多孔性蛋白質結晶内の異方的な金属配位設計と機能化

• Project Area: Coordination Asymmetry: Design of Asymmetric Coordination Sphere and Anisotropic Assembly for the Creation of Functional Molecules
• Project/Area Number: 17H05358
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: 上野 隆史 東京工業大学, 生命理工学院, 教授 (70332179)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Project Status: Discontinued (Fiscal Year 2018)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: タンパク質結晶 / タンパク質集合体 / 超分子化学 / タンパク質工学 / 錯体化学

Outline of Annual Research Achievements

本申請では、蛋白質の持つ異方的な配位場と、蛋白質結晶の材料としての特性に着目し、「多孔性蛋白質結晶内の金属錯体機能制御」を目指す。具体的には、(1)多孔性蛋白質結晶への金属配位場設計、(2) 金属配位による蛋白質結晶内細孔の機能化から、(3) 蛋白質結晶内細孔の動的複合機能制御を達成する。申請者がこれまで進めてきた金属化合物と蛋白質結晶の複合化技術を基軸に（JSTさきがけ研究、最先端・次世代研究開発支援プログラム）、蛋白質結晶を金属錯体の新しい反応場として利用する試みは、従来の生物無機化学や錯体化学の可能性を拡げる新しい配位制御技術としても、バイオから材料化学まで分野を超えたインパクトを与えると考える。本研究では、細胞内で自発的に極めて安定な結晶を形成する「多角体」の蛋白質結晶を用いて金属配位による機能化を進めた。特に、今年度は細胞内で形成された多孔性蛋白質結晶へ、細胞内で直接金属イオンと反応させることによる金属配位を達成し、金属錯体の集積可能な反応場を確認した。

Research Progress Status

30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

30年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] モナッシュ大学(オーストラリア)
• [Journal Article] Photoinduced in Vivo Magnetic Resonance Imaging (MRI) with Rapid CO Release from an MnCO-Protein Needle Composite (2018)
  • Author(s): Hyodo Fuminori、Sho Takeya、Maity Basudev、Fujita Kenta、Tachibana Yoko、Akashi Satoko、Mano Megumi、Hishikawa Yuki、Matsuo Masayuki、Ueno Takafumi
  • Journal Title: Chemistry - A European Journal Volume: 24 Issue: 45 Pages: 11578-11583
  • DOI: 10.1002/chem.201802445
  • Peer Reviewed
• [Journal Article] Raman spectroscopy insight into Norovirus encapsulation in Bombyx mori cypovirus cubic microcrystals (2018)
  • Author(s): Mori Hajime、Oda Naoki、Abe Satoshi、Ueno Takafumi、Zhu Wenliang、Pernstich Chris、Pezzotti Giuseppe
  • Journal Title: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Volume: 203 Pages: 19-30
  • DOI: 10.1016/j.saa.2018.05.066
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Supramolecular protein cages constructed from a crystalline protein matrix (2018)
  • Author(s): Negishi Hashiru、Abe Satoshi、Yamashita Keitaro、Hirata Kunio、Niwase Kento、Boudes Marion、Coulibaly Fasseli、Mori Hajime、Ueno Takafumi
  • Journal Title: Chemical Communications Volume: 54 Issue: 16 Pages: 1988-1991
  • DOI: 10.1039/c7cc08689j
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Photocatalytic hydrogen evolution systems constructed in cross-linked porous protein crystals (2018)
  • Author(s): Tabe Hiroyasu、Takahashi Hikaru、Shimoi Takuya、Abe Satoshi、Ueno Takafumi、Yamada Yusuke
  • Journal Title: Applied Catalysis B: Environmental Volume: 印刷中 Pages: 1124-1129
  • DOI: 10.1016/j.apcatb.2018.01.046
  • NAID: 120006939381
  • Peer Reviewed
• [Journal Article] Engineering of protein assemblies within cells (2018)
  • Author(s): Nguyen Tien K、Ueno Takafumi
  • Journal Title: Current Opinion in Structural Biology Volume: 51 Pages: 1-8
  • DOI: 10.1016/j.sbi.2017.12.005
  • Peer Reviewed
• [Journal Article] Functionalization of protein crystals with metal ions, complexes and nanoparticles (2018)
  • Author(s): Abe Satoshi、Maity Basudev、Ueno Takafumi
  • Journal Title: Current Opinion in Chemical Biology Volume: 43 Pages: 68-76
  • DOI: 10.1016/j.cbpa.2017.11.015
  • Peer Reviewed
• [Journal Article] Structure of in cell protein crystals containing organometallic complexes (2018)
  • Author(s): Abe Satoshi、Atsumi Kohei、Yamashita Keitaro、Hirata Kunio、Mori Hajime、Ueno Takafumi
  • Journal Title: Physical Chemistry Chemical Physics Volume: 20 Issue: 5 Pages: 2986-2989
  • DOI: 10.1039/c7cp06651a
  • Peer Reviewed
• [Journal Article] Artificial bio-nanomachines based on protein needles derived from bacteriophage T4 (2017)
  • Author(s): Inaba Hiroshi、Ueno Takafumi
  • Journal Title: Biophysical Reviews Volume: 10 Issue: 2 Pages: 641-658
  • DOI: 10.1007/s12551-017-0336-9
  • Peer Reviewed
• [Journal Article] Improved efficiency of nanoneedle insertion by modification with a cell-puncturing protein (2017)
  • Author(s): Ryu Seunghwan、Matsumoto Yuta、Matsumoto Takahiro、Ueno Takafumi、Silberberg Yaron R.、Nakamura Chikashi
  • Journal Title: Japanese Journal of Applied Physics Volume: 57 Issue: 3S2 Pages: 03EB02-03EB02
  • DOI: 10.7567/jjap.57.03eb02
  • NAID: 210000148743
  • Peer Reviewed
• [Presentation] Construction of Protein Needle and the Application for In Vivo MRI imaging (2018)
  • Author(s): Takafumi Ueno
  • Organizer: KAIST-Tokyo Tech Bio Joint Workshop
  • Int'l Joint Research / Invited
• [Presentation] Design of Protein Assembly for Supramolecular materials (2018)
  • Author(s): Takafumi Ueno
  • Organizer: Post-Mini workshop of Tokyo Tech Life Science and Technology International Symposium, “New Frontiers of Supramolecular Chemistry in Non-equilibrium Systems
  • Int'l Joint Research / Invited
• [Presentation] DESIGN OF PROTEIN ASSEMBLY FOR BIOINORGNIC MATERIALS (2017)
  • Author(s): Takafumi Ueno
  • Organizer: 11th Japan-China Joint Symposium on Metal Cluster Compounds
  • Int'l Joint Research / Invited
• [Presentation] Design of Bioinorganic Materials Constructed by Coordination Chemistry (2017)
  • Author(s): Takafumi Ueno
  • Organizer: 6th Asian Conference on Coordination Chemistry
  • Int'l Joint Research / Invited
• [Presentation] Functional Design of Protein Cage for Sustainable Bionanomaterial (2017)
  • Author(s): Takafumi Ueno
  • Organizer: ASPIRE FORUM 2017
  • Int'l Joint Research / Invited
• [Presentation] Design of Protein Assemblies as Molecular Templates (2017)
  • Author(s): Takafumi Ueno
  • Organizer: 10th China-Japan Joint Symposium on Functional Supramolecular Architectures
  • Int'l Joint Research / Invited
• [Presentation] Design of Protein Assemblies as Supramolecular Platforms (2017)
  • Author(s): Takafumi Ueno
  • Organizer: Frontier Bioorganization Forum 2017, “Dynamical ordering and integrated functions of biomolecular systems
  • Int'l Joint Research / Invited
• [Presentation] CO Releasing Bioinorganic Nanomaterials for CO Gas Biology (2017)
  • Author(s): Takafumi Ueno
  • Organizer: The International Advanced Drug Delivery Symposium 2017
  • Int'l Joint Research / Invited
• [Remarks] The Ueno Lab
  • URL: http://www.ueno.bio.titech.ac.jp/publication.html
• [Remarks] The Ueno Lab
  • URL: http://www.ueno.bio.titech.ac.jp/