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New perspective of lipoquality research: lipid pheromones and olfactory receptors

Publicly Offered Research

Project AreaQuality of lipids in biological systems
Project/Area Number 18H04683
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

吉原 良浩  国立研究開発法人理化学研究所, 脳神経科学研究センター, チームリーダー (20220717)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywords脂質フェロモン / ゼブラフィッシュ / 嗅覚受容体 / 神経回路 / 嗅覚行動 / 性フェロモン / プロスタグランジン / 警報フェロモン / 視床下部
Outline of Annual Research Achievements

脂質はフェロモンとしても機能する。私たちは、排卵期のメスの魚から水中に分泌されるプロスタグランジンF2α(PGF2α)が性フェロモンとして、オスの魚の嗅覚受容体OR114-1/2に作用して、誘引・求愛行動を誘起することを報告した(Nat. Neurosci. 2016)。本研究では、モデル生物としてのゼブラフィッシュの利点を最大限に活用し、生化学・分子生物学・細胞生物学・遺伝学・解剖学・神経活動イメージング・行動学・神経内分泌学など多様な実験手法を統合的に組み合わせ、フェロモンとしての脂質の新たな機能の解明に向けた研究を、嗅上皮・嗅球・高次中枢レベルで行った。以下に2019年度の研究成果を述べる。
(A) 魚類が豊富に産生するエイコサペンタエン酸(EPA)に由来するプロスタグランジンPGF3αを認識するゼブラフィッシュ嗅覚受容体を同定した。
(B) 傷ついた魚の皮膚から水中にから分泌され、同種他個体に対して警報反応(逃避・フリーズ・水底への異動)を協同して引き起こす2つの物質(警報フェロモン)の精製・構造決定に成功した。そのうちの1つは新規の誘導脂質(硫酸化胆汁アルコール)であり、異なった魚種にはそれぞれ特異的な類似構造物質が存在することがわかった。またこの新規誘導脂質が特異的に結合するゼブラフィッシュ嗅覚受容体を同定した。さらに、警報フェロモン入力から忌避行動出力へと至る嗅覚神経回路メカニズムを嗅上皮レベル、嗅球レベル、さらには高次嗅覚中枢レベルで明らかにした。

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (20 results)

All 2020 2019 2018

All Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 6 results) Presentation (13 results) (of which Int'l Joint Research: 5 results,  Invited: 10 results)

  • [Journal Article] The claustrum coordinates cortical slow-wave activity.2020

    • Author(s)
      Narikiyo Kimiya, Mizuguchi Rumiko Ajima Ayako, Shiozaki Momoko, Hamanaka Hiroki, Johansen Joshua P, Mori Kensaku, Yoshihara Yoshihiro
    • Journal Title

      Nature Neuroscience

      Volume: in press

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification of the dendritic filopodia-rich fraction2019

    • Author(s)
      Furutani Yutaka、Yoshihara Yoshihiro
    • Journal Title

      Journal of Visualized Experiments

      Volume: 147 Issue: 147

    • DOI

      10.3791/59292

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Contribution of Individual Olfactory Receptors to Odor-Induced Attractive or Aversive Behavior in Mice2019

    • Author(s)
      Horio N, Murata K, Yoshikawa K, Yoshihara Y, Touhara K.
    • Journal Title

      Nature Communications

      Volume: 10 Issue: 1 Pages: 209-209

    • DOI

      10.1038/s41467-018-07940-1

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Proteomic analysis of dendritic filopodia-rich fraction isolated by telencephalin and vitronectin interaction2018

    • Author(s)
      Furutani Y, Yoshihara Y
    • Journal Title

      Frontiers in Synaptic Neuroscience

      Volume: 10 Pages: 27-27

    • DOI

      10.3389/fnsyn.2018.00027

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sexual rejection via a vomeronasal receptor-triggered limbic circuit2018

    • Author(s)
      Osakada Takuya、Ishii Kentaro K.、Mori Hiromi、Eguchi Ryo、Ferrero David M.、Yoshihara Yoshihiro、Liberles Stephen D.、Miyamichi Kazunari、Touhara Kazushige
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 4463-4463

    • DOI

      10.1038/s41467-018-07003-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of an Intra- and Inter-specific Tear Protein Signal in Rodents2018

    • Author(s)
      Tsunoda Mai、Miyamichi Kazunari、Eguchi Ryo、Sakuma Yasuo、Yoshihara Yoshihiro、Kikusui Takefumi、Kuwahara Masayoshi、Touhara Kazushige
    • Journal Title

      Current Biology

      Volume: 28 Issue: 8 Pages: 1213-1223

    • DOI

      10.1016/j.cub.2018.02.060

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Terminal nerve GnRH3 neurons mediate slow avoidance of carbon dioxide in larval zebrafish.2018

    • Author(s)
      Koide T, Yabuki Y, Yoshihara Y.
    • Journal Title

      Cell Report (2018) 22(5):1115-1123.

      Volume: 22 Issue: 5 Pages: 1115-1123

    • DOI

      10.1016/j.celrep.2018.01.019

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 嗅覚研究30年:分子から回路へ、行動へ2019

    • Author(s)
      吉原良浩
    • Organizer
      第53回日本味と匂学会大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 嗅覚忌避行動の分子・神経回路基盤2019

    • Author(s)
      吉原良浩
    • Organizer
      第3回感覚器研究イニシアチブ・シンポジウム「感覚と脳-基礎から臨床へ-」
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Neural circuit mechanism underlying olfactory memory and motivated behavior in zebrafish2019

    • Author(s)
      Miyasaka Nobuhiko, Yoshihara Yoshihiro
    • Organizer
      第42回日本神経科学大会/日本神経化学会合同大会(NEURO2019)
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Identification of olfactory alarm substances in zebrafish2019

    • Author(s)
      Yoshihara Yoshihiro
    • Organizer
      48th Naito Conference “Integrated Sensory Sciences: Pain, Itch, Smell and Taste”
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The claustrum coordinates cortical slow-wave activity2019

    • Author(s)
      Yoshihara Yoshihiro
    • Organizer
      Society for Claustrum Research Annual Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Comprehensive map of neuropeptide expressions in the adult zebrafish brain2019

    • Author(s)
      Kajiyama Towako, Yoshihara Yoshihiro
    • Organizer
      第42回日本神経科学大会/日本神経化学会合同大会(NEURO2019)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Dissection of neural circuit mediating olfactory alarm reaction in zebrafish2019

    • Author(s)
      Masuda Miwa, Ihara Sayoko, Mori Naoki, Koide Tetsuya, Nobuhiko Miyasaka, Wakisaka Noriko, Yoshikawa Keiich, Watanabe Hidenori, Touhara Kazushige, Yoshihara Yoshihiroi
    • Organizer
      第42回日本神経科学大会/日本神経化学会合同大会(NEURO2019)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 嗅覚記憶と意欲行動の神経回路メカニズム2019

    • Author(s)
      宮坂信彦、吉原良浩
    • Organizer
      第53回日本味と匂学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 魚の嗅覚機能を利用した行動制御技術の開発2018

    • Author(s)
      吉原良浩
    • Organizer
      JST-CRDS科学技術未来戦略ワークショップ
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 魚類の嗅覚行動の神経メカニズム:新規アデノシン嗅覚受容体「A2c」を中心に2018

    • Author(s)
      吉原良浩
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Identification of olfactory alarm substances in zebrafish2018

    • Author(s)
      Yoshihara Y
    • Organizer
      5th Conference on Imaging Structure & Function of the Zebrafish Brain
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Neural circuit mechanism underlying olfactory alarm reaction in zebrafish2018

    • Author(s)
      Yoshihara Y
    • Organizer
      Kavli Institute Seminar
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Olfactory receptors, circuits and behaviors in zebrafish2018

    • Author(s)
      Yoshihara Y
    • Organizer
      8th Biennial Neuroscience Conference “Neurizons 2018”
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2018-04-23   Modified: 2021-01-27  

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