固体NMRによる膜タンパク質の機能的な相互作用の観測

• Project Area: Science on Function of Soft Molecular Systems by Cooperation of Theory and Experiment
• Project/Area Number: 16H00828
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Yokohama National University
• Principal Investigator: 川村 出 横浜国立大学, 大学院工学研究院, 准教授 (20452047)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 固体NMR / レチナール / ロドプシン / 抗菌ペプチド / Dアミノ酸 / 自己組織化 / ペプチド / 膜タンパク質 / 化学シフト / 固体NMR分光法 / 安定同位体標識

Outline of Annual Research Achievements

細胞膜中に存在する膜タンパク質などの柔らかな分子に関して、固体核磁気共鳴(NMR)法を駆使して構造解析を行い、本年度は次のような重要な成果をあげた。
1. 固体NMR, VCD及びMDシミュレーションなどを駆使して、D-アミノ酸残基を有する抗菌性ペプチド ボンビニンH4と細胞膜との相互作用様式を詳細に明らかにし、高い活性を発現するためのDアミノ酸の役割を示した。特に、Dアミノ酸が存在することによって、N端側から2残基の疎水性側鎖がシス型の構造になり、これが脂質二重膜の疎水領域にアンカーとして相互作用することで膜貫通状態に移行しやすいことを示した。これは全てL体のアミノ酸で構成されるボンビニンH2では見られないため、Dアミノ酸の役割を分子レベルで示したユニークな成果である。2. 微生物型ロドプシンの一つである光駆動型ナトリウムイオンポンプロドプシンの細胞外側ナトリウム結合サイトの構造変化と反応中心であるレチナール結合サイトの長距離の相互作用を明らかにし、ポンプ活性にナトリウム結合サイトの構造が関与していることを示した。特にH30A変異体のナトリウムイオン非存在条件ではシッフ塩基と対イオンであるAsp116の静電相互作用が強まることが明らかとなった。3. 特定の条件で短いペプチドが自己組織化してナノファイバーは様々な応用が期待されている。本研究によってナノファイバーを形成した際の分子構造を緻密に調べるための固体NMR実験を実証した。

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] National University of Mongolia(モンゴル)
• [Journal Article] Long-distance perturbation on Schiff base-counterion interactions by His30 and the extracellular Na+-binding site in Krokinobacter rhodopsin 2 (2018)
  • Author(s): Arisu Shigeta, Shota Ito, Rina Kaneko, Sahoko Tomida, Keiichi Inoue, Hideki Kandori, Izuru Kawamura
  • Journal Title: Physical Chemistry Chemical Physics Volume: 20 Issue: 13 Pages: 8450-8455
  • DOI: 10.1039/c8cp00626a
  • Peer Reviewed
• [Journal Article] The role of d - allo -isoleucine in the deposition of the anti- Leishmania peptide bombinin H4 as revealed by 31 P solid-state NMR, VCD spectroscopy, and MD simulation (2018)
  • Author(s): Mijiddorj Batsaikhan、Kaneda Shiho、Sato Hisako、Kitahashi Yuki、Javkhlantugs Namsrai、Naito Akira、Ueda Kazuyoshi、Kawamura Izuru
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics Volume: 1866 Issue: 7 Pages: 789-798
  • DOI: 10.1016/j.bbapap.2018.01.005
  • NAID: 120006649291
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Electrooxidative Copolymerization of 3,4-Ethylenedioxithiophene and Benzene from a Mixture of Each Monomer (2018)
  • Author(s): Tanaka Eisaku、Tateno Hiroyuki、Matsumura Yoshimasa、Nakabayashi Koji、Kawamura Izuru、Atobe Mahito
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 91 Issue: 2 Pages: 141-146
  • DOI: 10.1246/bcsj.20170319
  • NAID: 130006350290
  • ISSN: 0009-2673, 1348-0634
  • Peer Reviewed
• [Journal Article] Absorption of Cu(II) in layered diaminoalkyl- and monoaminoalkyl-polysilsesquioxane (2017)
  • Author(s): Teppei Takagi, Izuru Kawamura, Yasunori Oumi, Yohei Miwa, Hideaki Yoshitake
  • Journal Title: Polymer Volume: 132 Pages: 227-234
  • DOI: 10.1016/j.polymer.2017.11.011
  • Peer Reviewed
• [Journal Article] A Guide to Design Functional Molecular Liquids with Tailorable Properties using Pyrene-Fluorescence as a Probe (2017)
  • Author(s): Fengniu Li, Tomohisa Takaya, Koichi Iwata, Izuru Kawamura, Akinori Saeki, Masashi Ishii, Kazuhiko Nagura, Takashi Nakanishi
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 3416-3416
  • DOI: 10.1038/s41598-017-03584-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Solid-state NMR structural study of retinal-binding pocket in sodium ion pump rhodopsin. (2017)
  • Author(s): Arisu Shigeta, Shota Ito, Keiichi Inoue, Takashi Okitsu, Akimori Wada, Hideki Kandori, Izuru Kawamura*
  • Journal Title: Biochemistry Volume: 56 Issue: 4 Pages: 543-550
  • DOI: 10.1021/acs.biochem.6b00999
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Solid State VCD spectra of Isoleucine and its Related Compounds: Effects of Interplay between Two Chiral Centers. (2017)
  • Author(s): Hisako Sato*, Izuru Kawamura, Akihiko Yamagishi, Fumi Sato
  • Journal Title: Chemistry Letters (Editor's choice) Volume: 46 Issue: 4 Pages: 449-452
  • DOI: 10.1246/cl.161043
  • NAID: 130005549111
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dynamic structure and orientation of melittin bound to acidic lipid bilayers, as revealed by solid-state NMR and molecular dynamic simulation (2017)
  • Author(s): K. Norisada, N. Javkhlantugs, D. Mishima, I. Kawamura, H. Saito, K. Ueda, A. Naito
  • Journal Title: J. Phys. Chem. B. Volume: 121 Issue: 8 Pages: 1802-1811
  • DOI: 10.1021/acs.jpcb.6b11207
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Magnetically Alignable Bicelles with Unprecedented Stability Using Tunable Surfactants Derived from Cholic Acid (2016)
  • Author(s): Ryoichi Matsui, Noriyuki Uchida, Masataka Ohtani, Kuniyo Yamada, Arisu Shigeta, Izuru Kawamura, Takuzo Aida, Yasuhiro Ishida*
  • Journal Title: ChemPhysChem Volume: 17 Issue: 23 Pages: 3916-3922
  • DOI: 10.1002/cphc.201600897
  • Peer Reviewed
• [Presentation] Structural change in retinal- and sodium ion-binding site of Na+ pump rhodopsin at neutral and acidic pH studied by solid-state NMR (Poster) (2017)
  • Author(s): A. Shigeta, S. Ito, K. Inoue, T. Okitsu, A. Wada, H. Kandori, I. Kawamura
  • Organizer: The 7th Asia-Pacific NMR Symposium,
  • Place of Presentation: Indian Institute of Science, Bangalore
  • Year and Date: 2017-02-16
  • Int'l Joint Research
• [Presentation] Structural change of Na+ pumping Krokinobacter rhodopsin 2 induced by pH and alkali metal ions by solid-state NMR (2017)
  • Author(s): Arisu Shigeta, Rina Kaneko, Shota Ito, Takashi Okitsu, Akimori Wada, Keiichi Inoue, Hideki Kandori, Izuru Kawamura
  • Organizer: 第56回NMR討論会
• [Presentation] センサリーロドプシンのレチナール構造変化に対する13C NMR化学シフト値計算 (2017)
  • Author(s): 槇野義輝, 川村出, 沖津貴志, 和田昭盛, 須藤雄気, 加茂直樹, 内藤晶, 上田一義
  • Organizer: 第56回NMR討論会
• [Presentation] 抗菌ペプチドHylin b1のpH変化に対する運動性の違い (2017)
  • Author(s): 川村出, 廣重玲奈, Batsaikhan Mijiddorj, 松尾雄大, 上田一義
  • Organizer: 第56回NMR討論会
• [Presentation] 固体NMRによるPhe-Pheモチーフをもつペプチドの自己組織化集合体の分子構造の解析 (2017)
  • Author(s): 尾澤夢実, Batsaikhan Mijiddorj, 佐藤久子, 内藤晶, 川村出
  • Organizer: 第56回NMR討論会
• [Presentation] Stationary trapping of photo-intermediates during the photo cycles of bR and its mutants by in situ photoirradiation solid-state NMR (2017)
  • Author(s): Akira Naito, Kyosuke Oshima, Yuto Otani, Arisu Shigeta, Yoshiteru Makino, Izuru Kawamura, Takashi Okitsu, Akimori Wada, Satoru Tuzi, Tatsuo Iwasa
  • Organizer: 第55回日本生物物理学会
• [Presentation] Membrane binding structure of bombinin H2 and H4 peptides in leishmania mimetic membrane as studied by solid-state NMR and MD simulation (2017)
  • Author(s): Batsaikhan Mijiddorj, Shiho Kaneda, Namsrai Javkhlantugs, Kazuyoshi Ueda, Hisako Sato, Akira Naito, Izuru Kawamura
  • Organizer: 第55回日本生物物理学会
• [Presentation] Quantum chemical calculation of 13C NMR chemical shifts for retinal at photo-intermediates in the photocycle of sensory rhodopsin (2017)
  • Author(s): Yoshiteru Makino, Izuru Kawamura, Takashi Okitsu, Akimori Wada, Yuki Sudo, Naoki Kamo, Akira Naito, Kazuyoshi Ueda
  • Organizer: 第55回日本生物物理学会
• [Presentation] Photo-intermediates in the photo-reaction pathways of bacteriorhodopsin as revealed by in situ photo-irradiation solid-state NMR (2017)
  • Author(s): Akira Naito, Kyosuka Oshima, Yuto Otani, Arisu Shigeta, Yoshiteru Makino, Izuru Kawamura, Takashi Okitsu, Akimori Wada, Satoru Yuzi, Tatsuo Iwasa
  • Organizer: The 20th ISMAR
  • Int'l Joint Research
• [Presentation] Structural analysis of retinal-binding pocket of KR2 under neutral and acidic PH by solid-state NMR (2017)
  • Author(s): Arisu Shigeta, Yokohama National University, Japan Shota Ito, Takashi Okitsu, Akimori Wada, Keiichi Inoue, Hideki Kandori, Izuru Kawamura
  • Organizer: The 20th ISMAR
  • Int'l Joint Research
• [Presentation] Structural difference of functional peptide fibers between L-Phe-L-Phe-OH, L-Phe-D-Phe-OH and D-Phe-D-Phe-OH (2017)
  • Author(s): Yumi Ozawa, Batsaikhan Mijiddorj, Hisako Sato, Namsrai Javkhlantugs, Akira Naito, Kazuyoshi Ueda, Izuru Kawamura
  • Organizer: The 3rd International Conference on D-Amino Acid Research IDAR2017
  • Int'l Joint Research
• [Presentation] Structure of bombinin H4 containing a D-allo- isoleucine by solid-state VCD and solid-state NMR (2017)
  • Author(s): Izuru Kawamura, Shiho Kaneda, Yuki Kitahashi, Hisako Sato, Akira Naito
  • Organizer: The 3rd International Conference on D-Amino Acid Research IDAR2017
  • Int'l Joint Research
• [Presentation] Membrane interactions of anti-Leishmania peptide Bombinin H4 containing D-allo-isoleucine (2017)
  • Author(s): Batsaikhan Mijiddorj, Shiho Kaneda, Namsrai Javkhlantugs, Akira Naito, Kazuyoshi Ueda, Izuru Kawamura
  • Organizer: The 3rd International Conference on D-Amino Acid Research IDAR2017
  • Int'l Joint Research
• [Presentation] 固体NMRを用いたDアミノ酸含有機能性ペプチドの構造解析 (2017)
  • Author(s): 川村出
  • Organizer: 第17回日本蛋白質科学会年会
  • Invited
• [Presentation] Observation of functional membrane protein interaction by solid-state NMR (2017)
  • Author(s): Izuru Kawamura
  • Organizer: KAKENHI International Symposium on “Studying the Function of Soft Molecular Systems”
  • Int'l Joint Research
• [Presentation] 立体反転によって誘起されるペプチドファイバーの形態変化と固体NMRによる構造解析 (2017)
  • Author(s): 尾澤夢実, 佐藤久子, 内藤晶, 川村出
  • Organizer: 17-1 NMR研究会
• [Presentation] Relationship between Tyr Czeta chemical shift and hydrogen bonding strength in microbial rhodopsins (2017)
  • Author(s): Arisu Shigeta, Izuru Kawamura
  • Organizer: International Symposium on Biophysics of Rhodopsins honoring Prof. Yoshinori Shichida
  • Int'l Joint Research
• [Presentation] 固体NMRを用いた機能性分子液体の構造解析 (2017)
  • Author(s): 川村 出
  • Organizer: 「機能性液体」に関する研究会
  • Place of Presentation: 物質・材料研究機構並木地区
  • Invited
• [Presentation] 固体NMRを用いたミドルロドプシンのレチナール結合ポケットの構造解析 (ポスター) (2016)
  • Author(s): I. Kawamura, H. Seki, A. Shigeta, Y. Makino, T. Okitsu, A. Wada, Y. Sudo
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] 酸性および中性におけるナトリウムイオンポンプKR2のレチナール結合ポケットの固体NMR構造解析 (ポスター) (2016)
  • Author(s): A. Shigeta, S. Ito, T. Okitsu, A. Wada, K. Inoue, H. Kandori, I. Kawamura
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] 固体NMRによるバクテリオロドプシンの暗順応状態におけるTyr185の構造解析 (ポスター) (2016)
  • Author(s): Y. Otani, A. Shigeta, Y. Kebukawa, K. Kobayashi, T. Okitsu, A. Wada, S. Tuzi, A. Naito, I. Kawamura
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] Secondary structural change of glucagon during fibril formation process with DMPC lipid bilayers as revealed by 13C solid-state NMR (ポスター) (2016)
  • Author(s): K. Haya, I. Kawamura, A. Naito
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] D体フェニルアラニンを含む抗菌ペプチドPhenylseptinの脂質膜との相互作用解析 (ポスター) (2016)
  • Author(s): Y. Matsuo, I. Kawamura
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] カチオン性抗菌ペプチド ボンビニンH2およびH4のリーシュマニア原虫模倣膜との特異的な相互作用 (学生発表賞受賞) (2016)
  • Author(s): S. Kaneda, A. Naito, I. Kawamura
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] In-situ光照射固体NMRによるバクテリオロドプシンD96N変異体の光反応過程でのレチナールおよびタンパク質構造変化の解析 (ポスター) (2016)
  • Author(s): 大谷優人、重田安里寿、槙野義輝、宮佐亮太、川村出、沖津貴志、和田昭盛、辻暁、内藤晶
  • Organizer: 第55回NMR討論会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2016-11-16
• [Presentation] 31P固体NMRによる抗菌ペプチドボンビニンH2およびH4の細菌模倣膜に対する作用の解析 (ポスター) (2016)
  • Author(s): 金田志穂、北橋由貴、内藤晶、川村出
  • Organizer: 第55回NMR討論会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2016-11-16
• [Presentation] 固体NMRによる光駆動型ナトリウムイオンポンプKR2のpH変化に対するレチナール結合サイトの構造変化の解析 (大陽日酸賞受賞) (2016)
  • Author(s): 重田安里寿、伊藤奨太、沖津貴志、和田昭盛、井上圭一、神取秀樹、川村出
  • Organizer: 第55回NMR討論会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2016-11-16
• [Presentation] 固体NMRを用いたミドルロドプシンの温度変化に対する構造変化の解析 (ポスター) (2016)
  • Author(s): 川村出、関隼斗、槙野義輝、重田安里寿、沖津貴志、和田昭盛、須藤雄気
  • Organizer: 第55回NMR討論会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2016-11-16
• [Presentation] 光照射固体NMRによるセンサリーロドプシンIIの光反応過程における光中間体構造の解析 (ポスター) (2016)
  • Author(s): 槙野義輝、川村出、沖津貴志、和田昭盛、須藤雄気、加茂直樹、内藤晶、上田一義
  • Organizer: 第55回NMR討論会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2016-11-16
• [Presentation] 13C固体NMRを用いたDMPC脂質二重膜存在下におけるペプチドホルモングルカゴンの線維構造の解析 (ポスター) (2016)
  • Author(s): 羽矢和未、川村出、内藤晶
  • Organizer: 第55回NMR討論会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2016-11-16
• [Presentation] 固体NMRによる膜タンパク質の機能的な相互作用の観測 (ポスター) (2016)
  • Author(s): 川村 出
  • Organizer: 第4回公開シンポジウム 理論と実験の協奏による柔らかな分子系の機能の科学
  • Place of Presentation: 名古屋工業大学 ４号館ホール
  • Year and Date: 2016-10-27
• [Presentation] 固体NMRによるKR2のレチナール結合ポケットの構造解析 (ポスター) (2016)
  • Author(s): 重田安里寿、伊藤奨太、沖津貴志、和田昭盛、井上圭一、神取秀樹、川村出
  • Organizer: 第4回公開シンポジウム 理論と実験の協奏による柔らかな分子系の機能の科学
  • Place of Presentation: 名古屋工業大学 ４号館ホール
  • Year and Date: 2016-10-27
• [Presentation] Dアミノ酸含有抗菌性ペプチド ボンビニンH4による膜分断作用の31P固体NMR解析 (ポスター) (2016)
  • Author(s): 金田志穂、川村出
  • Organizer: 第4回公開シンポジウム 理論と実験の協奏による柔らかな分子系の機能の科学
  • Place of Presentation: 名古屋工業大学 ４号館ホール
  • Year and Date: 2016-10-27
• [Presentation] Structural analysis of retinal binding pocket of sodium pumping rhodopsin, KR2, studied by soild-state NMR (Oral) (2016)
  • Author(s): A. Shigeta, S. Ito, T. Okitsu, A. Wada, K. Inoue, H. Kandori, I. Kawamura
  • Organizer: EMBO conference 17th International Conference on Retinal Proteins
  • Place of Presentation: Neues Palais, Potsdam
  • Year and Date: 2016-10-02
  • Int'l Joint Research
• [Presentation] Solid-state NMR structural study of Tyr residue in the retinal binding-pocket of microbial rhodopsins. (Oral) (2016)
  • Author(s): I. Kawamura, R. Nishikawa, H. Seki, A. Shigeta, Y. Makino, T. Tsukamoto, T. Okitsu, A. Wada, Y. Sudo, A. Naito
  • Organizer: EMBO conference 17th International Conference on Retinal Proteins
  • Place of Presentation: Neues Palais, Potsdam
  • Year and Date: 2016-10-02
  • Int'l Joint Research
• [Presentation] Conformational changes in retinal of sensory rhodopsin as revealed by in-situ photo-irradiation solid-state NMR and chemical shift calculation (Poster) (2016)
  • Author(s): Y. Makino, I. Kawamura, T. Okitsu, A. Wada, Y. Sudo, N. Kamo, Y. Sudo, A. Naito, K. Ueda
  • Organizer: EMBO conference 17th International Conference on Retinal Proteins
  • Place of Presentation: Neues Palais, Potsdam
  • Year and Date: 2016-10-02
  • Int'l Joint Research
• [Presentation] Dアミノ酸を含む抗菌ペプチド ボンビニンH4の細菌模倣膜に対する相互作用の解析 (2016)
  • Author(s): 金田志穂、北橋由貴、内藤晶、川村出
  • Organizer: 第12回Dアミノ酸学術講演会
  • Place of Presentation: 高知大学朝倉キャンパス
  • Year and Date: 2016-09-14
• [Presentation] D体フェニルアラニンを含む抗菌ペプチドPhenylseptinの脂質膜との相互作用解析 (2016)
  • Author(s): 松尾雄大、川村出
  • Organizer: 第12回Dアミノ酸学術講演会
  • Place of Presentation: 高知大学朝倉キャンパス
  • Year and Date: 2016-09-14
• [Presentation] Solid-state NMR structural study of photoreceptor membrane protein (2016)
  • Author(s): I. Kawamura
  • Organizer: 第65回高分子討論会 (日韓合同セッション)
  • Place of Presentation: 神奈川大学
  • Year and Date: 2016-09-14
  • Invited
• [Presentation] Structural analysis of retinal binding pocket of sodium pumping rhodopsin, KR2, by solid-state NMR (Young Investigator Award, Poster) (2016)
  • Author(s): A. Shigeta, S. Ito, T. Okitsu, A. Wada, K. Inoue, H. Kandori, I. Kawamura
  • Organizer: XXVIIth International Conference on Magnetic Resonance in Biological Systems (ICMRBS2016)
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Analysis of interaction of antimicrobial peptides bombinin H2 and H4 with the mimetic bacterial membrane by 31P solid-state NMR (Poster) (2016)
  • Author(s): S. Kaneda, Y. Kitahashi, I. Kawamura, A. Naito
  • Organizer: XXVIIth International Conference on Magnetic Resonance in Biological Systems (ICMRBS2016)
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Structural change of Tyr185 in the vicinity of the retinal chromophore in bacteriorhodopsin by 13C solid-state NMR (Poster) (2016)
  • Author(s): Y. Otani, A. Shigeta, Y. Kebukawa, K. Kobayashi, T. Okitsu, A. Wada, S. Tuzi, A. Naito, I. Kawamura
  • Organizer: XXVIIth International Conference on Magnetic Resonance in Biological Systems (ICMRBS2016)
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Structural changes of retinal in sensory rhodopsin II as revealed by in-situ photo-irradiation solid-state NMR (Poster) (2016)
  • Author(s): Y. Makino, I. Kawamura, T. Okitsu, A. Wada, Y. Sudo, N. Kamo, A. Naito, K. Ueda
  • Organizer: XXVIIth International Conference on Magnetic Resonance in Biological Systems (ICMRBS2016)
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Structure of retinal-binding pocket of microbial rhodopsin as revealed by solid-state NMR (Poster) (2016)
  • Author(s): I. Kawamura, R. Nishikawa, H. Seki, A. Shigeta, Y. Otani, S. Nakatani, Y. Makino, T. Okitsu, A. Wada, Y. Sudo, A. Naito
  • Organizer: XXVIIth International Conference on Magnetic Resonance in Biological Systems (ICMRBS2016)
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2016-08-21
  • Invited
• [Presentation] 固体NMRの基礎 (2016)
  • Author(s): 川村出
  • Organizer: 第28回NMR講座 NMR研究会(高分子学会 )
  • Place of Presentation: 東京海洋大学
  • Invited
• [Book] Experimental Approaches of NMR Spectroscopy, Chap 9 Structure Determination of Membrane Peptides and Proteins by Solid-State NMR (2018)
  • Author(s): Izuru Kawamura, Kazushi Norisada, Akira Naito
  • Publisher: Springer
  • ISBN: 9789811059650
• [Book] Experimental Approaches of NMR Spectroscopy, Chap 5 Photoirradiation and Microwave Irradiation NMR Spectroscopy (2018)
  • Author(s): Akira Naito, Yoshiteru Makino, Yugo Tasei, Izuru Kawamura
  • Publisher: Springer
  • ISBN: 9789811059650
• [Book] 抗菌ペプチドの機能解明と技術利用 -第1章 抗菌ペプチドの構造-機能相関の研究 (2017)
  • Author(s): 川村 出
  • Publisher: シーエムシー出版
  • ISBN: 9784781312453
• [Book] Modern Magnetic Resonance (2017)
  • Author(s): Akira Naito, Yoshiteru Makino, Izuru Kawamura
  • Publisher: Springer
• [Remarks] 横浜国立大学 研究者総覧
  • URL: http://er-web.jmk.ynu.ac.jp/html/KAWAMURA_Izuru/ja.html
• [Remarks] 横浜国立大学 研究者総覧
  • URL: http://er-web.jmk.ynu.ac.jp/html/KAWAMURA_Izuru/ja.html