高速AFM計測によるKaiタンパク質のロバストな概日周期発生機構の解明

• Project Area: Dynamical ordering of biomolecular systems for creation of integrated functions
• Project/Area Number: 16H00758
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Nagoya University (2017); Kanazawa University (2016)
• Principal Investigator: 内橋 貴之 名古屋大学, 理学研究科, 教授 (30326300)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 時計タンパク質 / 高速原子間力顕微鏡 / プロテアソーム / 一分子計測 / ダイナミクス / 一分子計測(SMD) / 走査プローブ顕微鏡 / ナノバイオ / １分子計測(SMD) / 蛋白質

Outline of Annual Research Achievements

Kaiタンパク質の動的相互作用： KaiCのリン酸化状態に依存してKaiAとの相互作用が概日周期的に変動することを見出した(Phase Dependent Differential Affinity: PDDAと名付けた )。KaiCのリン酸化概日周期について、実験で得られたパラメーターを用いて数理シミュレイションを行い、PDDAが概日周期にどのような影響を及ぼすのかを調べた。PDDAが無い場合には、KaiAとKaiCの濃度比が変動すると概日周期が消失するのに対して、PDDAがある場合には概日周期が維持される濃度比が3倍程度大きくなった。このことから、PDDAは細胞内でのタンパク質濃度の揺らぎに対するKaiシステムの頑強性に寄与していることが明らかになった。また、温度制御下でKaiA-KaiCの相互作用を調べたところ、25-29℃の温度範囲では動的親和性に大きな変化は見られえず、30℃以上では、KaiAとKaiCの親和性が大きく変化することがわかった。
プロテアソームα7ホモ14量体のα６による2ステップ解体過程：領域内共同研究としてプロテアソーム構成タンパク質α7ホモ14量体がα6により解体される過程を観察した。α7-14量体をアミノシランで化学修飾したマイカに強固に吸着させると14量体が自発的に7量体に分離する様子が見られた。さらに，α7-7量体リングの中心孔にα6サブユニットが結合・解離を繰り返し、時間経過とともにα6が中心孔に強固に結合することが分かった。また、積層した７量体リング間に隙間が経時的に生じ、そこにα6が結合する様子が観察された。これらのことから、α7-14量体のα6サブユニットによる解体は、リング積層間隙へのα6の結合と解離、７量体リング中心孔へのα6の強固な結合によるダブルリングの再生阻止の２段階の過程を経ていることを明らかにした。

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] ヴァンダービルド大学(アメリカ合衆国)
• [Int'l Joint Research] Vanderbilt University(アメリカ合衆国)
• [Journal Article] Structural properties determining low K+ affinity of the selectivity filter in the TWIK1 K+ channel. (2019)
  • Author(s): Tsukamoto Hisao、Higashi Masahiro、Motoki Hideyoshi、Watanabe Hiroki、Ganser Christian、Nakajo Koichi、Kubo Yoshihiro、Uchihashi Takayuki、Furutani Yuji
  • Journal Title: J. Biol. Chem. Volume: 293 Pages: 6969-6984
  • DOI: 10.1074/jbc.ra118.001817
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Applications of high-speed atomic force microscopy to real-time visualization of dynamic biomolecular processes (2018)
  • Author(s): Uchihashi Takayuki、Scheuring Simon
  • Journal Title: BBA General Subjects Volume: 1862 Pages: 229-240
  • DOI: 10.1016/j.bbagen.2017.07.010
  • Peer Reviewed
• [Journal Article] Mechano-Sensitive Rate constants, processivity, and productive binding ratio of chitinase A revealed by single-molecule analysis. Ion Channels. (2018)
  • Author(s): Nakamura, A., Tasaki, T., Okuni, Y., Song, C., Murata, K., Kozai, T., Hara, M., Sugimoto, H., Suzuki, K., Watanabe, T., Uchihashi, T., Noji, H., & Iino, R.
  • Journal Title: Phys. Chem. Chem. Phys. Volume: 20 Pages: 010-3018
  • DOI: 10.1039/c7cp04606e
  • Peer Reviewed / Open Access
• [Journal Article] Conversion of functionally undefined homopentameric protein PbaA into a proteasome activator by mutational modification of its C-terminal segment conformation (2018)
  • Author(s): M. Yagi-Utsumi, A. Sikdar, T. Kozai, R. Inoue, M. Sugiyama, T. Uchihashi, H. Yagi, T. Satoh, and K. Kato
  • Journal Title: Protein engineering design & selection Volume: 31 Pages: 29-36
  • DOI: 10.1093/protein/gzx066
  • Peer Reviewed / Open Access
• [Journal Article] Dynamic clustering of dynamin-amphiphysin helices regulates membrane constriction and fission coupled with GTP hydrolysis. (2018)
  • Author(s): Tetsuya Takeda, Toshiya Kozai, Huiran Yang, Daiki Ishikuro, Kaho Seyama, Yusuke Kumagai, Tadashi Abe, Hiroshi Yamada, Takayuki Uchihashi, Toshio Ando, Kohji Takei
  • Journal Title: elife Volume: 7 Pages: 1-19
  • DOI: 10.7554/elife.30246
  • Peer Reviewed / Open Access
• [Journal Article] Negatively charged lipids are essential for functional and structural switch of human 2-Cys peroxiredoxin II (2018)
  • Author(s): Haruyama Takamitsu、Uchihashi Takayuki、Yamada Yutaro、Kodera Noriyuki、Ando Toshio、Konno Hiroki
  • Journal Title: J. Mol. Biol. Volume: 430 Pages: 602-610
  • DOI: 10.1016/j.jmb.2017.12.020
  • Peer Reviewed / Open Access
• [Journal Article] Sweeping of adsorbed therapeutic proteins on prefillable syringe enhances subvisible particles generation (2018)
  • Author(s): Takahiro Maruno, Hiroki Watanabe, Takayuki Uchihashi, Satoru Adachi, Kunihito Arai, Taichi Sawaguchi and Susumu Uchiyama
  • Journal Title: Journal of Pharmaceutical Sciences Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Insight into structural remodeling of the FlhA ring responsible for bacterial flagellar type III protein export (2018)
  • Author(s): Naoya Terahara, Yumi Inoue, Noriyuki Kodera, Yusuke V. Morimoto, Takayuki Uchihashi, Katsumi Imada, Toshio Ando, Keiichi Namba, and Thoru Minamino
  • Journal Title: Science Advances Volume: 印刷中
  • Peer Reviewed / Open Access
• [Journal Article] Translating MOF chemistry into supramolecular chemistry: soluble coordination nanofibers showing efficient photon upconversion (2018)
  • Author(s): Masanori Hosoyamada, Nobuhiro Yanai, Keisuke Okumura, Takayuki Uchihashi and Nobuo Kimizuka
  • Journal Title: ChemComm Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Quantum-dot antibody conjugation visualized at the single-molecule scale with high-speed atomic force microscopy (2018)
  • Author(s): Takayuki Umakoshi, Hikari Udaka, Takayuki Uchihashi, Toshio Ando, Miho Suzuki, Takeshi Fukuda
  • Journal Title: Colloids and Surfaces B: Biointerfaces Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Visualization of Protein Dynamics using High-Speed Atomic Force Microscopy and Image Analysis (2018)
  • Author(s): 内橋 貴之
  • Journal Title: Journal of Computer Chemistry, Japan Volume: 17 Issue: 1 Pages: 20-30
  • DOI: 10.2477/jccj.2018-0001
  • Peer Reviewed
• [Journal Article] High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM (2017)
  • Author(s): Keya Jakia Jannat、Inoue Daisuke、Suzuki Yuki、Kozai Toshiya、Ishikuro Daiki、Kodera Noriyuki、Uchihashi Takayuki、Kabir Arif Md. Rashedul、Endo Masayuki、Sada Kazuki、Kakugo Akira
  • Journal Title: Scientific Reports Volume: 7 Pages: 6166-6166
  • DOI: 10.1038/s41598-017-06249-1
  • Peer Reviewed / Open Access
• [Journal Article] Fast Adsorption of Soft Hydrogel Microspheres on Solid Surfaces in Aqueous Solution (2017)
  • Author(s): Matsui Shusuke、Kureha Takuma、Hiroshige Seina、Shibata Mikihiro、Uchihashi Takayuki、Suzuki Daisuke
  • Journal Title: Angew. Chem. Int. Ed. Volume: 56 Pages: 12146-12149
  • DOI: 10.1002/anie.201705808
  • Peer Reviewed
• [Journal Article] Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis (2017)
  • Author(s): Harada Hirofumi、Onoda Akira、Uchihashi Takayuki、Watanabe Hiroki、Sunagawa Naoki、Samejima Masahiro、Igarashi Kiyohiko、Hayashi Takashi
  • Journal Title: Chemical Science Volume: 8 Pages: 6561-6565
  • DOI: 10.1039/c7sc01672g
  • Peer Reviewed
• [Journal Article] Visualisation of a flexible modular structure of the ER folding-sensor enzyme UGGT (2017)
  • Author(s): Satoh Tadashi、Song Chihong、Zhu Tong、Toshimori Takayasu、Murata Kazuyoshi、Hayashi Yugo、Kamikubo Hironari、Uchihashi Takayuki、Kato Koichi
  • Journal Title: Sci. Rep. Volume: 7 Pages: 12142-12142
  • DOI: 10.1038/s41598-017-12283-w
  • Peer Reviewed / Open Access
• [Journal Article] Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy (2017)
  • Author(s): Shibata Mikihiro、Nishimasu Hiroshi、Kodera Noriyuki、Hirano Seiichi、Ando Toshio、Uchihashi Takayuki、Nureki Osamu
  • Journal Title: Nat. Commun. Volume: 8 Pages: 1430-1430
  • DOI: 10.1038/s41467-017-01466-8
  • Peer Reviewed / Open Access
• [Journal Article] High-speed atomic force microscopy imaging of live mammalian cells (2017)
  • Author(s): Mikihiro Shibata, Hiroki Watanabe, Takayuki Uchihashi, Toshio Ando, and Ryohei Yasuda
  • Journal Title: Biophys. Physicobiol. Volume: 14 Issue: 0 Pages: 127-135
  • DOI: 10.2142/biophysico.14.0_127
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Na+-induced structural transition of MotPS for stator assembly of Bacillus flagellar motor. (2017)
  • Author(s): Terahara, N., Kodera, N., Uchihashi, T., Ando, T., Namba, K. & Minamino, T.
  • Journal Title: Science Advances Volume: 3 Pages: 4119-4119
  • DOI: 10.1126/sciadv.aao4119
  • Peer Reviewed / Open Access
• [Journal Article] Oriented Reconstitution of the Full-Length KcsA Potassium Channel in a Lipid Bilayer for AFM Imaging. (2017)
  • Author(s): Ayumi Sumino, Takayuki Uchihashi and Shigetoshi Oiki
  • Journal Title: The Journal of Physical Chemistry Letters Volume: 8 Pages: 785-793
  • DOI: 10.1021/acs.jpclett.6b03058
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A natural light-driven inward proton pump. (2016)
  • Author(s): Keiichi Inoue, Shota Ito, Yoshitaka Kato, Yurika Nomura, Mikihiro Shibata, Takayuki Uchihashi, Satoshi P. Tsunoda and Hideki Kandori
  • Journal Title: Nature Communication Volume: 7 Pages: 13415-13415
  • DOI: 10.1038/ncomms13415
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Direct observation of self-assembly process of biological and artificial fibrils using high-speed atomic force microscopy (2018)
  • Author(s): Takayuki Uchihashi
  • Organizer: Interhierarchical understanding of materials and life through molecular observation
  • Int'l Joint Research / Invited
• [Presentation] 生命の構成部品を直接みて理解する ～ 顕微鏡技術で可視化するタンパク質のダイナミクス現象 ～ (2018)
  • Author(s): 内橋貴之
  • Organizer: 自然科学研究機構 機構長プレス懇談会 「生きているとは何か？」～みる・よむ・つくる研究領域～
  • Invited
• [Presentation] Direct visualization of dynamic molecular interactions using HS-AFM (2017)
  • Author(s): Takayuki Uchihashi
  • Organizer: Frontier Bioorganization Forum 2017: Dynamical ordering and integrated functions of biomolecular systems
  • Int'l Joint Research / Invited
• [Presentation] Direct visualization of single molecule dynamics by high-speed atomic force microscopy (2017)
  • Author(s): Takayuki Uchihashi
  • Organizer: Telluride Science Research Center Workshop on Protein Dynamics
  • Int'l Joint Research / Invited
• [Presentation] Oligomeric state and conformational dynamics of eubacterial ion-pumping rhodopsin studied by high-speed AFM (2017)
  • Author(s): Takayuki Uchihashi
  • Organizer: KAKENHI International Symposium on “Studying the Function of Soft Molecular Systems”
  • Int'l Joint Research / Invited
• [Presentation] High-speed atomic force microscopy for a tool to visualize dynamic events on biological systems from single molecules to living cells (2017)
  • Author(s): Takayuki Uchihashi
  • Organizer: Workshop on “Nanofluidics in Biological Systems”
  • Int'l Joint Research / Invited
• [Presentation] Direct observation of single molecule dynamics at work with high-speed atomic force microscopy (2017)
  • Author(s): Takayuki Uchihashi
  • Organizer: Frontier in Single Molecule Biophysics
  • Int'l Joint Research / Invited
• [Presentation] Visualization of Single-Molecule Dynamics Using High-Speed Atomic Force Microscopy (2017)
  • Author(s): Takayuki Uchihashi
  • Organizer: The 2nd Korea-Japan Joint Symposium on Single-Molecule Biophysics 2017
  • Int'l Joint Research / Invited
• [Presentation] High-speed atomic force microscopy: A tool for visualizing dynamic behavior from proteins to cells (2017)
  • Author(s): Takayuki Uchihashi
  • Organizer: The 28th 2017 International Symposium on Micro-NanoMechanical and Human Science
  • Int'l Joint Research / Invited
• [Presentation] In-line Force Measurements with High-speed AFM (2017)
  • Author(s): Christian Ganser, Takayuki Uchihashi
  • Organizer: The 25th International Colloquium on Scanning Probe Microscopy
  • Int'l Joint Research
• [Presentation] Two-step process for disassembly mechanism of proteasome α7 homo-tetradecamer by α6 revealed by high-speed atomic force microscopy (2017)
  • Author(s): Toshiya Kozai, Taichiro Sekiguchi, Tadashi Satoh, Hirokazu Yagi, Koichi Kato and Takayuki Uchihashi
  • Organizer: The 25th International Colloquium on Scanning Probe Microscopy
  • Int'l Joint Research
• [Presentation] Development of new mirro-tilter unit for tip-scanning high-speed atomic force microscopy (2017)
  • Author(s): Hiroki Watanabe, Takayuki Uchihashi and Shinnosuke Yamanaka and Toshio Ando
  • Organizer: The 25th International Colloquium on Scanning Probe Microscopy
  • Int'l Joint Research
• [Presentation] Dynamic Observationb of Kai Proteins by HS-AFM Reveales a Mechanism of the Robustness in the Cyanobacterial Circadian Oscillator (2017)
  • Author(s): Shogo Sugiyama, Tetsuya Mori, Mark Byne, Takayuki Uchihashi, Carl H. Johnson and Toshio Ando
  • Organizer: The 62nd Annual Meeting of Biophysical Society
  • Int'l Joint Research
• [Presentation] 高速AFMで明らかにするKaiタンパク質間の動的相互作用 (2017)
  • Author(s): 内橋貴之
  • Organizer: 第６９回 日本細胞生物学会大会, シンポジウム「分子の集合・離脱がつかさどる動的な細胞機能」
  • Invited
• [Presentation] 高速原子間力顕微鏡で可視化するタンパク質の動的秩序 (2017)
  • Author(s): 内橋貴之
  • Organizer: 第１７回 日本蛋白質科学会年会, ワークショップ「蛋白質動的秩序のマルチプローブを用いた統合的解析
  • Invited
• [Presentation] 高速AFMを用いた生体分子のその場観察 (2017)
  • Author(s): 内橋貴之
  • Organizer: ２０１７年真空・表面科学合同講演会, 合同シンポジウム「バイオ表面・界面，細胞，生体組織のオペランド計測
  • Invited
• [Presentation] 高速原子間力顕微鏡で可視化する生体・人工高分子の動態 (2017)
  • Author(s): 内橋貴之
  • Organizer: ２０１７年真空・表面科学合同講演会, 表面：プローブ顕微鏡研究部会「走査プローブ顕微鏡によるナノ表面科学の最前線」
  • Invited
• [Presentation] 高速原子間力顕微鏡による生体分子のダイナミクス計測 (2017)
  • Author(s): 内橋貴之
  • Organizer: 新世代研究所 バイオ単分子研究会「タンパク質の作動原理の理解へ向けて - 機能する姿を活写する -
  • Invited
• [Presentation] Analysis of Rotational Dynamics of Rotorless Enterococcus hirae V1-ATPase using High-Speed Atomic Force Microscopy (2017)
  • Author(s): Motonori Imamura, Kazuya Nakamoto, Shintaro Maruyama, Fumihiro Kawai, Ryota Iino, Takayuki Uchihashi, Takeshi Murata, Toshio Ando
  • Organizer: 日本生物物理学会第55回年会, シンポジウム "Analysis of Protein Dynamics in Solution"
  • Int'l Joint Research / Invited
• [Presentation] Dynamic remodeling of Dynamin complexes during membrane fission (2017)
  • Author(s): Tetsuya Takeda, Daiki Ishikuro, Huiran Yang, Toshiya Kozai, Kaho Seyama, Yusuke Kumagai, Hiroshi Yamada, Takayuki Uchihashi, Toshio Ando, Kohji Takei
  • Organizer: 日本生物物理学会第55回年会, シンポジウム "Biophysics of molecular assembly and biological membrane"
  • Int'l Joint Research / Invited
• [Presentation] High-Speed AFM Observation of Domain Flexibility Related to Enzymatic Function of CRISPR-Cas9 (2017)
  • Author(s): Takayuki Uchihashi, Mikihiro Shibata, Hiroshi Nishimasu, Noriyuki Kodera, Seiichi Hirano, Toshio Ando, Osamu Nureki
  • Organizer: 第54回日本生物物理学会年会 シンポジウム: "Softness and functions of biological molecules under various environments"
  • Int'l Joint Research / Invited
• [Presentation] 高速原子間力顕微鏡で可視化する生体膜反応ダイナミクス (2017)
  • Author(s): 内橋貴之
  • Organizer: 2017年度 生命科学系合同年次大会 ワークショップ「最先端の表面科学手法による生体膜反応の実動作下計測」
  • Invited
• [Presentation] 高速原子間力顕微鏡で可視化する Kai タンパク質間相互作用のダイナミクス (2017)
  • Author(s): 内橋貴之
  • Organizer: 第２４回日本時間生物学会学術大会 シンポジウム「２４時間の創出原理」
  • Invited
• [Presentation] Structural Flexibility and Chaperone Activity of TClpB revealed by High-Speed AFM (2017)
  • Author(s): Takayuki Uchihashi, Yo-hei Watanabe, Ryota Iino and Toshio Ando
  • Organizer: XIX. Annual Linz Winter Workshop
  • Place of Presentation: Sommerhotel Julius-Raab-Heim, Linz, Austria
  • Year and Date: 2017-02-03
  • Int'l Joint Research / Invited
• [Presentation] Visualization of protein molecules in action by high-speed atomic force microscopy (2016)
  • Author(s): Mikihiro Shibata, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando
  • Organizer: 日本生物物理学会第５４回年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
  • Invited
• [Presentation] Direct Visualization of Single Molecule Dynamics at Work with High-Speed Atomic Force Microscopy (2016)
  • Author(s): Takayuki Uchihashi
  • Organizer: BioNANO 2016
  • Place of Presentation: Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland
  • Year and Date: 2016-11-22
  • Int'l Joint Research / Invited
• [Presentation] タンパク質のダイナミクスを可視化する高速原子間力顕微鏡 (2016)
  • Author(s): 内橋貴之
  • Organizer: 生体ボリュームイメージング研究部会＆生理研研究会合同ワークショップ「電子顕微鏡ビッグデータが拓くバイオメディカルサイエンス」～限界を超えるための顕微鏡技術～
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2016-11-17
  • Invited
• [Presentation] 高速原子間力顕微鏡で可視化する生体分子のダイナミクス (2016)
  • Author(s): 内橋貴之
  • Organizer: 理研シンポジウム: 第4回「光量子工学研究 -若手・中堅研究者から見た光量子工学の展開-」
  • Place of Presentation: 理化学研究所
  • Year and Date: 2016-11-01
  • Invited
• [Presentation] 高速AFMによる生体分子ダイナミクスのその場観察 (2016)
  • Author(s): 内橋貴之
  • Organizer: 日本物理学会2016年秋季大会 領域9シンポジウム「表面界面ナノ構造のその場観察」
  • Place of Presentation: 金沢大学角間キャンパス
  • Year and Date: 2016-09-15
  • Invited
• [Presentation] 高速AFMによる生体試料の動態イメージング (2016)
  • Author(s): 内橋貴之
  • Organizer: 第25回日本バイオイメージング学会学術集会 シンポジウム3「ナノバイオイメージング：１分子から細胞までの先端手法」
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2016-09-06
  • Invited
• [Presentation] Dynamic interaction between Kai proteins dependent on phosphorylation states of KaiC revealed by HS-AFM (2016)
  • Author(s): Takayuki Uchihashi, Tetsuya Mori, Shogo Sugiyama, Carl H. Johnson, and Toshio Ando
  • Organizer: 4th Kanazawa Bio-AFM Workshop 2016
  • Place of Presentation: KKRホテル金沢
  • Year and Date: 2016-09-03
  • Int'l Joint Research / Invited
• [Presentation] Direct Visualization of Single Molecule Dynamics by High-Speed Atomic Force Microscopy (2016)
  • Author(s): Takayuki Uchihashi, Noriyuki Kodera and Toshio Ando
  • Organizer: Gordon Research Conference, Single Molecule Approach to Biology
  • Place of Presentation: Chinese University of Hong Kong, Hong Kong
  • Year and Date: 2016-07-03
  • Int'l Joint Research / Invited
• [Presentation] Visualization of Functional Dynamics of Biological Molecules by High-Speed AFM (2016)
  • Author(s): Takayuki Uchihashi
  • Organizer: 分子研研究会 “超機能分子の創成 合成、計測、数理が織りなす社会実装分子の戦略的設計と開発”
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2016-06-27
  • Invited
• [Presentation] 高速原子間力顕微鏡で調べる回転分子モーターの構造ダイナミクス (2016)
  • Author(s): 内橋貴之
  • Organizer: 第１６回 日本蛋白質科学会年会 ワークショップ”回転分子モーターを調べてつくって理解する”
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2016-06-09
  • Invited
• [Presentation] 高速AFMによる膜タンパク質のダイナミクス観察 (2016)
  • Author(s): 内橋貴之
  • Organizer: 蛋白研セミナー ”膜タンパク質の構造ダイナミクス”
  • Place of Presentation: 大阪大学蛋白質科学研究所
  • Year and Date: 2016-05-12
  • Invited
• [Presentation] Direct Observation of Single Molecule Dynamics at Work with High-Speed Atomic Force Microscopy (2016)
  • Author(s): Takayuki Uchihashi
  • Organizer: Les Houches-TSRC Workshop on Protein Dynamics
  • Place of Presentation: Ecole de Physique des Houches, France
  • Year and Date: 2016-04-03
  • Int'l Joint Research / Invited
• [Book] "High-Speed Atomic Force Microcopy in "Compendium of Surface and Interface Analysis (2018)
  • Author(s): Takayuki Uchihashi
  • Publisher: Springer
• [Book] パリティ、「高速原子間力顕微鏡によるタンパク質の動画撮影」 (2018)
  • Author(s): 内橋貴之
  • Publisher: 丸善出版
• [Patent(Industrial Property Rights)] 走査型プローブ顕微鏡 (2016)
  • Inventor(s): 内橋貴之，柴田幹大, 古寺哲幸
  • Industrial Property Rights Holder: 内橋貴之，柴田幹大, 古寺哲幸
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-234584
  • Filing Date: 2016-12-01
  • Application Year: 2016
• [Patent(Industrial Property Rights)] 昇温ホルダおよびプローブ顕微鏡 (2016)
  • Inventor(s): 内橋貴之，足立彗, 古寺哲幸
  • Industrial Property Rights Holder: 内橋貴之，足立彗, 古寺哲幸
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-233494
  • Filing Date: 2016-11-30
  • Application Year: 2016
• [Patent(Industrial Property Rights)] チャンバーアレイの製造方法 (2016)
  • Inventor(s): 古寺哲幸，豊田貴大，内橋貴之
  • Industrial Property Rights Holder: 古寺哲幸，豊田貴大，内橋貴之
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-232100
  • Filing Date: 2016-11-30
  • Application Year: 2016