記憶学習において作動する神経回路の遷移

• Project Area: Mechanisms underlying the functional shift of brain neural circuitry for behavioral adaptation
• Project/Area Number: 17H05545
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University
• Principal Investigator: 谷本 拓 東北大学, 生命科学研究科, 教授 (70714955)
• Project Period (FY): 2017-06-30 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2018: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000); Fiscal Year 2017: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
• Keywords: 神経情報処理

Outline of Annual Research Achievements

ショウジョウバエの匂い連合学習では、記憶の獲得・保持・読み出しの過程を明確に区別でき、その全ての過程がキノコ体と呼ばれる単一の脳構造で処理される。本研究では、記憶の素過程を定義するキノコ体出力神経の機能パターンと、その遷移を理解することを目的とする。研究代表者らの研究グループは、キノコ体の出力を担う21種類の出力神経を全て同定した。つまりキノコ体は、全ての神経種が遺伝学的に標識でき、機能操作と行動出力との因果関係を明らかにすることができる独自のモデル回路である。本研究ではこの遺伝学的リソースを用い、キノコ体の出力を構造と機能の両面から解析する。
2018年度は、キノコ体の異なる記憶の素過程における出力パターンを調べるために、嗅覚報酬・嗅覚罰記憶の各素過程における各出力神経の必要性を定量した。全ての出力神経について、その神経伝達を各素過程特異的に阻害し、記憶学習実験を行った。各出力神経の記憶に対する阻害効果から、各素過程での貢献度を定量することで、キノコ体からの出力コードが時間とともに変化する様子を明らかにした。さらに、各素過程の機能コードを実際の顕微鏡画像上にプロットし、その出力先、放出される神経伝達物質を可視化した。各出力神経細胞種の形態的特徴と記憶の各素過程の機能コードを統合することにより、例えば報酬記憶の獲得に共通する神経回路モチーフを同定した。

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Janelia Research Campus(米国)
• [Int'l Joint Research] University of Munich/University of Konstanz(ドイツ)
• [Int'l Joint Research] Janelia Research Campus(米国)
• [Int'l Joint Research] University of Munich/University of Konstanz(ドイツ)
• [Journal Article] Quantification of Aggregation and Associated Brain Areas in Drosophila Melanogaster. (2019)
  • Author(s): Okuno T, Hashimoto K, Tanimoto H.
  • Journal Title: IEEE Explore Volume: in press
  • Peer Reviewed
• [Journal Article] Courtship behavior induced by appetitive olfactory memory. (2019)
  • Author(s): Onodera Y, Ichikawa R, Terao K, Tanimoto H, Yamagata N.
  • Journal Title: J Neurogenet. Volume: 2019 Apr 8 Issue: 2 Pages: 1-9
  • DOI: 10.1080/01677063.2019.1593978
  • Peer Reviewed / Open Access
• [Journal Article] Data-driven analysis of motor activity implicates 5-HT2A neurons in backward locomotion of larval Drosophila (2018)
  • Author(s): Park Jeonghyuk、Kondo Shu、Tanimoto Hiromu、Kohsaka Hiroshi、Nose Akinao
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 1-15
  • DOI: 10.1038/s41598-018-28680-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Behavioral modulation by spontaneous activity of dopamine neurons. (2017)
  • Author(s): Toshiharu Ichinose, Hiromu Tanimoto, Nobuhiro Yamagata
  • Journal Title: Frontiers Systems Neuroscience Volume: 11 Pages: 88-88
  • DOI: 10.3389/fnsys.2017.00088
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Role of the Gustatory System in the Coordination of Feeding (2017)
  • Author(s): Vladimiros Thoma, Kimiko Kobayashi, Hiromu Tanimoto
  • Journal Title: eNeuro Volume: 4(6) Issue: 6 Pages: 0324-17
  • DOI: 10.1523/eneuro.0324-17.2017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Quantification of Aggregation and Associated Brain Areas in Drosophila Melanogaster. (2019)
  • Author(s): Okuno T, Hashimoto K, Tanimoto H.
  • Organizer: IEEE International Conference on Pervasive Computing and Communications 2019
  • Int'l Joint Research
• [Presentation] Consolidation of appetitive long-term memory through dopamine-Raf signaling. (2019)
  • Author(s): Sun H, Nishioka T, Ichinose T, Kaibuchi K, Tanimoto H.
  • Organizer: Asia Pacific Drosophila Neuroscience Conference 2019
  • Int'l Joint Research
• [Presentation] Neural circuits determining the predictive value of an odor. (2018)
  • Author(s): Yamagata N, Tanimoto H.
  • Organizer: The 17th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
  • Int'l Joint Research / Invited
• [Presentation] Cell-type specific visualization of endogenous dopamine receptors. (2018)
  • Author(s): Hiramatsu S, Takahashi T, Kondo S, Katow H, Yamagata N, Tanimoto H.
  • Organizer: 13th Japanese Drosophila Research Conference
• [Presentation] Determination of reward values through the regulation of dopamine neurons. (2018)
  • Author(s): Tanimoto H, Yamagata N.
  • Organizer: The 41st Annual Meeting of the Japan Neuroscience Society
• [Presentation] Determination of reward values by regulation of dopamine neurons. (2017)
  • Author(s): Tanimoto H.
  • Organizer: The Inaugural Asia-Pacific Drosophila Neurobiology Conference
  • Int'l Joint Research / Invited
• [Presentation] State dependent feeding control by a nutrient signal. (2017)
  • Author(s): Yamagata N, Takahashi T, Kondo S, Imanishi Y, Tanimoto H.
  • Organizer: The 39th Annual Meeting of The Japanese Society for Comparative Physiology and Biochemistry
• [Presentation] Segmentation of dopamine signalling in the fly brain. (2017)
  • Author(s): Tanimoto H.
  • Organizer: The 44th Naito Conference "Decision Making in the brain - Motivation, Prediction, and Learning"
  • Int'l Joint Research / Invited
• [Presentation] Classification of the direction and behavior of fruit flies by machine vision and image-based CNN. (2017)
  • Author(s): Okuno T, Ikarashi M, Sirigrivatanawong P, Hashimoto K, Tanimoto H.
  • Organizer: Neuro Informatics 2017
  • Int'l Joint Research
• [Presentation] Segmentation of dopamine signaling in the fly brain. (2017)
  • Author(s): Tanimoto H.
  • Organizer: Neuroscience Program of Academia Sinica Symposium on Drosophila Neurobiology
  • Int'l Joint Research / Invited
• [Presentation] Memory by dopamine signals in the mushroom body. (2017)
  • Author(s): Tanimoto H.
  • Organizer: The EMBO-kavli meeting "Neural circuits and behaviour of Drosophila"
  • Int'l Joint Research / Invited
• [Presentation] Molecules functioning downstream of dopamine signaling for olfactory associative learning in the mushroom body. (2017)
  • Author(s): Sun H, Ichinose T, Yamagata N, Abe A, Tanimoto H.
  • Organizer: The 4th Asia-Pacific Drosophila Research Conference
  • Int'l Joint Research