optogenetic tools2016

    • Author(s)
      須藤雄気
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      茨城県・つくば市
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Comparative study of the ion flux pathway in stator units of proton- and sodium-driven flagellar motors2016

    • Author(s)
      須藤雄気
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      茨城県・つくば市
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 発色団に着目したロドプシンの機能変換による光操作ツールの開発2016

    • Author(s)
      須藤雄気
    • Organizer
      第8回光操作研究会
    • Place of Presentation
      東京都・港区
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 温泉微生物が持つ光受容レチナールタンパク質の機能・構造解析2016

    • Author(s)
      須藤雄気、塚本卓
    • Organizer
      第19回日本光生物学協会年会
    • Place of Presentation
      東京都・文京区
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 山あり谷ありの光受容ロドプシンタンパク質研究2016

    • Author(s)
      須藤雄気
    • Organizer
      金沢大学バイオAFM先端研究センターセミナー
    • Place of Presentation
      石川県・金沢市
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] A rich diversity of microbial photoactive retinal proteins with a wide variety of biological functions2015

    • Author(s)
      Sudo Y.
    • Organizer
      The 7th Asia and Oceania Conference for Photobiology (AOCP)
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2015-11-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 微生物光受容レチナールタンパク質の多様性と可能性を探る2015

    • Author(s)
      須藤雄気
    • Organizer
      光化学討論会 光生物関係シンポジウム, 光・生物学:光を感じ、光で操る
    • Place of Presentation
      大阪市
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 光を薬へ!?:レチナールタンパク質を「知る」「変える」「役立てる」2015

    • Author(s)
      須藤雄気
    • Organizer
      第55回 生物物理学会若手の会 夏の学校
    • Place of Presentation
      琵琶湖
    • Year and Date
      2015-08-23
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Remarks] 研究室ホームページ

    • URL

      http://www.pharm.okayama-u.ac.jp/lab/bukka/index.html

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report

URL: 

Published: 2015-07-02   Modified: 2023-03-16  

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