発達期神経回路再編成の定量コネクトミクス解析

• Project Area: Mechanisms underlying the functional shift of brain neural circuitry for behavioral adaptation
• Project/Area Number: 15H01413
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University
• Principal Investigator: 八尾 寛 東北大学, 生命科学研究科, 教授 (00144353)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2016: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000); Fiscal Year 2015: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
• Keywords: コネクトミクス / 軸索分枝 / 軸索刈込み / カスパーゼ / ロドプシン / 光遺伝学 / トランスジェニックラット / Cre-loxPシステム / 神経科学 / 脳・神経 / 生理学 / 遺伝子 / 発生・分化 / シナプス / 軸索 / 発達 / 臨界期 / 競合 / 数理モデル / ニワトリ胚

Outline of Annual Research Achievements

前年度の研究を発展させ、軸索刈込みにカスパーゼが機能しているという仮説を検証した。カスパーゼ３のドミナント・ネガティブ変異体遺伝子(C3DN)を中脳Edinger-Westphal核ニューロンに導入し、軸索において過剰発現した時の軸索分枝パターンをコントロールと比較した。その結果、6日胚(E6)のC3DN群において、分枝が減少し、分枝長が増大していることを見出した。すなわち、標的投射過程の軸索においては、分子数の増加と分枝長のホメオスターシスがあり、分子数の増大と分枝長の減少で特徴づけられるpathfinding modeと、分子数の減少と分枝長の増大をともなうtargeting modeの動的平衡状態にあること、およびカスパーゼ３の活性化により、targeting modeに傾くことが示唆された(Katow et al., Dev. Growth Diff, 2017, in press)。原核生物由来のNa+ポンプ型ロドプシンは、光遺伝学分子ツールとして、Cl-やH+などのイオン環境の影響を受けないとともに影響しないメリットがある(Kato et al., Nature, 2015)。しかし、膜発現効率が低い欠点があった。本研究においては、２種類のNa+ポンプ型ロドプシンをもとに、膜発現効率において改善された光遺伝学分子ツールを作製した(Hoque et al., PLoS ONE, 2016)。Cre-loxPシステムによりコンディショナルに明るい赤色蛍光タンパク質のtdTomatoを発現するレポーターラットを報告した(Igarashi et al., PLoS ONE, 2016)。In vivoニューロンにおいては、tdTomatoの極めて明るい蛍光が得られ、突起の形態や軸索の投射の解析に最適化されていた。以上の研究により、本新学術領域研究の促進に貢献した。

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Johns Hopkins University/National University of Singapore(シンガポール)
• [Journal Article] In Vivo Spiking Dynamics of Intra- and Extratelencephalic Projection Neurons in Rat Motor Cortex. (2017)
  • Author(s): Saiki A, Sakai Y, Fukabori R, Soma S, Yoshida J, Kawabata M, Yawo H, Kobayashi K, Kimura M, Isomura Y.
  • Journal Title: Cereb Cortex. Volume: 印刷中 Issue: 3 Pages: 1-15
  • DOI: 10.1093/cercor/bhx012
  • Peer Reviewed
• [Journal Article] Kinetic characteristics of chimeric channelrhodopsins implicate the molecular identity involved in desensitization (2017)
  • Author(s): Alemeh Zamani, Shigeo Sakuragi, Toru Ishizuka, Hiromu Yawo.
  • Journal Title: Biophysics and Physicobiology Volume: 14 Issue: 0 Pages: 13-22
  • DOI: 10.2142/biophysico.14.0_13
  • NAID: 130005303959
  • ISSN: 2189-4779
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Regulation of axon arborization pattern in the developing chick ciliary ganglion: Possible involvement of caspase 3. (2017)
  • Author(s): Katow, Hidetaka; Kanaya, Teppei; Ogawa, Tomohisa; Egawa, Ryo; Yawo, Hiromu.
  • Journal Title: Development Growth and Differentiation Volume: 59 Issue: 3 Pages: 115-128
  • DOI: 10.1111/dgd.12346
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The regulatory mechanism of ion permeation through a channelrhodopsin derived from Mesostigma viride (MvChR1) (2016)
  • Author(s): Watanabe S, Ishizuka T, Hososhima S, Zamani A, Hoque MR, Yawo H
  • Journal Title: Photochemical & Photobiological Sciences Volume: 15 Issue: 3 Pages: 365-374
  • DOI: 10.1039/c5pp00290g
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A Novel Reporter Rat Strain That Conditionally Expresses the Bright Red Fluorescent Protein tdTomato. (2016)
  • Author(s): Igarashi H, Koizumi K, Kaneko R, Ikeda K, Egawa R, Yanagawa Y, Muramatsu S, Onimaru H, Ishizuka T, Yawo H.
  • Journal Title: PLoS One. Volume: 11 Issue: 5 Pages: e0155687-e0155687
  • DOI: 10.1371/journal.pone.0155687
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Position- and quantity-dependent responses in zebrafish turning behavior. (2016)
  • Author(s): Umeda K, Ishizuka T, Yawo H, Shoji W.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 27888-27888
  • DOI: 10.1038/srep27888
  • Peer Reviewed / Open Access
• [Journal Article] Integration of Organic Electrochemical and Field-Effect Transistors for Ultraflexible, High Temporal Resolution Electrophysiology Arrays. (2016)
  • Author(s): Lee W, Kim D, Rivnay J, Matsuhisa N, Lonjaret T, Yokota T, Yawo H, Sekino M, Malliaras GG, Someya T.
  • Journal Title: Adv Mater. Volume: 28 Issue: 44 Pages: 9722-9728
  • DOI: 10.1002/adma.201602237
  • Peer Reviewed
• [Journal Article] Adrenergic receptor-mediated modulation of striatal firing patterns. (2016)
  • Author(s): Ohta H, Kohno Y, Arake M, Tamura R, Yukawa S, Sato Y, Morimoto Y, Nishida Y, Yawo H.
  • Journal Title: Neurosci Res. Volume: 112 Pages: 47-56
  • DOI: 10.1016/j.neures.2016.05.004
  • Peer Reviewed
• [Journal Article] A Chimera Na+-Pump Rhodopsin as an Effective Optogenetic Silencer. (2016)
  • Author(s): Hoque MR, Ishizuka T, Inoue K, Abe-Yoshizumi R, Igarashi H, Mishima T, Kandori H, Yawo H.
  • Journal Title: PLoS One. Volume: 11 Issue: 11 Pages: e0166820-e0166820
  • DOI: 10.1371/journal.pone.0166820
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Blockade of the KATP channel Kir6.2 by memantine represents a novel mechanism relevant to Alzheimer's disease therapy. (2016)
  • Author(s): Moriguchi S, Ishizuka T, Yabuki Y, Shioda N, Sasaki Y, Tagashira H, Yawo H, Yeh JZ, Sakagami H, Narahashi T, Fukunaga K.
  • Journal Title: Mol Psychiatry. Volume: 印刷中 Issue: 2 Pages: 211-221
  • DOI: 10.1038/mp.2016.187
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural basis for Na+ transport mechanism by a light-driven Na+ pump (2015)
  • Author(s): H. E. Kato, K. Inoue, R. Abe-Yoshizumi, Y. Kato, H. Ono, M. Konno, S. Hososhima, T. Ishizuka, M. R. Hoque, H. Kunitomo, J. Ito, S. Yoshizawa, K. Yamashita, M. Takemoto, T. Nishizawa, R. Taniguchi, K. Kogure, A. D. Maturana, Y. Iino, H. Yawo, R. Ishitani, H. Kandori*, O. Nureki*
  • Journal Title: Nature Volume: 未定 Issue: 7550 Pages: 48-53
  • DOI: 10.1038/nature14322
  • Peer Reviewed / Open Access
• [Journal Article] Chimeras of Channelrhodopsin-1 and -2 from Chlamydomonas reinhardtii Exhibit Distinctive Light-induced Structural Changes from Channelrhodopsin-2. (2015)
  • Author(s): Asumi Inaguma, Hisao Tsukamoto, Hideaki E. Kato, Tetsunari Kimura, Toru Ishizuka, Satomi Oishi, Hiromu Yawo, Osamu Nureki, Yuji Furutani
  • Journal Title: J. Biol. Chem. Volume: 290 Issue: 18 Pages: 11623-11634
  • DOI: 10.1074/jbc.m115.642256
  • Peer Reviewed / Open Access
• [Journal Article] Loss of α1,6-fucosyltransferase decreased hippocampal long-term potentiation: implications for core fucosylation in the regulation of AMPA receptor heteromerization and cellular signaling. (2015)
  • Author(s): Gu, W., Fukuda, T., Isaji, T., Hang, Q., Lee, H., Sakai, S., Morise, J., Mitoma, J., Higashi, H., Taniguchi, N., Yawo, H., Oka, S. and Gu, J.
  • Journal Title: J. Biol. Chem. Volume: 290 Issue: 28 Pages: 17566-75
  • DOI: 10.1074/jbc.m114.579938
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Phox2b BAC Transgenic Rat Line Useful for Understanding Respiratory Rhythm Generator Neural Circuitry. (2015)
  • Author(s): Ikeda K, Takahashi M, Sato S, Igarashi H, Ishizuka T, Yawo H, Arata S, Southard-Smith EM, Kawakami K, Onimaru H.
  • Journal Title: PLoS One Volume: 10 Issue: 7 Pages: 1-23
  • DOI: 10.1371/journal.pone.0132475
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Membrane depolarization regulates intracellular RANKL transport in non-excitable osteoblasts (2015)
  • Author(s): Notomi T, Kuno M, Ezura Y, Honma M, Ishizuka T, Ohura K, Yao H and Noda M.
  • Journal Title: Bone Volume: 81 Pages: 306-314
  • DOI: 10.1016/j.bone.2015.07.031
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Near-infrared (NIR) up-conversion optogenetics. (2015)
  • Author(s): Shoko Hososhima, Hideya Yuasa, Toru Ishizuka, Mohammad R. Hoque, Takayuki Yamashita, Akihiro Yamanaka, Eriko Sugano, Hiroshi Tomita, and Hiromu Yawo
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 16533-16533
  • DOI: 10.1038/srep16533
  • NAID: 120007179836
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Engineering Biological Systems for Light (2015)
  • Author(s): 石塚徹、江川遼、梅田桂子、東海林亙、八尾寛
  • Journal Title: Seibutsu Butsuri Volume: 55 Issue: 6 Pages: 311-316
  • DOI: 10.2142/biophys.55.311
  • NAID: 130005110040
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed / Open Access
• [Journal Article] 「巻頭言」光と生命の融合 (2015)
  • Author(s): 八尾寛
  • Journal Title: 光学 Volume: 44 Pages: 415-415
• [Journal Article] オプトジェネティクス（光遺伝学）の情報革命 (2015)
  • Author(s): 八尾寛
  • Journal Title: 化学と工業 Volume: 68 Pages: 1012-1017
• [Presentation] Optical manipulation of intracellular Ca2+ dynamics in Neuron (2017)
  • Author(s): Kaichi Sugihara, Hiroyuki Igarashi, Toru Ishizuka, Hiromu Yawo
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 浜松
  • Year and Date: 2017-03-28
• [Presentation] All-optical analysis of mesoscopic circuit function in the primary somatosensory cortex of mice (2017)
  • Author(s): Kyo Koizumi, Hiromu Yawo
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 浜松
  • Year and Date: 2017-03-28
• [Presentation] Upconversion-rhodopsin hybrid system for near-infrared manipulation of neural network (2017)
  • Author(s): Hiromu Yawo, Shoko Hososhima, Hideya Yuasa, Takayuki Yamashita, Akihiro Yamanaka, Toru Ishizuka
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 浜松
  • Year and Date: 2017-03-28
• [Presentation] Organelle-optogenetics -Intervention of the intracellular Ca2+ dynamics by light- (2017)
  • Author(s): Hiroyuki Igarashi, Ayaka Yoshikawa, Toshifumi Asano, Ayumu Konno, Toru Ishizuka, Hiromu Yawo
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 浜松
  • Year and Date: 2017-03-28
  • Invited
• [Presentation] Is the pre-post ratio of motor projection dependent on the number of competitors? - a quantitative connectomics study (2017)
  • Author(s): Takahiro Takahashi, Hiromu Yawo
  • Organizer: The 2017 Japan-NIH joint Symposium
  • Place of Presentation: 仙台
  • Year and Date: 2017-02-15
  • Int'l Joint Research
• [Presentation] 新規トランスジェニックラット3 系統の開発- tdTomato レポーターラット, Flame ラット, ChRFR(C167A)レポーターラット (2017)
  • Author(s): 五十嵐敬幸, 小泉協, 金子涼輔, 池田啓子, 江川遼, 柳川右千夫, 村松慎一, 鬼丸洋, 石塚徹, 八尾寛
  • Organizer: 先端モデル動物支援プラットフォーム平成28年度成果発表会
  • Place of Presentation: 大津
  • Year and Date: 2017-02-06
• [Presentation] Functional mapping of rat barrel cortex for spatiotemporal optogenetic patterns to whiskers (2017)
  • Author(s): 劉越人, 櫻木繁雄, 八尾寛
  • Organizer: 先端モデル動物支援プラットフォーム平成28年度成果発表会
  • Place of Presentation: 大津
  • Year and Date: 2017-02-06
• [Presentation] Receptive field of a neuron in the rat barrel cortex - optogenetic whisker stimulation with a spatiotemporal pattern (2017)
  • Author(s): Hiromu Yawo
  • Organizer: the Nanyang Technological University (NTU) workshop on “Shedding light on the brain: merging neuroscience and photonics”
  • Place of Presentation: Singapore, Singapore
  • Int'l Joint Research / Invited
• [Presentation] 発達期神経回路再編成の定量コネクトミクス解析 (2016)
  • Author(s): 江川 遼
  • Organizer: 第４回「適応回路シフト」領域班会議
  • Place of Presentation: 東京
  • Year and Date: 2016-12-19
• [Presentation] 小胞体Ca2+ダイナミクスの光操作－適応回路シフト研究の新展開 (2016)
  • Author(s): 五十嵐 敬幸
  • Organizer: 第４回「適応回路シフト」領域班会議
  • Place of Presentation: 東京
  • Year and Date: 2016-12-19
• [Presentation] オプトジェネティクス革命 (2016)
  • Author(s): 八尾寛
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば
  • Year and Date: 2016-11-25
  • Invited
• [Presentation] Spectral characteristics of chimeric channelrhodopsins implicate the molecular identity involved in desensitization (2016)
  • Author(s): Alemeh Zamani, Toru Ishizuka, Hiromu Yawo
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば
  • Year and Date: 2016-11-25
• [Presentation] 大脳皮質局所回路のin vivoオール光機能解析 (2016)
  • Author(s): 小泉協, 八尾寛
  • Organizer: 生理学研究所研究会「シナプス伝達の細胞分子調節機構」
  • Place of Presentation: 岡崎
  • Year and Date: 2016-11-21
• [Presentation] Upconversion-rhodopsin hybrid system for near-infared optogenetics (2016)
  • Author(s): Shoko Hososhima, Hideya Yuasa, Toru Ishizuka, Hiromu Yawo
  • Organizer: 17th International Conference on Retinal Proteins
  • Place of Presentation: Potsdam, Germany
  • Year and Date: 2016-10-02
  • Int'l Joint Research
• [Presentation] Intervention of the intracellular Ca2+ dynamics by light - light-sensitive myoblasts as in vitro model of sarcomere assembly - (2016)
  • Author(s): Hiroyuki Igarashi, Ayaka Yoshikawa, Toshifumi Asano, Ayumu Konno, Toru Ishizuka, Hiromu Yawo
  • Organizer: 17th International Conference on Retinal Proteins
  • Place of Presentation: Potsdam, Germany
  • Year and Date: 2016-10-02
  • Int'l Joint Research
• [Presentation] 小胞体Ca2+ダイナミクスの光操作 - 光感受性C2C12を用いたSarcomere assembly 研究モデル (2016)
  • Author(s): 五十嵐敬幸, 吉川彩香, 浅野豪文, 石塚徹, 八尾寛
  • Organizer: 第2回日本筋学会学術集会
  • Place of Presentation: 小平市
  • Year and Date: 2016-08-05
• [Presentation] オルガネラ光操作 (2016)
  • Author(s): 八尾寛
  • Organizer: ルミノジェネティクス研究会
  • Place of Presentation: 熱海
  • Year and Date: 2016-07-23
  • Invited
• [Presentation] オール光のin vivoメゾスコピック脳機能解析 (2016)
  • Author(s): 八尾寛
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
  • Invited
• [Presentation] Up-conversion optogenetic system using near-infrared (NIR) light (2016)
  • Author(s): Hiromu Yawo, Shoko Hososhima, Mohammad Razuanul Hoque, Hideya Yuasa, Takayuki Yamashita, Akihiro Yamanaka, Toru Ishizuka
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] Optogenetic conditioning of paradigms discrimination in the rat whisker-barreL system (2016)
  • Author(s): Kenta Abe, Hiromu Yawo
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] Involvement of caspase system in the regulation of axon arborization during early developmental stages of chick ciliary ganglion (2016)
  • Author(s): Hidetaka Katow, Toru Ishizuka, Hiromu Yawo
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] All-optical approach to study mesoscopic circuitry in the primary somatosensory cortex of mouse (2016)
  • Author(s): Kyo Koizumi,. Masaaki Sato, Junichi Nakai, Masamichi Ohkura, Yasunori Hayashi, Hiromu Yawo
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] Optogenetic silencing of neural activity using a chimeric light-driven Na+-transporter rhodopsin (2016)
  • Author(s): Mohammad Hoque, Toru Ishizuka, Hiromu Yawo
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] Functional mapping of rat barrel cortex for spatiotemporal optogenetic patterns to whisker (2016)
  • Author(s): Yueren Liu, Tomokazu Ohshiro, Hiromu Yawo
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] Optogenetics therapy for restoring locomotion after severe spinal cord injury (2016)
  • Author(s): Kenta Takashima, Naoji Matsuhisa, Akira Shimada, Peter Zalar, Tomoyuki Yokota, Masaki Sekino, Hiromu Yawo, Takao Someya, Hiroshi Onodera
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] Takayuki Yamashita, Hideya Yuasa, Hiromu Yawo, Akihiro Yamanaka (2016)
  • Author(s): Takayuki Yamashita, Hideya Yuasa, Hiromu Yawo, Akihiro Yamanaka
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] How do axons scramble for targets? - Activity-dependent axonal reorganization in developing chick ciliary ganglion (2016)
  • Author(s): Ryo Egawa, Hiromu Yawo
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] 発達期神経回路再編成の定量コネクトミクス解析 (2016)
  • Author(s): 八尾 寛
  • Organizer: 第３回「適応回路シフト」領域班会議
  • Place of Presentation: 猪苗代町
  • Year and Date: 2016-06-30
• [Presentation] 軸索はどのようにして標的を奪い合うのか？：発達期軸索投射再編における神経活動の役割 (2016)
  • Author(s): 江川 遼
  • Organizer: 第３回「適応回路シフト」領域班会議
  • Place of Presentation: 猪苗代町
  • Year and Date: 2016-06-30
• [Presentation] Near-infrared (NIR) controlled optogenetics in zebrafish larva (2016)
  • Author(s): Keiko Umeda, Shoko Hososhima, Toru Ishizuka, Hiromu Yawo, Wataru Shoji
  • Organizer: 発生生物学会第49回大会
  • Place of Presentation: 熊本
  • Year and Date: 2016-05-31
• [Presentation] ウィスカ―バレル野の感覚入力における時空間パターン弁別 (2016)
  • Author(s): 阿部健太、八尾 寛
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2016-03-22
• [Presentation] All-optical approach to study signal integration in the somatosensory cortex of mouse (2016)
  • Author(s): Koizumi, K., Sato, M., Ohkura, M., Nakai, J., Hayashi, Y., Yawo, H.
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2016-03-22
• [Presentation] Near-infared (NIR) up-conversion optogenetics for neural manipulation (2016)
  • Author(s): Shoko Hososhima, Hideya Yuasa, Eriko Sugano, Hiroshi Tomita, Toru Ishizuka, Hiromu Yawo
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2016-03-22
• [Presentation] Involvement of caspase system in the regulation of axon arborization during early developmental stages of chick ciliary ganglion (2016)
  • Author(s): H. Katow, T. Ishizuka, and H. Yawo
  • Organizer: Dissecting and Understanding Mechanisms for Functional Shift of Brain Network
  • Place of Presentation: 同志社大学（京都府・京都市）
  • Year and Date: 2016-03-03
  • Int'l Joint Research
• [Presentation] オプトジェネティクス（光遺伝学）～光による生命機能制御～ (2016)
  • Author(s): 八尾 寛
  • Organizer: 分子ナノテクノロジー第174委員会 第53回研究会
  • Place of Presentation: 京都大学東京品川オフィス（東京都・港区）
  • Year and Date: 2016-03-02
  • Invited
• [Presentation] 光と生命の融合 (2016)
  • Author(s): 八尾寛
  • Organizer: 最先端計測とライフサイエンスの近未来
  • Place of Presentation: 仙台
  • Invited
• [Presentation] 神経細胞における小胞体Ca2+ダイナミクスの光操作 (2016)
  • Author(s): 杉原嘉一, 五十嵐敬幸, 石塚徹, 八尾寛
  • Organizer: 第48回東北生理談話会
  • Place of Presentation: 盛岡
• [Presentation] Near-infrared (NIR) light control of neural activities using up-conversion of lanthanide nanoparticles (2015)
  • Author(s): Shoko Hososhima, Hideya Yuasa, Toru Ishizuka, Mohammad Razuanul Hoque, Hiromu Yawo
  • Organizer: 第7回光操作研究会国際シンポジウム
  • Place of Presentation: 東京医科歯科大学（東京都・文京区）
  • Year and Date: 2015-12-04
  • Int'l Joint Research
• [Presentation] Novel transgenic animals for tracing and optogenetics -Flame rats and bistable ChR reporter rats- (2015)
  • Author(s): H. IGARASHI, K. KOIZUMI, R. KANEKO, K. NISHIZAWA, Y. YANAGAWA, S.-I. MURAMATSU, K. KOBAYASHI, T. ISHIZUKA, H. YAWO
  • Organizer: 第7回光操作研究会国際シンポジウム
  • Place of Presentation: 東京医科歯科大学（東京都・文京区）
  • Year and Date: 2015-12-04
  • Int'l Joint Research
• [Presentation] ランタニドナノ粒子アップコンバージョンを利用した近赤外光操作 (2015)
  • Author(s): 八尾寛
  • Organizer: 理研研究会「シナプス・神経ネットワークの機能ダイナミクス」
  • Place of Presentation: 生理学研究所（愛知県・岡崎市）
  • Year and Date: 2015-12-02
• [Presentation] OPTOGENETIC 51LENCING OF NEURONAL ACTIVITY US1NG A LlGHT-DRIVEN SODIUM ION PUMP IN MARINE BACTERIA (2015)
  • Author(s): Toru Ishizuka，Shoko Hososhima, Mohammad Razuanul Hoque, Kazuho Yoshida，Keiichi Inoue，Hideki Kandori，Hiromu Yawo
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting
  • Place of Presentation: 東北大学（宮城県・仙台市）
  • Year and Date: 2015-11-25
  • Int'l Joint Research
• [Presentation] NEAR-INFRARED (NIR) OPTOGENETICS USING UP-CONVERSION OF LANTHANIDE NANOPARTICLES (2015)
  • Author(s): Shoko Hososhima, Hideya Yuasa, Toru Ishizuka，Hiromu Yawo
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting
  • Place of Presentation: 東北大学（宮城県・仙台市）
  • Year and Date: 2015-11-25
  • Int'l Joint Research
• [Presentation] Optogenetic induction of contractile ability in C2C12 myotubes (2015)
  • Author(s): Toshifumi Asano, Toru Ishizuka, Hiromu Yawo
  • Organizer: 8th FAOPS Congress
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] Discrimination of optogenetic whisker-barrel inputs using channelrhodopsin-2 transgenic rat (2015)
  • Author(s): Kenta Abe, Hiromu Yawo
  • Organizer: 8th FAOPS Congress
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] Opt-fMRI imaging of somatosensory cortical activity using transgenic rat which expresses channelrhodopsin-2 in the peripheral mechanoreceptive neurons (2015)
  • Author(s): Yukinobu Yokoyama, Akira Sumiyoshi, Ryuta Kawashima, Hiromu Yawo
  • Organizer: 8th FAOPS Congress
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] Novel transgenic animals for tracing and optogenetics -Flame rats and bistable ChR reporter rats- (2015)
  • Author(s): H. IGARASHI, K. KOIZUMI, R. KANEKO, K. NISHIZAWA, Y. YANAGAWA, S.-I. MURAMATSU, K. KOBAYASHI, T. ISHIZUKA, H. YAWO
  • Organizer: Neuroscience 2015
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] "Multi-Linc" - an optogenetic/electrophysiological method to reveal information flows among multiple brain areas (2015)
  • Author(s): A. SAIKI, Y. SAKAI, R. FUKABORI, S. SOMA, J. YOSHIDA, H. YAWO, K. KOBAYASHI, M. KIMURA, Y. ISOMURA
  • Organizer: Neuroscience 2015
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] 光を用いて神経活動に介入する「オプトジェネティクス」 (2015)
  • Author(s): 八尾寛
  • Organizer: FAN2015 第25回インテリジェント・システム・シンポジウム
  • Place of Presentation: 東北大学（宮城県・仙台市）
  • Year and Date: 2015-09-24
  • Invited
• [Presentation] Optogenetic patterning of neural activity (2015)
  • Author(s): 八尾寛
  • Organizer: 2015年光化学討論会
  • Place of Presentation: 大阪市立大学（大阪府・大阪市）
  • Year and Date: 2015-09-09
  • Invited
• [Presentation] How do axons scramble for targets? - Quantitative connectomics of axonal reorganization during development (2015)
  • Author(s): Ryo Egawa, Toru lshizuka, Hiromu Yawo
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県・神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Near-infrared (NIR) optogenetics using up-conversion of lanthanide nanoparticles (2015)
  • Author(s): Hiromu Yawo, Shoko Hososhima, Kenta Abe, Hideya Yuasa, Eriko Sugano, Hiroshi Tomita, Toru lshizuka
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県・神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Optogenetic silencing of neural activity using a light-driven Na+ pump from marine bacteria (2015)
  • Author(s): Mohammad Razuanul Hoque, Shoko Hososhima, Kazuho Yoshida, Toru lshizuka, Keiichi lnoue, Hideki Kandori, Hiromu Yawo
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県・神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Discrimination of optogenetic whisker-barrel patterns in transgenic rats expressing channelrhodopusin-2 (2015)
  • Author(s): Kenta Abe, Toru lshizuka, Hiromu Yawo
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県・神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Flame rat - evaluation of a reporter transgenic rat line which conditionally expresses bright red fluorescent protein (tdTomato) (2015)
  • Author(s): Hiroyuki lgarashi, Kyo Koizumi, Ryosuke Kaneko, Keiko lkeda, Hiroshi Onimaru, Yuchio Yanagawa, Shin-lchi Muramatsu, Toru lshizuka, Keiichi lnoue, Hideki Kandori, Hiromu Yawo
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県・神戸市）
  • Year and Date: 2015-07-28
• [Presentation] "Multi-Linc" - a novel optogenetic/electrophysiological method to explore information flows among multiple brain areas (2015)
  • Author(s): Akiko Saiki, Yutaka Sakai, Ryoji Fukabori, Junichi Yoshida, Hiromu Yawo, Kazuto Kobayashi, Minoru Kimura, Yoshikazu lsomura
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県・神戸市）
  • Year and Date: 2015-07-28
• [Presentation] How do axons scramble for targets? - Quantitative connectomics of axonal reorganization during development (2015)
  • Author(s): 江川遼、石塚徹、八尾寛
  • Organizer: Frontiers of Brain Science: Tools & Technologies
  • Place of Presentation: 東北大学（宮城県・仙台市）
  • Year and Date: 2015-07-21
• [Presentation] Competition or coexistence? Emergence of uniform arborization pattern during developmental axon reorganization (2015)
  • Author(s): Hiromu Yawo, Satoki Sakai, Ryo Egawa
  • Organizer: Frontiers of Brain Science: Tools & Technologies
  • Place of Presentation: 東北大学（宮城県・仙台市）
  • Year and Date: 2015-07-21
• [Presentation] 神経損傷後の一次求心性神経Aβ線維を介する疼痛関連行動：光遺伝学的アプローチを用いた神経障害性疼痛の新しい評価法 (2015)
  • Author(s): 田島諒一（Ryoichi Tashima），古賀啓祐（Keisuke Koga），山本将大（Shota Yamamoto），樋口瞳（Hitomi Higuchi），高露雄太（Yuta Kohro），八尾寛（Hiromu Yawo），井上和秀（Kazuhide Inoue），古江秀昌（Hidemasa Furue），津田誠（Makoto Tsuda）
  • Organizer: 日本疼痛学会
  • Place of Presentation: 市民会館崇城大学ホール（熊本県・熊本市）
  • Year and Date: 2015-07-03
• [Presentation] ランタニドナノ粒子アップコンバージョン効果による 近赤外オプトジェネティクス (2015)
  • Author(s): 八尾寛、細島頌子、阿部健太、湯浅英哉、 菅野江里子、冨田浩史、石塚徹
  • Organizer: ナノ学会第13回大会
  • Place of Presentation: 東北大学（宮城県・仙台市）
  • Year and Date: 2015-05-11
• [Presentation] チャネルロドプシン光電変換の特性と応用 (2015)
  • Author(s): 八尾寛
  • Organizer: 理研シンポジウム「細胞システムの動態と論理 Ⅶ」
  • Place of Presentation: 理化学研究所（埼玉県・和光市）
  • Year and Date: 2015-04-02
  • Invited
• [Book] Optogenetics: Light-Sensing Proteins and Their Applications (2015)
  • Author(s): Yawo H, Kandori H, Koizumi A
  • Publisher: Springer
• [Book] Chaptor 8. General description: Future prospects of optogenetics. “Optogenetics: Light-Sensing Proteins and Their Applications” (2015)
  • Author(s): Yawo, H., Egawa, R., Hososhima, S., Wen, L.
  • Total Pages: 409
  • Publisher: Springer
• [Book] Chaptor 21. Strategies to probe mechanoreception: from mechanical to optogenetic approaches. “Optogenetics: Light-Sensing Proteins and Their Applications” (2015)
  • Author(s): Ji, Z.-G., Ishizuka, T., Yawo, H.
  • Total Pages: 409
  • Publisher: Springer
• [Remarks] 細胞/臓器/個体を自在に光らせる！世界初の赤色蛍光レポーターラット
  • URL: http://www.tohoku.ac.jp/japanese/2016/05/press20160520-02.html
• [Remarks] 反射運動に可変性をもたらす神経回路の新たな発見
  • URL: http://www.tohoku.ac.jp/japanese/2016/06/press20160613-02.html
• [Remarks] 東北大学大学院生命科学研究科
  • URL: http://www.lifesci.tohoku.ac.jp/teacher/t_yawo/
• [Remarks] 東北大学脳科学センター
  • URL: http://www.bsc.tohoku.ac.jp/researchers/yao_h/
• [Remarks] 脳機能解析分野
  • URL: http://neuro.med.tohoku.ac.jp/japanese/noukinou.html
• [Remarks] 光で筋肉を再生！ ～ALSなどの難病治療に対する新たな技術として期待～
  • URL: http://www.tohoku.ac.jp/japanese/2015/02/press20150205-01.html
• [Remarks] レアメタル・ナノ粒子の光で脳細胞を活性化-近赤外光信号によるオン・オフ制御の実現
  • URL: http://www.tohoku.ac.jp/japanese/2015/11/press20151109-01.html