花幹細胞の時空間性を司る複合鍵の解析

• Project Area: Determining the principles of the birth of new plant species: molecular elucidation of the lock-and-key systems in sexual reproduction
• Project/Area Number: 17H05843
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: 伊藤 寿朗 奈良先端科学技術大学院大学, 先端科学技術研究科, 教授 (90517096)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000); Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: 発現制御 / 幹細胞 / エピジェネティクス / 発生・分化 / 構造

Outline of Annual Research Achievements

本研究の目的は、生殖器官の形成過程における転写因子とエピジェネティック因子複合体の作用機構の解析を通して、時空間特異性の制御機構を理解することである。これまでに花発生過程において、幹細胞の増殖停止に機能する転写因子の複合体に着目し、それらのターゲット遺伝子の選択性を解析してきた。転写因子であるSUPERMANは花発生初期において、花幹細胞群を取り囲むように発現している。SUPタンパク質は、ポリコム因子であるFIEと直接結合して、オーキシン合成酵素YUUCCA (YUC)遺伝子の発現抑制をおこなっている事、実際にオーキシンの生合成を介して花幹細胞の増殖を抑制していることを示した (EMBO Journal, 2018)。また、CRC転写因子はSUPから2日ほど遅れて、幹細胞を取り囲むように発現する。CRCもまたYUCを直接制御しており、CRCの上流因子であるAGとともにYUC遺伝子座に直接結合する。すなわち、AGはまずクロマチンリモデリング因子の導入により、クロマチン構造を開き、その後にCRCが直接結合することで、YUCを活性化している。そのクロマチンを介したオーキシン合成酵素の制御機構の論文をNature Communications (2018)にて発表した。さらに、細胞自立的に花幹細胞の増殖抑制にかかわる経路の解析を行い、KNUがクロマチンリモデリング因子の結合阻害と抑制的ヒストン修飾酵素の導入という多段階のエピジェネティック制御を介した遺伝子ネットワーク機構を解明して論文報告を行った（Plant Cell 2019 in press）。以上、花幹細胞の増殖抑制の時空間性の制御として、転写因子とヒストン修飾酵素との精緻なクロストークによるエピジェネティク制御機構を解明することができた。

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Abscisic acid‐dependent histone demethylation during postgermination growth arrest in Arabidopsis (2019)
  • Author(s): Wu Jinfeng、Ichihashi Yasunori、Suzuki Takamasa、Shibata Arisa、Shirasu Ken、Yamaguchi Nobutoshi、Ito Toshiro
  • Journal Title: Plant, Cell & Environment Volume: - Issue: 7 Pages: 2198-2214
  • DOI: 10.1111/pce.13547
  • Peer Reviewed / Open Access
• [Journal Article] Integration of transcriptional repression and Polycomb-mediated silencing of WUSCHEL in floral meristems (2019)
  • Author(s): Sun Bo、Zhou Yingying、Cai Jie、Shang Erlei、Yamaguchi Nobutoshi、Xiao Jun、Looi Liang-Sheng、Wee Wan-Yi、Gao Xiuying、Wagner Doris、Ito Toshiro
  • Journal Title: The Plant Cell Volume: - Issue: 7 Pages: 1488-1505
  • DOI: 10.1105/tpc.18.00450
  • NAID: 120006726651
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] When to stop: an update on molecular mechanisms of floral meristem termination (2019)
  • Author(s): Yifeng Xu, Nobutoshi Yamaguchi, Eng-Seng Gan, Toshiro Ito
  • Journal Title: Journal of Experimental Botany Volume: 70 Issue: 6 Pages: 1711-1718
  • DOI: 10.1093/jxb/erz048
  • Peer Reviewed
• [Journal Article] Histone demethylases control root elongation in response to stress-signaling hormone abscisic acid (2019)
  • Author(s): Wu Jinfeng、Yamaguchi Nobutoshi、Ito Toshiro
  • Journal Title: Plant Signaling & Behavior Volume: - Issue: 7 Pages: 1-4
  • DOI: 10.1080/15592324.2019.1604019
  • Peer Reviewed
• [Journal Article] Chromatin-mediated feed-forward auxin biosynthesis in floral meristem determinacy. (2018)
  • Author(s): Yamaguchi, N., Huang, J., Tatsumi, Y., Abe, M., Sugano, S. S., Kojima, M., Takebayashi, Y., Kiba, T., Yokoyama, R., Nishitani, K., Sakakibara, H., and Ito, T.
  • Journal Title: Nature Commun. Volume: 9 Issue: 1 Pages: 5290-5290
  • DOI: 10.1038/s41467-018-07763-0
  • NAID: 120006549567
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Meristem Termination and Organ Number Control in Early Stage of Arabidopsis Flower Development (2018)
  • Author(s): Xu Yifeng、Ito Toshiro
  • Journal Title: Journal of Cell Signaling Volume: 03 Issue: 03
  • DOI: 10.4172/2576-1471.1000186
  • Peer Reviewed
• [Journal Article] In Situ Proximity Ligation Assay to Detect the Interaction Between Plant Transcription Factors and Other Regulatory Proteins (2018)
  • Author(s): Yifeng Xu, Eng-Seng Gan, Toshiro Ito
  • Journal Title: Plant Transcription Factors.Methods in molecular biology (Clifton, N.J.) Volume: 1830 Pages: 325-335
  • DOI: 10.1007/978-1-4939-8657-6_19
  • ISBN: 9781493986569, 9781493986576
  • Peer Reviewed
• [Journal Article] Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis (2018)
  • Author(s): Ze Hong Lee, Nobutoshi Yamaguchi, Toshiro Ito
  • Journal Title: Plant Transcription Factors.Methods in molecular biology (Clifton, N.J.) Volume: 1830 Pages: 93-108
  • DOI: 10.1007/978-1-4939-8657-6_6
  • ISBN: 9781493986569, 9781493986576
  • Peer Reviewed
• [Journal Article] RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells (2018)
  • Author(s): Arai Satoshi、Kriszt Rokus、Harada Kazuki、Looi Liang-Sheng、Matsuda Shogo、Wongso Devina、Suo Satoshi、Ishiura Shoichi、Tseng Yu-Hua、Raghunath Michael、Ito Toshiro、Tsuboi Takashi、Kitaguchi Tetsuya
  • Journal Title: Angewandte Chemie Volume: 130 Issue: 34 Pages: 11039-11044
  • DOI: 10.1002/ange.201804304
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] SUPERMAN regulates floral whorl boundaries through control of auxin biosynthesis. (2018)
  • Author(s): Xu, Y., Prunet, N., Gan, E-S., Wang, Y., Stewart, D., Wellmer, F., Huang, J., Yamaguchi, N., Tatsumi, Y., Kojima, M., Kiba, T., Sakakibara, H., Jack, T.P., Meyerowitz, E.M., Ito, T.
  • Journal Title: EMBO Journal Volume: - Issue: 11
  • DOI: 10.15252/embj.201797499
  • NAID: 120006478564
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Membrane-bound NAC-like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa (2018)
  • Author(s): Guo, S., Dai, S., Singh, P.K., Wang, H., Wang, Y., Tan, L.H., Wee, W., and Ito, T.
  • Journal Title: Frontiers in Plant Science, Plant Biotechnology Volume: 9 Pages: 555-555
  • DOI: 10.3389/fpls.2018.00555
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of floral meristem activity through the interaction of AGAMOUS, SUPERMAN, and CLAVATA3 in Arabidopsis. (2018)
  • Author(s): Uemura, A., Yamaguchi, N, Xu, Y., Wee, W-Y., Ichihashi, Y., Suzuki, T., Shibata, A., Shirasu, K., and Ito, T.
  • Journal Title: Plant Reproduction Volume: 31 Issue: 1 Pages: 89-105
  • DOI: 10.1007/s00497-017-0315-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Membrane-bound NAC-like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa (2018)
  • Author(s): Guo, S., Dai, S., Singh, P.K., Wang, H., Wang, Y., Tan, L.H., Wee, W., and Ito, T.,
  • Journal Title: Frontiers in Plant Science, Plant Biotechnology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] In situ proximity ligation assay to detect the interaction between plant transcription factors and other regulatory proteins (2018)
  • Author(s): Xu, Y., Gan, E.S., and Ito, T.
  • Journal Title: Methods in Molecular Biology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Fine-tuning of auxin homeostasis governs the transition from floral stem cell maintenance to gynoecium formation. (2017)
  • Author(s): Yamaguchi, N., Huang, J., Xu, Y., Tanoi, K., and Ito, T.
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 1125-1125
  • DOI: 10.1038/s41467-017-01252-6
  • NAID: 120006364326
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Gene network controlling flower development in Arabidopsis. (2017)
  • Author(s): Kadoya, Y., Yamaguchi, N., Ito, T.
  • Journal Title: 化学と生物 Volume: 55 Pages: 602-610
  • Peer Reviewed
• [Presentation] Multistep termination of floral stem cell activities (2019)
  • Author(s): 伊藤 寿朗
  • Organizer: Japan-Taiwan Plant Biology 2019 (JTPB2019)
  • Int'l Joint Research / Invited
• [Presentation] Spatio-temporal regulation of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: PSDB 10th Annual National Convention
  • Int'l Joint Research / Invited
• [Presentation] Memory and induced forgetting in plants (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: University of the Philippines-Diliman Ateneo de University 大学訪問セミナー
• [Presentation] 植物における記憶定着と能動的忘却システム (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: さきがけ懇親会
• [Presentation] Spatio-temporal regulation of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: Henan University大学訪問セミナー
  • Invited
• [Presentation] Multi-step termination of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: 福建農林大学さくらサイエンスプラン
• [Presentation] Auxin-mediated multistep termination of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: IPMB 2018
  • Int'l Joint Research / Invited
• [Presentation] 花幹細胞の時空間的な運命制御 (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: 京都大学形態統御学分科セミナー
  • Invited
• [Presentation] Auxin-mediated floral meristem determinacy control (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: 25th ICSPR 2018
  • Int'l Joint Research / Invited
• [Presentation] Auxin-mediated multistep termination of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: Meristem 2018
  • Int'l Joint Research / Invited
• [Presentation] 花幹細胞の多段階での終結機構 (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: 日本植物生理学会
  • Invited
• [Presentation] 花幹細胞の時空間的な運命制御 (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: 東京大学 生物科学セミナー
  • Invited
• [Presentation] Floral stem cell termination and differentiation (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: Temasek Life Sciences Laboratory
  • Invited
• [Presentation] Multi-step termination of floral stem cell activities in Arabidopsiｓ (2017)
  • Author(s): 伊藤 寿朗
  • Organizer: University of Georgia & University of California Davis
  • Invited
• [Presentation] Multi-step termination of floral stem cell activities in Arabidopsis (2017)
  • Author(s): 伊藤 寿朗
  • Organizer: Cold Spring Harbor meeting “Plant Genome & biotechnology”
  • Int'l Joint Research / Invited
• [Presentation] Multi-step , termination of floral stem cell activities (2017)
  • Author(s): 伊藤 寿朗
  • Organizer: Philippine Ateneo De Manila University and University of Philippine, Diliman
  • Invited
• [Presentation] Multi-step termination of floral stem cell activities (2017)
  • Author(s): 伊藤 寿朗
  • Organizer: Japan-France Meeting GDRI Integrative Plant Biology “The developing plant in its environment”
  • Int'l Joint Research / Invited
• [Presentation] Coordination of plant growth and differentiation at fluctuating temperatures (2017)
  • Author(s): 伊藤 寿朗
  • Organizer: The 3rd TLL-NAIST-Joint symposium
  • Invited
• [Presentation] Auxin-mediated dual-step termination of floral stem cells (2017)
  • Author(s): 伊藤 寿朗
  • Organizer: CSH-Asia Conference: Plant Cell & Developmental Biology
  • Invited
• [Presentation] Conference “Epigenetic timer to coordinate growth and differentiation in plants (2017)
  • Author(s): 伊藤 寿朗
  • Organizer: The 50th Japan Developmental Biology Conference
  • Invited
• [Remarks] 伊藤研究室ホームページ
  • URL: http://bsw3.naist.jp/ito/