細胞質流動から迫る細胞質におけるゆらぎから構造が生じるメカニズム

• Project Area: Synergy of Fluctuation and Structure:Foundation of Universal Laws in Nonequilibrium Systems
• Project/Area Number: 16H00816
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: National Institute of Genetics
• Principal Investigator: 木村 暁 国立遺伝学研究所, 構造遺伝学研究センター, 教授 (10365447)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 線虫 / 自己組織化 / フィードバック制御 / ゆらぎ / 微小管 / 細胞質流動 / モータータンパク質

Outline of Annual Research Achievements

細胞質流動は細胞内で細胞質全体が流れるように移動する集団的な現象である。本研究課題では、流動場が自発的に形成される３つのタイプ（Circulation型、Saltation型、Rotation型）の細胞質流動を統一的に「ゆらぎと構造の協奏」のコンセプトから説明するモデルを構築する。そして、その妥当性を実験的に検証することを目的とする。本研究では線虫(C. elegans)の「減数分裂時細胞質流動(MeiCS)」に着目して研究をすすめた。昨年度末までに３つのタイプを説明するモデルの構築と、流動の原動力を発生する微小管の長さを制御することによってCirculation型からRotation型への変換が起こることを実験的に検証できた(Kimura K., et al., Nat Cell Biol, 2017)。そこで、本年度はSaltation型からCirculation型への変換を実験的に再現することを試みた。具体的には、微小管の上を移動し、流動を引き起こすモータータンパク質の活性を変化させることによって変換させることができるかどうかについて検討を行った。これまでに、変換が生じることを確認しているが、定量的な解析については現在進行中で、確定的な結果を得るまでにはさらなる研究が必要である。

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] キュリー研究所(フランス)
• [Int'l Joint Research] EMBL(ドイツ)
• [Int'l Joint Research] キュリー研究所(フランス)
• [Int'l Joint Research] EMBL(ドイツ)
• [Journal Article] Tumor suppressor APC is an attenuator of spindle-pulling forces duringC. elegansasymmetric cell division (2018)
  • Author(s): Sugioka Kenji、Fielmich Lars-Eric、Mizumoto Kota、Bowerman Bruce、van den Heuvel Sander、Kimura Akatsuki、Sawa Hitoshi
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 115 Issue: 5
  • DOI: 10.1073/pnas.1712052115
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An asymmetric attraction model for the diversity and robustness of cell arrangement in nematodes (2017)
  • Author(s): Yamamoto Kazunori、Kimura Akatsuki
  • Journal Title: Development Volume: 144 Issue: 23 Pages: 4437-4449
  • DOI: 10.1242/dev.154609
  • Peer Reviewed / Open Access
• [Journal Article] Reduction in chromosome mobility accompanies nuclear organization during early embryogenesis in Caenorhabditis elegans (2017)
  • Author(s): Arai Ritsuko、Sugawara Takeshi、Sato Yuko、Minakuchi Yohei、Toyoda Atsushi、Nabeshima Kentaro、Kimura Hiroshi、Kimura Akatsuki
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 3631-3631
  • DOI: 10.1038/s41598-017-03483-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Physical properties of the chromosomes and implications for development (2017)
  • Author(s): Sugawara Takeshi、Kimura Akatsuki
  • Journal Title: Development, Growth & Differentiation Volume: 59 Issue: 5 Pages: 405-414
  • DOI: 10.1111/dgd.12363
  • Peer Reviewed
• [Journal Article] Endoplasmic Reticulum-Mediated Microtubule Alignment Governs Cytoplasmic Streaming (2017)
  • Author(s): Kenji Kimura, Alexandre Mamane, Tohru Sasaki, Kohta Sato, Jun Takagi, Ritsuya Niwayama, Lars Hufnagel, Yuta Shimamoto, Jean-Francois Joanny, Seiichi Uchida, and Akatsuki Kimura
  • Journal Title: Nature Cell Biology Volume: 19 Issue: 4 Pages: 399-406
  • DOI: 10.1038/ncb3490
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A genetically encoded probe for live-cell imaging of H4K20 monomethylation (2016)
  • Author(s): Sato Y., Kujirai T., Arai R., Asakawa H., Ohtsuki C., Horikoshi N., Yamagata K., Ueda J., Nagase T., Haraguchi T., Hiraoka Y., Kimura A., Kurumizaka H., Kimura, H.
  • Journal Title: J. Mol. Biol. Volume: 428 Issue: 20 Pages: 3885-3902
  • DOI: 10.1016/j.jmb.2016.08.010
  • Peer Reviewed / Open Access
• [Journal Article] How Does a Cell’s Nucleus Know Where the Center of the Cell Is? (2016)
  • Author(s): 谷本博一, 木村健二, 木村暁
  • Journal Title: Seibutsu Butsuri Volume: 56 Issue: 5 Pages: 271-274
  • DOI: 10.2142/biophys.56.271
  • NAID: 130005264927
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Bayesian Inference of Forces Causing Cytoplasmic Streaming in Caenorhabditis elegans Embryos and Mouse Oocytes. (2016)
  • Author(s): Niwayama R., Nagao H., Kitajima T. S., Hufnagel L., Shinohara K., Higuchi T., Ishikawa T., Kimura A.
  • Journal Title: PLoS ONE Volume: 11 Issue: 7 Pages: e0159917-e0159917
  • DOI: 10.1371/journal.pone.0159917
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Interphase adhesion geometry is transmitted to an internal regulator for spindle orientation via caveolin-1. (2016)
  • Author(s): Matsumura S., Kojidani T., Kamioka Y., Uchida S., Haraguchi T., Kimura A., and Toyoshima F.
  • Journal Title: Nature Communications Volume: 7 Issue: 1 Pages: 11858-11858
  • DOI: 10.1038/ncomms11858
  • NAID: 120005774665
  • Peer Reviewed / Open Access
• [Presentation] Measurement of intracellular forces using centrifuge polarization microscope (CPM). (2017)
  • Author(s): Akatsuki Kimura
  • Organizer: International Workshop on Quantitative Biology 2017
  • Int'l Joint Research / Invited
• [Presentation] バイオ画像解析支援をうけた細胞内の力発生メカニズムの理解 (2017)
  • Author(s): 木村暁
  • Organizer: 生命科学４プラットフォーム成果シンポジウム
  • Invited
• [Presentation] 細胞核はどうして細胞の中心に配置できるのか？ －遺伝学、計算、マイクロデバイス、遠心顕微鏡を使った解析－ (2017)
  • Author(s): 木村暁
  • Organizer: 日本大学文理学部生命科学セミナー「細胞核機能の発現と制御」
  • Invited
• [Presentation] Emergence and reversal of cytoplasmic streaming in the C. elegance nematode zygote. (2017)
  • Author(s): Akatsuki Kimura
  • Organizer: International workshop on Active Soft Matter and Ethology
  • Int'l Joint Research / Invited
• [Presentation] Mechanism of spontaneous alignment of microtubules during meiotic cytoplasmic streaming in the C. elegans zygote. (2017)
  • Author(s): Akatsuki Kimura
  • Organizer: Hierarchy of biological functions connected by concept of active matter
  • Int'l Joint Research / Invited
• [Presentation] A self-organization model for the emergence and reversal of meiotic cytoplasmic streaming in the C. elegans embryo. (2017)
  • Author(s): Akatsuki Kimura
  • Organizer: International Symposium on Fluctuation and Structure out of Equilibrium 2017 (SFS2017)
  • Int'l Joint Research / Invited
• [Presentation] 核の細胞内配置を（数理モデルで）造る・（実験的に）操作する (2017)
  • Author(s): 木村暁
  • Organizer: 2017年度生命科学系学会合同年次大会（ConBio2017）
• [Presentation] Cytoplasmic Streaming in the nematode, C. elegans. (2017)
  • Author(s): Akatsuki Kimura
  • Organizer: Active Matter Workshop 2018
  • Int'l Joint Research
• [Presentation] Imaging and modeling of cytoplasmic streaming in the C. elegans embryo. (2016)
  • Author(s): Akatsuki Kimura
  • Organizer: The 54th Annual Meeting of the BSJ
  • Place of Presentation: Tsukuba
  • Invited
• [Remarks] APCは紡錘体を引っ張る力のブレーキ がん抑制遺伝子APCの新しい機能の発見
  • URL: https://www.nig.ac.jp/nig/ja/2018/01/research-highlights_ja/20180119-2.html
• [Remarks] 細胞核内のDNA密度と、分裂期染色体の凝縮度との間の種を越えた相関関係
  • URL: https://www.nig.ac.jp/nig/ja/2017/12/research-highlights_ja/20171220.html
• [Remarks] 初期胚発生過程における染色体の動きの変化を検出
  • URL: https://www.nig.ac.jp/nig/ja/2017/12/research-highlights_ja/20171220-2.html
• [Remarks] はじまりは卵の形だった～初期胚における細胞の配置パターンの決定機構～
  • URL: https://www.nig.ac.jp/nig/ja/2017/11/research-highlights_ja/20171129.html
• [Remarks] 流行が作られるしくみ「同調現象」を、細胞の中で発見―細胞質流動の生成と逆転のメカニズム―
  • URL: https://www.nig.ac.jp/nig/ja/2017/03/research-highlights_ja/20170314.html
• [Remarks] プレスリリース
  • URL: https://www.nig.ac.jp/nig/images/research_highlights/PR20170314.pdf
• [Remarks] 研究室ウェブサイト
  • URL: https://www.nig.ac.jp/nig/ja/research/organization-top/laboratories/kimura