The DNA elements of vernalization insensitive 3 gene for quantitative and priming epigenetic memory of cold

• Project Area: Integrative system of autonomous environmental signal recognition and memorization for plant plasticity
• Project/Area Number: 16H01459
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Tsukuba
• Principal Investigator: ブザス ディアナ・ミハエラ 筑波大学, 生命環境系, 准教授 (00616229)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
• Keywords: epigenetics / chromatin / 遺伝子 / 植物

Outline of Annual Research Achievements

VIN3 is a chromatin modifier best characterizedfor its role in the vernalization pathway. The epigenetic regulation of VIN3 during coldalso offers a unique opportunity to study eukaryotic gene regulation in the chromatin context because multiple chromatin regulators operate at VIN3 locus. In this project, progress was made in understanding the regulatory mechanisms by chromatin modifying system Polycomb/Trithorax, depositing the repressive H3K27me3 (via action of PRC2 and or PRC1) based on the chromatin dynamics during primig by cold. Also, a candidate gene approach identified candidates upstream or downstream VIN3 in the cold priming response.

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Capturing environmental memory in DNA, with a little help from chromatin (2017)
  • Author(s): Diana Mihaela Buzas
  • Journal Title: plant and Cell Physiology Volume: 10 Issue: 8 Pages: 1302-1312
  • DOI: 10.1093/pcp/pcx092
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Emerging links between iron-sulfur clusters and 5-methylcytosine base excision repair in plants (2016)
  • Author(s): Buzas D M
  • Journal Title: Genes & Genetic Systems Volume: 91 Issue: 2 Pages: 51-62
  • DOI: 10.1266/ggs.16-00015
  • NAID: 130005421223
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] From the laboratory to the field: assaying histone methylation at <i>FLOWERING LOCUS C</i> in naturally growing <i>Arabidopsis halleri</i> (2016)
  • Author(s): Nishio H, Buzas DM, Nagano AJ, Suzuki Y, Sugano S, Ito M, Morinaga S, Kudoh H.
  • Journal Title: Genes & Genetic Systems Volume: 91 Issue: 1 Pages: 15-26
  • DOI: 10.1266/ggs.15-00071
  • NAID: 130005165463
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Open Access
• [Presentation] The VIN3 quantitative and priming memory of cold (2017)
  • Author(s): Buzas D M
  • Organizer: Environmental Stress Adaptation and Memory in Plants
  • Place of Presentation: Yokohama, Kanagawa, RIKEN
  • Year and Date: 2017-02-27
  • Int'l Joint Research / Invited
• [Presentation] Vernalization upstream and downstream of FLC: from annuals to perennials and from lab to field (2017)
  • Author(s): Diana Buzas
  • Organizer: Second meeting of the plant epigenetics consortium in Japan, NIG Mishima
  • Invited
• [Remarks]
  • URL: https://www.researchgate.net/profile/Diana_Buzas