病原体の侵入を細胞壁が感知する機構および意義

• Project Area: Plant cell wall as information-processing system
• Project/Area Number: 15H01247
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: 中神 弘史 国立研究開発法人理化学研究所, 環境資源科学研究センター, ユニットリーダー (20435663)
• Project Period (FY): 2015-06-29 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 植物免疫 / 細胞壁 / リン酸化プロテオミクス / DAMP

Outline of Annual Research Achievements

植物は、病原体由来の成分を直接的に認識する免疫システムに加えて、細胞壁を利用して病原体の侵入を間接的に感知するシステムを備えている。これまでに植物の細胞壁多糖類であるオリゴガラクツロン酸（OG; oligogalacturonic acid）が、膜受容体であるWAK（wall-associated kinase）を介して自身によって認識され、DAMP（damage- associated molecular pattern）として機能していることが見出されてきた。植物の細胞壁には、オリゴガラクツロン酸以外に多様な多糖類があり、それらもDAMPとして機能する可能性が考えられる。そこで、オリゴガラクツロン酸以外の細胞壁多糖類がDAMP活性を有するかを、活性酸素種（ROS）生成の誘導活性を指標にして解析を行った。その結果、オリゴガラクツロン酸以外で、シロイヌナズナまたはゼニゴケに対してDAMP活性を有する多糖類があることを見出すことに成功した。活性を示した細胞壁多糖類について、活性本体を調べるための更なる解析を進めている。

また、リン酸化プロテオームの変動解析により同定した、オリゴガラクツロン酸刺激によりリン酸化制御を受ける因子の幾つかについて、T-DNA挿入変異体を単離し、形質転換体を作出して、詳細な機能解析を進めた。その結果、幾つかの新奇因子については、病害抵抗性に関与していることが分かった。

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Dampening of the Immune Response by Changes in PUB22 Ubiquitination Modes Triggered by MPK3 (2017)
  • Author(s): Furlan G, Nakagami H, Eschen-Lippold L, Jiang X, Majovsky P, Kowarschik K, Hoehenwarter W, Lee J, Trujillo M
  • Journal Title: The Plant Cell Volume: Epub ahead of print Issue: 4 Pages: 726-745
  • DOI: 10.1105/tpc.16.00654
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Regulation of sugar transporter activity for antibacterial defense in Arabidopsis (2016)
  • Author(s): Yamada K, Saijo Y, Nakagami H, Takano Y
  • Journal Title: Science Volume: 354(6318) Issue: 6318 Pages: 1427-1430
  • DOI: 10.1126/science.aah5692
  • NAID: 120005895801
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Modulation of plant growth in vivo and identification of kinase substrates using an analog-sensitive variant of CYCLIN-DEPENDENT KINASE A;1 (2016)
  • Author(s): Harashima H, Dissmeyer N, Hammann P, Nomura Y, Kramer K, Nakagami H, Schnittger A
  • Journal Title: BMC Plant Biology Volume: 16(1):209 Issue: 1
  • DOI: 10.1186/s12870-016-0900-7
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Circadian profiling of the Arabidopsis proteome using 2D-DIGE (2016)
  • Author(s): Choudhary MK, Nomura Y, Shi H, Nakagami H, Somers DE
  • Journal Title: Frontiers in Plant Science Volume: 7:1007
  • DOI: 10.3389/fpls.2016.01007
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The Arabidopsis CERK1-associated kinase PBL27 connects chitin perception to MAPK activation (2016)
  • Author(s): Yamada K, Yamaguchi K, Shirakawa T, Nakagami H, Mine A, Ishikawa K, Fujiwara M, Narusaka M, Narusaka Y, Ichimura K, Kobayashi Y, Matsui H, Nomura Y, Nomoto M, Tada Y, Fukao Y, Fukamizo T, Tsuda K, Shirasu K, Shibuya N, Kawasaki T
  • Journal Title: The EMBO Journal Volume: 35(22) Issue: 22 Pages: 2468-2483
  • DOI: 10.15252/embj.201694248
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] TDM1 regulation determines the number of meiotic divisions (2016)
  • Author(s): Cifuentes M, Jolivet S, Cromer L, Harashima H, Bulankova P, Renne C, Crismani W, Nomura, Nakagami H, Sugimoto K, Schnittger A, Riha K, Mercier R
  • Journal Title: PLOS Genetics Volume: 12(2) Issue: 2 Pages: e1005856-e1005856
  • DOI: 10.1371/journal.pgen.1005856
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Chitin nanofiber elucidates the elicitor activity of polymeric chitin in plants (2015)
  • Author(s): Egusa, M., Matsui, H., Urakami, T., Okuda, S., Ifuku, S., Nakagami, H. and Kaminaka, H.
  • Journal Title: frontiers in Plant Science Volume: 6 Pages: 1098-1098
  • DOI: 10.3389/fpls.2015.01098
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] HR cell death regulator “MARK1” is associated with P-bodies in Arabidopsis (2017)
  • Author(s): Hidenori Matsui, Yuko Nomura, Takahiro Hamada, Gang-Su Hyon, Yuichiro Watanabe, Takashi Ueda, Hirofumi Nakagami
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学・鹿児島市
  • Year and Date: 2017-03-16
• [Presentation] Characterization of components involved in immune system in Marchantia polymorpha (2017)
  • Author(s): Izumi Yotsui, Hidekazu Iwakawa, Hidenori Matsui, Yuko Nomura, Katharina Kramer, Anne Harzen, Tatsuhiko Kanazawa, Ryuichi Nishihama, Shinpei Katou, Takashi Ueda, Takayuki Kochi, Hirofumi Nakagami
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学・鹿児島市
  • Year and Date: 2017-03-16
• [Presentation] Izumi Yotsui, Hidenori Matsui, Gang-Su Hyon, Yuko Nomura, Ryuichi Nishihama, Takayuki Kohchi, Hirofumi Nakagami (2016)
  • Author(s): Marchantia as a new tool to understand plant immune system
  • Organizer: Cold Spring Harbor Asia
  • Place of Presentation: 淡路夢舞台国際会議場・淡路市
  • Year and Date: 2016-12-01
  • Int'l Joint Research
• [Presentation] Evolutionary proteomics to understand plant immune system (2016)
  • Author(s): Hirofumi Nakagami, Izumi Yotsui, Hidenori Matsui, Gang-Su Hyon, Yuko Nomura, Ryuichi Nishihama, Takayuki Kohchi
  • Organizer: 8th Asia Oceania Human Proteome Organization Congress
  • Place of Presentation: Sun Moon Lake, Taiwan
  • Year and Date: 2016-09-23
  • Int'l Joint Research / Invited
• [Presentation] 庭の嫌われ者「ゼニゴケ」に学ぶ植物免疫システム (2016)
  • Author(s): 中神弘史
  • Organizer: 平成28 年度植物感染生理談話会
  • Place of Presentation: 神戸市須磨浦海岸シーパル須磨・神戸市
  • Year and Date: 2016-08-12
  • Invited
• [Presentation] 進化プロテオミクスによる植物免疫システムの理解 (2016)
  • Author(s): 四井いずみ、松井英譲、玄康洙、野村有子、西浜竜一、河内孝之、中神弘史
  • Organizer: 日本プロテオーム学会2016年大会
  • Place of Presentation: 北里大学薬学部 白金キャンパス・東京都
  • Year and Date: 2016-07-28
  • Invited
• [Presentation] PAMP-responsive phosphoprotein MARK1 limits program cell death induction (2016)
  • Author(s): Hidenori Matsui, Yuko Nomura, Mayumi Egusa, Gang-Su Hyon, Hironori Kaminaka, Marco Trujillo, Ken Shirasu, Hirofumi Nakagami
  • Organizer: 2016 IS-MPMI XVII Congress
  • Place of Presentation: Portland, USA
  • Year and Date: 2016-07-17
  • Int'l Joint Research
• [Presentation] Elucidation of basic framework of plant immune system using Marchantia (2016)
  • Author(s): Hirofumi Nakagami, Izumi Yotsui, Hidenori Matsui, Yuko Nomura, Ryuichi Nishihama, Takayuki Kohchi
  • Organizer: EMBO Workshop
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2016-06-23
  • Int'l Joint Research / Invited
• [Presentation] MAMP応答性リン酸化タンパク質MARK1は病原菌感染による細胞死を制御する (2016)
  • Author(s): 松井英譲、野村有子、玄康洙、江草真由美、上中弘典、中神弘史
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター・岡山市
  • Year and Date: 2016-03-23
• [Presentation] MAMP応答性プロテインキナーゼMRPK1のリン酸化部位変異体の解析 (2016)
  • Author(s): 玄康洙、松井英譲、野村有子、中神弘史
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター・岡山市
  • Year and Date: 2016-03-22
• [Presentation] Phospho-regulation of MARK1 and MARK32 (2016)
  • Author(s): Hidenori Matsui, Gang-Su Hyon, Yuko Nomura, Hirofumi Nakagami
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学・盛岡市
  • Year and Date: 2016-03-19
• [Presentation] Chitin recognition system in the basal land plant Marchantia polymorpha (2016)
  • Author(s): Izumi Yotsui, Hidenori Matsui, Yuko Nomura, Ryuichi Nishihama, Takayuki Kohchi, Hirofumi Nakagami
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学・盛岡市
  • Year and Date: 2016-03-19
• [Presentation] When proteomics meets Marchantia -Highroad to understand plant immune signaling- (2016)
  • Author(s): Hirofumi Nakagami
  • Organizer: Special symposium at the 1st international Marchantia training course
  • Place of Presentation: 京都大学・京都市
  • Year and Date: 2016-03-03
  • Int'l Joint Research / Invited
• [Presentation] Towards durable and broad-spectrum resistance: Post-genomic strategies to understand conserved immune system in land plants (2015)
  • Author(s): Hidenori Matsui, Izumi Yotsui, Gang-Su Hyon, Yuko Nomura, Hirofumi Nakagami
  • Organizer: Tsukuba Global Science Week 2015
  • Place of Presentation: つくば国際会議場・つくば市
  • Year and Date: 2015-09-28
  • Int'l Joint Research / Invited