個性的ジェンダーのニューロン機構

• Project Area: Integrative research toward elucidation of generative brain systems for individuality
• Project/Area Number: 17H05935
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: National Institute of Information and Communications Technology (2018); Tohoku University (2017)
• Principal Investigator: 山元 大輔 国立研究開発法人情報通信研究機構, 未来ICT研究所, 上席研究員 (50318812)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 神経可塑性 / 経験依存的神経活動 / 行動学

Outline of Annual Research Achievements

P1ニューロン群は、我々が世界で初めてキイロショウジョウバエにおいて同定した雄の求愛開始意志決定ニューロン集団である。P1ニューロンは、片半球あたり20個の雄特異的介在ニューロンで形成されている（Kimura et al., 2008, Neuron 59, 759-769）。実際、本来は雄特異的はP1ニューロンを雌の脳内に人為的に発生させれば、その雌が雄の求愛行動をするようになる。また、野生型成虫羽化後の集団生活経験による雄の求愛行動の抑制も、P1ニューロンの機能的可塑性に依存して生ずる。現在、複数の研究グループの努力によりP1ニューロンに求愛制御のほぼすべての情報が収斂して統合され、P1が主要な求愛決定中枢として働くことが確立しつつあるが、このようにP1がこの特権的機能を発揮できる理由は不明である。野生型の雄を成虫羽化後、数日間隔離して育てると、複数の雄の集団の中で育てた場合に比べて雌への求愛が著しく増強される。この背景にP1ニューロンの行動惹起閾値の低下（受けた刺激が弱くても行動出力をトリガする）があることは、optogeneticsとCa2+ imagingによって示唆されていた（Inagaki et al., 2014, Nat. Methods 11, 325-332）。我々はP1にin vivo patch clampを施し、膜電位固定下で記録される膜電流のうち遅延整流性外向きK+電流（ID）のみが、特異的に集団生活によって、一部のP1ニューロンで劇的に増加することを明らかにした。このことから、羽化直後からの数日を臨界期（臨界点）として社会経験を膜電流に刻印するP1ニューロンが存在し、その働きで求愛行動の経験依存的変容が生ずると推察された。

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] The desaturase1 gene affects reproduction before, during and after copulation in Drosophila melanogaster. (2019)
  • Author(s): Nojima T, Chauvel I, Houot B, Bousquet F, Farine JP, Everaerts C, Yamamoto D, Ferveur JF.
  • Journal Title: J. Neurogenet. Volume: - Issue: 2 Pages: 96-115
  • DOI: 10.1080/01677063.2018.1559843
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Partial proteasomal degradation of Lola triggers the male-to-female switch of a dimorphic courtship circuit. (2019)
  • Author(s): Kosei Sato, Hiroki Ito, Atsushi Yokoyama, Gakuta Toba and Daisuke Yamamoto
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 166-166
  • DOI: 10.1038/s41467-018-08146-1
  • Peer Reviewed / Open Access
• [Journal Article] A novel sex difference in Drosophila contact chemosensory neurons unveiled using single cell labeling (2018)
  • Author(s): Kimura Ken-ichi、Urushizaki Akira、Sato Chiaki、Yamamoto Daisuke
  • Journal Title: Journal of Neurogenetics Volume: - Issue: 2 Pages: 1-9
  • DOI: 10.1080/01677063.2018.1531858
  • Peer Reviewed
• [Journal Article] Antennae sense heat stress to inhibit mating and promote escaping in Drosophila females. (2018)
  • Author(s): Yusuke Miwa, Masayuki Koganezawa & Daisuke Yamamoto
  • Journal Title: J Neurogenet. Volume: 32 Issue: 4 Pages: 353-363
  • DOI: 10.1080/01677063.2018.1513507
  • Peer Reviewed
• [Journal Article] Genetic dissection of stress-induced reproductive arrest in Drosophila melanogaster females (2018)
  • Author(s): Ojima Noriyuki、Hara Yusuke、Ito Hiroki、Yamamoto Daisuke
  • Journal Title: PLOS Genetics Volume: 14 Issue: 6 Pages: e1007434-e1007434
  • DOI: 10.1371/journal.pgen.1007434
  • Peer Reviewed / Open Access
• [Journal Article] The Wolbachia protein TomO interacts with a host RNA to induce polarization defects in Drosophila oocytes. (2018)
  • Author(s): Ote M, Yamamoto D.
  • Journal Title: Arch Insect Biochem Physiol. Volume: 99 Issue: 1
  • DOI: 10.1002/arch.21475
  • Peer Reviewed
• [Journal Article] Enhancing Nanos expression via the bacterial TomO protein is a conserved strategy used by the symbiont Wolbachia to fuel germ stem cell maintenance in infected Drosophila females. (2018)
  • Author(s): Ote M, Yamamoto D
  • Journal Title: Arch Insect Biochem Physiol. Volume: 98 Issue: 3
  • DOI: 10.1002/arch.21471
  • Peer Reviewed
• [Journal Article] Ecdysone signaling regulates specification of neurons with a male-specific neurite in Drosophila. (2018)
  • Author(s): Binglong Zhang, Kosei Sato and Daisuke Yamamoto
  • Journal Title: Biology Open Volume: 7 Issue: 2 Pages: 1-8
  • DOI: 10.1242/bio.029744
  • Peer Reviewed / Open Access
• [Journal Article] Balanced genetic diversity improves population fitness. (2018)
  • Author(s): Takahashi, Y., Tanaka, R., Yamamoto, D., Suzuki, N. and Kawata, M.
  • Journal Title: Proc. R. Soc. B Volume: 285
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] L-leucine and SPNS1 coordinately ameliorate dysfunction of autophagy in mouse and human Niemann-Pick type C disease. (2017)
  • Author(s): Yanagisawa H, Ishii T, Endo K, Kawakami E, Nagao K, Miyashita T, Akiyama K, Watabe K, Komatsu M, Yamamoto D, Eto Y.
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 15994-15994
  • DOI: 10.1038/s41598-017-15305-9
  • Peer Reviewed / Open Access
• [Journal Article] The core-promoter factor TRF2 mediates a Fruitless action to masculinize neurobehavioral traits in Drosophila. (2017)
  • Author(s): Zahid Sadek Chowdhury, Kosei Sato and Daisuke Yamamoto
  • Journal Title: Nature Communications Volume: 1480 Issue: 1 Pages: 1-10
  • DOI: 10.1038/s41467-017-01623-z
  • Peer Reviewed / Open Access
• [Journal Article] Optogenetic Activation of the fruitless-Labeled Circuitry in Drosophila subobscura Males Induces Mating Motor Acts. (2017)
  • Author(s): Ryoya Tanaka, Tomohiro Higuchi, Soh Kohatsu, Kosei Sato and Daisuke Yamamoto
  • Journal Title: Journal of Neuroscience Volume: 37 Issue: 48 Pages: 11662-11674
  • DOI: 10.1523/jneurosci.1943-17.2017
  • Peer Reviewed
• [Journal Article] Ovarian polarity and cell shape determination by Btk29A in Drosophila. (2017)
  • Author(s): Hamada-Kawaguchi, N. and Yamamoto, D.
  • Journal Title: Genesis Volume: 10 Issue: 8
  • DOI: 10.1002/dvg.23042
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Quantitative analysis of visually induced courtship elements in Drosophila subobscura. (2017)
  • Author(s): Higuchi, T., Kohatsu, S., Yamamoto, D.
  • Journal Title: J. Neurogenet. Volume: 印刷中 Issue: 1-2 Pages: 1-9
  • DOI: 10.1080/01677063.2017.1290613
  • Peer Reviewed
• [Presentation] ショウジョウバエの性行動進化解明に向けたEvo-Devo-Neuroアプローチの提唱 (2019)
  • Author(s): 山元大輔
  • Organizer: 第66回日本生態学会大会、シンポジウム
  • Invited
• [Presentation] The fruitless-labelled courtship circuit: conserved and diversified. (2019)
  • Author(s): Yamamoto, D.
  • Organizer: The 2nd Asia-Pacific Drosophila Neuroscience Conference. Academia Sinica, Taipei, Taiwan(招待講演)(国際学会)
  • Int'l Joint Research / Invited
• [Presentation] Plasticity of innate courtship behavior and its neural substrate in flies. (2018)
  • Author(s): Yamamoto, D.
  • Organizer: 第41回日本分子生物学会年会
  • Invited
• [Presentation] ショウジョウバエ非モデル種を用いた求愛行動の進化をもたらす分子・神経メカニズム (2018)
  • Author(s): 田中良弥、樋口智大、古波津創、佐藤耕世、粟崎健、山元大輔
  • Organizer: 第41回日本分子生物学会年会、シンポジウム
  • Invited
• [Presentation] ショウジョウバエ雄の同性愛行動を抑制するDE細胞 (2018)
  • Author(s): 山田芳、田中陵暉、山元大輔、中越秀樹
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] キイロショウジョウバエ運動神経の活動抑制は雄特異的筋の形成を阻害する (2018)
  • Author(s): 木村賢一、熊野梨美、山元大輔
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] Identification of visual interneurons that mediate courtship following in Drosophila. (2018)
  • Author(s): Kohatsu, S. and Yamamoto, D.
  • Organizer: Cold Spring Harbor Asia Conference: Latest Advances in Development & Function of Neuronal Circuits,
  • Int'l Joint Research
• [Presentation] Searching for neurons regulating sexual receptivity in Drosophila females. (2018)
  • Author(s): Miwa, Y., Koganezawa, M. and Yamamoto, D.
  • Organizer: Towards understanding of individuality
• [Presentation] Studying a mini-brain of insects for the understanding of mechanisms underlying perceptual decision-making. (2018)
  • Author(s): Yamamoto, D.
  • Organizer: MOST-NICT Joint Workshop on AI for ICT, GIS NTU Convention Center, Taipei, Taiwan
  • Int'l Joint Research
• [Presentation] 環境ストレスによって雌の性的受容性が変化する機構 (2018)
  • Author(s): 三輪祐輔、小金澤雅之、山元大輔
  • Organizer: 次世代脳プロジェクト第3回シンポジウム、東京
• [Presentation] A brain interneuron shaping the species-specific courtship pattern in Drosophila. (2018)
  • Author(s): Koganezawa, M., Suzuki, T. and Yamamoto, D.
  • Organizer: 第40回日本比較生理生化学会大会、神戸
• [Presentation] Identification of interneuron subsets that mediate vision-dependent courtship following in Drosophila (2018)
  • Author(s): Kohatsu, S. and Yamamoto, D.
  • Organizer: 第40回日本比較生理生化学会大会、神戸
• [Presentation] Acclimatization to cold temperature of brain insulin neurons drives reproductive dormancy (2018)
  • Author(s): Hara, Y., Ojima, N., Ito, H. and Yamamoto, D.
  • Organizer: 第13回日本ショウジョウバエ研究会、京都
• [Presentation] The neural mechanism underlying evolution of mating preferenceThe 46th Naito Conference: Mechanisms of Evolution and Biodiversity (2018)
  • Author(s): Ishikawa, Y., Maeda, N., Mochizuki, K., Nomura, G., Kamikouchi, A. and Yamamoto, D.
  • Organizer: The 46th Naito Conference: Mechanisms of Evolution and Biodiversity
  • Int'l Joint Research
• [Presentation] An attempt to identify the neurons that are responsible for species-specific behavior using the flip-out mosaicism in Drosophila subobscura. (2018)
  • Author(s): Tanaka, R., Higuchi, T., Kohatsu, S., Sato, K., Awasaki, T. and Yamamoto, D.
  • Organizer: 第13回日本ショウジョウバエ研究会、京都
• [Presentation] 行動の種特異性を規定する神経メカニズム-ショウジョウバエの求愛行動を用いた取り組み (2018)
  • Author(s): 田中良弥、樋口智大、古波津創、佐藤耕世、粟崎健、山元大輔
  • Organizer: 日本進化学会第20回大会、東京
• [Presentation] Experience-Dependent Nonsynaptic Plasticity in Drosophila Central Brain Neurons. (2018)
  • Author(s): Rindner, D., Hara, Y. and Yamamoto, D.
  • Organizer: 第41回日本神経科学大会、神戸
• [Presentation] ショウジョウバエ性行動を制御する神経回路と進化 (2018)
  • Author(s): 山元大輔
  • Organizer: 第26回日本ステロイドホルモン学会学術集会、幕張
  • Invited
• [Presentation] ショウジョウバエで解き明かす性行動の脳内回路と遺伝子制御 (2018)
  • Author(s): 山元大輔
  • Organizer: プロゲストーゲン研究会、台場
  • Invited
• [Presentation] 性行動を生み出す脳と遺伝子 (2017)
  • Author(s): 山元大輔
  • Organizer: 第28回 日本性機能学会
  • Invited
• [Presentation] Nature versus nurture in D. melanogaster male courtship (2017)
  • Author(s): Yamamoto, D.
  • Organizer: The 4th Asia-Pacific Drosophila Research Conference
  • Int'l Joint Research / Invited
• [Remarks] 山元研究室
  • URL: http://www2.nict.go.jp/frontier/evoneuro/index.html