空間トランスオミクス技術の開発

• Project Area: Transomic Analysis of Metabolic Adaptation
• Project/Area Number: 18H04802
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Research Institution: Kyushu University
• Principal Investigator: 大川 恭行 九州大学, 生体防御医学研究所, 教授 (80448430)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000); Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: エピゲノム / オミクス / トランスクリプトーム / ローカライゾーム / トランスオミクス / プロテオーム / クロマチン / トランスクリプトミクス / 組織解析

Outline of Annual Research Achievements

独自に開発した1細胞レベル且つ固定化した細胞・組織切片上で解析可能な新たなクロマチン構造解析技術ChILseq法(Nat Cell Biol. 2018：責任著者)を更に発展させ、トランスクリプトームを同時解析する手法の確立を目指した。具体的には独自に開発した1細胞レベル且つ固定化した細胞・組織切片上で解析可能な新たなクロマチン構造解析技術ChILseq法(Nat Cell Biol. 2018：責任著者)を更に発展させ、トランスクリプトームを同時解析する手法の確立を行った。更に、トランスクリプトーム、エピゲノム、プロテオームの３つの情報を加えた空間トランスオミクス技術の開発を行った。
エピゲノム情報は、組織切片を予めChILT法、プロテオーム情報はCITEseqと同様のアプローチながらChILseq用プローブを発展的に改変した手法によりデータ取得を試みた。エピゲノム情報をpolyARNAに置換させておき、トランスクリプトーム取得時に同時に獲得に成功した。本法はChILseq2.0と命名し、予備検討に成功、現在論文発表準備を進めている。一方で、エピゲノム、プロテオーム情報を取得する手法については現在公開準備を進めている。またプロテオーム解析については、用いる抗体の数がボトルネックとなるため、現在利用可能な抗体数を最大化する新たなモノクローナル抗体開発法の確立を進めた（論文発表準備中）。また、空間オミクスは、１分子FISHを取り入れた新たな解析法の確立を進めた。ChILseq2.0については実データ取得を既に行い現在共同研究を進めている。

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] The role of galanin in the differentiation of mucosal mast cells in mice. (2020)
  • Author(s): Yamaguchi T., Ikeda Y., Tashiro K., Ohkawa Y., Kawabata K.
  • Journal Title: Eur. J. Immunol. Volume: 50 Issue: 1 Pages: 110-118
  • DOI: 10.1002/eji.201848061
  • Peer Reviewed / Open Access
• [Journal Article] Chromatin-bound CRM1 recruits SET-Nup214 and NPM1c onto HOX clusters causing aberrant HOX expression in leukemia cells. (2019)
  • Author(s): Oka M, Mura S, Otani M, Miyamoto Y, Nogami J, Maehara K, Harada A, Tachibana T, Yoneda Y, Ohkawa Y
  • Journal Title: Elife Volume: 8
  • DOI: 10.7554/elife.46667
  • Peer Reviewed / Open Access
• [Journal Article] Cell competition corrects noisy Wnt morphogen gradients to achieve robust patterning in the zebrafish embryo (2019)
  • Author(s): Akieda Yuki、Ogamino Shohei、Furuie Hironobu、Ishitani Shizuka、Akiyoshi Ryutaro、Nogami Jumpei、Masuda Takamasa、Shimizu Nobuyuki、Ohkawa Yasuyuki、Ishitani Tohru
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 4710-4710
  • DOI: 10.1038/s41467-019-12609-4
  • Peer Reviewed / Open Access
• [Journal Article] Sustained expression of HeyL is critical for the proliferation of muscle stem cells in overloaded muscle. (2019)
  • Author(s): Fukuda S, Kaneshige A, Kaji T, Noguchi YT, Takemoto Y, Zhang L, Tsujikawa K, Kokubo H, Uezumi A, Maehara K, Harada A, Ohkawa Y, Fukada SI.
  • Journal Title: eLife Volume: 8
  • DOI: 10.7554/elife.48284
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Calcineurin Broadly Regulates the Initiation of Skeletal Muscle-Specific Gene Expression by Binding Target Promoters and Facilitating the Interaction of the SWI/SNF Chromatin Remodeling Enzyme. (2019)
  • Author(s): Witwicka H, Nogami J, Syed SA, Maehara K, Padilla-Benavides T, Ohkawa Y, Imbalzano AN
  • Journal Title: Mol Cell Biol. Volume: 39 Issue: 19
  • DOI: 10.1128/mcb.00063-19
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Eleanor ncRNAs activate the topological domain of the ESR1 locus to balance against apoptosis. (2019)
  • Author(s): †Abdalla MOA, †Yamamoto T, Maehara K, Ohkawa Y, Miura H, Hiratani I, Nakayama H, Nakao M, *Saitoh N
  • Journal Title: Nat Commun Volume: 10 Issue: 1 Pages: 3778-3778
  • DOI: 10.1038/s41467-019-11378-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Biochemical analysis of nucleosome targeting by Tn5 transposase (2019)
  • Author(s): Sato S, Arimura Y, Kujirai T, Harada A, Maehara K, Nogami J, Ohkawa Y, Kurumizaka H.
  • Journal Title: Open Biol. Volume: 9 Issue: 8 Pages: 190116-190116
  • DOI: 10.1098/rsob.190116
  • Peer Reviewed / Open Access
• [Journal Article] Dmrt factors determine the positional information of cerebral cortical progenitors via differential suppression of homeobox genes. (2019)
  • Author(s): Konno D, Kishida C, Maehara K, Ohkawa Y, Kiyonari H, Okada S, Matsuzaki F
  • Journal Title: Development Volume: 146 Issue: 15 Pages: 174243-174243
  • DOI: 10.1242/dev.174243
  • Peer Reviewed / Open Access
• [Journal Article] Anti-tumour effects of antimicrobial peptides, components of the innate immune system, against haematopoietic tumours in Drosophila mxc mutants. (2019)
  • Author(s): Araki M, Kurihara M, Kinoshita S, Awane R, Sato T, Ohkawa Y, Inoue YH
  • Journal Title: Dis Model Mech. Volume: 12 Issue: 6
  • DOI: 10.1242/dmm.037721
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of ectopic heterochromatin-mediated epigenetic diversification by the JmjC family protein Epe1. (2019)
  • Author(s): Sorida M, Hirauchi T, Ishizaki H, Kaito W, Shimada A, Mori C, Chikashige Y, Hiraoka Y, Suzuki Y, Ohkawa Y, Kato H, Takahata S, Murakami Y
  • Journal Title: PLoS Genet. Volume: 15
  • NAID: 120007002166
  • Peer Reviewed / Open Access
• [Journal Article] CLEC3A, MMP7, and LCN2 as Novel Markers for Predicting Recurrence in Resected G1 and G2 Pancreatic Neuroendocrine Tumors (2019)
  • Author(s): Miki M, Oono T, Fujimori N, Takaoka T, Kawabe K, Miyasaka Y, Ohtsuka T, Saito D, Nakamura M, Ohkawa Y, Oda N, Suyama M, Ito T, Ogawa Y.
  • Journal Title: Cancer Medicine Volume: in press Issue: 8 Pages: 3748-3760
  • DOI: 10.1002/cam4.2232
  • Peer Reviewed / Open Access
• [Journal Article] Macrophage centripetal migration drives spontaneous healing process after spinal cord injury (2019)
  • Author(s): Kobayakawa K、Ohkawa Y、 Yoshizaki S、T、Saito T、Kijima Ken、Yokota K、Hara M、Kubota K、Matsumoto Y、Harimaya K、Ozato K、Masuda T、Tsuda M、Tamura T、Inoue K、Edgerton V. R、Iwamoto Y、Nakashima Y、Okada S
  • Journal Title: Science Advances Volume: 5 Issue: 5
  • DOI: 10.1126/sciadv.aav5086
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Locomotor Training Increases Synaptic Structure With High NGL-2 Expression After Spinal Cord Hemisection (2019)
  • Author(s): Kobayakawa Kazu、DePetro Kyleigh Alexis、Zhong Hui、Pham Bau、Hara Masamitsu、Harada Akihito、Nogami Jumpei、Ohkawa Yasuyuki、Edgerton V. Reggie
  • Journal Title: Neurorehabilitation and Neural Repair Volume: 33 Issue: 3 Pages: 225-231
  • DOI: 10.1177/1545968319829456
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] miR-124 dosage regulates prefrontal cortex function by dopaminergic modulation. (2019)
  • Author(s): Kozuka T, Omori Y, Watanabe S, Tarusawa E, Yamamoto H, Chaya T, Furuhashi M, Morita M, Sato T, Hirose S, Ohkawa Y, Yoshimura Y, Hikida T, Furukawa T.
  • Journal Title: Sci Rep. Volume: Mar 5;9(1) Issue: 1 Pages: 3445-3445
  • DOI: 10.1038/s41598-019-38910-2
  • NAID: 120006579467
  • Peer Reviewed / Open Access
• [Journal Article] Cell-autonomous and redundant roles of Hey1 and HeyL in muscle stem cells: HeyL requires Hes1 to bind diverse DNA sites (2019)
  • Author(s): Noguchi Yu-taro、Nakamura Miki、Hino Nobumasa、Nogami Jumpei、Tsuji Sayaka、Sato Takahiko、Zhang Lidan、Tsujikawa Kazutake、Tanaka Toru、Izawa Kohei、Okada Yoshiaki、Doi Takefumi、Kokubo Hiroki、Harada Akihito、Uezumi Akiyoshi、Gessler Manfred、Ohkawa Yasuyuki、Fukada So-ichiro
  • Journal Title: Development Volume: 146 Issue: 4 Pages: 163618-163618
  • DOI: 10.1242/dev.163618
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pathological changes of distal motor neurons after complete spinal cord injury (2019)
  • Author(s): Yokota Kazuya、Kubota Kensuke、Kobayakawa Kazu、Saito Takeyuki、Hara Masamitsu、Kijima Ken、Maeda Takeshi、Katoh Hiroyuki、Ohkawa Yasuyuki、Nakashima Yasuharu、Okada Seiji
  • Journal Title: Molecular Brain Volume: 12 Issue: 1
  • DOI: 10.1186/s13041-018-0422-3
  • Peer Reviewed
• [Journal Article] Transcriptome profiling of refractory atopic keratoconjunctivitis by RNA sequencing (2019)
  • Author(s): Matsuda Akira、Asada Yosuke、Suita Naomasa、Iwamoto Satoshi、Hirakata Toshiaki、Yokoi Norihiko、Ohkawa Yasuyuki、Okada Yukinori、Yokomizo Takehiko、Ebihara Nobuyuki
  • Journal Title: Journal of Allergy and Clinical Immunology Volume: 143 Issue: 4 Pages: 1610-1614
  • DOI: 10.1016/j.jaci.2018.11.007
  • Peer Reviewed / Open Access
• [Journal Article] Mouse polycomb group gene Cbx2 promotes osteoblastic but suppresses adipogenic differentiation in postnatal long bones (2019)
  • Author(s): Katoh-Fukui Yuko、Baba Takashi、Sato Tetsuya、Otake Hiroyuki、Nagakui-Noguchi Yuko、Shindo Miyuki、Suyama Mikita、Ohkawa Yasuyuki、Tsumura Hideki、Morohashi Ken-ichirou、Fukami Maki
  • Journal Title: Bone Volume: 120 Pages: 219-231
  • DOI: 10.1016/j.bone.2018.10.021
  • Peer Reviewed / Open Access
• [Journal Article] A chromatin integration labelling method enables epigenomic profiling with lower input (2018)
  • Author(s): Harada Akihito、Maehara Kazumitsu、Handa Tetsuya、Arimura Yasuhiro、Nogami Jumpei、Hayashi-Takanaka Yoko、Shirahige Katsuhiko、Kurumizaka Hitoshi、Kimura Hiroshi、Ohkawa Yasuyuki
  • Journal Title: Nature Cell Biology Volume: 21 Issue: 2 Pages: 287-296
  • DOI: 10.1038/s41556-018-0248-3
  • Peer Reviewed / Open Access
• [Journal Article] Fetal Leydig cells dedifferentiate and serve as adult Leydig stem cells (2018)
  • Author(s): Shima Yuichi、Miyabayashi Kanako、Sato Tetsuya、Suyama Mikita、Ohkawa Yasuyuki、Doi Masao、Okamura Hitoshi、Suzuki Kentaro
  • Journal Title: Development Volume: 145 Issue: 23 Pages: 169136-169136
  • DOI: 10.1242/dev.169136
  • Peer Reviewed
• [Journal Article] Prolonged inhibition of hepatocellular carcinoma cell proliferation by combinatorial expression of defined transcription factors (2018)
  • Author(s): Takashima Yasuo、Horisawa Kenichi、Udono Miyako、Ohkawa Yasuyuki、Suzuki Atsushi
  • Journal Title: Cancer Science Volume: 109 Issue: 11 Pages: 3543-3553
  • DOI: 10.1111/cas.13798
  • Peer Reviewed / Open Access
• [Journal Article] Cancer-associated mutations of histones H2B, H3.1 and H2A.Z.1 affect the structure and stability of the nucleosome (2018)
  • Author(s): Arimura Yasuhiro、Ikura Masae、Fujita Risa、Noda Mamiko、Kobayashi Wataru、Horikoshi Naoki、Sun Jiying、Shi Lin、Kusakabe Masayuki、Harata Masahiko、Ohkawa Yasuyuki、Tashiro Satoshi、Kimura Hiroshi、Ikura Tsuyoshi、Kurumizaka Hitoshi
  • Journal Title: Nucleic Acids Research Volume: - Pages: 10007-10018
  • DOI: 10.1093/nar/gky661
  • NAID: 120006545208
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] MNase, as a probe to study the sequence-dependent site exposures in the +1 nucleosomes of yeast (2018)
  • Author(s): Luo Di、Kato Daiki、Nogami Jumpei、Ohkawa Yasuyuki、Kurumizaka Hitoshi、Kono Hidetoshi
  • Journal Title: Nucleic Acids Research Volume: 46 Issue: 14 Pages: 7124-7137
  • DOI: 10.1093/nar/gky502
  • Peer Reviewed / Open Access
• [Journal Article] Genome-wide analysis of the spatiotemporal regulation of firing and dormant replication origins in human cells (2018)
  • Author(s): Sugimoto Nozomi、Maehara Kazumitsu、Yoshida Kazumasa、Ohkawa Yasuyuki、Fujita Masatoshi
  • Journal Title: Nucleic Acids Research Volume: 46 Issue: 13 Pages: 6683-6696
  • DOI: 10.1093/nar/gky476
  • Peer Reviewed / Open Access
• [Journal Article] Identification of miR-305, a microRNA that promotes aging, and its target mRNAs in Drosophila (2018)
  • Author(s): Ueda Makiko、Sato Tetsuya、Ohkawa Yasuyuki、Inoue Yoshihiro H.
  • Journal Title: Genes to Cells Volume: 23 Issue: 2 Pages: 80-93
  • DOI: 10.1111/gtc.12555
  • Peer Reviewed / Open Access
• [Journal Article] Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration (2018)
  • Author(s): Harada Akihito、Maehara Kazumitsu、Ono Yusuke、Taguchi Hiroyuki、Yoshioka Kiyoshi、Kitajima Yasuo、Xie Yan、Sato Yuko、Iwasaki Takeshi、Nogami Jumpei、Okada Seiji、Komatsu Tetsuro、Semba Yuichiro、Takemoto Tatsuya、Kimura Hiroshi、Kurumizaka Hitoshi、Ohkawa Yasuyuki
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 1400-1400
  • DOI: 10.1038/s41467-018-03845-1
  • NAID: 120006949474
  • Peer Reviewed / Open Access
• [Journal Article] Ad4BP/SF-1 regulates cholesterol synthesis to boost the production of steroids (2018)
  • Author(s): Takashi Baba, Hiroyuki Otake, Miki Inoue, Tetsuya Sato, Yasuhiro Ishihara, Ju-Yeon Moon, Megumi Tsuchiya, Kanako Miyabayashi, Hidesato Ogawa, Yuichi Shima, Lixiang Wang, Ryuichiro Sato, Takeshi Yamazaki, Mikita Suyama, Masatoshi Nomura, Man Ho Choi, Yasuyuki Ohkawa & Ken-ichirou Morohashi
  • Journal Title: Communications Biology Volume: 1 Issue: 1 Pages: 1-9
  • DOI: 10.1038/s42003-018-0020-z
  • NAID: 120006799378
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sensitive detection of fluorescence in western blotting by merging images (2018)
  • Author(s): Kondo Yukari、Higa Shinichiro、Iwasaki Takeshi、Matsumoto Tomoya、Maehara Kazumitsu、Harada Akihito、Baba Yoshihiro、Fujita Masatoshi、Ohkawa Yasuyuki
  • Journal Title: PLOS ONE Volume: 13 Issue: 1 Pages: 0191532-0191532
  • DOI: 10.1371/journal.pone.0191532
  • Peer Reviewed / Open Access
• [Journal Article] The Autism-Related Protein CHD8 Cooperates with C/EBPβ to Regulate Adipogenesis (2018)
  • Author(s): Kita Yasuyuki、Katayama Yuta、Shiraishi Taichi、Oka Takeru、Sato Tetsuya、Suyama Mikita、Ohkawa Yasuyuki、Miyata Keishi、Oike Yuichi、Shirane Michiko、*Nishiyama Masaaki、*Nakayama Keiichi I.
  • Journal Title: Cell Reports Volume: 23 Issue: 7 Pages: 1988-2000
  • DOI: 10.1016/j.celrep.2018.04.050
  • Peer Reviewed / Open Access
• [Journal Article] Cryo-EM structure of the nucleosome containing the ALB1 enhancer DNA sequence (2018)
  • Author(s): Takizawa Yoshimasa、Tanaka Hiroki、Machida Shinichi、Koyama Masako、Maehara Kazumitsu、Ohkawa Yasuyuki、Wade Paul A.、Wolf Matthias、Kurumizaka Hitoshi
  • Journal Title: Open Biology Volume: 8 Issue: 3 Pages: 170255-170255
  • DOI: 10.1098/rsob.170255
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Direct Reprogramming of Spiral Ganglion Non-neuronal Cells into Neurons: Toward Ameliorating Sensorineural Hearing Loss by Gene Therapy (2018)
  • Author(s): Noda T, Meas SJ, Nogami J, Amemiya Y, Uchi R, Ohkawa Y, Nishimura K, Dabdoub A
  • Journal Title: Front Cell Dev Biol Volume: 6:16 Pages: 1-14
  • DOI: 10.3389/fcell.2018.00016
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 遺伝子の発現されやすさはどのように決まるのか？～クロマチンが規定する遺伝子発現制御能力～ (2019)
  • Author(s): 大川恭行
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] Development of chromatin integration labeling technology for single cell multiomics (2019)
  • Author(s): Yasuyuki Ohkawa
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] Spatial epigenomics approached by chromatin integration labeling technology (2019)
  • Author(s): 大川恭行
  • Organizer: Trans-Omics for Advanced Medical Sciences
• [Presentation] クロマチン挿入法による単一細胞エピゲノム解析 (2019)
  • Author(s): 大川恭行
  • Organizer: 日本人類遺伝学会第64回大会
• [Presentation] Chromatin integration labelling Technology for expanding multi-omics (2019)
  • Author(s): Yasuyuki Ohkawa
  • Organizer: EMBO Symposia: Multi-Omics
• [Presentation] Chromatin integration labelling technology for expanding multi-omics (2019)
  • Author(s): 大川恭行
  • Organizer: 第13回国際ゲノム会議
• [Presentation] 細胞ポテンシャル測定システムの開発 (2019)
  • Author(s): 大川恭行
  • Organizer: CREST領域会議
• [Presentation] 1細胞トランスクリプトミクス による骨格筋特異的クロマチン構造の解明 発表 (2019)
  • Author(s): 大川恭行
  • Organizer: ゲノム編集班会議
• [Presentation] Transcriptional Plasticity regulated by novel Histone H3 Varigants (2019)
  • Author(s): 大川恭行
  • Organizer: 理研連携プロジェクト「エピゲノム操作」
  • Invited
• [Presentation] Chromatin Integration Labeling- an Epigenomic Profiling for Single Cell Analysis (2019)
  • Author(s): Yasuyuki Ohkawa
  • Organizer: Cell Symposium: Single Cells: Technology to Biology
  • Int'l Joint Research
• [Presentation] 骨格筋再生におけるヒストン転換 (2019)
  • Author(s): 大川恭行
  • Organizer: 第７回骨格筋生物学研究会
• [Presentation] 単一細胞レベルのエピゲノム解析技術 (2019)
  • Author(s): 大川恭行
  • Organizer: 九大発産・学・官交流促進シーズ発表会2019
• [Presentation] Low-input Epigenomic Profiling Technology toward Identifying Myogenic Chomatin Structure. (2018)
  • Author(s): Yasuyuki Ohkawa
  • Organizer: Muscle Development, Regeneration and Disease 2018
  • Int'l Joint Research
• [Presentation] 遺伝子発現能から迫る骨格筋再生機構の解明～トランスクリプトミクスによるアプローチ～ (2018)
  • Author(s): 大川恭行
  • Organizer: NCNP神経研究所セミナー
  • Invited
• [Presentation] 1細胞エピゲノム解析技術の開発 (2018)
  • Author(s): 大川恭行
  • Organizer: 第12回日本エピジェネティクス研究会年会
• [Presentation] ゲノムワイドな遺伝子発現制御機構を基盤とした神経組織特異的クロマチン構造の解明 (2018)
  • Author(s): 大川恭行
  • Organizer: 【ゲノム編集】キックオフミーティング
• [Presentation] ChiLT-an epigenomic Profiling for Single Cell Analysis (2018)
  • Author(s): 大川恭行
  • Organizer: 第2回代謝統合オミクス領域会議
• [Presentation] クロマチン組成変化から考える細胞分化能制御～トランスクリプトミクスの新たな展開～ (2018)
  • Author(s): 大川恭行
  • Organizer: 東京大学理学部研究セミナー
  • Invited
• [Presentation] ChiLT-an Epigenomic Profiling for Single Cell Analysis (2018)
  • Author(s): Yasuyuki Ohkawa
  • Organizer: Gordon Research Conference「Chromatin Encounters Shaping Genome Architecture and Function」
  • Int'l Joint Research
• [Presentation] Histone H3.3 sub-variants are required for regulating skeletal muscle differentiation. (2018)
  • Author(s): Yasuyuki Ohkawa
  • Organizer: 2018 Federation of American Societies for Experimental Biology (FASEB)
  • Int'l Joint Research
• [Presentation] Histone composition in chromatin regulates skeletal muscle regeneration (2018)
  • Author(s): 大川恭行
  • Organizer: The 6th Society of Skeletal Muscle Cells
• [Book] 実験医学 (2019)
  • Author(s): 原田 哲仁,大川 恭行
  • Total Pages: 6
  • Publisher: 羊土社
• [Patent(Industrial Property Rights)] 対象核酸の塩基配列を１細胞レベルで並列に検出する方法 (2020)
  • Inventor(s): 大川 恭行、原田哲仁
  • Industrial Property Rights Holder: 国立大学法人九州大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2020