Radiogenomic diagnosis of brain tumors using artificial inteligence

• Project Area: Multidisciplinary computational anatomy and its application to highly intelligent diagnosis and therapy
• Project/Area Number: 17H05308
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Osaka University
• Principal Investigator: 木下 学 大阪大学, 医学系研究科, 講師 (40448064)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 神経膠腫 / Radiomics / 深層学習 / 人工知能 / 脳腫瘍 / MRI / 分子診断技術

Outline of Annual Research Achievements

神経膠腫は中枢神経系に発生する希少がんで、手術・放射線治療・化学療法を組み合わせた集学的治療が実施されているが、予後不良な疾患である。神経膠腫は分子生物学的には数種類の異なった機序で発症していることが明らかになってきており、分子生物学的手法を用いた新たな病態理解・診断・治療のアプローチが求められている。
腫瘍の分子情報の取得には腫瘍組織の採取が必須であり、脳腫瘍の場合には開頭術という侵襲的な手技を経由する必要がある。このような侵襲的な手技を回避すべく、radiogenomicsもしくは人工知能という新規技術を発展させて、放射線画像という非侵襲的な診断技術で腫瘍の分子情報の取得を試みようとするのが本研究の主たる目的である。
本研究ではCNNによる人工知能を用いたMRIの画像特徴量抽出を行うため、自然画像からの画像特徴量抽出に優れているCNNである、AlexNetを上記解析システムに組み入れた。予備検討から、正常脳組織と脳腫瘍組織の識別においてAlexNetの第5層(conv5)まで畳み込まれた画像特徴が最も有用であることがわかった。
次にAlexNetから得られる第5層(conv5)の画像特徴量を用いて、IDH変異およびpTERT変異の推定アルゴリズム開発を行った。AlexNet-conv5の情報を付加しSupport vector machine(SVM)で解析した。その結果、従来法と比較してAlexNetから得られた画像特徴量を元データとすると10％の精度向上を確認することができた。
本研究結果では、AlexNetの神経膠腫画像特徴の抽出における有用性が証明された。

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] T₂-FLAIR Mismatch Sign Is Caused by Long T₁ and T₂ of <i>IDH</i>-mutant, 1p19q Non-codeleted Astrocytoma (2021)
  • Author(s): Kinoshita Manabu、Uchikoshi Masato、Sakai Mio、Kanemura Yonehiro、Kishima Haruhiko、Nakanishi Katsuyuki
  • Journal Title: Magnetic Resonance in Medical Sciences Volume: 20 Issue: 1 Pages: 119-123
  • DOI: 10.2463/mrms.bc.2019-0196
  • NAID: 130007993615
  • ISSN: 1347-3182, 1880-2206
  • Peer Reviewed / Open Access
• [Journal Article] Prediction of IDH and TERT promoter mutations in low-grade glioma from magnetic resonance images using a convolutional neural network (2019)
  • Author(s): Fukuma Ryohei、Yanagisawa Takufumi、Kinoshita Manabu、他
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 0-0
  • DOI: 10.1038/s41598-019-56767-3
  • Peer Reviewed / Open Access
• [Journal Article] Radiomics and MGMT promoter methylation for prognostication of newly diagnosed glioblastoma (2019)
  • Author(s): Sasaki Takahiro、Kinoshita Manabu、他
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 14435-14435
  • DOI: 10.1038/s41598-019-50849-y
  • Peer Reviewed / Open Access
• [Journal Article] MicroRNA regulating stanniocalcin-1 is a metastasis and dissemination promoting factor in glioblastoma (2019)
  • Author(s): Sakata Junichi、Sasayama Takashi、Tanaka Kazuhiro、Nagashima Hiroaki、Nakada Mitsutoshi、Tanaka Hirotomo、Hashimoto Naoya、Kagawa Naoki、Kinoshita Manabu、Nakamizo Satoshi、Maeyama Masahiro、Nishihara Masamitsu、Hosoda Kohkichi、Kohmura Eiji
  • Journal Title: Journal of Neuro-Oncology Volume: 142 Issue: 2 Pages: 241-251
  • DOI: 10.1007/s11060-019-03113-2
  • Peer Reviewed / Open Access
• [Journal Article] Practical procedures for the integrated diagnosis of astrocytic and oligodendroglial tumors (2019)
  • Author(s): Sonoda Yukihiko、The committee for molecular diagnosis of the Japan Society of Brain Tumor Pathology、Yokoo Hideaki、Tanaka Shinya、Kinoshita Manabu、Nakada Mitsutoshi、Nishihara Hiroshi
  • Journal Title: Brain Tumor Pathology Volume: in press Issue: 2 Pages: 56-62
  • DOI: 10.1007/s10014-019-00337-y
  • Peer Reviewed
• [Journal Article] Relationship between normalized distributional pattern and functional outcome in patients with acute cardiogenic cerebral embolism (2019)
  • Author(s): Takagaki Masatoshi、Kinoshita Manabu、Kawaguchi Atsushi、Murasawa Akira、Nakao Kazutami、Nakamura Hajime、Kishima Haruhiko
  • Journal Title: PLOS ONE Volume: 14 Issue: 1 Pages: 0210709-0210709
  • DOI: 10.1371/journal.pone.0210709
  • Peer Reviewed / Open Access
• [Journal Article] 11C-methionine-18F-FDG dual-PET-tracer-based target delineation of malignant glioma: evaluation of its geometrical and clinical features for planning radiation therapy. (2018)
  • Author(s): Hirata T, Kinoshita M, Tamari, Seo Y, Suzuki O, Wakai N, Achiha T, Umehara T, Arita H, Kagawa N, Kanemura Y, Shimosegawa E, Hashimoto N, Hatazawa J, Kishima H, Teshima T, Ogawa K.
  • Journal Title: Journal of Neurosurgery Volume: 21 Issue: 3 Pages: 1-11
  • DOI: 10.3171/2018.4.jns1859
  • Peer Reviewed / Open Access
• [Journal Article] Carotid artery stenting for patients with occipital?vertebral anastomosis (2018)
  • Author(s): Kajikawa Ryuichiro、Fujinaka Toshiyuki、Nakamura Hajime、Kinoshita Manabu、Nishida Takeo、Kishima Haruhiko
  • Journal Title: Interventional Neuroradiology Volume: 25 Issue: 2 Pages: 212-218
  • DOI: 10.1177/1591019918802924
  • Peer Reviewed
• [Journal Article] Characteristics and outcomes of elderly patients with diffuse gliomas: a multi-institutional cohort study by Kansai Molecular Diagnosis Network for CNS Tumors (2018)
  • Author(s): Sasaki Takahiro、Fukai Junya、Kodama Yoshinori、et al
  • Journal Title: Journal of Neuro-Oncology Volume: 140 Issue: 2 Pages: 329-339
  • DOI: 10.1007/s11060-018-2957-7
  • Peer Reviewed / Open Access
• [Journal Article] Lesion location implemented magnetic resonance imaging radiomics for predicting IDH and TERT promoter mutations in grade II/III gliomas (2018)
  • Author(s): Arita Hideyuki、Kinoshita Manabu、Kawaguchi Atsushi、Takahashi Masamichi、Narita Yoshitaka、Terakawa Yuzo、Tsuyuguchi Naohiro、Okita Yoshiko、Nonaka Masahiro、Moriuchi Shusuke、Takagaki Masatoshi、Fujimoto Yasunori、et al.
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 11773-11773
  • DOI: 10.1038/s41598-018-30273-4
  • Peer Reviewed / Open Access
• [Journal Article] Stereotactic image?based histological analysis reveals a correlation between 11C?methionine uptake and MGMT promoter methylation in non?enhancing gliomas (2018)
  • Author(s): Okita Yoshiko、Shofuda Tomoko、Kanematsu Daisuke、Yoshioka Ema、Kodama Yoshinori、Mano Masayuki、Kinoshita Manabu、Nonaka Masahiro、Nakajima Shin、Fujinaka Toshiyuki、Kanemura Yonehiro
  • Journal Title: Oncology Letters Volume: NA Pages: 1924-1930
  • DOI: 10.3892/ol.2018.8866
  • Peer Reviewed / Open Access
• [Journal Article] Effects of surgery with salvage stereotactic radiosurgery versus surgery with whole-brain radiation therapy in patients with one to four brain metastases (JCOG0504): A Phase III, noninferiority, randomized controlled trial (2018)
  • Author(s): Kayama Takamasa、Sato Shinya、Sakurada Kaori、et al
  • Journal Title: Journal of Clinical Oncology Volume: 36 Issue: 33 Pages: 3282
  • DOI: 10.1200/jco.2018.78.6186
  • Peer Reviewed / Open Access
• [Journal Article] Enlargement of papillary glioneuronal tumor in an adult after a follow-up period of 10 years: a case report (2018)
  • Author(s): Fujita Yuya、Kinoshita Manabu、Ozaki Tomohiko、Kitamura Masanori、Nakatsuka Shin-ichi、Kanemura Yonehiro、Kishima Haruhiko
  • Journal Title: Journal of Surgical Case Reports Volume: 2018 Issue: 6
  • DOI: 10.1093/jscr/rjy123
  • Peer Reviewed
• [Journal Article] Coil and Single-Stent Placement for Ruptured Dissecting Aneurysm of Middle Cerebral Artery: A Case Report (2018)
  • Author(s): Ozaki Tomohiko、Nishida Takeo、Fujita Yuya、Kishima Haruhiko、Kinoshita Manabu
  • Journal Title: World Neurosurgery Volume: 113 Pages: 208-211
  • DOI: 10.1016/j.wneu.2018.02.117
  • Peer Reviewed
• [Journal Article] Influence of region-of-interest designs on quantitative measurement of multimodal imaging of MR non-enhancing gliomas (2018)
  • Author(s): Takano Koji、Kinoshita Manabu、Arita Hideyuki、Okita Yoshiko、Chiba Yasuyoshi、Kagawa Naoki、Watanabe Yoshiyuki、Shimosegawa Eku、Hatazawa Jun、Hashimoto Naoya、Fujimoto Yasunori、Kishima Haruhiko
  • Journal Title: Oncology Letters Volume: 15 Pages: 7934-7940
  • DOI: 10.3892/ol.2018.8319
  • Peer Reviewed / Open Access
• [Journal Article] Voxel-based lesion mapping of meningioma: a comprehensive lesion location mapping of 260 lesions (2017)
  • Author(s): Hirayama Ryuichi、Kinoshita Manabu、Arita Hideyuki、Kagawa Naoki、Kishima Haruhiko、Hashimoto Naoya、Fujimoto Yasunori、Yoshimine Toshiki
  • Journal Title: Journal of Neurosurgery Volume: ― Issue: 6 Pages: 1-6
  • DOI: 10.3171/2017.3.jns17169
  • Peer Reviewed / Open Access
• [Journal Article] Preservation of Motor Function After Resection of Lower-Grade Glioma at the Precentral Gyrus and Prediction by Presurgical Functional Magnetic Resonance Imaging and Magnetoencephalography (2017)
  • Author(s): Izutsu Nobuyuki、Kinoshita Manabu、Yanagisawa Takufumi、Nakanishi Katsuhiko、Sakai Mio、Kishima Haruhiko
  • Journal Title: World Neurosurg. Volume: 107 Pages: 1045.e5-1045.e8
  • DOI: 10.1016/j.wneu.2017.07.152
  • Peer Reviewed
• [Journal Article] Intracranial stenting for nilotinib treatment-associated cerebrovascular stenosis in chronic myeloid leukemia (2017)
  • Author(s): Ozaki Tomohiko、Nakamura Hajime、Izutsu Nobuyuki、Masaie Hiroaki、Ishikawa Jun、Kinoshita Manabu
  • Journal Title: Interv Neuroradiol Volume: 23 Issue: 5 Pages: 527-530
  • DOI: 10.1177/1591019917710810
  • Peer Reviewed
• [Journal Article] Diagnostic Accuracy of Neuroimaging to Delineate Diffuse Gliomas within the Brain: A Meta-Analysis. (2017)
  • Author(s): Verburg N, Hoefnagels FWA, Barkhof F, Boellaard R, Goldman S, Guo J, Heimans JJ, Hoekstra OS, Jain R, Kinoshita M, Pouwels PJW, Price SJ, Reijneveld JC, Stadlbauer A, Vandertop WP, Wesseling P, Zwinderman AH, De Witt Hamer PC.
  • Journal Title: American Journal of Neuroradiology Volume: in press Issue: 10 Pages: 1884-1891
  • DOI: 10.3174/ajnr.a5368
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Voxel-Based Lesion Mapping of Cryptogenic Stroke in Patients with Advanced Cancer: A Detailed Magnetic Resonance Imaging Analysis of Distribution Pattern (2017)
  • Author(s): Achiha T, Takagaki M, Oe H, Sakai M, Matsui H, Nakanishi K, Ozaki T, Fujimoto Y, Yoshimine T, Nakanishi K, Kinoshita M
  • Journal Title: J Stroke Cerebrovasc Dis Volume: Epub ahead of print Issue: 7 Pages: 1521-1527
  • DOI: 10.1016/j.jstrokecerebrovasdis.2017.02.038
  • Peer Reviewed
• [Presentation] 悪性神経膠腫の「増悪」判定の意味するものとは? (2020)
  • Author(s): 木下 学
  • Organizer: 第39回医用画像学会
  • Invited
• [Presentation] グリオーマの画像診断と遺伝子解析・病理 (2020)
  • Author(s): 木下 学
  • Organizer: 第43回日本脳神経CI学会総会
  • Invited
• [Presentation] Radiomicsの展開と 大規模臨床データの利用 (2020)
  • Author(s): 木下 学
  • Organizer: 第9回 画像医療システム産業研究会
• [Presentation] 膠芽腫のRadiogenomics (2019)
  • Author(s): 木下 学
  • Organizer: 第39回日本脳神経外科コングレス総会
  • Invited
• [Presentation] Radiomics in Glioma (2019)
  • Author(s): 木下 学
  • Organizer: 第78回日本医学放射線学会総会
  • Invited
• [Presentation] Imapact of 11C-methionine/FDG dural tracer PET-based, compared with MRI-based target delination of malignant gliomas for radiation planning. (2018)
  • Author(s): Kinoshita M, Hirata T, Tamari K, Seo Y, Suzuki O, Wakai N, Achiha T, Umehara T, Arita H, Kagawa N, Kanemura Y, Shimosegawa E, Hashimoto N, Hatazawa J, Kishima H, Teshima T, Ogawa
  • Organizer: ociety for Neuro-onclogy (SNO) meeting
  • Int'l Joint Research
• [Presentation] Radiogenomics of 154 WHO grade 2 and 3 gliomas via machine learning and the impact of texture analysis. (2018)
  • Author(s): Kinoshita M, Arita H, Kawaguchi A, Takahashi M, Narita Y, Terakawa Y, Tsuyuguchi N, Okita Y, Nonaka M, Moriuchi S, Fukai J, Izumoto S, Ishibashi K, Kodama Y, Mori K, Ichimura K, Kanemura Y
  • Organizer: International Society for Magnetic Resonance in Medicine (ISMRM) 26th Scientific Meeting 2018
  • Int'l Joint Research
• [Presentation] Radionomic analysis of WHO grade 2 and 3 gliomas with genetic subgroup prediction. (2017)
  • Author(s): Kinoshita M, Arita H, Takahashi M, Narita Y, Terakawa Y, Tsuyuguchi N, Okita Y, Nonaka M, Moriuchi S, Fukai J, Izumoto S, Ishibashi K, Kodama Y, Mori K, Ichimura K, Kanemura Y
  • Organizer: Society for Neuro-onclogy (SNO) meeting.
  • Int'l Joint Research