篩部から発信される茎成長シグナルの解析

• Project Area: Integrative system of autonomous environmental signal recognition and memorization for plant plasticity
• Project/Area Number: 16H01462
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nagoya University
• Principal Investigator: 打田 直行 名古屋大学, トランスフォーマティブ生命分子研究所, 特任准教授 (40467692)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
• Keywords: ペプチドホルモン / 受容体 / 茎伸長 / 茎伸長シグナル

Outline of Annual Research Achievements

高等植物の体は様々な細胞群が協調して振舞うことで成り立つが、その全身的な協調を達成するための「長距離シグナル」の実体や作用メカニズムに関する知見は極めて少ない。特に、長距離シグナルはそもそもは局所的な細胞応答をきっかけに生まれ、その部位から周囲に向け発信されると想定されるが、その最初の段階である「シグナルの発生の仕組み」に関する知見は圧倒的に不足している。本研究では、茎全体の協調的な伸長の制御で茎の内皮細胞から分泌される機能冗長的なペプチドEPFL4とEPFL6が、それらの受容体であるERECTA（ER）によって篩部伴細胞で受容されると、篩部伴細胞から茎全体の協調した伸長を導くさらなるシグナルが発生する、という事象において、この篩部伴細胞で生まれて茎全体に作用するシグナルの実体、ならびに、このシグナル作用が及ぶ範囲や作用先が受ける影響、の解明を目指している。
本年度は、昨年度までに完成させたEPFL6シグナルをOFFからONに切り替えることができる発現誘導系と最適な発現誘導条件を用いて、EPFL6刺激をONに切り換えた際の遺伝子発現の変動を網羅的に解析するためのRNA-seqを実施した。それら遺伝子群に関して、篩部伴細胞での発現の有無、分泌シグナルの有無、ホルモン関連遺伝子かどうか、などで分類し、本事象での重要因子候補としての順位付けを行った。興味深いことに、この中には、篩部から周辺領域に遠隔作用を発揮できる可能性を持つ因子が複数含まれている。今後は、各因子の個別解析を進めていく。

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] ERECTA-family genes coordinate stem cell functions between the epidermal and internal layers of the shoot apical meristem. (2018)
  • Author(s): Kimura Y, Tasaka M, Torii KU and Uchida N
  • Journal Title: Development Volume: 145 Issue: 1
  • DOI: 10.1242/dev.156380
  • NAID: 120006473533
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair (2018)
  • Author(s): Uchida N, Takahashi K, Iwasaki R, Yamada R, Yoshimura M, Endo TA, Kimura S, Zhang H, Nomoto M, Tada Y, Kinoshita T, Itami K, Hagihara S and Torii KU
  • Journal Title: Nature Chem. Biol. Volume: 14 Issue: 3 Pages: 299-305
  • DOI: 10.1038/nchembio.2555
  • NAID: 120006473593
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 凸凹戦略によりデザインした人工オーキシンと受容体のペアにより植物のオーキシンシグナル伝達経路をハイジャックする (2018)
  • Author(s): 打田 直行、高橋 宏二、萩原 伸也、鳥居 啓子
  • Journal Title: First Author's Volume: N/A Pages: 016
  • DOI: 10.7875/first.author.2018.016
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mechanisms and strategies shaping plant peptide hormones. (2017)
  • Author(s): Hirakawa Y, Torii KU and Uchida N
  • Journal Title: Plant Cell Physiol. Volume: 8 Issue: 8 Pages: 1313-1318
  • DOI: 10.1093/pcp/pcx069
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Cryptic bioactivity capacitated by synthetic hybrid plant peptides (2017)
  • Author(s): Hirakawa Y, Shinohara H, Welke K, Irle S, Matsubayashi Y, Torii KU, Uchida N.
  • Journal Title: Nature Commun. Volume: 8 Issue: 1 Pages: 14318-14318
  • DOI: 10.1038/ncomms14318
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] ERECTA-family receptor genes prevent excessive progression of radial growth and premature initiation of fiber differentiation in the Arabidopsis hypocotyl vasculature. (2017)
  • Author(s): Ikematsu, S., Tasaka, M., Torii, K.U., and Uchida, N.
  • Journal Title: New Phytologist Volume: 213 Issue: 4 Pages: 1697-1709
  • DOI: 10.1111/nph.14335
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Stem development through vascular tissues: EPFL-ERECTA family signaling that bounces in and out of phloem (2017)
  • Author(s): Tameshige T, Ikematsu S, Torii KU and Uchida N
  • Journal Title: J. Exp. Bot. Volume: 68 Issue: 1 Pages: 45-53
  • DOI: 10.1093/jxb/erw447
  • NAID: 120006305583
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Impact of erecta mutation on leaf serration differs between Arabidopsis accessions. (2016)
  • Author(s): ameshige, T., Okamoto, S., Tasaka, M., Torii, K.U., and Uchida, N.
  • Journal Title: Plant Signal & Behav Volume: 17 Issue: 12 Pages: e1261231-e1261231
  • DOI: 10.1080/15592324.2016.1261231
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A secreted peptide and its receptors shape the auxin response patterns and leaf margin morphogenesis. (2016)
  • Author(s): Tameshige, T., Okamoto, S., Lee, J.S., Aida, M., Tasaka, M., Torii, K.U., and Uchida, N.
  • Journal Title: Current Biology Volume: 26 Issue: 18 Pages: 2478-2485
  • DOI: 10.1016/j.cub.2016.07.014
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Efficient in planta detection and dissection of de novo mutation events in the Arabidopsis thaliana disease resistance gene UNI (2016)
  • Author(s): Tomohiko Ogawa, Akiko Mori, Kadunari Igari, Miyo Terao Morita, Masao Tasaka, and Naoyuki Uchida
  • Journal Title: Plant Cell Physiol Volume: 57 Issue: 6 Pages: 1123-1132
  • DOI: 10.1093/pcp/pcw060
  • NAID: 120005895201
  • Peer Reviewed
• [Presentation] 植物ホルモン応答の自在操作に向けた合成生物学的凸凹戦略 (2018)
  • Author(s): 打田直行, 高橋宏二, 萩原伸也, 鳥居啓子
  • Organizer: 日本植物生理学会年会第59回大会
• [Presentation] A small-compound-based approach to dissect mechanisms coordinating hypocotyl growth (2018)
  • Author(s): Murao M, Kato R, Endo H, Hagihara S, Itami K, Torii KU, Uchida N
  • Organizer: 日本植物生理学会年会第59回大会
• [Presentation] Functions of ERECTA-family receptor kinases in Arabidopsis leaf vein formation (2018)
  • Author(s): Nakashima Y, Kumamoto D, Endo H, Tasaka M, Torii KU, Uchida N
  • Organizer: 日本植物生理学会年会第59回大会
• [Presentation] ERECTA-family receptors coordinate layer-specific stem cell maintenance in the shoot apical meristem (2018)
  • Author(s): Kimura Y, Tasaka M, Torii KU, Uchida N
  • Organizer: 日本植物生理学会年会第59回大会
• [Presentation] Versatile control of growth and development by ERECTA-family receptor signaling (2017)
  • Author(s): Uchida N
  • Organizer: Plant Peptides & Receptors 2017
  • Int'l Joint Research / Invited
• [Presentation] A bump-and-hole strategy toward freehand manipulation of plant hormone signaling (2017)
  • Author(s): Uchida N
  • Organizer: IoC-IPMB-ITbM Joint Symposium on New Frontiers by Fusing Chemistry and Biology
  • Int'l Joint Research / Invited
• [Presentation] 葉の周縁部におけるオーキシン応答パターンはPIN1とEPFL2 ペプチドによって制御される (2017)
  • Author(s): 爲重才覚，鳥居啓子，打田直行
  • Organizer: 日本植物生理学会年会第58回大会
  • Place of Presentation: 鹿児島
• [Presentation] A peptide-receptor system shaping leaf margins (2017)
  • Author(s): Tameshige S, Okamoto S, Lee JS, Aida M, Tasaka M, Torii KU, ○Uchida
  • Organizer: 日本植物生理学会年会第58回大会
  • Place of Presentation: 鹿児島
• [Presentation] ERECTA ファミリーは茎頂の幹細胞維持を層特異的に制御する (2017)
  • Author(s): 木村友香，田坂昌生，鳥居啓子，打田直行
  • Organizer: 日本植物生理学会年会第58回大会
  • Place of Presentation: 鹿児島
• [Presentation] Control of haploid organ size by CLE peptide signaling (2017)
  • Author(s): Yuki Hirakawa, Naoyuki Uchida, Takayuki Kohchi, Shinichiro Sawa, John Bowman
  • Organizer: 日本植物生理学会年会第58回大会
  • Place of Presentation: 鹿児島
• [Presentation] Hybrid engineering of CLE peptides (2016)
  • Author(s): Hirakawa Y, Shinohara H, Welke K, Irle S, Matsubayashi Y, Torii KU and Uchida N
  • Organizer: The Cold Spring Harbor Asia conference on Latest Advances in Plant Development & Environmental Response
  • Place of Presentation: 淡路島
  • Int'l Joint Research
• [Presentation] Layer-specific stem cell maintenance in the shoot apical meristem by ERECTA-family receptor genes (2016)
  • Author(s): Kimura Y, Tasaka M, Torii KU, Uchida N
  • Organizer: The Cold Spring Harbor Asia conference on Latest Advances in Plant Development & Environmental Response
  • Place of Presentation: 淡路島
  • Int'l Joint Research
• [Presentation] ヘテロな層構造を持つ茎頂幹細胞集団の調和したふるまいを支える仕組み (2016)
  • Author(s): 打田直行, 木村友香, 田坂昌生, 鳥居啓子
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄
• [Presentation] ハイブリッド型植物ペプチドホルモンの研究 (2016)
  • Author(s): 平川有宇樹, 篠原秀文, Kai Welke, Stephan Irle, 松林嘉克, 鳥居啓子, 打田直行
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄
• [Presentation] 植物の幹細胞維持にニッチは必須か？～茎頂の幹細胞の場合～ (2016)
  • Author(s): 打田 直行
  • Organizer: 第2回幹細胞研究会 幹細胞の基本原理と多様性 ～植物と動物の比較から～
  • Place of Presentation: 神戸
• [Presentation] A Secreted Peptide EPFL2 and Its Receptors Shape the Auxin Response Pattern in Leaf Margin (2016)
  • Author(s): Tameshige S, Okamoto S, Lee JS, Aida M, Tasaka M, Torii KU, Uchida N
  • Organizer: Auxin conference 2016
  • Place of Presentation: Sanya, China
  • Int'l Joint Research
• [Presentation] Hybrid engineering of CLE peptides (2016)
  • Author(s): Hirakawa Y, Shinohara H, Welke K, Irle S, Matsubayashi Y, Torii KU and Uchida N
  • Organizer: PLANT PEPTIDES & RECEPTORS 2016, the 4th European Workshop on Peptide Signalling in Plants
  • Place of Presentation: Bischoffsheim, France
  • Int'l Joint Research
• [Presentation] Coordination of stem cell behavior between different cell layers in the shoot apical meristem (2016)
  • Author(s): Uchida N
  • Organizer: The 27th International Conference on Arabidopsis Research
  • Place of Presentation: Gyeongju, Korea
  • Int'l Joint Research / Invited