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NMDA受容体依存性LTPの制御のための光プローブの作成

Publicly Offered Research

Project AreaResonance Biology for Innovative Bioimaging
Project/Area Number 16H01438
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKyoto University (2017)
Institute of Physical and Chemical Research (2016)

Principal Investigator

林 康紀  京都大学, 医学研究科, 教授 (90466037)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywords細胞骨格 / シナプス可塑性 / 記憶固定化 / 長期記憶 / 光増感法 / コフィリン / 記憶・学習 / 樹状突起スパイン / LOVドメイン / CREB / 光プローブ / 光分子不活性化
Outline of Annual Research Achievements

Memory is retained in the hippocampus after initial learning. It is then transferred to the cortex where it is permanently stored. Although it has been speculated that LTP-like mechanism is involved in this process, there has been no information when and where it happens. We previously found that in the initial phase of LTP, cofilin is transported to the spine and persistently accumulates. Therefore, we assume that cofilin-dependent synaptic plasticity plays an important role in memory consolidation. In order to inactivate cofilin, we employed a genetically encoded photosensitizer, SuperNova. To validate this system, structural LTP was induced with two-photon (2P) uncaging of MNI-glutamate in the single dendritic spine, which expresses SN-fused cofilin (CFL-SN). Followed illumination at 10 min after LTP induction, the spine volume decreased. The decrease in the spine volume was also observed up to 30 min but not at 50 min. Furthermore, the laser irradiation on the spine 1 min before sLTP had no effect on the spine enlargement. These results indicate cofilin is critical for maintenance of LTP up to 30 min after induction, and the inactivation of cofilin had negligible effect on LTP induction and the volume of unstimulated spine. We confirmed electrically recorded LTP is canceled by recording field EPSP from stratum radiatum of CA1.

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (5 results)

All 2018 2017

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Hippocampus-Dependent Goal Localization by Head-Fixed Mice in Virtual Reality2017

    • Author(s)
      Sato Masaaki、Kawano Masako、Mizuta Kotaro、Islam Tanvir、Lee Min Goo、Hayashi Yasunori
    • Journal Title

      eneuro

      Volume: 4 Issue: 3 Pages: 1-17

    • DOI

      10.1523/eneuro.0369-16.2017

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Photomarking Relocalization Technique for Correlated Two-Photon and Electron Microcopy Imaging of Single Stimulated Synapses2017

    • Author(s)
      Bosch Miquel、Castro Jorge、Sur Mriganka、Hayashi Yasunori
    • Journal Title

      Methods Mol. Biol.

      Volume: 1538 Pages: 185-214

    • DOI

      10.1007/978-1-4939-6688-2_14

    • ISBN
      9781493966868, 9781493966882
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Hippocampus-dependent goal localization by head-fixed mice in virtual reality2017

    • Author(s)
      Masaaki Sato, Masako Kawano, Kotaro Mizuta, Tanvir Islam, Min Goo Lee, and Yasunori Hayashi
    • Journal Title

      eNeuro

      Volume: in press

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] NMDA 受容体依存性LTP の制御のための光プローブの作成 - 記憶を光で制御する -2018

    • Author(s)
      林 康紀
    • Organizer
      レゾナンスバイオ班会議
    • Related Report
      2017 Annual Research Report
  • [Presentation] NMDA 受容体依存性LTP の制御のための光プローブの作成2017

    • Author(s)
      林 康紀
    • Organizer
      レゾナンスバイオ班会議
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-26   Modified: 2022-12-28  

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