アンチセンスncRNAを介した植物の環境ストレス認識・記憶システムの解析

• Project Area: Integrative system of autonomous environmental signal recognition and memorization for plant plasticity
• Project/Area Number: 18H04791
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: 関 原明 国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (80281624)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000); Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: アンチセンスRNA / 長鎖ncRNA / 植物の環境ストレス適応 / 環境ストレス記憶

Outline of Annual Research Achievements

1.ストレス誘導性のアンチセンスncRNAの生成に関わる新規因子の同定および機能解析：2本鎖RNA分解に関与する2本鎖RNaseのRTL1を同定し解析を進めた。RTL1をターゲットとするエストラジオール誘導性人工miRNAの形質転換体の作成を試みたが、野生株では人工miRNAのジーン・サイレンシングが起こり効果的に発現抑制ができなかった。最近の論文を参考にシーンサイレンシングを抑制するrdr6変異体バックグラウンドで形質転換体を作成し直している。また、本年度はrdr1/2/6変異体にXRN4をターゲットするエストラジオール誘導性人工miRNAの形質転換体を作成し表現型解析を進めた。エストラジオール処理無しでは、rdr1/2/6変異体は通常条件では根の伸長は野生株と同じであるのに対して、３時間浸透圧ストレス処理後３日目ではrdr1/2/6変異体において根の再成長が野生株よりも遅くなった。一方、エストラジオール処理有りでは通常条件でも浸透圧ストレス条件でもrdr1/2/6変異体において根の再成長が野生株よりも同等に遅くなった。この結果は、RDR1/2/6とXRN4が根の成長制御に相補的に機能し、浸透圧ストレス処理後の再成長には主にRDR1/2/6が機能する事を示唆する。今後、ターゲット遺伝子のmRNA発現とRNA分解速度を調べる予定である。
2．ストレス誘導性のアンチセンスncRNAの環境ストレス適応・記憶における生理的な機能解明：前年度のトランスクリプト―ム解析などから、RDR1/2/6はSA応答の一部を抑制する機能を有することが示唆された。浸透圧ストレス処理後の回復過程で、rdr1/2/6変異体において4つのWRKY転写因子の遺伝子発現が、野生株と比較して上昇していた。これらWRKY遺伝子のゲノム編集系統を作成し、解析を進めている。

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] The dynamic kaleidoscope of RNAs in plants (2020)
  • Author(s): Bailey-Serres, J., Zhai, J. and Seki, M.
  • Journal Title: Plant Physiol. Volume: 182 Issue: 1 Pages: 1-9
  • DOI: 10.1104/pp.19.01558
  • Open Access / Int'l Joint Research
• [Journal Article] Histone modifications form epigenetic regulatory networks to regulate abiotic stress response (2020)
  • Author(s): Ueda, M. and Seki, M.
  • Journal Title: Plant Physiol. Volume: 182 Issue: 1 Pages: 15-26
  • DOI: 10.1104/pp.19.00988
  • Peer Reviewed / Open Access
• [Journal Article] Transcriptome analysis of the hierarchical response of HDAC proteins that respond in an antagonistic manner to salinity stress. (2019)
  • Author(s): Ueda, M., Matsui, A., Watanabe, S., Kobayashi, M., Saito, K., Tanaka, M., Ishida, J., Kusano, M., Seo, M. and Seki, M.
  • Journal Title: Front. Plant Sci. Volume: 10 Pages: 1323-1323
  • DOI: 10.3389/fpls.2019.01323
  • Peer Reviewed / Open Access
• [Journal Article] RNA-Mediated Plant Behavior (2019)
  • Author(s): Ohtani Misato、Kurihara Yukio、Seki Motoaki、Crespi Martin
  • Journal Title: Plant and Cell Physiology Volume: 60 Issue: 9 Pages: 1893-1896
  • DOI: 10.1093/pcp/pcz168
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Recent advances in the characterization of plant transcriptomes in response to drought, salinity, heat and cold stress (2019)
  • Author(s): Bashir, K., Matsui, A., Rasheed, S. and Seki, M.
  • Journal Title: F1000 Faculty Reviews Volume: Rev-658 Pages: 658-658
  • DOI: 10.12688/f1000research.18424.1
  • Peer Reviewed / Open Access
• [Journal Article] Biological function of changes in RNA metabolism in plant adaptation to abiotic stress (2019)
  • Author(s): Matsui, A., Nakaminami, K. and Seki, M.
  • Journal Title: Plant Cell Physiol. Volume: 60 Issue: 9 Pages: 1897-1905
  • DOI: 10.1093/pcp/pcz068
  • Peer Reviewed
• [Journal Article] The involvement of long non-coding RNAs in response to plant stress (2019)
  • Author(s): Matsui, A.* and Seki, M.*
  • Journal Title: Methods in Molecular Biology Volume: 1933 Pages: 151-171
  • DOI: 10.1007/978-1-4939-9045-0_8
  • ISBN: 9781493990443, 9781493990450
  • Peer Reviewed
• [Journal Article] The duration of ethanol-induced high-salinity stress tolerance in Arabidopsis thaliana (2018)
  • Author(s): Sako Kaori、Sunaoshi Yuji、Tanaka Maho、Matsui Akihiro、Seki Motoaki
  • Journal Title: Plant Signaling & Behavior Volume: 13 Pages: 1-3
  • DOI: 10.1080/15592324.2018.1500065
  • Peer Reviewed
• [Journal Article] Versatility of HDA19-deficiency for increasing tolerance to environmental stresses in Arabidopsis. (2018)
  • Author(s): Ueda, M., Matsui, A., Nakamura, T., Abe, T., Sunaoshi, Y., Shimada, H. and Seki, M.
  • Journal Title: Plant Signal. Behav. Volume: 13 Pages: 1-4
  • DOI: 10.1080/15592324.2018.1475808
  • Peer Reviewed
• [Journal Article] AtPep3 is a hormone-like peptide that plays a role in the salinity stress tolerance of plants (2018)
  • Author(s): Nakaminami Kentaro、Okamoto Masanori、Higuchi-Takeuchi Mieko、Yoshizumi Takeshi、Yamaguchi Yube、Fukao Yoichiro、Shimizu Minami、Ohashi Chihiro、Tanaka Maho、Matsui Minami、Shinozaki Kazuo、Seki Motoaki、Hanada Kousuke
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 115 Issue: 22 Pages: 5810-5815
  • DOI: 10.1073/pnas.1719491115
  • Peer Reviewed
• [Journal Article] The modulation of acetic acid pathway genes in Arabidopsis produces drought-stress tolerant plants. (2018)
  • Author(s): Rasheed, S.#, Bashir, K.#, Kim, J.M., Ando, M., Tanaka, M. and Seki, M.
  • Journal Title: Scientific Rep. Volume: 8 Issue: 1 Pages: 7831-7831
  • DOI: 10.1038/s41598-018-26103-2
  • Peer Reviewed
• [Journal Article] Transcriptomic analysis of Arabidopsis thaliana plants treated with the Ky-9 and Ky-72 histone deacetylase inhibitors (2018)
  • Author(s): Nguyen HM, Sako K, Matsui A, Ueda M, Tanaka M, Ito A, Nishino N, Yoshida M, Seki M.
  • Journal Title: Plant Signaling and Behavior Volume: 13 Issue: 3 Pages: e1448333-e1448333
  • DOI: 10.1080/15592324.2018.1448333
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Drought Resistant Plants and Growing Crops in the Desert (2020)
  • Author(s): Seki, M.
  • Organizer: The Scientific Session “Food of the Future: Developing New, Sustainable, and Healthy Sources”, The 2020 American Association for the Advancement of Science (AAAS) Annual Meeting
  • Int'l Joint Research / Invited
• [Presentation] Novel chemical regulation in plant abiotic stress adaptation and tolerance (2019)
  • Author(s): Seki, M.
  • Organizer: ICAR2019 Workshop “Plants Contribute to SDGs”, The 30th ICAR (ICAR2019)
  • Int'l Joint Research / Invited
• [Presentation] 植物の温度ストレス適応における転写後またはエピジェネティック制御機構の解析 (2019)
  • Author(s): 関原明、松井章浩、田中真帆、石田順子、上田実、砂押裕司、Thi Cam Chau Nguyen、Anh Hai Nguyen、中南健太郎
  • Organizer: 第19回日本蛋白質科学会年会・第71回日本細胞生物学会大会 合同年次大会共催シンポジウム“温度生物学：温度センシングと細胞機能”
  • Invited
• [Presentation] Novel chemical and epigenome regulation in plant abiotic stress adaptation (2019)
  • Author(s): Seki, M.
  • Organizer: Concurrent Session “Abiotic Interactions”, The 23rd International Plant Growth Substances Conference (IPGSA)
  • Int'l Joint Research / Invited
• [Presentation] 化学・ペプチド制御による植物の環境ストレス適応力強化 (2019)
  • Author(s): 関原明
  • Organizer: 日本バイオスティミュラント協議会 第2回講演会
  • Invited
• [Presentation] Novel epigenetic, chemical and RNA regulation in plant abiotic stress adaptation (2019)
  • Author(s): Seki, M.
  • Organizer: Workshop on "Plant Responses to Abiotic Stresses and Enbiromnental Signals" (Second Series), China Agricultural Univ.
  • Int'l Joint Research / Invited
• [Presentation] Epigenome and non-coding antisense RNA regulation in plant abiotic stress adaptation (2019)
  • Author(s): Seki, M.
  • Organizer: The 3rd meeting of the Plant Epigenetics Consortium in Japan
  • Invited
• [Presentation] Novel antisense RNA and epigenome regulation in plant abiotic stress adaptation (2019)
  • Author(s): Matsui, A., Ueda, M., Bashir, K., Sako, K., Kim, J.M., Nguyen, H.M., Sunaoshi, Y., Rasheed, S., Ishida, J., Tanaka, M. and Seki, M.
  • Organizer: The International Conference on Post-transcriptional Gene Regulation in Plants (PGRP) 2019
  • Int'l Joint Research / Invited
• [Presentation] Novel Epigenetic, RNA and Peptide Regulation in Plant Abiotic Stress Adaptation (2018)
  • Author(s): Seki, M.
  • Organizer: Seminar at Institute of Network Biology (INET), Helmholtz Zentrum Munchen
  • Invited
• [Presentation] Novel Epigenetic and RNA Regulation in Plant Abiotic Stress Adaptation (2018)
  • Author(s): Seki, M.
  • Organizer: The 5th International Conference “Plant Abiotic Stress Tolerance”, Vienna International Science Conferences and Events Association (VISCEA)
  • Int'l Joint Research / Invited
• [Presentation] Molecular mechanisms of drought tolerance (2018)
  • Author(s): Matsui, A., Bashir, K., Ueda, M., Kim, J.M., Nguyen, H.M., Sako, K., Sunaoshi, Y., Rasheed, S., Nakaminami, K., Ishida, J., Tanaka, M. and Seki, M.
  • Organizer: FEBS Workshop 2018 - Resurrection plants: Hope for crop drought tolerance (ReHOPE)
  • Int'l Joint Research / Invited
• [Remarks] 国立研究開発法人理化学研究所環境資源科学研究センター植物ゲノム発現研究チームホームページ
  • URL: http://www.csrs.riken.jp/jp/labs/pgnrt/index.html