植物の高温・低温ストレス適応におけるRNA顆粒を介した転写後制御機構の解析

• Project Area: Integrative understanding of biological phenomena with temperature as a key theme
• Project/Area Number: 18H04705
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: 関 原明 国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (80281624)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000); Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: 植物の環境ストレス適応 / 高温ストレス / 低温ストレス / ストレス顆粒 / RNA制御

Outline of Annual Research Achievements

シロイヌナズナを用いて高温・低温などの温度ストレス適応におけるプロセッシングボディ(PB)やストレス顆粒(SG)などのRNA顆粒によるmRNAの分解や一時保存制御機構の解析を行い、以下の研究実績が得られた。PBの構成因子であるXRN4の欠損変異体は高温・低温ストレス耐性を示す。その原因遺伝子を特定するために、遺伝子発現解析・分解速度解析を用いて、温度ストレス応答時におけるXRN4のターゲットを検索した。その結果、XRN4は高温ストレス時にはHeat shock factor A2 (HSFA2)、低温ストレス時にはMyeloblastosis transcription factor (MYB TF)をターゲットとしていることが明らかとなり、分解制御を通してmRNAの発現量を調節し、温度ストレスに適応していることが示唆された。一方、SGの因子であるUBP1bは過剰発現させることで高温耐性を示す。遺伝子発現解析・分解速度解析から、原因遺伝子の候補を複数見出した。これらの遺伝子がUBP1bの直接のターゲットであるかRNA免疫沈降(RIP)により確認した。RIP法は、国際共同研究によりJulia Bailey-Serres教授(カリフォルニア大、Riverside校)らから習得し、新しく確立した。その結果、UBP1bに直接結合するターゲットは、DNAJ heat shock protein（DNAJ HSP）、Stress-associated protein （SAP）であることが明らかとなり、これらのmRNAの一時保存制御を介して翻訳のタイミングを調節し、高温ストレスに適応していることが示唆された。以上より、植物の温度ストレス応答・適応には、RNA顆粒によるmRNAの転写後調節・翻訳制御が重要であることが示された。

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] カリフォルニア大、Riverside校(米国)
• [Int'l Joint Research] カリフォルニア大、Riverside校(米国)
• [Journal Article] The dynamic kaleidoscope of RNAs in plants (2020)
  • Author(s): Bailey-Serres, J., Zhai, J. and Seki, M.
  • Journal Title: Plant Physiol. Volume: 182 Issue: 1 Pages: 1-9
  • DOI: 10.1104/pp.19.01558
  • Open Access / Int'l Joint Research
• [Journal Article] Histone modifications form epigenetic regulatory networks to regulate abiotic stress response (2020)
  • Author(s): Ueda, M. and Seki, M.
  • Journal Title: Plant Physiol. Volume: 182 Issue: 1 Pages: 15-26
  • DOI: 10.1104/pp.19.00988
  • Peer Reviewed / Open Access
• [Journal Article] Transcriptome analysis of the hierarchical response of HDAC proteins that respond in an antagonistic manner to salinity stress. (2019)
  • Author(s): Ueda, M., Matsui, A., Watanabe, S., Kobayashi, M., Saito, K., Tanaka, M., Ishida, J., Kusano, M., Seo, M. and Seki, M.
  • Journal Title: Front. Plant Sci. Volume: 10 Pages: 1323-1323
  • DOI: 10.3389/fpls.2019.01323
  • Peer Reviewed / Open Access
• [Journal Article] RNA-Mediated Plant Behavior (2019)
  • Author(s): Ohtani Misato、Kurihara Yukio、Seki Motoaki、Crespi Martin
  • Journal Title: Plant and Cell Physiology Volume: 60 Issue: 9 Pages: 1893-1896
  • DOI: 10.1093/pcp/pcz168
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Recent advances in the characterization of plant transcriptomes in response to drought, salinity, heat and cold stress (2019)
  • Author(s): Bashir, K., Matsui, A., Rasheed, S. and Seki, M.
  • Journal Title: F1000 Faculty Reviews Volume: Rev-658 Pages: 658-658
  • DOI: 10.12688/f1000research.18424.1
  • Peer Reviewed / Open Access
• [Journal Article] Biological function of changes in RNA metabolism in plant adaptation to abiotic stress (2019)
  • Author(s): Matsui, A., Nakaminami, K. and Seki, M.
  • Journal Title: Plant Cell Physiol. Volume: 60 Issue: 9 Pages: 1897-1905
  • DOI: 10.1093/pcp/pcz068
  • Peer Reviewed
• [Journal Article] The involvement of long non-coding RNAs in response to plant stress (2019)
  • Author(s): Matsui, A.* and Seki, M.*
  • Journal Title: Methods in Molecular Biology Volume: 1933 Pages: 151-171
  • DOI: 10.1007/978-1-4939-9045-0_8
  • ISBN: 9781493990443, 9781493990450
  • Peer Reviewed
• [Journal Article] RNA Regulation in Plant Cold Stress Response (2018)
  • Author(s): Nakaminami Kentaro、Seki Motoaki
  • Journal Title: Adv Exp Med Biol Volume: 1081 Pages: 23-44
  • DOI: 10.1007/978-981-13-1244-1_2
  • ISBN: 9789811312434, 9789811312441
  • Peer Reviewed
• [Journal Article] AtPep3 is a hormone-like peptide that plays a role in the salinity stress tolerance of plants (2018)
  • Author(s): Nakaminami Kentaro、Okamoto Masanori、Higuchi-Takeuchi Mieko、Yoshizumi Takeshi、Yamaguchi Yube、Fukao Yoichiro、Shimizu Minami、Ohashi Chihiro、Tanaka Maho、Matsui Minami、Shinozaki Kazuo、Seki Motoaki、Hanada Kousuke
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 115 Issue: 22 Pages: 5810-5815
  • DOI: 10.1073/pnas.1719491115
  • Peer Reviewed
• [Journal Article] The modulation of acetic acid pathway genes in Arabidopsis produces drought-stress tolerant plants. (2018)
  • Author(s): Rasheed, S.#, Bashir, K.#, Kim, J.M., Ando, M., Tanaka, M. and Seki, M.
  • Journal Title: Scientific Rep. Volume: 8 Issue: 1 Pages: 7831-7831
  • DOI: 10.1038/s41598-018-26103-2
  • Peer Reviewed
• [Journal Article] Versatility of HDA19-deficiency for increasing tolerance to environmental stresses in Arabidopsis. (2018)
  • Author(s): Ueda, M., Matsui, A., Nakamura, T., Abe, T., Sunaoshi, Y., Shimada, H. and Seki, M.
  • Journal Title: Plant Signal. Behav. Volume: 13 Pages: 1-4
  • DOI: 10.1080/15592324.2018.1475808
  • Peer Reviewed
• [Journal Article] The duration of ethanol-induced high-salinity stress tolerance in Arabidopsis thaliana (2018)
  • Author(s): Sako Kaori、Sunaoshi Yuji、Tanaka Maho、Matsui Akihiro、Seki Motoaki
  • Journal Title: Plant Signaling & Behavior Volume: 13 Pages: 1-3
  • DOI: 10.1080/15592324.2018.1500065
  • Peer Reviewed
• [Presentation] Drought Resistant Plants and Growing Crops in the Desert (2020)
  • Author(s): Seki, M.
  • Organizer: The Scientific Session “Food of the Future: Developing New, Sustainable, and Healthy Sources”, The 2020 American Association for the Advancement of Science (AAAS) Annual Meeting
  • Int'l Joint Research / Invited
• [Presentation] Novel chemical regulation in plant abiotic stress adaptation and tolerance (2019)
  • Author(s): Seki, M.
  • Organizer: ICAR2019 Workshop “Plants Contribute to SDGs”, The 30th ICAR (ICAR2019)
  • Int'l Joint Research / Invited
• [Presentation] 植物の温度ストレス適応における転写後またはエピジェネティック制御機構の解析 (2019)
  • Author(s): 関原明、松井章浩、田中真帆、石田順子、上田実、砂押裕司、Thi Cam Chau Nguyen、Anh Hai Nguyen、中南健太郎
  • Organizer: 第19回日本蛋白質科学会年会・第71回日本細胞生物学会大会 合同年次大会共催シンポジウム“温度生物学：温度センシングと細胞機能”
  • Invited
• [Presentation] Novel chemical and epigenome regulation in plant abiotic stress adaptation (2019)
  • Author(s): Seki, M.
  • Organizer: Concurrent Session “Abiotic Interactions”, The 23rd International Plant Growth Substances Conference (IPGSA)
  • Int'l Joint Research / Invited
• [Presentation] 化学・ペプチド制御による植物の環境ストレス適応力強化 (2019)
  • Author(s): 関原明
  • Organizer: 日本バイオスティミュラント協議会 第2回講演会
  • Invited
• [Presentation] Novel epigenetic, chemical and RNA regulation in plant abiotic stress adaptation (2019)
  • Author(s): Seki, M.
  • Organizer: Workshop on "Plant Responses to Abiotic Stresses and Enbiromnental Signals" (Second Series), China Agricultural Univ.
  • Int'l Joint Research / Invited
• [Presentation] Epigenome and non-coding antisense RNA regulation in plant abiotic stress adaptation (2019)
  • Author(s): Seki, M.
  • Organizer: The 3rd meeting of the Plant Epigenetics Consortium in Japan
  • Invited
• [Presentation] Novel Epigenetic, RNA and Peptide Regulation in Plant Abiotic Stress Adaptation (2018)
  • Author(s): Seki, M.
  • Organizer: Seminar at Institute of Network Biology (INET), Helmholtz Zentrum Munchen
  • Invited
• [Presentation] Novel Epigenetic and RNA Regulation in Plant Abiotic Stress Adaptation (2018)
  • Author(s): Seki, M.
  • Organizer: The 5th International Conference “Plant Abiotic Stress Tolerance”, Vienna International Science Conferences and Events Association (VISCEA)
  • Int'l Joint Research / Invited
• [Presentation] Molecular mechanisms of drought tolerance (2018)
  • Author(s): Matsui, A., Bashir, K., Ueda, M., Kim, J.M., Nguyen, H.M., Sako, K., Sunaoshi, Y., Rasheed, S., Nakaminami, K., Ishida, J., Tanaka, M. and Seki, M.
  • Organizer: FEBS Workshop 2018 - Resurrection plants: Hope for crop drought tolerance (ReHOPE)
  • Int'l Joint Research / Invited
• [Remarks] 国立研究開発法人理化学研究所環境資源科学研究センター植物ゲノム発現研究チームホームページ
  • URL: http://www.csrs.riken.jp/jp/labs/pgnrt/index.html