高速AFMで明らかにする真正細菌型イオンポンプロドプシンの多量体構造と機能動態

• Project Area: Science on Function of Soft Molecular Systems by Cooperation of Theory and Experiment
• Project/Area Number: 16H00830
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Nagoya University (2017); Kanazawa University (2016)
• Principal Investigator: 内橋 貴之 名古屋大学, 理学研究科, 教授 (30326300)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 微生物ロドプシン / 高速原子間力顕微鏡 / 一分子計測 / 多量体構造 / 構造変化 / １分子計測(SMD) / 走査プローブ顕微鏡 / 蛋白質 / 一分子計測(SMD) / １分子イメージング・ナノ計測

Outline of Annual Research Achievements

１．イオンポンプロドプシンの多量体構造の決定: 様々な微生物型ロドプシンを高速AFM法と円二色性(CD)分光法により網羅的に解析し、脂質膜に再構成された状態での多量体構造を決定した。高速AFM観察により、GR、KR2、FR、KrActR、QsActR等の真性細菌で見出された微生物型ロドプシンはリング状の5量体を形成しており、GPRは５量体と６量体が共存していることがわかった。また、センサリーロドプシンであるSRII、ハロロドプシンNpHRなどの古細菌型ロドプシンは三量体であることがわかった。一方、内向きH+ポンプであるPoXeRやセンサリーロドプシンASRは真性細菌で発見された微生物型ロドプシンであるが、アミノ酸残基の相同性からは古細菌型に分類され、実際多量体構造は三量体であった。また、CD分光で観察される三量体と五量体に特徴的なスペクトルは多量体構造に依存したレチナールの配向で説明できることもわかった。これらの結果から、微生物型プロドプシンの多量体構造は進化系統樹と深く関わっている一方、イオンポンプのイオン種や向きと相関がないことがわかった。
2．KR2の光誘起構造変化の観察の観察: 外向きNa+ポンプであるKR2の光誘起による構造変化の観察に向けて、KR2五量体の観察面の決定およい高解像観察に適した試料の調製を行った。野生型に比べて光サイクルが100以上遅いN112A変異体を高解像観察しながら、周期的な光照射を照射したが明瞭な構造変化は観察できなかった。このことから、BRとは異なり、KR2の光サイクル中には大規模な構造変化は起きていない可能性が示唆された。

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Applications of high-speed atomic force microscopy to real-time visualization of dynamic biomolecular processes (2018)
  • Author(s): Uchihashi Takayuki、Scheuring Simon
  • Journal Title: BBA General Subjects Volume: 1862 Issue: 2 Pages: 229-240
  • DOI: 10.1016/j.bbagen.2017.07.010
  • Peer Reviewed
• [Journal Article] Rate constants, processivity, and productive binding ratio of chitinase A revealed by single- molecule analysis (2018)
  • Author(s): Akihiko Nakamura, Tomoyuki Tasaki, Yasuko Okuni, Chihong Song, Kazuyoshi Murata, Toshiya Kozai, Mayu Hara, Hayuki Sugimoto, Kazushi Suzuki, Takeshi Watanabe, Takayuki Uchihashi, Hiroyuki Noji and Ryota Iino
  • Journal Title: Phys. Chem. Chem. Phys. Volume: 20 Issue: 5 Pages: 3010-3018
  • DOI: 10.1039/c7cp04606e
  • Peer Reviewed / Open Access
• [Journal Article] Conversion of functionally undefined homopentameric protein PbaA into a proteasome activator by mutational modification of its C-terminal segment conformation (2018)
  • Author(s): M. Yagi-Utsumi, A. Sikdar, T. Kozai, R. Inoue, M. Sugiyama, T. Uchihashi, H. Yagi, T. Satoh, and K. Kato
  • Journal Title: Protein Engineering, Design, and Selection Volume: 31 Issue: 1 Pages: 29-36
  • DOI: 10.1093/protein/gzx066
  • Peer Reviewed / Open Access
• [Journal Article] Dynamic clustering of dynamin-amphiphysin helices regulates membrane constriction and fission coupled with GTP hydrolysis (2018)
  • Author(s): Tetsuya Takeda, Toshiya Kozai, Huiran Yang, Daiki Ishikuro, Kaho Seyama, Yusuke Kumagai, Tadashi Abe, Hiroshi Yamada, Takayuki Uchihashi, Toshio Ando, Kohji Takei
  • Journal Title: eLife Volume: 7 Pages: 1-19
  • DOI: 10.7554/elife.30246
  • NAID: 120006510224
  • Peer Reviewed / Open Access
• [Journal Article] Negatively Charged Lipids Are Essential for Functional and Structural Switch of Human 2-Cys Peroxiredoxin II (2018)
  • Author(s): Haruyama Takamitsu、Uchihashi Takayuki、Yamada Yutaro、Kodera Noriyuki、Ando Toshio、Konno Hiroki
  • Journal Title: Journal of Molecular Biology Volume: 430 Issue: 5 Pages: 602-610
  • DOI: 10.1016/j.jmb.2017.12.020
  • Peer Reviewed / Open Access
• [Journal Article] Sweeping of adsorbed therapeutic proteins on prefillable syringe enhances subvisible particles generation (2018)
  • Author(s): Takahiro Maruno, Hiroki Watanabe, Takayuki Uchihashi, Satoru Adachi, Kunihito Arai, Taichi Sawaguchi and Susumu Uchiyama
  • Journal Title: Journal of Pharmaceutical Sciences Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Construction of a Triangle-Shaped Trimer and a Tetrahedron Using an α-Helix-Inserted Circular Permutant of Cytochrome c 555 (2018)
  • Author(s): Oda Akiya、Nagao Satoshi、Yamanaka Masaru、Ueda Ikki、Watanabe Hiroki、Uchihashi Takayuki、Shibata Naoki、Higuchi Yoshiki、Hirota Shun
  • Journal Title: Chemistry - An Asian Journal Volume: 印刷中 Issue: 8 Pages: 964-967
  • DOI: 10.1002/asia.201800252
  • NAID: 120006726655
  • Peer Reviewed
• [Journal Article] Structural properties determining low K+affinity of the selectivity filter in the TWIK1 K+channel (2018)
  • Author(s): Tsukamoto Hisao、Higashi Masahiro、Motoki Hideyoshi、Watanabe Hiroki、Ganser Christian、Nakajo Koichi、Kubo Yoshihiro、Uchihashi Takayuki、Furutani Yuji
  • Journal Title: Journal of Biological Chemistry Volume: 293 Issue: 18 Pages: 6969-6984
  • DOI: 10.1074/jbc.ra118.001817
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Insight into structural remodeling of the FlhA ring responsible for bacterial flagellar type III protein export (2018)
  • Author(s): Naoya Terahara, Yumi Inoue, Noriyuki Kodera, Yusuke V. Morimoto, Takayuki Uchihashi, Katsumi Imada, Toshio Ando, Keiichi Namba, and Thoru Minamino
  • Journal Title: Science Advances Volume: 印刷中
  • Peer Reviewed / Open Access
• [Journal Article] Translating MOF chemistry into supramolecular chemistry: soluble coordination nanofibers showing efficient photon upconversion (2018)
  • Author(s): Masanori Hosoyamada, Nobuhiro Yanai, Keisuke Okumura, Takayuki Uchihashi and Nobuo Kimizuka
  • Journal Title: ChemComm Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Quantum-dot antibody conjugation visualized at the single-molecule scale with high-speed atomic force microscopy (2018)
  • Author(s): Takayuki Umakoshi, Hikari Udaka, Takayuki Uchihashi, Toshio Ando, Miho Suzuki, Takeshi Fukuda
  • Journal Title: Colloids and Surfaces B: Biointerfaces Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Visualization of Protein Dynamics using High-Speed Atomic Force Microscopy and Image Analysis (2018)
  • Author(s): 内橋 貴之
  • Journal Title: Journal of Computer Chemistry, Japan Volume: 17 Issue: 1 Pages: 20-30
  • DOI: 10.2477/jccj.2018-0001
  • NAID: 130006528631
  • ISSN: 1347-1767, 1347-3824
  • Peer Reviewed / Open Access
• [Journal Article] High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM (2017)
  • Author(s): Keya Jakia Jannat、Inoue Daisuke、Suzuki Yuki、Kozai Toshiya、Ishikuro Daiki、Kodera Noriyuki、Uchihashi Takayuki、Kabir Arif Md. Rashedul、Endo Masayuki、Sada Kazuki、Kakugo Akira
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 6166-6166
  • DOI: 10.1038/s41598-017-06249-1
  • NAID: 120006368158
  • Peer Reviewed / Open Access
• [Journal Article] Fast Adsorption of Soft Hydrogel Microspheres on Solid Surfaces in Aqueous Solution (2017)
  • Author(s): Matsui Shusuke、Kureha Takuma、Hiroshige Seina、Shibata Mikihiro、Uchihashi Takayuki、Suzuki Daisuke
  • Journal Title: Angewandte Chemie International Edition (Communication) Volume: 56 Issue: 40 Pages: 12146-12149
  • DOI: 10.1002/anie.201705808
  • NAID: 120006377474
  • Peer Reviewed
• [Journal Article] Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis (2017)
  • Author(s): Harada Hirofumi、Onoda Akira、Uchihashi Takayuki、Watanabe Hiroki、Sunagawa Naoki、Samejima Masahiro、Igarashi Kiyohiko、Hayashi Takashi
  • Journal Title: Chemical Science Volume: 8 Issue: 9 Pages: 6561-6565
  • DOI: 10.1039/c7sc01672g
  • Peer Reviewed
• [Journal Article] Visualisation of a flexible modular structure of the ER folding-sensor enzyme UGGT (2017)
  • Author(s): Satoh Tadashi、Song Chihong、Zhu Tong、Toshimori Takayasu、Murata Kazuyoshi、Hayashi Yugo、Kamikubo Hironari、Uchihashi Takayuki、Kato Koichi
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 12142-12142
  • DOI: 10.1038/s41598-017-12283-w
  • Peer Reviewed / Open Access
• [Journal Article] Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy (2017)
  • Author(s): Shibata Mikihiro、Nishimasu Hiroshi、Kodera Noriyuki、Hirano Seiichi、Ando Toshio、Uchihashi Takayuki、Nureki Osamu
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 1430-1430
  • DOI: 10.1038/s41467-017-01466-8
  • NAID: 120006374160
  • Peer Reviewed / Open Access
• [Journal Article] High-speed atomic force microscopy imaging of live mammalian cells (2017)
  • Author(s): Mikihiro Shibata, Hiroki Watanabe, Takayuki Uchihashi, Toshio Ando, and Ryohei Yasuda
  • Journal Title: Biophysics and Physicobiology Volume: 14 Issue: 0 Pages: 127-135
  • DOI: 10.2142/biophysico.14.0_127
  • NAID: 130005997921
  • ISSN: 2189-4779
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Na+-induced structural transition of MotPS for stator assembly of the Bacillus flagellar motor (2017)
  • Author(s): Terahara Naoya、Kodera Noriyuki、Uchihashi Takayuki、Ando Toshio、Namba Keiichi、Minamino Tohru
  • Journal Title: Science Advances Volume: 3 Issue: 11 Pages: 4119-4119
  • DOI: 10.1126/sciadv.aao4119
  • Peer Reviewed / Open Access
• [Journal Article] Oriented Reconstitution of the Full-Length KcsA Potassium Channel in a Lipid Bilayer for AFM Imaging (2017)
  • Author(s): Ayumi Sumino, Takayuki Uchihashi and Shigetoshi Oiki
  • Journal Title: J. Phys. Chem. Lett. Volume: 8 Issue: 4 Pages: 785-793
  • DOI: 10.1021/acs.jpclett.6b03058
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A natural light-driven inward proton pump (2016)
  • Author(s): Keiichi Inoue, Shota Ito, Yoshitaka Kato, Yurika Nomura, Mikihiro Shibata, Takayuki Uchihashi, Satoshi P. Tsunoda and Hideki Kandori
  • Journal Title: Nature Communications Volume: 7 Issue: 1 Pages: 13415-13415
  • DOI: 10.1038/ncomms13415
  • Peer Reviewed / Open Access
• [Presentation] irect observation of self-assembly process of biological and artificial fibrils using high-speed atomic force microscopy (2018)
  • Author(s): Takayuki Uchihashi
  • Organizer: Interhierarchical understanding of materials and life through molecular observation
  • Int'l Joint Research / Invited
• [Presentation] Dynamic Observationb of Kai Proteins by HS-AFM Reveales a Mechanism of the Robustness in the Cyanobacterial Circadian Oscillator (2018)
  • Author(s): Shogo Sugiyama, Tetsuya Mori, Mark Byne, Takayuki Uchihashi, Carl H. Johnson and Toshio Ando
  • Organizer: The 62nd Annual Meeting of Biophysical Society
  • Int'l Joint Research
• [Presentation] 生命の構成部品を直接みて理解する ～ 顕微鏡技術で可視化するタンパク質のダイナミクス現象 ～ (2018)
  • Author(s): 内橋貴之
  • Organizer: 自然科学研究機構 機構長プレス懇談会 「生きているとは何か？」～みる・よむ・つくる研究領域～
  • Invited
• [Presentation] Structural Flexibility and Chaperone Activity of TClpB revealed by High-Speed AFM (2017)
  • Author(s): Takayuki Uchihashi, Yo-hei Watanabe, Ryota Iino and Toshio Ando
  • Organizer: XIX. Annual Linz Winter Workshop
  • Place of Presentation: Sommerhotel Julius-Raab-Heim, Linz, Austria
  • Year and Date: 2017-02-03
  • Int'l Joint Research / Invited
• [Presentation] Direct visualization of dynamic molecular interactions using HS-AFM (2017)
  • Author(s): Takayuki Uchihashi
  • Organizer: Frontier Bioorganization Forum 2017: Dynamical ordering and integrated functions of biomolecular systems
  • Int'l Joint Research / Invited
• [Presentation] Direct visualization of single molecule dynamics by high-speed atomic force microscopy (2017)
  • Author(s): Takayuki Uchihashi
  • Organizer: Telluride Science Research Center Workshop on Protein Dynamics
  • Int'l Joint Research / Invited
• [Presentation] Oligomeric state and conformational dynamics of eubacterial ion-pumping rhodopsin studied by high-speed AFM (2017)
  • Author(s): Takayuki Uchihashi
  • Organizer: KAKENHI International Symposium on “Studying the Function of Soft Molecular Systems”
  • Int'l Joint Research / Invited
• [Presentation] High speed atomic force microscopy for a tool to visualize dynamic events on biological systems from single molecules to living cells (2017)
  • Author(s): Takayuki Uchihashi
  • Organizer: Workshop on “Nanofluidics in Biological Systems”
  • Int'l Joint Research / Invited
• [Presentation] Direct observation of single molecule dynamics at work with high-speed atomic force microscopy (2017)
  • Author(s): Takayuki Uchihashi
  • Organizer: Frontier in Single Molecule Biophysics
  • Int'l Joint Research / Invited
• [Presentation] Visualization of Single-Molecule Dynamics Using High-Speed Atomic Force Microscopy (2017)
  • Author(s): Takayuki Uchihashi
  • Organizer: The 2nd Korea-Japan Joint Symposium on Single-Molecule Biophysics 2017
  • Int'l Joint Research / Invited
• [Presentation] High-speed atomic force microscopy: A tool for visualizing dynamic behavior from proteins to cells (2017)
  • Author(s): Takayuki Uchihashi
  • Organizer: The 28th 2017 International Symposium on Micro-NanoMechanical and Human Science
  • Int'l Joint Research / Invited
• [Presentation] In-line Force Measurements with High-speed AFM (2017)
  • Author(s): Christian Ganser, Takayuki Uchihashi
  • Organizer: The 25th International Colloquium on Scanning Probe Microscopy
  • Int'l Joint Research
• [Presentation] Two-step process for disassembly mechanism of proteasome α7 homo-tetradecamer by α6 revealed by high-speed atomic force microscopy (2017)
  • Author(s): Toshiya Kozai, Taichiro Sekiguchi, Tadashi Satoh, Hirokazu Yagi, Koichi Kato and Takayuki Uchihashi
  • Organizer: The 25th International Colloquium on Scanning Probe Microscopy
  • Int'l Joint Research
• [Presentation] Development of new mirro-tilter unit for tip-scanning high-speed atomic force microscopy (2017)
  • Author(s): Hiroki Watanabe, Takayuki Uchihashi and Shinnosuke Yamanaka and Toshio Ando
  • Organizer: The 25th International Colloquium on Scanning Probe Microscopy
  • Int'l Joint Research
• [Presentation] 高速AFMで明らかにするKaiタンパク質間の動的相互作用 (2017)
  • Author(s): 内橋貴之
  • Organizer: 第６９回 日本細胞生物学会大会, シンポジウム「分子の集合・離脱がつかさどる動的な細胞機能」
  • Invited
• [Presentation] 高速原子間力顕微鏡で可視化するタンパク質の動的秩序 (2017)
  • Author(s): 内橋貴之
  • Organizer: 第１７回 日本蛋白質科学会年会, ワークショップ「蛋白質動的秩序のマルチプローブを用いた統合的解析
  • Invited
• [Presentation] 高速AFMを用いた生体分子のその場観察 (2017)
  • Author(s): 内橋貴之
  • Organizer: ２０１７年真空・表面科学合同講演会, 合同シンポジウム「バイオ表面・界面，細胞，生体組織のオペランド計測
  • Invited
• [Presentation] 高速原子間力顕微鏡で可視化する生体・人工高分子の動態 (2017)
  • Author(s): 内橋貴之
  • Organizer: ２０１７年真空・表面科学合同講演会, 表面：プローブ顕微鏡研究部会「走査プローブ顕微鏡によるナノ表面科学の最前線」
  • Invited
• [Presentation] 高速原子間力顕微鏡による生体分子のダイナミクス計測 (2017)
  • Author(s): 内橋貴之
  • Organizer: 新世代研究所 バイオ単分子研究会「タンパク質の作動原理の理解へ向けて - 機能する姿を活写する -
  • Invited
• [Presentation] Analysis of Rotational Dynamics of Rotorless Enterococcus hirae V1-ATPase using High-Speed Atomic Force Microscopy (2017)
  • Author(s): Motonori Imamura, Kazuya Nakamoto, Shintaro Maruyama, Fumihiro Kawai, Ryota Iino, Takayuki Uchihashi, Takeshi Murata, Toshio Ando
  • Organizer: 日本生物物理学会第55回年会. シンポジウム "Analysis of Protein Dynamics in Solution"
  • Int'l Joint Research / Invited
• [Presentation] Dynamic remodeling of Dynamin complexes during membrane fission (2017)
  • Author(s): Tetsuya Takeda, Daiki Ishikuro, Huiran Yang, Toshiya Kozai, Kaho Seyama, Yusuke Kumagai, Hiroshi Yamada, Takayuki Uchihashi, Toshio Ando, Kohji Takei
  • Organizer: 日本生物物理学会第55回年会, シンポジウム "Biophysics of molecular assembly and biological membrane"
  • Int'l Joint Research / Invited
• [Presentation] High-Speed AFM Observation of Domain Flexibility Related to Enzymatic Function of CRISPR-Cas9 (2017)
  • Author(s): Takayuki Uchihashi, Mikihiro Shibata, Hiroshi Nishimasu, Noriyuki Kodera, Seiichi Hirano, Toshio Ando, Osamu Nureki
  • Organizer: 第54回日本生物物理学会年会 シンポジウム: "Softness and functions of biological molecules under various environments"
  • Int'l Joint Research / Invited
• [Presentation] 高速原子間力顕微鏡で可視化する Kai タンパク質間相互作用のダイナミクス (2017)
  • Author(s): 内橋貴之
  • Organizer: 第２４回日本時間生物学会学術大会 シンポジウム「２４時間の創出原理」
  • Invited
• [Presentation] 高速原子間力顕微鏡で可視化する生体膜反応ダイナミクス (2017)
  • Author(s): 内橋貴之
  • Organizer: 2017年度 生命科学系合同年次大会 ワークショップ「最先端の表面科学手法による生体膜反応の実動作下計測」
  • Invited
• [Presentation] Visualization of protein molecules in action by high-speed atomic force microscopy (2016)
  • Author(s): Mikihiro Shibata, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando
  • Organizer: 日本生物物理学会第５４回年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
  • Invited
• [Presentation] Direct Visualization of Single Molecule Dynamics at Work with High-Speed Atomic Force Microscopy (2016)
  • Author(s): Takayuki Uchihashi
  • Organizer: BioNANO 2016
  • Place of Presentation: Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland
  • Year and Date: 2016-11-22
  • Int'l Joint Research / Invited
• [Presentation] タンパク質のダイナミクスを可視化する高速原子間力顕微鏡 (2016)
  • Author(s): 内橋貴之
  • Organizer: 生体ボリュームイメージング研究部会＆生理研研究会合同ワークショップ「電子顕微鏡ビッグデータが拓くバイオメディカルサイエンス」～限界を超えるための顕微鏡技術～
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2016-11-17
  • Invited
• [Presentation] 高速原子間力顕微鏡で可視化する生体分子のダイナミクス (2016)
  • Author(s): 内橋貴之
  • Organizer: 理研シンポジウム: 第4回「光量子工学研究 -若手・中堅研究者から見た光量子工学の展開-」
  • Place of Presentation: 理化学研究所
  • Year and Date: 2016-11-01
  • Invited
• [Presentation] 高速AFMによる生体分子ダイナミクスのその場観察 (2016)
  • Author(s): 内橋貴之
  • Organizer: 日本物理学会2016年秋季大会 領域9シンポジウム「表面界面ナノ構造のその場観察」
  • Place of Presentation: 金沢大学角間キャンパス
  • Year and Date: 2016-09-15
  • Invited
• [Presentation] 高速AFMによる生体試料の動態イメージング (2016)
  • Author(s): 内橋貴之
  • Organizer: 第25回日本バイオイメージング学会学術集会 シンポジウム3「ナノバイオイメージング：１分子から細胞までの先端手法」
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2016-09-06
  • Invited
• [Presentation] Dynamic interaction between Kai proteins dependent on phosphorylation states of KaiC revealed by HS-AFM (2016)
  • Author(s): Takayuki Uchihashi, Tetsuya Mori, Shogo Sugiyama, Carl H. Johnson, and Toshio Ando
  • Organizer: 4th Kanazawa Bio-AFM Workshop 2016
  • Place of Presentation: KKRホテル金沢
  • Year and Date: 2016-09-03
  • Int'l Joint Research / Invited
• [Presentation] Direct Visualization of Single Molecule Dynamics by High-Speed Atomic Force Microscopy (2016)
  • Author(s): Takayuki Uchihashi, Noriyuki Kodera and Toshio Ando
  • Organizer: Gordon Research Conference, Single Molecule Approach to Biology
  • Place of Presentation: Chinese University of Hong Kong, Hong Kong
  • Year and Date: 2016-07-03
  • Int'l Joint Research / Invited
• [Presentation] Visualization of Functional Dynamics of Biological Molecules by High-Speed AFM (2016)
  • Author(s): Takayuki Uchihashi
  • Organizer: 分子研研究会 “超機能分子の創成 合成、計測、数理が織りなす社会実装分子の戦略的設計と開発”
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2016-06-27
  • Invited
• [Presentation] 高速原子間力顕微鏡で調べる回転分子モーターの構造ダイナミクス (2016)
  • Author(s): 内橋貴之
  • Organizer: 第１６回 日本蛋白質科学会年会 ワークショップ”回転分子モーターを調べてつくって理解する”
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2016-06-09
  • Invited
• [Presentation] 高速AFMによる膜タンパク質のダイナミクス観察 (2016)
  • Author(s): 内橋貴之
  • Organizer: 蛋白研セミナー ”膜タンパク質の構造ダイナミクス”
  • Place of Presentation: 大阪大学蛋白質科学研究所
  • Year and Date: 2016-05-12
  • Invited
• [Presentation] Direct Observation of Single Molecule Dynamics at Work with High-Speed Atomic Force Microscopy (2016)
  • Author(s): Takayuki Uchihashi
  • Organizer: Les Houches-TSRC Workshop on Protein Dynamics
  • Place of Presentation: cole de Physique des Houches, France
  • Year and Date: 2016-04-03
  • Int'l Joint Research / Invited
• [Book] "High-Speed Atomic Force Microcopy in "Compendium of Surface and Interface Analysis (2018)
  • Author(s): Takayuki Uchihashi
  • Publisher: Springer
  • ISBN: 9789811061554
• [Book] パリティ, 「高速原子間力顕微鏡によるタンパク質の動画撮影」 (2018)
  • Author(s): 内橋貴之
  • Publisher: 丸善出版
• [Patent(Industrial Property Rights)] 走査型プローブ顕微鏡 (2016)
  • Inventor(s): 内橋貴之，柴田幹大, 古寺哲幸
  • Industrial Property Rights Holder: 内橋貴之，柴田幹大, 古寺哲幸
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-234584
  • Filing Date: 2016-12-01
• [Patent(Industrial Property Rights)] 昇温ホルダおよびプローブ顕微鏡 (2016)
  • Inventor(s): 内橋貴之，足立彗, 古寺哲幸
  • Industrial Property Rights Holder: 内橋貴之，足立彗, 古寺哲幸
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-233494
  • Filing Date: 2016-11-30
• [Patent(Industrial Property Rights)] チャンバーアレイの製造方法 (2016)
  • Inventor(s): 古寺哲幸，豊田貴大，内橋貴之
  • Industrial Property Rights Holder: 古寺哲幸，豊田貴大，内橋貴之
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-232100
  • Filing Date: 2016-11-30