ヒストン脱メチル化酵素JUMONJIによる高温を記憶する分子基盤の解明

• Project Area: Integrative system of autonomous environmental signal recognition and memorization for plant plasticity
• Project/Area Number: 18H04782
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: 山口 暢俊 奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (90767899)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000); Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: エピジェネティクス / 植物 / 高温順化 / ヒストン / JUMONJI / 植物生理 / 遺伝子発現 / 環境

Outline of Annual Research Achievements

1）高温順化におけるJMJの役割: 中程度高温によって順化をさせて、時間経過したのちに高温を与えた場合、野生型は高温順化して、耐性を保持した。しかし、jmj変異体では、高温順化する能力が低く、耐性を維持しにくくなることがわかった。また、順化前にJMJの活性を戻すと表現型は回復した。
2）JMJによる遺伝子発現の制御: RNA-seqによりJMJが発現を制御する遺伝子をゲノムワイドに同定し、順化によって発現するタイミングが早くなるHSP20-LIKE、HSP22遺伝子など複数のHSP遺伝子を同定した。
3）JMJによるヒストン修飾の制御: ChIP-seqにより、JMJが発現を制御する遺伝子をゲノムワイドに同定した。JMJが順化によって、HSP20-LIKE、HSP22遺伝子座のH3K27me3のマークを除去し、それがしばらく維持されることが記憶の分子実体であることがわかった。このヒストン修飾状態の数理モデルを構築して、HSP遺伝子の発現を予測可能にした。さらにhsp20-like hsp22は高温順化能が低いことを明らかにした。
4）変動温度環境下におけるJMJの役割: 緯度の異なる野外の変動する温度で野生型とjmj変異体を生育しても、変異体ではヒストン修飾を介したHSP遺伝子の発現変化により高温順化能が低下した。そのため、変動する環境下でもJMJが順化に必要である可能性が示唆された。

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Abscisic acid-dependent histone demethylation during postgermination growth arrest in Arabidopsis. (2019)
  • Author(s): Wu, J., Ichihashi, Y., Suzuki, T., Shibata, A., Shirasu, K., Yamaguchi, N. and Ito T.
  • Journal Title: Plant Cell and Environment Volume: 42 Pages: 2198-2214
  • Peer Reviewed
• [Journal Article] Histone demethylases control root elongation in response to stress-signaling hormone abscisic acid. (2019)
  • Author(s): Wu, J., Yamaguchi, N. and Ito T.
  • Journal Title: Plant Signaling and Behavior Volume: 14 Pages: 1604019-1604019
  • Peer Reviewed
• [Journal Article] When to stop: an update on molecular mechanisms of floral meristem termination. (2019)
  • Author(s): Xu Y., Yamaguchi, N. Gan, E-S., and Ito, T.
  • Journal Title: Journal of Experimental Botany Volume: 70 Pages: 1711-1718
  • Peer Reviewed
• [Journal Article] Integration of transcriptional repression and Polycomb-mediated silencing of WUSCHEL in floral meristems. (2019)
  • Author(s): Sun, B., Zhou, Y., Cai, J., Shang E., Yamaguchi, N., Xiao, J., Looi, L-S., Wee W-Y., Gao, X., Wagner, D., and Ito, T.
  • Journal Title: Plant Cell Volume: 31 Pages: 1488-1505
  • NAID: 120006726651
  • Peer Reviewed
• [Journal Article] The roles of plant hormones and their interactions with regulatory genes in determining meristem activity. (2019)
  • Author(s): Ze Hong, L., Hirakawa, T., Yamaguchi, N., Ito, T.
  • Journal Title: International Journals of Molecular Sciences Volume: 20 Pages: 4065-4065
  • Peer Reviewed
• [Journal Article] Lateral root initiation requires the sequential induction of transcription factors LBD16 and PUCHI in Arabidopsis thaliana. (2019)
  • Author(s): Goh, T., Toyokura, K., Yamaguchi, N., Okamoto, T., Uehara, T., Kaneko, S., Takebayashi, Y., Kasahara, H., Ikeyama, Y., Okushima, Y., Nakajima, K., Mimura, T., Tasaka, M., and Fukaki, H.
  • Journal Title: New Phytologist Volume: 222 Pages: 2-2
  • Peer Reviewed
• [Journal Article] CRABS CLAW and SUPERMAN coordinate hormone-, stress-, and metabolic-related gene expression during Arabidopsis stamen development. (2019)
  • Author(s): Ze Hong, L., Tatsumi, Y., Ichihashi, Y., Suzuki, T., Shibata, A., Shirasu, K., Yamaguchi, N. and Ito T.
  • Journal Title: Frontiers in Ecology and Evolution. Volume: 7 Pages: 437-437
  • Peer Reviewed
• [Journal Article] Abscisic acid‐dependent histone demethylation during postgermination growth arrest in Arabidopsis (2019)
  • Author(s): Wu Jinfeng、Ichihashi Yasunori、Suzuki Takamasa、Shibata Arisa、Shirasu Ken、Yamaguchi Nobutoshi、Ito Toshiro
  • Journal Title: Plant, Cell & Environment Volume: - Issue: 7 Pages: 2198-2214
  • DOI: 10.1111/pce.13547
  • Peer Reviewed / Open Access
• [Journal Article] Chromatin-mediated feed-forward auxin biosynthesis in floral meristem determinacy. (2018)
  • Author(s): Yamaguchi, N., Huang, J., Tatsumi, Y., Abe, M., Sugano, S. S., Kojima, M., Takebayashi, Y., Kiba, T., Yokoyama, R., Nishitani, K., Sakakibara, H., and Ito, T.
  • Journal Title: Nature Commun. Volume: 9 Issue: 1 Pages: 5290-5290
  • DOI: 10.1038/s41467-018-07763-0
  • NAID: 120006549567
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] SUPERMAN regulates floral whorl boundaries through control of auxin biosynthesis. (2018)
  • Author(s): Xu, Y., Prunet, N., Gan, E-S., Wang, Y., Stewart, D., Wellmer, F., Huang, J., Yamaguchi, N., Tatsumi, Y., Kojima, M., Kiba, T., Sakakibara, H., Jack, T.P., Meyerowitz, E.M., Ito, T.
  • Journal Title: EMBO Journal Volume: - Issue: 11
  • DOI: 10.15252/embj.201797499
  • NAID: 120006478564
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis (2018)
  • Author(s): Ze Hong Lee, Nobutoshi Yamaguchi, Toshiro Ito
  • Journal Title: Plant Transcription Factors.Methods in molecular biology (Clifton, N.J.) Volume: 1830 Pages: 93-108
  • DOI: 10.1007/978-1-4939-8657-6_6
  • ISBN: 9781493986569, 9781493986576
  • Peer Reviewed
• [Journal Article] Gene Expression and Transcription Factor Binding Tests Using Mutated-Promoter Reporter Lines (2018)
  • Author(s): Nishizawa-Yokoi Ayako、Yamaguchi Nobutoshi
  • Journal Title: Methods in molecular biology (Clifton, N.J.) Volume: 2 Pages: 291-305
  • DOI: 10.1007/978-1-4939-8657-6_17
  • ISBN: 9781493986569, 9781493986576
  • Peer Reviewed