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人工脂質二重膜におけるドメイン構造の実験的探求

Publicly Offered Research

Project AreaDynamical ordering of biomolecular systems for creation of integrated functions
Project/Area Number 16H00782
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionGakushuin University

Principal Investigator

岩田 耕一  学習院大学, 理学部, 教授 (90232678)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords脂質二重膜 / ドメイン / 分光実験
Outline of Annual Research Achievements

細胞内での生化学反応の多くは,膜タンパク質を触媒として生体膜の内部やその近傍で進行する.生体膜の構造とその時間変化は,そこで進行する生化学反応にきわめて大きな影響を与える.有力な生体膜の構造モデルである「脂質ラフト」は,脂質二重膜がドメイン構造を有することを仮定している.「脂質ラフト」モデルの妥当性を検証することは,現代の科学に課せられた大きな問題の一つである.本研究では,先端的な分光法を実験手段として,脂質二重膜におけるドメイン構造の有無を検証することを主な目的とした.
本年度は,主としてピコ秒時間分解けい光分光法を利用して,人工脂質二重膜内部および細胞膜内部での粘性を評価した.研究代表者がすでに製作していたピコ秒時間分解けい光分光計を本研究のために用いた.脂質二重膜の特定の深さにおける粘度を評価するために,飽和アルキル鎖のスペーサーによってtrans-スチルベンと極性基が結合したけい光プローブを開発した.このけい光プローブを混合したリン脂質が形成した脂質二重膜の時間分解けい光分光測定を行うことによって,特定の深さに固定されたtrans-スチルベンの周囲の粘度を見積った.その結果をもとにして,人工脂質二重膜で観測される粘度の不均一性が膜の水平方向の不均一性に起因する可能性が大きいことを明らかにできた.
本研究課題を遂行する際に,A02班の大谷亮班員およびA03班の申惠媛班員との共同研究を進めた.大谷班員との共同研究では,人工脂質二重膜中に配置したドナーとアクセプターの間でのエネルギー移動に対して膜の物性が与える影響を検討した.申班員との共同研究では,HeLa細胞における細胞膜の粘度を評価するための新たな分光測定法を開発した.これらの領域内共同研究を通じて広範囲の試料に対する分光実験を行うことで,多様な脂質二重膜が示す物性を明らかにすることができた.

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (29 results)

All 2018 2017 2016 Other

All Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Acknowledgement Compliant: 1 results) Presentation (20 results) (of which Int'l Joint Research: 14 results,  Invited: 16 results) Book (1 results) Remarks (1 results)

  • [Journal Article] The effect of regioisomerism on the photophysical properties of alkylated-naphthalene liquids2018

    • Author(s)
      Narayan B.、Nagura K.、Takaya T.、Iwata K.、Shinohara A.、Shinmori H.、Wang H.、Li Q.、Sun X.、Li H.、Ishihara S.、Nakanishi T.
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 20 Issue: 5 Pages: 2970-2975

    • DOI

      10.1039/c7cp05584f

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Experimental and theoretical investigation of fluorescence solvatochromism of dialkoxyphenyl-pyrene molecules2018

    • Author(s)
      Lu Fengniu、Kitamura Naoki、Takaya Tomohisa、Iwata Koichi、Nakanishi Takashi、Kurashige Yuki
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 20 Issue: 5 Pages: 3258-3264

    • DOI

      10.1039/c7cp06811e

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vibrational relaxation dynamics of β-carotene and its derivatives with substituents on terminal rings in electronically excited states as studied by femtosecond time-resolved stimulated Raman spectroscopy in the near-IR region2018

    • Author(s)
      Takaya Tomohisa、Anan Masato、Iwata Koichi
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 20 Issue: 5 Pages: 3320-3327

    • DOI

      10.1039/c7cp06343a

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel physical chemistry approaches in biophysical researches with advanced application of lasers: Detection and manipulation2018

    • Author(s)
      Iwata Koichi、Terazima Masahide、Masuhara Hiroshi
    • Journal Title

      BBA

      Volume: 1862 Issue: 2 Pages: 335-357

    • DOI

      10.1016/j.bbagen.2017.11.003

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The impact of metal complex lipids on viscosity and curvature of hybrid liposomes2017

    • Author(s)
      R. Ohtani, T. Tokita, T. Takaya, K. Iwata, M. Kinoshita, N. Matsumori, M. Nakamura, L. F. Lindoy, S. Hayami
    • Journal Title

      Chem. Commun.

      Volume: 53 Issue: 99 Pages: 13249-13252

    • DOI

      10.1039/c7cc07944c

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A Guide to Design Functional Molecular Liquids with Tailorable Properties using Pyrene-Fluorescence as a Probe2017

    • Author(s)
      Fengniu Lu, Tomohisa Takaya, Koichi Iwata, Izuru Kawamura, Akinori Saeki, Masashi Ishii, Kazuhiko Nagura, Takashi Nakanishi
    • Journal Title

      Scientific Reports

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a femtosecond time-resolved near-IR multiplex stimulated Raman spectrometer in resonance with transitions in the 900-1550 nm region2016

    • Author(s)
      Tomohisa Takaya, Koichi Iwata
    • Journal Title

      Analyst

      Volume: 141 Issue: 14 Pages: 4283-4292

    • DOI

      10.1039/c6an01051b

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Characterizing Lipid Bilayer Membranes with time-resolved Spectroscopies - Viscosity and Polarity2018

    • Author(s)
      Koichi Iwata
    • Organizer
      The 14th Biennial DAE-BRNS Trombay Symposium on Radiation and Photochemistry
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Characterization of Lipid Bilayer Membranes with Time-Resolved Spectroscopy2018

    • Author(s)
      Koichi Iwata
    • Organizer
      The 10th Asian Conference on Ultrafast Phenomena
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Spectroscopy of Molecules Favoured by Raman Followers2018

    • Author(s)
      Koichi Iwata
    • Organizer
      90 Years of Raman Effect: Current Status and Future Directions
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 化学反応を追跡するための分光実験2018

    • Author(s)
      岩田 耕一
    • Organizer
      神奈川大学理学部化学科シンポジウム「化学のための最先端光科学」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Properties of liposome lipid bilayer membranes examined by time-resolved Raman and fluorescence spectroscopies2017

    • Author(s)
      Koichi Iwata
    • Organizer
      3rd Symposium on Weak Molecular Interactions
    • Place of Presentation
      Opole, Poland
    • Year and Date
      2017-03-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] スチルベン修飾脂肪酸を用いて評価したDMPCリポソーム脂質二重膜中の粘度の深さ依存性2017

    • Author(s)
      林春菜,北村捷,髙屋智久,Manjusha Joshi,中村浩之,岩田耕一
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      横浜市
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] ピコ秒時間分解ラマン分光法で測定したDPPC二重膜中でのエネルギー移動過程-コレステロール添加の効果2017

    • Author(s)
      北村捷,髙屋智久,岩田耕一
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      横浜市
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] How we examine lipid bilayer membranes as field of chemical reaction with time-resolved spectroscopy2017

    • Author(s)
      Koichi Iwata
    • Organizer
      International Workshop Present and Future of Ultrafast Spectroscopy
    • Place of Presentation
      Wako, Japan
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Resonance Raman Spectroscopy of “Loose Electrons” with Stimulated Raman Scattering in Near-infrared2017

    • Author(s)
      Koichi Iwata
    • Organizer
      9th International Conference on Advanced Vibrational Spectroscopy
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Electronic and Vibrational Relaxation Examined with Femtosecond Time-resolved Absorption and Stimulated Raman Spectroscopy in Near-infrared2017

    • Author(s)
      Koichi Iwata
    • Organizer
      5th Taiwan International Symposium on Raman Spectroscopy
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Basic Theory for Non-linear Raman Processes2017

    • Author(s)
      Koichi Iwata
    • Organizer
      Taiwan Association of Raman Spectroscopy Summer Camp
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Examining Lipid Bilayer Membranes with Time-resolved Spectroscopies2017

    • Author(s)
      Koichi Iwata
    • Organizer
      6th Asian Spectroscopy Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Loose electrons traced with time-resolved near-infrared spectroscopy - absorption and non-linear Raman2017

    • Author(s)
      Koichi Iwata
    • Organizer
      International Conference on Spectroscopy of Biomolecules and Advanced Materials
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 特異な化学反応場としての脂質二重膜-時間分解分光法による特性評価2017

    • Author(s)
      岩田 耕一
    • Organizer
      第11回分子科学討論会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 時間分解分光法でみる化学反応2017

    • Author(s)
      岩田 耕一
    • Organizer
      公開講演会 最新化学談話シリーズ,九州大学理学部化学教室談話会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Effect of cholesterol on thermal diffusion in DPPC lipid bilayers observed with picosecond time-resolved Raman spectroscopy2016

    • Author(s)
      Sho Kitamura, Tomohisa Takaya, Koichi Iwata
    • Organizer
      6th International Conference on Perspectives in Vibrational Spectroscopy
    • Place of Presentation
      Lucknow, India
    • Year and Date
      2016-11-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Local environment in ionic liquids and lipid bilayer membranes examined with Raman spectroscopy2016

    • Author(s)
      Koichi Iwata
    • Organizer
      6th International Conference on Perspectives in Vibrational Spectroscopy
    • Place of Presentation
      Lucknow, India
    • Year and Date
      2016-11-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] What we see by time-resolving in near-IR2016

    • Author(s)
      Koichi Iwata
    • Organizer
      SciX2016
    • Place of Presentation
      Minneapolis, MN, USA
    • Year and Date
      2016-09-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Effect of Cholesterol on Lipid Bilayer Membranes Estimated with Picosecond Time-Resolved Raman Spectroscopy2016

    • Author(s)
      Sho Kitamura, Tomihisa Takaya, Koichi Iwata
    • Organizer
      XXVth International Conference on Raman Spectroscopy
    • Place of Presentation
      Fortaleza, Brazil
    • Year and Date
      2016-08-15
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イオン液体における巨視的物性と微視的溶液内環境の関係2016

    • Author(s)
      岩田耕一
    • Organizer
      イオン液体の分子科学~基礎からひも解く物性、機能~
    • Place of Presentation
      東京都目黒区
    • Year and Date
      2016-06-24
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Book] Encyclopedia of Spectroscopy and Spectrometry, 3rd Edition2016

    • Author(s)
      Hiro-o Hamatuchi, Koichi Iwata
    • Publisher
      Elsevier
    • Related Report
      2016 Annual Research Report
  • [Remarks] 岩田研究室ホームページ

    • URL

      http://www-cc.gakushuin.ac.jp/~20040130/index.htm

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report

URL: 

Published: 2016-04-26   Modified: 2018-12-17  

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