Systematic and integrative analysis of the occurrence of genetic heterogeneity in CRC

• Project Area: Conquering cancer through neo-dimensional systems understanding
• Project/Area Number: 16H01576
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Kyushu University
• Principal Investigator: 三森 功士 九州大学, 大学病院, 教授 (50322748)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000); Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
• Keywords: fractile / 多様性 / PDX / 選択圧 / エキソームシークエンス / PDXマウスモデル / 全エキソームシークエンス / 大腸癌 / ＰＤＸ

Outline of Annual Research Achievements

われわれはこれまでの研究結果(Uchi et al. 2016 PLoS Genet.)から、我々は「腫瘍内遺伝学的不均一性は、大腸癌の成長に伴って常に生み出され続ける。その分布は全体と部分が相似している(fractal)」という仮説を立てた。本研究では、PDXマウスモデル及びLaser Micro dissection(LMD)法を駆使し、次世代シークエンサーによるエキソームシークエンスを行うことで、この仮説を検証した。1）大腸癌の症例集積とPDXマウスモデルの作成：九州大学病院別府病院外科にて手術を行った進行大腸癌症例２例において、摘出後の大腸癌原発巣組織を一部採取し、NOD/SCIDマウスの皮下に腫瘍組織を移植することでPDXマウスモデルを作成した。この際、同時に正常大腸組織の一箇所、大腸癌原発巣組織の複数箇所より組織を採取し冷凍保存した。マウスに移植された腫瘍が増大し、適切な容積となった時点でマウスを安楽死させ、腫瘍を摘出した。この腫瘍を更に別のマウスに移植することを繰り返し、経時的に腫瘍を採取した。１症例目では順調に腫瘍組織の採取を行ったが、２症例目では組織の生着が見られず、実験を中止した。2）DNA抽出と全エキソームシークエンス：1.で得られた正常大腸組織、大腸癌組織及びマウスに移植して得られた大腸癌組織計１３検体よりgenomic DNAを抽出し、全エキソームシークエンシングを行った。１３検体全てにおいてシークエンスが終了し、現在遺伝子変異について解析を終えた。その結果、継代を経る毎に多様性が失われ、収束していることを明らかにした。

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] GWAS identifies two novel colorectal cancer loci at 16q24.1 and 20q13.12. (2018)
  • Author(s): Tanikawa C, Kamatani Y, Takahashi A, Nagayama S, Mimori K, Mori M, Yasuda J, Tsuboi A, Shimizu A, Sasaki M, Yamaji T, Tsugane S, Naito M, Wakai K, Koyama T, Takezaki T, Yuji K, Murakami Y, Nakamura Y, Matsuda K, et al.
  • Journal Title: Carcinogenesis Volume: Epub Pages: 652-660
  • DOI: 10.1093/carcin/bgy026
  • Peer Reviewed / Open Access
• [Journal Article] Identification of ARL4C as a Peritoneal Dissemination-Associated Gene and Its Clinical Significance in Gastric Cancer. (2018)
  • Author(s): Hu Q, Masuda T, Sato K, Tobo T, Nambara S, Kidogami S, Hayashi N, Kuroda Y, Ito S, Eguchi H, Saeki H, Oki E, Maehara Y, Mimori K
  • Journal Title: Ann Surg Oncol Volume: 25(3) Pages: 745-753
  • DOI: 10.1245/s10434-017-6292-6
  • Peer Reviewed / Open Access
• [Journal Article] Antitumor effects of the antiparasitic agent ivermectin via inhibition of Yes-associated protein 1 expression in gastric cancer (2018)
  • Author(s): Nambara S, Masuda T, Nishio M, Kuramitsu S, Tobo T, Ogawa Y, Hu Q, Iguchi T, Kuroda Y, Ito S, Eguchi H, Sugimachi K, Saeki H, Oki E, Maehara Y, Suzuki A, Mimori K
  • Journal Title: ONCOTARGET Volume: 8(64) Pages: 107666-107677
  • DOI: 10.18632/oncotarget.22587
  • NAID: 120006454566
  • Peer Reviewed / Open Access
• [Journal Article] Combined mutation of Apc, Kras and Tgfbr2 effectively drives metastasis of intestinal cancer. (2018)
  • Author(s): Sakai E, Nakayama M, Oshima H, Kouyama Y, Niida A, Fujii S, Ochiai A, Nakayama KI, Mimori K, Suzuki Y, Hong CP, Ock CY, Kim SJ, and Oshima M.
  • Journal Title: Cancer Research Volume: 78 Pages: 1334-1346
  • DOI: 10.1158/0008-5472.can-17-3303
  • NAID: 120006452378
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Circulating exosomal microRNA-203 is associated with metastasis possibly via inducing tumor-associated macrophages in colorectal cancer. (2017)
  • Author(s): Takano Y, Masuda T, Iinuma H, Yamaguchi R, Sato K, Tobo T, Hirata H, Kuroda Y, Nambara S, Hayashi N, Iguchi T, Ito S, Eguchi H, Ochiya T, Yanaga K, Miyano S, Mimori K
  • Journal Title: Oncotarget Volume: 8(45) Pages: 78598-78613
  • Peer Reviewed / Open Access
• [Journal Article] MicroRNAs as Biomarkers in Colorectal Cancer. (2017)
  • Author(s): Masuda T, Hayashi N, Kuroda Y, Ito S, Eguchi H, Mimori K
  • Journal Title: Cancers (Basel) Volume: 9(9)
  • DOI: 10.3390/cancers9090124
  • Peer Reviewed / Open Access
• [Journal Article] Japanese genome-wide association study identifies a significant colorectal cancer susceptibility locus at chromosome 10p14 (2017)
  • Author(s): Takahashi Y, Sugimachi K, Yamamoto K, Niida A, Shimamura T, Sato T, Watanabe M, Tanaka J, Kudo S, Sugihara K, Hase K, Kusunoki M, Yamada K, Shimada Y, Moriya Y, Suzuki Y, Miyano S, Mori M, Mimori K
  • Journal Title: Cancer Sci Volume: 108(11) Pages: 2239-2247
  • DOI: 10.1111/cas.13391
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Clinical significance of ZNF750 gene expression, a novel tumor suppressor gene, in esophageal squamous cell carcinoma (2017)
  • Author(s): Nambara S, Masuda T, Tobo T, Kidogami S, Komatsu H, Sugimachi K, Saeki H, Oki E, Maehara Y,Mimori K
  • Journal Title: Oncol Lett. Volume: 14(2) Pages: 1795-1801
  • DOI: 10.3892/ol.2017.6341
  • Peer Reviewed / Open Access
• [Journal Article] Significance of CD47 expression in gastric cancer. (2017)
  • Author(s): Sudo T, Takahashi Y, Sawada G, Uchi R, Mimori K, Akagi Y
  • Journal Title: Oncol Lett. Volume: 14(1) Pages: 801-809
  • Peer Reviewed / Open Access
• [Journal Article] DDR2 Expression Is Associated with a High Frequency of Peritoneal Dissemination and Poor Prognosis in Colorectal Cancer. (2017)
  • Author(s): Sasaki S, Ueda M, Iguchi T, Kaneko M, Nakayama H, Watanabe T, Sakamoto A, Mimori K
  • Journal Title: Anticancer Res Volume: 37(5) Pages: 2587-2591
  • Peer Reviewed
• [Journal Article] Phosphoserine Phosphatase Is a Novel Prognostic Biomarker on Chromosome 7 in Colorectal Cancer (2017)
  • Author(s): Sato K, Masuda T, Hu Q, Tobo T, Kidogami S, Ogawa Y, Saito T, Nambara S, Komatsu H, Hirata H, Sakimura S, Uchi R, Hayashi N, Iguchi T, Eguchi H, Ito S, Nakagawa T, Mimori K
  • Journal Title: Anticancer Res. Volume: 37(5) Pages: 2365-2371
  • Peer Reviewed
• [Journal Article] Up-regulation of SLC9A9 Promotes Cancer Progression and Is Involved in Poor Prognosis in Colorectal Cancer. (2017)
  • Author(s): Ueda M, Iguchi T, Masuda T, Komatsu H, Nambara S, Sakimura S, Hirata H, Uchi R, Eguchi H, Ito S, Sugimachi K, Mizushima T, Doki Y, Mori M, Mimori K
  • Journal Title: Anticancer Res. Volume: 37(5) Pages: 2255-2263
  • Peer Reviewed
• [Journal Article] phyC: Clustering cancer evolutionary trees (2017)
  • Author(s): Matsui Y, Niida A, Uchi R, Mimori K, Miyano S, Shimamura T:
  • Journal Title: PLoS Comput Biol Volume: 13(5) Pages: 1005509-1005509
  • DOI: 10.1371/journal.pcbi.1005509
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Overexpression of heat-shock factor 1 is associated with a poor prognosis in esophageal squamous cell carcinoma (2017)
  • Author(s): Tsukao Y, Yamasaki M, Miyazaki Y, Makino T, Takahashi T, Kurokawa Y, Miyata H, Nakajima K, Takiguchi S, Mimori K, Mori M, Doki Y:
  • Journal Title: Oncol Lett. Volume: 13(3) Pages: 1819-1825
  • DOI: 10.3892/ol.2017.5637
  • Peer Reviewed / Open Access
• [Journal Article] The loss of CASP4 expression is associated with poor prognosis in esophageal squamous cell carcinoma. (2017)
  • Author(s): Shibamoto M, Hirata H, Eguchi H, Sawada G, Sakai N, Kajiyama Y, Mimori K
  • Journal Title: Oncol Lett Volume: 13 Pages: 1761-1766
  • DOI: 10.3892/ol.2017.5646
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Altered Expression of Hippo Signaling Pathway Molecules in Intrahepatic Cholangiocarcinoma. (2017)
  • Author(s): Sugimachi K, Nishio M, Aishima S, Kuroda Y, Iguchi T, Komatsu H, Hirata H, Sakimura S, Eguchi H, Bekki Y, Takenaka K, Maehara Y, Suzuki A, Mimori K.
  • Journal Title: Oncology. Volume: - Pages: 67-74
  • DOI: 10.1159/000463390
  • Peer Reviewed / Open Access
• [Journal Article] Extraction of cell-free DNA from urine, using polylysine-coated silica particles. (2017)
  • Author(s): Takano S, Hu Q, Amamoto T, Refinetti P, Mimori K, Funatsu T, Kato M
  • Journal Title: Anal Bioanal Chem Volume: 印刷中 Pages: 4021-4025
  • DOI: 10.1007/s00216-017-0345-3
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] MOB1-YAP1/TAZ-NKX2.1 axis controls bronchioalveolar cell differentiation, adhesion and tumour formation (2017)
  • Author(s): Otsubo K, Goto H, Nishio M, Kawamura K, Yanagi S, Nishie W, Sasaki T, Maehama T, Nishina H, Mimori K, Nakano T, Shimizu H, Mak TW, Nakao K, Nakanishi Y, Suzuki A
  • Journal Title: Oncogene. Volume: 印刷中 Pages: 4201-4211
  • DOI: 10.1038/onc.2017.58
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Clinical Significance of FANCD2 Gene Expression and its Association with Tumor Progression in Hepatocellular Carcinoma (2017)
  • Author(s): Komatsu H, Masuda T, Iguchi T, Nambara S, Sato K, Hu Q, Hirata H, Ito S, Eguchi H, Sugimachi K, Eguchi H, Doki Y, Mori M, Mimori K
  • Journal Title: Anticancer Res. Volume: 37(3) Pages: 1083-1090
  • Peer Reviewed
• [Journal Article] Clinical Impact of Tumor-Infiltrating Lymphocytes in Esophageal Squamous Cell Carcinoma (2017)
  • Author(s): Sudo T, Nishida R, Kawahara A, Saisho K, Mimori K, Yamada A, Mizoguchi A, Kadoya K, Matono S, Mori N, Tanaka T, Akagi Y.
  • Journal Title: Annals of Surgical Oncology Volume: in press Pages: 3763-3770
  • DOI: 10.1245/s10434-017-5796-4
  • Peer Reviewed / Open Access
• [Journal Article] Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development (2017)
  • Author(s): Iwaya T, Sawada G, Amano S, Kume K, Ito C, Endo F, Konosu M, Shioi Y, Akiyama Y, Takahara T, Otsuka K, Nitta H, Koeda K, Mizuno M, Nishizuka S, Sasaki A, Mimori K.
  • Journal Title: Int J Oncol Volume: 50(2) Pages: 441-447
  • DOI: 10.3892/ijo.2016.3817
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Overexpression of CXCR7 Is a Novel Prognostic Indicator in Gastric Cancer (2017)
  • Author(s): Nambara S, Iguchi T, Oki E, Tan P, Maehara Y, Mimori K.
  • Journal Title: Dig Surg. Volume: Epub Pages: 312-318
  • DOI: 10.1159/000452977
  • Peer Reviewed / Open Access
• [Journal Article] The Expression of CCAT2, a Novel Long Noncoding RNA Transcript, and rs6983267 Single-Nucleotide Polymorphism Genotypes in Colorectal Cancers (2017)
  • Author(s): Kasagi Y, Oki E, Ando K, Ito S, Iguchi T, Sugiyama M, Nakashima Y, Ohgaki K, Saeki H, Mimori K, Maehara Y:
  • Journal Title: Oncology Volume: 92(1) Pages: 48-54
  • DOI: 10.1159/000452143
  • Peer Reviewed / Open Access
• [Journal Article] Attenuated RND1 Expression Confers Malignant Phenotype and Predicts Poor Prognosis in Hepatocellular Carcinoma (2017)
  • Author(s): Komatsu H, Iguchi T, Masuda T, Hirata H, Ueda M, Kidogami S, Ogawa Y, Sato K, Hu Q, Nambara S, Saito T, Sakimura S, Uchi R, Ito S, Eguchi H, Sugimachi K, Eguchi H, Doki Y, Mori M, Mimori K.
  • Journal Title: Ann Surg Oncol. Volume: 24(3) Pages: 850-859
  • DOI: 10.1245/s10434-016-5573-9
  • Peer Reviewed / Open Access
• [Journal Article] As a Novel Prognostic Marker, Cysteine/histidine-rich 1 (CYHR1) is a Therapeutic Target in Patients with Esophageal Squamous Cell Carcinoma (2017)
  • Author(s): Desaki R, Sawada G, Okumura H, Ikeda R, Tanabe K, Komatsu H, Mimori K, Mori M, Kita Y, Uchikado Y, Arigami T, Uenosono Y, Owaki T, Ishigami S, Natsugoe S.
  • Journal Title: Ann Surg Oncol Volume: Epub Pages: 586-593
  • DOI: 10.1245/s10434-015-5031-0
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Clinical Significance of FANCD2 Gene Expression and its Association with Tumor Progression in Hepatocellular Carcinoma. (2017)
  • Author(s): Komatsu H, Masuda T, Iguchi T, Nambara S, Sato K, Hu Q, Hirata H, Ito S, Eguchi H, Sugimachi K, Eguchi H, Doki Y, Mori M, Mimori K.
  • Journal Title: Anticancer Res Volume: 37(3) Pages: 1083-1090
  • Peer Reviewed / Open Access
• [Journal Article] miR-146a Polymorphism (rs2910164) Predicts Colorectal Cancer Patients' Susceptibility to Liver Metastasis. Iguchi T, Nambara S, Masuda T, Komatsu H, Ueda M, Kidogami S, Ogawa Y, Hu Q, Sato K, Saito T, Hirata H, Sakimura S, Uchi R, Hayashi N, Ito S, Eguchi H, Sugimachi K, Maehara Y, Mimori K. (2016)
  • Author(s): Iguchi T, Nambara S, Masuda T, Komatsu H, Ueda M, Kidogami S, Ogawa Y, Hu Q, Sato K, Saito T, Hirata H, Sakimura S, Uchi R, Hayashi N, Ito S, Eguchi H, Sugimachi K, Maehara Y, Mimori K.
  • Journal Title: PLoS One. Volume: 11(11)
  • DOI: 10.1371/journal.pone.0165912
  • Peer Reviewed / Open Access
• [Journal Article] Somatic mutations in plasma cell-free DNA are diagnostic markers for esophageal squamous cell carcinoma recurrence. (2016)
  • Author(s): Ueda M, Iguchi T, Masuda T, Nakahara Y, Hirata H, Uchi R, Niida A, Momose K, Sakimura S, Chiba K, Eguchi H, Ito S, Sugimachi K, Yamasaki M, Suzuki Y, Miyano S, Doki Y, Mori M, Mimori K
  • Journal Title: Oncotarget. Volume: 7(38) Pages: 62280-62291
  • DOI: 10.18632/oncotarget.11409
  • Peer Reviewed / Open Access
• [Journal Article] 8q24 Polymorphisms and Diabetes Mellitus Regulate Apolipoprotein A-IV in Colorectal Carcinogenesis. (2016)
  • Author(s): Sugimachi K, Yamaguchi R, Eguchi H, Ueda M, Niida A, Sakimura S, Hirata H, Uchi R, Shinden Y, Iguchi T, Morita K, Yamamoto K, Miyano S, Mori M, Maehara Y, Mimori K
  • Journal Title: Ann Surg Oncol. Volume: 23(Suppl 4) Pages: 546-551
  • DOI: 10.1245/s10434-016-5374-1
  • Peer Reviewed / Open Access
• [Journal Article] Expression of PD-L1 and HLA Class I in esophageal squamous cell carcinoma: Prognostic factors for patient outcome. (2016)
  • Author(s): Ito S, Okano S, Morita M, Saeki H, Tsutsumi S, Tsukihara H, Nakashima Y, Ando K, Imamura Y, Ohgaki K, Oki E, Kitao H, Mimori K, Maehara Y.
  • Journal Title: Ann Surg Oncol Volume: 23(Suppl 4) Pages: 508-515
  • DOI: 10.1245/s10434-016-5376-z
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] HOXB7 Expression is a Novel Biomarker for Long-term Prognosis After Resection of Hepatocellular Carcinoma. (2016)
  • Author(s): Komatsu H, Iguchi T, Masuda T, Ueda M, Kidogami S, Ogawa Y, Nambara S, Sato K, Hu Q, Saito T, Hirata H, Sakimura S, Uchi R, Hayashi N, Ito S, Eguchi H, Sugimachi K, Eguchi H, Doki Y, Mori M, Mimori K.
  • Journal Title: Anticancer Res. Volume: 36(6) Pages: 2767-73
  • Peer Reviewed / Open Access
• [Journal Article] Aberrant Methylation of FOXE1 Contributes to a Poor Prognosis for Patients with Colorectal Cancer (2016)
  • Author(s): Sugimachi K, Matsumura T, Shimamura T, Hirata H, Uchi R, Ueda M, Sakimura S, Iguchi T, Eguchi H, Masuda T, Morita K, Takenaka K, Maehara Y, Mori M, Mimori K.
  • Journal Title: Annals of Surgical Oncology Volume: 23(12) Pages: 3948-3955
  • DOI: 10.1245/s10434-016-5289-x
  • Peer Reviewed / Open Access
• [Journal Article] Decreased Expression of Fructose-1,6-bisphosphatase Associates with Glucose Metabolism and Tumor Progression in Hepatocellular Carcinoma. (2016)
  • Author(s): Hirata H, Sugimachi K, Komatsu H, Ueda M, Masuda T, Uchi R, Sakimura S, Nambara S, Saito T, Shinden Y, Iguchi T, Eguchi H, Ito S, Terashima K, Sakamoto K, Hirakawa M, Honda H, Mimori K.
  • Journal Title: Cancer Res Volume: 76(11) Pages: 3265-7
  • DOI: 10.1158/0008-5472.can-15-2601
  • Peer Reviewed / Open Access
• [Journal Article] Increased Copy Number of the Gene Encoding SF3B4 Indicates Poor Prognosis in Hepatocellular Carcinoma. (2016)
  • Author(s): Iguchi T, Komatsu H, Masuda T, Nambara S, Kidogami S, Ogawa Y, Hu Q, Saito T, Hirata H, Sakimura S, Uchi R, Hayashi N, Ito S, Eguchi H, Sugimachi K, Maehara Y, Mimori K.
  • Journal Title: Anticancer Res. Volume: 36(5) Pages: 2139-44
  • Peer Reviewed / Open Access
• [Journal Article] Integrated molecular profiling of human gastric cancer identifies DDR2 as a potential regulator of peritoneal dissemination (2016)
  • Author(s): Kurashige J, Hasegawa T, Niida A, Sugimachi K, Deng N, Mima K, Uchi R, Sawada G, Takahashi Y, Eguchi H, Inomata M, Kitano S, Fukagawa T, Sasako M, Sasaki H, Sasaki S, Mori M, Yanagihara K, Baba H, Miyano S, Tan P, Mimori K.
  • Journal Title: Sci Rep Volume: 6 Pages: 22371-22371
  • DOI: 10.1038/srep22371
  • NAID: 120006352400
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Clinical and biological significance of circulating tumor cells in cancer (2016)
  • Author(s): Masuda T, Hayashi N, Iguchi T, Ito S, Eguchi H, Mimori K.
  • Journal Title: Mol Oncol Volume: 10 Pages: 408-17
  • DOI: 10.1016/j.molonc.2016.01.010
  • Peer Reviewed / Open Access
• [Journal Article] Clinical and biological significance of transcription termination factor, RNA polymerase I in human liver hepatocellular carcinoma. (2016)
  • Author(s): Komatsu H, Iguchi T, Ueda M, Nambara S, Saito T, Hirata H, Sakimura S1, Takano Y1, Uchi R1, Shinden Y, Eguchi H, Masuda T, Sugimachi K, Eguchi H, Doki Y, Mori M, Mimori K.
  • Journal Title: Oncol Rep. Volume: 35 Pages: 2073-80
  • DOI: 10.3892/or.2016.4593
  • Peer Reviewed / Open Access
• [Presentation] 大腸がんにおける進化機構のシフトによる腫瘍内多様性の創出 (2018)
  • Author(s): 三森功士
  • Organizer: 第14回日本消化管学会総会学術集会
• [Presentation] 大腸がんにおける新たな進化と選択圧について (2018)
  • Author(s): 三森功士
  • Organizer: 第2回消化器外科臨床応用をめざす研究交流会
  • Invited
• [Presentation] 大腸がんにおける新たな進化と多様性創出機構について (2017)
  • Author(s): 三森功士
  • Organizer: Translation Research Seminar
• [Presentation] がん転移再発の早期診断におけるctDNAの臨床的有用性について (2017)
  • Author(s): 三森功士
  • Organizer: JBICバイオ関連基盤技術研究会
• [Presentation] 薬物動態から見直す大腸がん化学療法 (2017)
  • Author(s): 三森功士
  • Organizer: 第28回日本消化器癌発生学会
  • Invited
• [Presentation] 消化器がん転移再発診断と治療評価法に関するctDNA解析の臨床的有用性と今後の課題 (2017)
  • Author(s): 三森功士
  • Organizer: 第55回日本癌治療学会学術集会
• [Presentation] 食道がんゲノム情報に基づく診断と治療戦略の構築 (2017)
  • Author(s): 三森功士
  • Organizer: JDDW2017
• [Presentation] 大腸発がんから進行がんにいたる過程のゲノムレベル進化について (2017)
  • Author(s): 三森功士
  • Organizer: 第3回がんゲノム・エピゲノムセミナー
• [Presentation] ctDNAにおいて突然変異の検出が可能な原発巣の特徴 (2017)
  • Author(s): 三森功士
  • Organizer: 第76回日本癌学会学術総会
• [Presentation] 消化器がん症例の様々な臨床的局面における血中ctDNA突然変異検出の意義 (2017)
  • Author(s): 三森功士
  • Organizer: 第26回日本がん転移学会
• [Presentation] 消化器癌におけるctDNA検出の臨床的意義と臨床応用にむけての提言 (2017)
  • Author(s): 三森功士
  • Organizer: 第72回日本消化器外科学会
• [Presentation] プロパゲルマニウム（SK-818）の骨髄由来細胞制御機構による癌転移巣増殖制御について (2017)
  • Author(s): 三森功士
  • Organizer: 第38回日本炎症・再生医学会
  • Invited
• [Presentation] 大腸がんの進化と転移再発を決める選択圧について (2017)
  • Author(s): 三森功士
  • Organizer: 昭和大学横浜北部病院2017年消化器センター軽井沢セミナー
  • Invited
• [Presentation] がん治療における革新的変化を踏まえた最先端技術，新たな開発戦略の現状と今後の方向性 (2017)
  • Author(s): 三森功士
  • Organizer: 第23回抗悪性腫瘍薬開発フォーラム,
• [Presentation] 大腸癌のゲノム進化にみる自然選択説と中立進化説～固形がん原発巣の一腫瘍内多様性について～ (2017)
  • Author(s): 三森功士
  • Organizer: 九州胆・膵癌治療フォーラム
  • Place of Presentation: 福岡
• [Presentation] 大腸がんのゲノム解析による新たな発がん・がん進展モデルの提唱 (2017)
  • Author(s): 三森功士
  • Organizer: 第26回泌尿器科分子・細胞研究会
  • Place of Presentation: 大分
  • Invited
• [Presentation] PSPHは7番染色体上に存在する大腸癌の新規ドライバー候補遺伝子である (2016)
  • Author(s): 佐藤晋彰、胡慶江、木戸上真也、齋藤衆子、南原翔、小松久晃、平田秀成、﨑村正太郎、黒田陽介、伊藤修平、江口英利、増田隆明、三森功士
  • Organizer: 第75回日本癌学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2016-10-06
• [Presentation] がん幹細胞および幹細胞ニッチを制御する分子標的治療の確立を目指して (2016)
  • Author(s): 三森功士
  • Organizer: 第14回日本臨床腫瘍学会
  • Place of Presentation: 神戸
  • Year and Date: 2016-07-28
  • Invited
• [Presentation] 大腸癌におけるmiR-146a多型（rs2910164）の臨床的意義. (2016)
  • Author(s): 井口友宏、増田隆明、小松久晃、南原翔、木戸上真也、胡慶江、林直樹、伊藤修平、江口英利、三森功士
  • Organizer: 第71回日本消化器外科学会
  • Place of Presentation: 徳島
  • Year and Date: 2016-07-20
• [Presentation] 大腸癌における7番染色体上のドライバー遺伝子の同定. (2016)
  • Author(s): 江口英利、増田隆明、胡慶江、木戸上真也、南原翔、小松久晃、林直樹、井口友宏、伊藤修平、三森功士
  • Organizer: 第71回日本消化器外科学会
  • Place of Presentation: 徳島
  • Year and Date: 2016-07-20
• [Presentation] 数理生物統合解析による大腸癌のドライバー遺伝子BZW2の同定とその臨床学的意義. (2016)
  • Author(s): 増田隆明、胡慶江、木戸上真也、南原翔、小松久晃、林直樹、井口友宏、伊藤修平、江口英利、三森功士
  • Organizer: 第71回日本消化器外科学会
  • Place of Presentation: 徳島
  • Year and Date: 2016-07-20
• [Presentation] Proposal of new tactics to overcome resistance to molecular target therapy provoked heterogenetiy. (2016)
  • Author(s): 三森功士、高橋佑典、内龍太郎、新井田厚司、宮野悟、森正樹
  • Organizer: 第71回日本消化器外科学会
  • Place of Presentation: 徳島
  • Year and Date: 2016-07-20
• [Presentation] 大腸癌症例の原発巣および肝転移巣癌細胞の統合的解析による多様性の解明 (2016)
  • Author(s): 江口英利、高橋佑典,木戸上真也、胡慶江、南原翔、小松久晃、上田正射、林直樹、井口友宏、増田隆明、伊藤修平、三森功士
  • Organizer: 第116回日本外科学会
  • Place of Presentation: 大阪
  • Year and Date: 2016-04-14