神経回路の選択的可視化と操作を実現するウイルスベクターシステムの開発

• Project Area: Mechanisms underlying the functional shift of brain neural circuitry for behavioral adaptation
• Project/Area Number: 17H05565
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: 井上 謙一 京都大学, 霊長類研究所, 助教 (90455395)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 神経科学 / 脳・神経 / バイオテクノロジー / ウイルスベクター / 霊長類 / オプトジェネティクス

Outline of Annual Research Achievements

本研究では、新規に開発する逆行性感染型ウイルスベクターや順行性感染型ウイルスベクターを利用して、特定の神経回路を構成するニューロン集団の選択的可視化や活動操作をおこなう新規手法を開発し、霊長類において神経回路の機能シフト研究をおこなうための基盤技術を確立するためのシステム開発研究を行っている。今年度は、ジャンクションが最適化されたキメラエンベロープタンパク質を利用したNeuRet型逆行性感染型レンチウイルスベクター(FuG-E)とHiRetベクターであるFuG-B2型の霊長類（マカク・マーモセット）およびげっし類（ラット）における感染動態や注入部位における免疫反応などの差異を線条体注入系において解析した結果、FuG-E型ベクターがどの種・どの投射系においてもFuG-B2型と同等以上の逆行性感染効率を示し、またFuG-B2型が注入部位において組織損傷と免疫誘導を引き起こすのに対し、FuG-E型は引き起こさないことを報告した(Tanabe et al., 2019 Sci Rep)。また、同様の解析を皮質注入系においても実施し、論文を投稿準備中である。一方、アデノ随伴ウイルスベクターのキャプシド改変を行い、霊長類において高い神経細胞選択性と外来遺伝子発現能を両立するウイルスベクターの開発に成功し、霊長類における化学遺伝学、光遺伝学、および光イメージングに適用した（論文投稿準備中）。さらに、独自に開発した狂犬病ウイルスベクターのゲノム改変によって作成した、ゲノム複製能や外来遺伝子発現量が異なる複数の感染伝播能欠損型ベクターの性状解析を行い、低細胞毒性型、長期発現型の伝播能欠損型狂犬病ウイルスベクターの開発を進めた。

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] NIH(米国)
• [Int'l Joint Research] KU Leuen(ベルギー)
• [Int'l Joint Research] NEI(NIH)/NIMH(NIH)(米国)
• [Int'l Joint Research] フライブルグ大学(ドイツ)
• [Journal Article] Enhancement of the transduction efficiency of a lentiviral vector for neuron-specific retrograde gene delivery through the point mutation of fusion glycoprotein type E. (2019)
  • Author(s): Kato S; Sugawara M; Kobayashi K; Kimura K; Inoue K; Takada M; Kobayashi K
  • Journal Title: J Neurosci Methods Volume: 311 Pages: 147-155
  • DOI: 10.1016/j.jneumeth.2018.10.023
  • Peer Reviewed
• [Journal Article] A note on retrograde gene transfer efficiency and inflammatory response of lentiviral vectors pseudotyped with FuG-E vs. FuG-B2 glycoproteins. (2019)
  • Author(s): Tanabe S; Uezono S; Tsuge H; Fujiwara M; Miwa M; Kato S; Nakamura K; Kobayashi K; Inoue K; Takada M
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 3567-3567
  • DOI: 10.1038/s41598-019-39535-1
  • NAID: 120006818701
  • Peer Reviewed / Open Access
• [Journal Article] Segmentation of the rabies virus genome (2018)
  • Author(s): Hidaka Yuya、Lim Chang-Kweng、Takayama-Ito Mutsuyo、Park Chun-Ho、Kimitsuki Kazunori、Shiwa Nozomi、Inoue Ken-ichi、Itou Takuya
  • Journal Title: Virus Research Volume: 252 Pages: 68-75
  • DOI: 10.1016/j.virusres.2018.05.017
  • Peer Reviewed / Open Access
• [Journal Article] Causal Role of Neural Signals Transmitted From the Frontal Eye Field to the Superior Colliculus in Saccade Generation (2018)
  • Author(s): Matsumoto Masayuki、Inoue Ken-ichi、Takada Masahiko
  • Journal Title: Frontiers in Neural Circuits Volume: 12 Pages: 69-69
  • DOI: 10.3389/fncir.2018.00069
  • NAID: 120006773079
  • Peer Reviewed / Open Access
• [Journal Article] Recruitment of calbindin into nigral dopamine neurons protects against drug-induced parkinsonism. Mov Disord [Epub ahead of print] doi: 10.1002/mds.107 (2018)
  • Author(s): Inoue K, Miyachi S, Nishi K, Okado H, Nagai Y, Minamimoto T, Nambu A, Takada M
  • Journal Title: Mov Disord Volume: － Issue: 2 Pages: 200-209
  • DOI: 10.1002/mds.107
  • Peer Reviewed
• [Journal Article] Alterations in the reduced pteridine contents in the cerebrospinal fluids of LRRK2 mutation carriers and patients with Parkinson's disease. (2018)
  • Author(s): Ichinose H; Inoue K; Arakawa S; Watanabe Y; Kurosaki H; Koshiba S; Hustad E; Takada M; Aasly JO
  • Journal Title: J Neural Transm Volume: 125 Issue: 1 Pages: 45-52
  • DOI: 10.1007/s00702-017-1784-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Multisynaptic projections from the amygdala to the ventral premotor cortex in macaque monkeys: Anatomical substrate for feeding behavior. (2018)
  • Author(s): Ishida H; Inoue K; Takada M
  • Journal Title: Front Neuroanat Volume: 12 Pages: 3-3
  • DOI: 10.3389/fnana.2018.00003
  • Peer Reviewed / Open Access
• [Journal Article] Visuomotor signals for reaching movements in the rostro-dorsal sector of the monkey thalamic reticular nucleus. (2017)
  • Author(s): Saga Y, Nakayama Y, Inoue K, Yamagata T, Hashimoto M, Tremblay L, Takada M, Hoshi E
  • Journal Title: Eur J Neurosci Volume: 印刷中 Issue: 9 Pages: 1186-1199
  • DOI: 10.1111/ejn.13421
  • NAID: 120006305932
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The use of an optimized chimeric envelope glycoprotein enhances the efficiency of retrograde gene transfer of a pseudotyped lentiviral vector in the primate brain. (2017)
  • Author(s): Tanabe S, Inoue K, Tsuge H, Uezono S, Nagaya K, Fijiwara M, Kato S, Kobayashi K, Takada M
  • Journal Title: Neurosci Res Volume: 印刷中 Pages: 45-52
  • DOI: 10.1016/j.neures.2017.02.007
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] High-speed and scalable whole-brain imaging in rodents and primates (2017)
  • Author(s): Seiriki K, Kasai A, Hashimoto T, Schulze W, Niu M, Yamaguchi S, Nakazawa T, Inoue K, Uezono S, Takada M, Naka Y, Igarashi H, Tanuma M, Waschek JA, Ago Y, Tanaka KF, Hayata-Takano A, Nagayasu K, Shintani N, Hashimoto R, Kunii Y, Hino M, Matsumoto J, Yabe H, Nagai T, Fujita K, Matsuda T, Takuma K, Baba A, Hashimoto H
  • Journal Title: Neuron Volume: 94 Issue: 6 Pages: 1085-1100
  • DOI: 10.1016/j.neuron.2017.05.017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pseudotyped lentiviral vectors for retrograde gene delivery into target brain regions. (2017)
  • Author(s): Kobayashi K; Inoue K; Tanabe S; Kato S; Takada M; Kobayashi K
  • Journal Title: Front Neuroanat Volume: 11 Pages: 65-65
  • DOI: 10.3389/fnana.2017.00065
  • Peer Reviewed / Open Access
• [Journal Article] Nonhuman primate optogenetics: Recent advances and future directions. (2017)
  • Author(s): Galvan A; Stauffer WR; Ackerson L; El-Shamayleh Y; Inoue K; Ohayon S; Schmid M.
  • Journal Title: J Neurosci Volume: 37 Issue: 45 Pages: 10894-10903
  • DOI: 10.1523/jneurosci.1839-17.2017
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 融合糖タンパク質E型変異体を用いた神経特異的逆行性ウイルスベクターによるマーモセット脳内への遺伝子導入効率の向上. (2019)
  • Author(s): 菅原正晃、加藤成樹、木村 慧、井上 謙一、高田 昌彦、小林 和人
  • Organizer: 第8回日本マーモセット研究会大会
• [Presentation] Retrograde gene transfer efficacy and inflammatory response of HiRet vs. NeuRet lentiviral vectors in the motor cortical input system of nonhuman primates and rodents. (2019)
  • Author(s): Otsuka Y; Tsuge H; Uezono S; Tanabe S; Fujiwara M; Miwa M; Kato S; Nakamura K; Kobayashi K; Inoue K; Takada M
  • Organizer: 第8回日本マーモセット研究会大会
• [Presentation] Retrograde gene transfer efficacy and inflammatory response of lentiviral vectors pseudotyped with FuG-E vs. FuG-B2 glycoproteins in the striatal input system of primates and rodents. (2019)
  • Author(s): Tanabe S; Uezono S; Tsuge H; Fujiwara M; Nagaya K; Nagaya N; Sugawara M; Miwa M; Konoike N; Kato S; Nakamura K; Kobayashi K; Inoue K; Takada M
  • Organizer: 第8回日本マーモセット研究会大会
• [Presentation] ウイルスベクターを利用した霊長類における神経ネットワーク操作 (2019)
  • Author(s): 井上 謙一
  • Organizer: 玉川大学総合人間科学ワークショップ
  • Int'l Joint Research / Invited
• [Presentation] Deep brain stimulation of the ventral midbrain facilitates the output to forelimb muscles via the primary motor cortex in monkeys. (2019)
  • Author(s): Suzuki M; Inoue K; Nakagawa H; Isa T; Takada M; Nishimura Y
  • Organizer: 3rd International Brain Stimulation Conference
  • Int'l Joint Research
• [Presentation] 霊長類脳への神経細胞特異的かつ高効率な遺伝子導入を実現する改変AAVベクターの開発. (2019)
  • Author(s): 木村 慧、永井 裕司、田辺 創思、Zheng Andi、Oh Jungmin、藤原 真紀、中野 真由子、南本 敬史、井上 謙一、高田 昌彦
  • Organizer: 第8回 生理研-霊長研-新潟脳研 合同シンポジウム
• [Presentation] Pathway-selective optogenetics for elucidating neural network function in primates (2019)
  • Author(s): Inoue K
  • Organizer: DFG-AMED joint Workshop New Direction in Systems Neuroscience
  • Int'l Joint Research / Invited
• [Presentation] Neuron-specific efficient gene transduction in the primate brain with modified AAV vectors. (2018)
  • Author(s): Kimura K; Tanabe S; Fujiwara M; Nakano M; Nagai Y; Minamimoto T; Inoue K; Takada M
  • Organizer: 第41回日本神経科学大会
• [Presentation] Lentiviral vectors pseudotyped with FuG-E and FuG-E2 glycoprotein suitable for retrograde gene transfer into neural networks involving cerebral cortical areas in nonhuman primates and rodents. (2018)
  • Author(s): Otsuka Y; Tsuge H; Uezono S; Tanabe S; Fujiwara M; Miwa M; Kato S; Nakamura K; Kobayashi K; Inoue K; Takada M
  • Organizer: 第41回日本神経科学大会
• [Presentation] Using PET imaging to monitor chemogenetic manipulation of nigrostriatal dopamine system in common marmoset. (2018)
  • Author(s): Mimura K; Nagai Y; Inoue K; Suhara T; Takada M; Minamimoto T
  • Organizer: 第41回日本神経科学大会
• [Presentation] PET imaging of selective control of neural activity with a novel DREADD agonist. (2018)
  • Author(s): Nagai Y; Miyakawa N; Huang X; Slocum S; Ono M; Inoue K; Yan X; Liu J; English J; Shimojo M; Mimura K; Hirabayashi T; Bin J; Kumata K; Higuchi M; Zhang M; Takada M; Jin J; Roth B; Minamimoto T
  • Organizer: 第41回日本神経科学大会
• [Presentation] Enhanced gene transfer efficiency of neuron-specific retrograde lentiviral vector with variants of fusion glycoprotein type E into the mouse and common marmoset brains. (2018)
  • Author(s): Sugawara M; Kato S; Kimuta K; Inoue K; Takada M; Kobayashi K
  • Organizer: 第41回日本神経科学大会
• [Presentation] Pathway-selective optogenetic modulation of value-biased saccade circuits in the monkey basal ganglia. (2018)
  • Author(s): Amita H; Hikosaka O; Hyoung F.K; Inoue K; Takada M
  • Organizer: 第41回日本神経科学大会
• [Presentation] Development of viral vectors for delivering functional molecules into nonhuman primate brains. (2018)
  • Author(s): Inoue K
  • Organizer: 第41回日本神経科学大会
  • Invited
• [Presentation] Microarchitecture of binocular disparity processing in V2: combined use of intrinsic optical imaging and 2-photon Ca2+ imaging. (2018)
  • Author(s): Fang Y; Hatanaka G; Inagaki M; Inoue K; Takada M; Fujita I
  • Organizer: International Workshop Monitoring and manipulating brain function in non-human primates
  • Int'l Joint Research
• [Presentation] High-resolution imaging of primate brains using FAST. (2018)
  • Author(s): Hashimoto H; Seiriki K; Kasai A; Nakazawa T; Inoue K; Takada M
  • Organizer: International Workshop Monitoring and manipulating brain function in non-human primates
  • Int'l Joint Research
• [Presentation] Using PET imaging to monitor chemogenetic manipulation of nigrostriatal dopamine system in common marmoset. (2018)
  • Author(s): Mimura K; Nagai Y; Inoue K; Matsumoto J; Suhara T; Nishijo H; Takada M; Minamimoto T
  • Organizer: International Workshop Monitoring and manipulating brain function in non-human primates
  • Int'l Joint Research
• [Presentation] Neuron-specific efficient gene transduction in the primate brain with Morphological modified AAV vectors. (2018)
  • Author(s): Kimura K; Tanabe S; Fujiwara M; Nagai Y; Minamimoto T; Inoue K; Takada M
  • Organizer: International Workshop Monitoring and manipulating brain function in non-human primates
  • Int'l Joint Research
• [Presentation] Differences in retrograde gene transfer efficiency and cytotoxicity between lentiviral vectors pseudotyped with FuG-E; FuG-E2; and FuG-B2 glycoprotein in primate brains. (2018)
  • Author(s): Otsuka Y; Tanabe S; Tsuge H; Uezono S; Fujiwara M; Miwa M; Kato S; Nakamura K; Kobayashi K; Inoue K; Takada M
  • Organizer: International Workshop Monitoring and manipulating brain function in non-human primates
  • Int'l Joint Research
• [Presentation] Development of viral vectors for delivering functional molecules into nonhuman primate brains (2018)
  • Author(s): Inoue K
  • Organizer: International Workshop Monitoring and manipulating brain function in non-human primates
  • Int'l Joint Research
• [Presentation] マーモセットの帯状皮質を巡る基底核連関構築様式 (2018)
  • Author(s): 上園志織、田辺創思、藤原真紀、柘植仁美、中村克樹、井上謙一、高田昌彦
  • Organizer: 第33回 日本大脳基底核研究会
• [Presentation] Manipulation of primate neural networks by means of modified viral vectors (2018)
  • Author(s): Inoue K
  • Organizer: International Conference Viral Manipuration in Monkey
  • Int'l Joint Research / Invited
• [Presentation] 高精細全脳イメージング技術FASTの開発と精神疾患モデルマウスの病態解析 ―脳全体を対象とした仮説フリーな病態・薬物治療機序の組織学的解析― (2018)
  • Author(s): 勢力薫、笠井淳司、丹生光咲、田沼将人、五十嵐久人、中澤敬信、山口瞬、井上謙一、高田昌彦、橋本均
  • Organizer: 第68回日本薬学会近畿支部総会・大会
• [Presentation] Monkey Features Location Identfication Using Convolutional Neural Networks (2018)
  • Author(s): Labuguen R; Gaurav V; Blanco SN; Matsumoto J; Inoue K; Shibata T
  • Organizer: 第28回日本神経回路学会・全国大会
• [Presentation] Optogenetic activation of cutaneous and proprioceptive afferent in the rat sciatic nerve. (2018)
  • Author(s): Kubota S; Sidikejiang W; Kudoh M; Inoue K; Umeda T; Takada M; Seki K
  • Organizer: Neuroscience 2018
  • Int'l Joint Research
• [Presentation] Pathway-selective manipulation of neural network in primates. (2018)
  • Author(s): Inoue K
  • Organizer: フライブルグ大学セミナー
  • Invited
• [Presentation] Development of viral vectors for delivering functional molecules into nonhuman primate brains. (2018)
  • Author(s): Inoue K
  • Organizer: Genetic technologies for systems neurosciences in non-human primates
  • Int'l Joint Research / Invited
• [Presentation] Pathway-selective manipulation of primate neural networks by means of modified viral vectors. (2018)
  • Author(s): Inoue K
  • Organizer: ピッツバーグ大学大学セミナー
  • Invited
• [Presentation] サル腹側中脳は一次運動野を介して筋出力を促通する. (2018)
  • Author(s): 鈴木迪諒、井上謙一、中川浩、伊佐正、高田昌彦、西村幸男
  • Organizer: 次世代脳プロジェクト冬のシンポジウム
• [Presentation] マーモセットにおける大脳基底核および小脳から前部・後部帯状皮質への多シナプス性入力様式 (2018)
  • Author(s): 上園志織、田辺創思、藤原真紀、柘植仁美、中村克樹、井上謙一、高田昌彦
  • Organizer: 第7回日本マーモセット研究会大会
• [Presentation] 化学遺伝学とPET イメージングの融合による黒質線条体ドーパミン神経活動制御 (2018)
  • Author(s): 南本敬史、三村喬生、永井裕司、井上謙一、須原哲也、高田昌彦
  • Organizer: 第7回日本マーモセット研究会大会
• [Presentation] 狂犬病ウイルスゲノムの分節化 (2017)
  • Author(s): 日高侑也、小林由紀、伊藤睦代、林昌宏、井上謙一、伊藤琢也
  • Organizer: 第16回狂犬病研究会
• [Presentation] Cells of origin of multisynaptic projections from amygdala to ventral premotor cortex in macaques (2017)
  • Author(s): Ishida H; Inoue K; Hoshi E; Takada M
  • Organizer: Different elements of Primate Neural Networks in the Connectome Era
  • Int'l Joint Research
• [Presentation] Organization of multisynaptic inputs from the basal ganglia to the premotor cortex in macaque monkeys - Retrograde transneuronal dual tracing using rabies viral vectors. (2017)
  • Author(s): Inoue K; Fujiwara M; Uezono S; Tanabe S; Ishida H; Hoshi E; Takada M
  • Organizer: Cold Spring Harbor Asia Conference: Primate Neuroscience
  • Int'l Joint Research
• [Presentation] 霊長類および齧歯類の線条体入力系におけるFuGシュードタイプレンチウイルスベクターの遺伝子導入特性 (2017)
  • Author(s): 田辺 創思、井上 謙一、上園 志織、柘植 仁美、中村 克樹、小林 和人、高田 昌彦
  • Organizer: 第32回 日本大脳基底核研究会
• [Presentation] マーモセットにおける大脳基底核から帯状皮質への多シナプス性入力様式 (2017)
  • Author(s): 上園 志織、田辺 創思、藤原 真紀、柘植 仁美、中村 克樹、井上 謙一、高田 昌彦
  • Organizer: 第32回 日本大脳基底核研究会
• [Presentation] Differences in retrograde gene transfer efficiency and cytotoxicity between lentiviral vectors pseudotyped with FuG-E and FuG-B2 glycoprotein in the striatal input system of rodents and primates. (2017)
  • Author(s): Tanabe S; Uezono S; Tsuge H; Fujiwara M; Nagaya K; Nagaya N; Sugawara M; Miwa M; Konoike N; Kato S; Nakamura K; Kobayasi K; Inoue K; Takada M
  • Organizer: 第40回日本神経科学大会
• [Presentation] Phase-amplitude coupling in cerebro-basal ganglia-cerebellar networks: A new model of hypo- and hyperkinetic movement disorders. (2017)
  • Author(s): Ninomiya T; Nagai Y; Inoue K; Hori Y; Kikuchi E; Lee J; Suhara T; Iriki A; Minamimoto T; Takada M; Isoda M; Matsumoto M; Mccairn K.W.
  • Organizer: 第40回日本神経科学大会
• [Presentation] Organization of multisynaptic inputs from the basal ganglia to the dorsal and ventral premotor cortices in macaque monkeys: retrograde transneuronal dual tracing with fluorescent rabies viral vectors. (2017)
  • Author(s): Inoue K; Fujiwara M; Uezono S; Tanabe S; Tsuge H; Nagaya N; Nagaya K; Ishida H; Hoshi E; Takada M
  • Organizer: 第40回日本神経科学大会
• [Presentation] The lentiviral vector pseudotyped with FuG-E glycoprotein is more suitable; compared with the FuG-B2 type; for retrograde gene transfer into the cortical input system of primates. (2017)
  • Author(s): Tsuge H; Uezono S; Tanabe S; Fujiwara M; Nagaya K; Nagaya N; Sugawara M; Miwa M; Konoike N; Kato S; Nakamura K; Kobayashi K; Inoue K; Takada M
  • Organizer: 第40回日本神経科学大会
• [Presentation] Organization of multisynaptic inputs from the basal ganglia and cerebellum to the cingulate cortex in common marmosets as revealed by retrograde transneuronal labeling with rabies virus. (2017)
  • Author(s): Uezono S; Tsuge H; Tanabe S; Fujiwara M; Nagaya N; Nagaya K; Inoue K; Takada M
  • Organizer: 第40回日本神経科学大会
• [Presentation] Pathway-selective manipulation of neural circuits (2017)
  • Author(s): Inoue K
  • Organizer: NIMH Workshop Neural Circuits: Gaps and Opportunities
  • Int'l Joint Research / Invited
• [Presentation] 狂犬病ウイルスゲノムの分節化 (2017)
  • Author(s): 日高 侑也、林 昌宏、伊藤 睦代、朴 天鍋、君付 和範、井上 謙一、小林 由紀、伊藤 琢也
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] Analysis of epitope on rabies virus phosphoprotein recognized by a single chain variable fragment which inhibits rabies virus growth. (2017)
  • Author(s): Kaku Y; Inoue K; Inoue S; Noguchi A; Mirikawa S
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] Pathway-selective optogenetics for elucidating neural network function in primates. (2017)
  • Author(s): Inoue K
  • Organizer: Neuroscience 2017
  • Int'l Joint Research / Invited
• [Presentation] Arrangement of multisynaptic inputs from the basal ganglia to the dorsal and ventral premotor cortical areas in macaques: retrograde transneuronal double labeling with fluorescent rabies viral vectors. (2017)
  • Author(s): Inoue K; Fujiwara M; Uezono S; Tanabe S; Ishida H; Hoshi E; Takada M
  • Organizer: Neuroscience 2017
  • Int'l Joint Research
• [Presentation] FAST; High-speed serial-sectioning imaging for whole brain analysis with high scalability. (2017)
  • Author(s): Kasai A; Seiriki K; Hashimoto T; Niu M; Yamaguchi S; Naka Y; Igarashi H; Tamura M; Nakazawa T; Inoue K; Takada M; Fujita K; Hashimoto H
  • Organizer: Neuroscience 2017
  • Int'l Joint Research
• [Presentation] Organization of multisynaptic inputs from the basal ganglia and cerebellum to the anterior and posterior cingulate cortical areas in common marmosets: Retrograde transneuronal double labeling with fluorescent rabies viral vectors. (2017)
  • Author(s): Uezono S; Tanabe S; Fujiwara M; Tsuge H; Nakamura K; Inoue K; Takada M
  • Organizer: Neuroscience 2017
  • Int'l Joint Research
• [Presentation] Optogenetic modulation of saccade-controlling circuits in the monkey basal ganglia (2017)
  • Author(s): Amita H; Kim H.F; Inoue K; Takada M; Hikosaka O
  • Organizer: Neuroscience 2017
  • Int'l Joint Research
• [Presentation] Differences in retrograde gene transfer efficiency and cytotoxicity between lentiviral vectors pseudotyped with FuG-E and FuG-B2 glycoprotein in rodent and primate brains. (2017)
  • Author(s): Tanabe S; Tsuge H; Uezono S; Fujiwara M; Miwa M; Konoike N; Kato S; Nakamura K; Kobayashi K; Inoue K; Takada M
  • Organizer: Neuroscience 2017
  • Int'l Joint Research
• [Presentation] Cells of origin of multisynaptic projections from amygdala to ventral premotor cortex in macaques (2017)
  • Author(s): Ishida H; Inoue K; Hoshi E; Takada M
  • Organizer: 平成29年度生理研研究会 第7回社会神経科学研究会
• [Presentation] 霊長類における光遺伝学を利用した神経回路操作 (2017)
  • Author(s): 井上 謙一
  • Organizer: 日本学術振興会 光電相互変換第125委員会 第239回研究会 「光技術が切り開く新しいバイオサイエンス・テクノロジー」
  • Invited
• [Remarks] 統合脳システム分野ホームページ
  • URL: http://www.pri.kyoto-u.ac.jp/sections/systems_neuroscience/index.html