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栄養バランスの変化に応じて発生タイミングを調整する適応能力の解明

Publicly Offered Research

Project AreaInterplay of developmental clock and extracellular environment in brain formation
Project/Area Number 17H05766
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKyoto University

Principal Investigator

上村 匡  京都大学, 生命科学研究科, 教授 (80213396)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywords栄養バランス / 発生タイミング / 成長 / ショウジョウバエ / 遺伝子発現制御 / 代謝恒常性 / RNA-seq / メタボローム
Outline of Annual Research Achievements

申請者らはショウジョウバエ近縁種の中で、多種類の果物をエサとする広食性種(D. melを含む)に比べて、単一の植物のみをエサとする狭食性種の方が、高炭水化物食に対する適応能力が低い(発生率が低下し発生タイミングが顕著に遅れる)ことを発見した。この適応能力の違いを生み出す全身性上流シグナル経路の候補として、広食性種 D. melanogaster で近年報告された炭水化物への応答機構、TGF-beta/Activin シグナル伝達経路に着目して研究し、以下の結果を得た。
広食性種 D. melanogaster の野生型は、炭水化物への応答機構を介して全身の各組織で様々な代謝酵素遺伝子の発現量を調節し、異なる餌条件下でも代謝の恒常性を維持できる。一方、狭食性種 D. sechellia と広食性種 D. melanogaster の Activin変異体ではこのような制御が働かず、高炭水化物条件下で代謝酵素遺伝子群の発現が上昇し、多数の代謝産物の量が増加することがわかった。Activin シグナル伝達経路に依存した遺伝子発現制御は、筋肉、脂肪体、腸のいずれにおいても検出された。狭食性種は、高炭水化物食条件下では血リンパ液が酸性化して適応できなくなる可能性が考えられた。エサに酸を添加する実験の結果から、この可能性を支持する結果が得られた。
広食性種と狭食性種のいずれの種にも、TGF-beta/Activin シグナル伝達経路を構成する遺伝子は保存されているにもかかわらず、両者の間で高炭水化物食への適応に大きな差がなぜ生じるのかを、広食性種と狭食性種との雑種を作製してけ調べた。その結果、狭食性種が高炭水化物食に適応できない原因は、このシグナル伝達経路を構成する遺伝子のいずれか一遺伝子の機能欠損には帰着しないことが示唆された。

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (38 results)

All 2020 2019 2018 2017 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (33 results) (of which Int'l Joint Research: 11 results,  Invited: 6 results) Remarks (2 results)

  • [Journal Article] 種間の栄養環境への適応能力の差を生む炭水化物応答機構2020

    • Author(s)
      渡辺佳織、上村匡、服部佑佳子
    • Journal Title

      実験医学

      Volume: 2月号 Pages: 471-474

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Interspecies Comparative Analyses Reveal Distinct Carbohydrate-Responsive Systems among Drosophila Species.2019

    • Author(s)
      Kaori Watanabe, Yasutetsu Kanaoka, Shoko Mizutani, Hironobu Uchiyama, Shunsuke Yajima, Masayoshi Watada, Tadashi Uemura, Yukako Hattori
    • Journal Title

      Cell Reports

      Volume: 28(10) Issue: 10 Pages: 2594-2607

    • DOI

      10.1016/j.celrep.2019.08.030

    • NAID

      120006716587

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DeTerm: Software for automatic detection of neuronal dendritic branch terminals via an artificial neural network.2019

    • Author(s)
      Yasutetsu Kanaoka†, Henrik Skibbe†, Yusaku Hayashi, *Tadashi Uemura and *Yukako Hattori
    • Journal Title

      Genes Cells

      Volume: Jul;24(7) Issue: 7 Pages: 464-472

    • DOI

      10.1111/gtc.12700

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] The nutritional basis of Drosophila associated yeasts and bacteria for larval growth.2020

    • Author(s)
      Ayumi Mure
    • Organizer
      第4回京都生体質量分析研究会シンポジウム(KBMSS)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Nutrient-dependent responses of somatosensory neurons in Drosophila larvae: their underlying mechanisms and impacts on physiological functions.2020

    • Author(s)
      Yasutetsu Kanaoka
    • Organizer
      第4回京都生体質量分析研究会シンポジウム(KBMSS)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Interspecies comparative analyses reveal distinct carbohydrate-responsive systems among Drosophila species.2020

    • Author(s)
      Kaori Watanabe
    • Organizer
      第4回京都生体質量分析研究会シンポジウム(KBMSS)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 成長期の栄養履歴の違いがどのようにして寿命の差を生むか2020

    • Author(s)
      水谷祥子
    • Organizer
      Kyoto Biomolecular Mass Spectrometry Society(KBMSS)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Interspecies comparative analyses reveal distinct carbohydrate-responsive systems among Drosophila species.2020

    • Author(s)
      Kaori Watanabe
    • Organizer
      EMBO | EMBL Symposium "The Organism and its Environment"
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of nutrient-dependent responses of somatosensory neurons in Drosophila larvae: their underlying mechanisms and adaptive roles.2019

    • Author(s)
      金岡泰哲
    • Organizer
      the 52nd annual meeting of the Japanese Society of Developmental Biologists (JSDB)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 個体成長を支える共生微生物の栄養基盤解明に向けて。2019

    • Author(s)
      服部佑佳子
    • Organizer
      京都大学第21回生命科学研究科シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] The nutritional basis of Drosophila associated microbes for larval growth.2019

    • Author(s)
      牟禮あゆみ
    • Organizer
      26th European Drosophila Research Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nutrient-dependent responses of somatosensory neurons in Drosophila larvae: their underlying mechanisms and impacts on physiological functions.2019

    • Author(s)
      金岡泰哲
    • Organizer
      26th European Drosophila Research Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Interspecies comparative analyses reveal distinct carbohydrate-responsive systems among Drosophila species.2019

    • Author(s)
      渡辺佳織
    • Organizer
      26th European Drosophila Research Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 個体成長を支えるショウジョウバエ共生酵母・共生細菌の栄養基盤の解析。2019

    • Author(s)
      牟禮あゆみ
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 感覚神経細胞における栄養応答の分子機構と個体生理機能に果たす役割の追究2019

    • Author(s)
      金岡泰哲
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 成長期の栄養履歴の違いがどのようにして寿命の差を生むか2019

    • Author(s)
      水谷祥子
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Nutri-developmental biology: nutritional adaptability and adipose tissue remodeling.2019

    • Author(s)
      Tadashi Uemura
    • Organizer
      The 9th Federation of the Asian and Oceanian Physiological Society Congress
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] A robust metabolic regulatory system governs nutritional adaptability of Drosophila species.2019

    • Author(s)
      渡辺 佳織
    • Organizer
      第 3 回京都生体質量分析研究会シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 個体の成長と成熟を司る栄養応答システムの解明新学術創成研究機構2018

    • Author(s)
      上村 匡
    • Organizer
      革新的統合バイオ研究コア 栄養・代謝研究ワークショップ「生体トランジスタシスを司る栄養応答システムの動作基盤」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 栄養発生生物学 (Nutri-developmental biology) ―動物の発生を調節する栄養環境への応答機構―2018

    • Author(s)
      上村 匡
    • Organizer
      群馬大学内分泌・代謝学共同利用・共同研究拠点セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 栄養環境が成長期と後期ライフステージに与える影響 シンポジウム「生物は加齢とともにどのように変化するのか: 最先端研究から見えてきたもの」2018

    • Author(s)
      上村 匡
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Differential TGFβ/Activin signaling activity underlies distinct adaptive responses to nutrient balances between generalist and specialist Drosophila species.2018

    • Author(s)
      Kaori Watanabe
    • Organizer
      the 16th International Student Seminar
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Deciphering regulatory mechanisms of distinct responses to nutrient balances between generalist and specialist species.2018

    • Author(s)
      Kaori Watanabe
    • Organizer
      CDB Symposium 2018 “Dynamic Homeostasis: from Development to Aging”
    • Related Report
      2017 Annual Research Report
  • [Presentation] Systemic TGFβ/Activin signaling governs adaptive metabolic responses to nutrient balances of Drosophila species.2018

    • Author(s)
      Yukako Hattori
    • Organizer
      CDB Symposium 2018 “Dynamic Homeostasis: from Development to Aging”
    • Related Report
      2017 Annual Research Report
  • [Presentation] Systemic TGFβ/Activin signaling underlies distinct dietary responses between generalist and specialist species.2018

    • Author(s)
      Kaori Watanabe
    • Organizer
      The 17th International Joint Mini-Symposium on Molecular and Cell Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Interspecies comparative approach uncovers an adaptive system to various nutritional environments.2018

    • Author(s)
      Kaori Watanabe
    • Organizer
      13th Drosophila Research Conference.
    • Related Report
      2017 Annual Research Report
  • [Presentation] 頑健な代謝制御機構が支えるショウジョウバエ近縁種群の栄養環境への適応。2018

    • Author(s)
      渡辺 佳織
    • Organizer
      定量生物学の会第9回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The molecular basis of distinct adaptive responses to nutrient balances between generalist and specialist species.2017

    • Author(s)
      Tadashi Uemura
    • Organizer
      European Molecular Biology Laboratory Symposia "Metabolism in Time and Space: Emerging Links to Cellular and Developmental Programs"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The molecular basis of distinct adaptive responses to nutrient balances between generalist and specialist species.2017

    • Author(s)
      Tadashi Uemura
    • Organizer
      The 3rd International Insect Hormone Workshop
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 個体成長において栄養バランス変化への適応を支える全身性シグナル経路 Systemic signaling for adaptation to nutrient balances in animal growth2017

    • Author(s)
      上村 匡
    • Organizer
      第40回日本分子生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Deciphering distinct responses to nutrient balances between generalist and specialist species by comparative multi-omics approaches.2017

    • Author(s)
      Kaori Watanabe
    • Organizer
      the 4th Asia-Pacific Drosophila Research Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The molecular basis of distinct responses to nutrient balances between generalist and specialist species. 栄養バランス変化に適応し成長する分子機構の解明に向けて2017

    • Author(s)
      服部佑佳子
    • Organizer
      日本発生生物学会第50回大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Deciphering regulatory mechanisms of distinct responses to nutrient balances between generalist and specialist species.2017

    • Author(s)
      Kaori Watanabe
    • Organizer
      the 25th European Drosophila Research Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Systemic signaling for distinct adaptive responses to nutrient balances between generalist and specialist species.2017

    • Author(s)
      Yukako Hattori
    • Organizer
      the 25th European Drosophila Research Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Systemic signaling for distinct adaptive responses to nutrient balances between generalist and specialist species.2017

    • Author(s)
      Yukako Hattori
    • Organizer
      MRC LMS/Kyoto University SPIRITS Program Workshop
    • Related Report
      2017 Annual Research Report
  • [Presentation] Deciphering regulatory mechanisms of distinct responses to nutrient balances between generalist and specialist species.2017

    • Author(s)
      Kaori Watanabe
    • Organizer
      MRC LMS/Kyoto University SPIRITS Program Workshop
    • Related Report
      2017 Annual Research Report
  • [Remarks] 京都大学大学院生命科学研究科 多細胞体構築学講座 細胞認識学分野 上村研究室

    • URL

      http://www.cellpattern.lif.kyoto-u.ac.jp/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      http://www.cellpattern.lif.kyoto-u.ac.jp/

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2021-01-27  

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