• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

高速AFM計測によるKaiタンパク質のロバストな概日周期発生機構の解明

Publicly

Project AreaDynamical ordering of biomolecular systems for creation of integrated functions
Project/Area Number 16H00758
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionNagoya University (2017)
Kanazawa University (2016)

Principal Investigator

内橋 貴之  名古屋大学, 理学研究科, 教授 (30326300)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords時計タンパク質 / 高速原子間力顕微鏡 / プロテアソーム / 一分子計測 / ダイナミクス / 一分子計測(SMD) / 走査プローブ顕微鏡 / ナノバイオ / 1分子計測(SMD) / 蛋白質
Outline of Annual Research Achievements

Kaiタンパク質の動的相互作用: KaiCのリン酸化状態に依存してKaiAとの相互作用が概日周期的に変動することを見出した(Phase Dependent Differential Affinity: PDDAと名付けた )。KaiCのリン酸化概日周期について、実験で得られたパラメーターを用いて数理シミュレイションを行い、PDDAが概日周期にどのような影響を及ぼすのかを調べた。PDDAが無い場合には、KaiAとKaiCの濃度比が変動すると概日周期が消失するのに対して、PDDAがある場合には概日周期が維持される濃度比が3倍程度大きくなった。このことから、PDDAは細胞内でのタンパク質濃度の揺らぎに対するKaiシステムの頑強性に寄与していることが明らかになった。また、温度制御下でKaiA-KaiCの相互作用を調べたところ、25-29℃の温度範囲では動的親和性に大きな変化は見られえず、30℃以上では、KaiAとKaiCの親和性が大きく変化することがわかった。
プロテアソームα7ホモ14量体のα6による2ステップ解体過程:領域内共同研究としてプロテアソーム構成タンパク質α7ホモ14量体がα6により解体される過程を観察した。α7-14量体をアミノシランで化学修飾したマイカに強固に吸着させると14量体が自発的に7量体に分離する様子が見られた。さらに,α7-7量体リングの中心孔にα6サブユニットが結合・解離を繰り返し、時間経過とともにα6が中心孔に強固に結合することが分かった。また、積層した7量体リング間に隙間が経時的に生じ、そこにα6が結合する様子が観察された。これらのことから、α7-14量体のα6サブユニットによる解体は、リング積層間隙へのα6の結合と解離、7量体リング中心孔へのα6の強固な結合によるダブルリングの再生阻止の2段階の過程を経ていることを明らかにした。

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2017 Annual Research Report
  • 2016 Annual Research Report

Research Products

(63 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research Journal Article Presentation Book Patent(Industrial Property Rights)

  • [Int'l Joint Research] ヴァンダービルド大学(アメリカ合衆国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Vanderbilt University(アメリカ合衆国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Structural properties determining low K+ affinity of the selectivity filter in the TWIK1 K+ channel.2019

    • Author(s)
      Tsukamoto Hisao、Higashi Masahiro、Motoki Hideyoshi、Watanabe Hiroki、Ganser Christian、Nakajo Koichi、Kubo Yoshihiro、Uchihashi Takayuki、Furutani Yuji
    • Journal Title

      J. Biol. Chem.

      Volume: 293 Pages: 6969-6984

    • DOI
      10.1074/jbc.ra118.001817
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Applications of high-speed atomic force microscopy to real-time visualization of dynamic biomolecular processes2018

    • Author(s)
      Uchihashi Takayuki、Scheuring Simon
    • Journal Title

      BBA General Subjects

      Volume: 1862 Pages: 229-240

    • DOI
      10.1016/j.bbagen.2017.07.010
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechano-Sensitive Rate constants, processivity, and productive binding ratio of chitinase A revealed by single-molecule analysis. Ion Channels.2018

    • Author(s)
      Nakamura, A., Tasaki, T., Okuni, Y., Song, C., Murata, K., Kozai, T., Hara, M., Sugimoto, H., Suzuki, K., Watanabe, T., Uchihashi, T., Noji, H., & Iino, R.
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: 20 Pages: 010-3018

    • DOI
      10.1039/c7cp04606e
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Conversion of functionally undefined homopentameric protein PbaA into a proteasome activator by mutational modification of its C-terminal segment conformation2018

    • Author(s)
      M. Yagi-Utsumi, A. Sikdar, T. Kozai, R. Inoue, M. Sugiyama, T. Uchihashi, H. Yagi, T. Satoh, and K. Kato
    • Journal Title

      Protein Eng. Des. Sel.

      Volume: 31 Pages: 29-36

    • DOI
      10.1093/protein/gzx066
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dynamic clustering of dynamin-amphiphysin helices regulates membrane constriction and fission coupled with GTP hydrolysis.2018

    • Author(s)
      Tetsuya Takeda, Toshiya Kozai, Huiran Yang, Daiki Ishikuro, Kaho Seyama, Yusuke Kumagai, Tadashi Abe, Hiroshi Yamada, Takayuki Uchihashi, Toshio Ando, Kohji Takei
    • Journal Title

      elife

      Volume: 7 Pages: 1-19

    • DOI
      10.7554/elife.30246
    • NAID
      120006510224
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Negatively charged lipids are essential for functional and structural switch of human 2-Cys peroxiredoxin II2018

    • Author(s)
      Haruyama Takamitsu、Uchihashi Takayuki、Yamada Yutaro、Kodera Noriyuki、Ando Toshio、Konno Hiroki
    • Journal Title

      J. Mol. Biol.

      Volume: 430 Pages: 602-610

    • DOI
      10.1016/j.jmb.2017.12.020
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sweeping of adsorbed therapeutic proteins on prefillable syringe enhances subvisible particles generation2018

    • Author(s)
      Takahiro Maruno, Hiroki Watanabe, Takayuki Uchihashi, Satoru Adachi, Kunihito Arai, Taichi Sawaguchi and Susumu Uchiyama
    • Journal Title

      Journal of Pharmaceutical Sciences

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insight into structural remodeling of the FlhA ring responsible for bacterial flagellar type III protein export2018

    • Author(s)
      Naoya Terahara, Yumi Inoue, Noriyuki Kodera, Yusuke V. Morimoto, Takayuki Uchihashi, Katsumi Imada, Toshio Ando, Keiichi Namba, and Thoru Minamino
    • Journal Title

      Science Advances

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Translating MOF chemistry into supramolecular chemistry: soluble coordination nanofibers showing efficient photon upconversion2018

    • Author(s)
      Masanori Hosoyamada, Nobuhiro Yanai, Keisuke Okumura, Takayuki Uchihashi and Nobuo Kimizuka
    • Journal Title

      ChemComm

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantum-dot antibody conjugation visualized at the single-molecule scale with high-speed atomic force microscopy2018

    • Author(s)
      Takayuki Umakoshi, Hikari Udaka, Takayuki Uchihashi, Toshio Ando, Miho Suzuki, Takeshi Fukuda
    • Journal Title

      Colloids and Surfaces B: Biointerfaces

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of Protein Dynamics using High-Speed Atomic Force Microscopy and Image Analysis2018

    • Author(s)
      内橋 貴之
    • Journal Title

      J. Comput. Chem. Jpn.

      Volume: 17 Issue: 1 Pages: 20-30

    • DOI
      10.2477/jccj.2018-0001
    • NAID
      130006528631
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM2017

    • Author(s)
      Keya Jakia Jannat、Inoue Daisuke、Suzuki Yuki、Kozai Toshiya、Ishikuro Daiki、Kodera Noriyuki、Uchihashi Takayuki、Kabir Arif Md. Rashedul、Endo Masayuki、Sada Kazuki、Kakugo Akira
    • Journal Title

      Scientific Reports

      Volume: 7 Pages: 6166-6166

    • DOI
      10.1038/s41598-017-06249-1
    • NAID
      120006368158
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fast Adsorption of Soft Hydrogel Microspheres on Solid Surfaces in Aqueous Solution2017

    • Author(s)
      Matsui Shusuke、Kureha Takuma、Hiroshige Seina、Shibata Mikihiro、Uchihashi Takayuki、Suzuki Daisuke
    • Journal Title

      Angew. Chem. Int. Ed.

      Volume: 56 Pages: 12146-12149

    • DOI
      10.1002/anie.201705808
    • NAID
      120006377474
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis2017

    • Author(s)
      Harada Hirofumi、Onoda Akira、Uchihashi Takayuki、Watanabe Hiroki、Sunagawa Naoki、Samejima Masahiro、Igarashi Kiyohiko、Hayashi Takashi
    • Journal Title

      Chemical Science

      Volume: 8 Pages: 6561-6565

    • DOI
      10.1039/c7sc01672g
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualisation of a flexible modular structure of the ER folding-sensor enzyme UGGT2017

    • Author(s)
      Satoh Tadashi、Song Chihong、Zhu Tong、Toshimori Takayasu、Murata Kazuyoshi、Hayashi Yugo、Kamikubo Hironari、Uchihashi Takayuki、Kato Koichi
    • Journal Title

      Sci. Rep.

      Volume: 7 Pages: 12142-12142

    • DOI
      10.1038/s41598-017-12283-w
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy2017

    • Author(s)
      Shibata Mikihiro、Nishimasu Hiroshi、Kodera Noriyuki、Hirano Seiichi、Ando Toshio、Uchihashi Takayuki、Nureki Osamu
    • Journal Title

      Nat. Commun.

      Volume: 8 Pages: 1430-1430

    • DOI
      10.1038/s41467-017-01466-8
    • NAID
      120006374160
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High-speed atomic force microscopy imaging of live mammalian cells2017

    • Author(s)
      Mikihiro Shibata, Hiroki Watanabe, Takayuki Uchihashi, Toshio Ando, and Ryohei Yasuda
    • Journal Title

      BIOPHYSICS

      Volume: 14 Issue: 0 Pages: 127-135

    • DOI
      10.2142/biophysico.14.0_127
    • NAID
      130005997921
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Na+-induced structural transition of MotPS for stator assembly of Bacillus flagellar motor.2017

    • Author(s)
      Terahara, N., Kodera, N., Uchihashi, T., Ando, T., Namba, K. & Minamino, T.
    • Journal Title

      Science Advances

      Volume: 3 Pages: 4119-4119

    • DOI
      10.1126/sciadv.aao4119
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oriented Reconstitution of the Full-Length KcsA Potassium Channel in a Lipid Bilayer for AFM Imaging.2017

    • Author(s)
      Ayumi Sumino, Takayuki Uchihashi and Shigetoshi Oiki
    • Journal Title

      The Journal of Physical Chemistry Letters

      Volume: 8 Pages: 785-793

    • DOI
      10.1021/acs.jpclett.6b03058
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A natural light-driven inward proton pump.2016

    • Author(s)
      Keiichi Inoue, Shota Ito, Yoshitaka Kato, Yurika Nomura, Mikihiro Shibata, Takayuki Uchihashi, Satoshi P. Tsunoda and Hideki Kandori
    • Journal Title

      Nature Communication

      Volume: 7 Pages: 13415-13415

    • DOI
      10.1038/ncomms13415
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Direct observation of self-assembly process of biological and artificial fibrils using high-speed atomic force microscopy2018

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      Interhierarchical understanding of materials and life through molecular observation
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生命の構成部品を直接みて理解する ~ 顕微鏡技術で可視化するタンパク質のダイナミクス現象 ~2018

    • Author(s)
      内橋貴之
    • Organizer
      自然科学研究機構 機構長プレス懇談会 「生きているとは何か?」~みる・よむ・つくる研究領域~
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Structural Flexibility and Chaperone Activity of TClpB revealed by High-Speed AFM2017

    • Author(s)
      Takayuki Uchihashi, Yo-hei Watanabe, Ryota Iino and Toshio Ando
    • Organizer
      XIX. Annual Linz Winter Workshop
    • Place of Presentation
      Sommerhotel Julius-Raab-Heim, Linz, Austria
    • Year and Date
      2017-02-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Direct visualization of dynamic molecular interactions using HS-AFM2017

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      Frontier Bioorganization Forum 2017: Dynamical ordering and integrated functions of biomolecular systems
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Direct visualization of single molecule dynamics by high-speed atomic force microscopy2017

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      Telluride Science Research Center Workshop on Protein Dynamics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Oligomeric state and conformational dynamics of eubacterial ion-pumping rhodopsin studied by high-speed AFM2017

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      KAKENHI International Symposium on “Studying the Function of Soft Molecular Systems”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High-speed atomic force microscopy for a tool to visualize dynamic events on biological systems from single molecules to living cells2017

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      Workshop on “Nanofluidics in Biological Systems”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Direct observation of single molecule dynamics at work with high-speed atomic force microscopy2017

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      Frontier in Single Molecule Biophysics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Visualization of Single-Molecule Dynamics Using High-Speed Atomic Force Microscopy2017

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      The 2nd Korea-Japan Joint Symposium on Single-Molecule Biophysics 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High-speed atomic force microscopy: A tool for visualizing dynamic behavior from proteins to cells2017

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      The 28th 2017 International Symposium on Micro-NanoMechanical and Human Science
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] In-line Force Measurements with High-speed AFM2017

    • Author(s)
      Christian Ganser, Takayuki Uchihashi
    • Organizer
      The 25th International Colloquium on Scanning Probe Microscopy
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Two-step process for disassembly mechanism of proteasome α7 homo-tetradecamer by α6 revealed by high-speed atomic force microscopy2017

    • Author(s)
      Toshiya Kozai, Taichiro Sekiguchi, Tadashi Satoh, Hirokazu Yagi, Koichi Kato and Takayuki Uchihashi
    • Organizer
      The 25th International Colloquium on Scanning Probe Microscopy
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of new mirro-tilter unit for tip-scanning high-speed atomic force microscopy2017

    • Author(s)
      Hiroki Watanabe, Takayuki Uchihashi and Shinnosuke Yamanaka and Toshio Ando
    • Organizer
      The 25th International Colloquium on Scanning Probe Microscopy
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dynamic Observationb of Kai Proteins by HS-AFM Reveales a Mechanism of the Robustness in the Cyanobacterial Circadian Oscillator2017

    • Author(s)
      Shogo Sugiyama, Tetsuya Mori, Mark Byne, Takayuki Uchihashi, Carl H. Johnson and Toshio Ando
    • Organizer
      The 62nd Annual Meeting of Biophysical Society
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 高速AFMで明らかにするKaiタンパク質間の動的相互作用2017

    • Author(s)
      内橋貴之
    • Organizer
      第69回 日本細胞生物学会大会, シンポジウム「分子の集合・離脱がつかさどる動的な細胞機能」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 高速原子間力顕微鏡で可視化するタンパク質の動的秩序2017

    • Author(s)
      内橋貴之
    • Organizer
      第17回 日本蛋白質科学会年会, ワークショップ「蛋白質動的秩序のマルチプローブを用いた統合的解析
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 高速AFMを用いた生体分子のその場観察2017

    • Author(s)
      内橋貴之
    • Organizer
      2017年真空・表面科学合同講演会, 合同シンポジウム「バイオ表面・界面,細胞,生体組織のオペランド計測
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 高速原子間力顕微鏡で可視化する生体・人工高分子の動態2017

    • Author(s)
      内橋貴之
    • Organizer
      2017年真空・表面科学合同講演会, 表面:プローブ顕微鏡研究部会「走査プローブ顕微鏡によるナノ表面科学の最前線」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 高速原子間力顕微鏡による生体分子のダイナミクス計測2017

    • Author(s)
      内橋貴之
    • Organizer
      新世代研究所 バイオ単分子研究会「タンパク質の作動原理の理解へ向けて - 機能する姿を活写する -
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Analysis of Rotational Dynamics of Rotorless Enterococcus hirae V1-ATPase using High-Speed Atomic Force Microscopy2017

    • Author(s)
      Motonori Imamura, Kazuya Nakamoto, Shintaro Maruyama, Fumihiro Kawai, Ryota Iino, Takayuki Uchihashi, Takeshi Murata, Toshio Ando
    • Organizer
      日本生物物理学会第55回年会, シンポジウム "Analysis of Protein Dynamics in Solution"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dynamic remodeling of Dynamin complexes during membrane fission2017

    • Author(s)
      Tetsuya Takeda, Daiki Ishikuro, Huiran Yang, Toshiya Kozai, Kaho Seyama, Yusuke Kumagai, Hiroshi Yamada, Takayuki Uchihashi, Toshio Ando, Kohji Takei
    • Organizer
      日本生物物理学会第55回年会, シンポジウム "Biophysics of molecular assembly and biological membrane"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High-Speed AFM Observation of Domain Flexibility Related to Enzymatic Function of CRISPR-Cas92017

    • Author(s)
      Takayuki Uchihashi, Mikihiro Shibata, Hiroshi Nishimasu, Noriyuki Kodera, Seiichi Hirano, Toshio Ando, Osamu Nureki
    • Organizer
      第54回日本生物物理学会年会 シンポジウム: "Softness and functions of biological molecules under various environments"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高速原子間力顕微鏡で可視化する生体膜反応ダイナミクス2017

    • Author(s)
      内橋貴之
    • Organizer
      2017年度 生命科学系合同年次大会 ワークショップ「最先端の表面科学手法による生体膜反応の実動作下計測」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 高速原子間力顕微鏡で可視化する Kai タンパク質間相互作用のダイナミクス2017

    • Author(s)
      内橋貴之
    • Organizer
      第24回日本時間生物学会学術大会 シンポジウム「24時間の創出原理」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Visualization of protein molecules in action by high-speed atomic force microscopy2016

    • Author(s)
      Mikihiro Shibata, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando
    • Organizer
      日本生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Direct Visualization of Single Molecule Dynamics at Work with High-Speed Atomic Force Microscopy2016

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      BioNANO 2016
    • Place of Presentation
      Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland
    • Year and Date
      2016-11-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] タンパク質のダイナミクスを可視化する高速原子間力顕微鏡2016

    • Author(s)
      内橋貴之
    • Organizer
      生体ボリュームイメージング研究部会&生理研研究会合同ワークショップ「電子顕微鏡ビッグデータが拓くバイオメディカルサイエンス」~限界を超えるための顕微鏡技術~
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 高速原子間力顕微鏡で可視化する生体分子のダイナミクス2016

    • Author(s)
      内橋貴之
    • Organizer
      理研シンポジウム: 第4回「光量子工学研究 -若手・中堅研究者から見た光量子工学の展開-」
    • Place of Presentation
      理化学研究所
    • Year and Date
      2016-11-01
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 高速AFMによる生体分子ダイナミクスのその場観察2016

    • Author(s)
      内橋貴之
    • Organizer
      日本物理学会2016年秋季大会 領域9シンポジウム「表面界面ナノ構造のその場観察」
    • Place of Presentation
      金沢大学角間キャンパス
    • Year and Date
      2016-09-15
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 高速AFMによる生体試料の動態イメージング2016

    • Author(s)
      内橋貴之
    • Organizer
      第25回日本バイオイメージング学会学術集会 シンポジウム3「ナノバイオイメージング:1分子から細胞までの先端手法」
    • Place of Presentation
      名古屋市立大学
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Dynamic interaction between Kai proteins dependent on phosphorylation states of KaiC revealed by HS-AFM2016

    • Author(s)
      Takayuki Uchihashi, Tetsuya Mori, Shogo Sugiyama, Carl H. Johnson, and Toshio Ando
    • Organizer
      4th Kanazawa Bio-AFM Workshop 2016
    • Place of Presentation
      KKRホテル金沢
    • Year and Date
      2016-09-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Direct Visualization of Single Molecule Dynamics by High-Speed Atomic Force Microscopy2016

    • Author(s)
      Takayuki Uchihashi, Noriyuki Kodera and Toshio Ando
    • Organizer
      Gordon Research Conference, Single Molecule Approach to Biology
    • Place of Presentation
      Chinese University of Hong Kong, Hong Kong
    • Year and Date
      2016-07-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Visualization of Functional Dynamics of Biological Molecules by High-Speed AFM2016

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      分子研研究会 “超機能分子の創成 合成、計測、数理が織りなす社会実装分子の戦略的設計と開発”
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2016-06-27
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 高速原子間力顕微鏡で調べる回転分子モーターの構造ダイナミクス2016

    • Author(s)
      内橋貴之
    • Organizer
      第16回 日本蛋白質科学会年会 ワークショップ”回転分子モーターを調べてつくって理解する”
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2016-06-09
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 高速AFMによる膜タンパク質のダイナミクス観察2016

    • Author(s)
      内橋貴之
    • Organizer
      蛋白研セミナー ”膜タンパク質の構造ダイナミクス”
    • Place of Presentation
      大阪大学蛋白質科学研究所
    • Year and Date
      2016-05-12
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Direct Observation of Single Molecule Dynamics at Work with High-Speed Atomic Force Microscopy2016

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      Les Houches-TSRC Workshop on Protein Dynamics
    • Place of Presentation
      Ecole de Physique des Houches, France
    • Year and Date
      2016-04-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] "High-Speed Atomic Force Microcopy in "Compendium of Surface and Interface Analysis2018

    • Author(s)
      Takayuki Uchihashi
    • Publisher
      Springer
    • Related Report
      2017 Annual Research Report
  • [Book] パリティ、「高速原子間力顕微鏡によるタンパク質の動画撮影」2018

    • Author(s)
      内橋貴之
    • Publisher
      丸善出版
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 走査型プローブ顕微鏡2016

    • Inventor(s)
      内橋貴之,柴田幹大, 古寺哲幸
    • Industrial Property Rights Holder
      内橋貴之,柴田幹大, 古寺哲幸
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-234584
    • Filing Date
      2016-12-01
    • Application Year
      2016
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 昇温ホルダおよびプローブ顕微鏡2016

    • Inventor(s)
      内橋貴之,足立彗, 古寺哲幸
    • Industrial Property Rights Holder
      内橋貴之,足立彗, 古寺哲幸
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-233494
    • Filing Date
      2016-11-30
    • Application Year
      2016
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] チャンバーアレイの製造方法2016

    • Inventor(s)
      古寺哲幸,豊田貴大,内橋貴之
    • Industrial Property Rights Holder
      古寺哲幸,豊田貴大,内橋貴之
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-232100
    • Filing Date
      2016-11-30
    • Application Year
      2016
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-26   Modified: 2018-12-17  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi