花幹細胞の終結過程における遺伝子ネットワークの冗長性と協調性

• Project Area: Principles of pluripotent stem cells underlying plant vitality
• Project/Area Number: 18H04839
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: 伊藤 寿朗 奈良先端科学技術大学院大学, 先端科学技術研究科, 教授 (90517096)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000); Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 発現制御 / 幹細胞 / 増殖抑制 / 発生・分化 / シグナル伝達 / ホルモンシグナル

Outline of Annual Research Achievements

本研究では、花において旺盛な幹細胞の増殖活性を自ら停止し、生殖器官を分化させる仕組みを明らかにするために、これまでの単一変異の解析では見落とされていた遺伝子機能を多重変異体の活用により分子遺伝学的に解明することを主目的としている。シロイヌナズナにおいて、独立に花幹細胞を抑制すると考えられている３つの経路を構成する主要因子：AGAMOUS (AG)、SUPERMAN (SUP)の2つの転写因子と、CLAVATA3 (CLV3) ペプチドリガンドの遺伝学的な相互作用やSUPの作用機構はすでに論文として報告した(Plant Reproduction, 2018; EMBO J. 2018)。さらにCRCによるクロマチンを介したオーキシン合成酵素の制御機構の論文をNature Communications (2018)にて発表した。そこで、我々は、細胞非自立的に働くSUP, CRCタンパク質によるオーキシンシグナルの制御を介した花幹細胞の増殖抑制機構について、特に、2つの転写因子であるSUPとCRCの遺伝学的な相互作用について、二重突然変異体をもちいたオミクス解析の報告を行った。sup crc二重突然変異体は花幹細胞の異常増殖が花発生初期より亢進しており、オーキシン下流において、細胞壁組成やサイトカイニンシグナルの他、代謝経路、ストレス経路が大きく変動していることを明らかにした (Front. Ecol. Evol.2019)。これらSUPとCRCで共通して制御される遺伝子によって、花幹細胞の増殖抑制および正常な数の雄しべ形成がもたらされていることがわかった。
さらに、花幹細胞の増殖抑制時のレビューをInter J. of Mol Sci, 2019やPlant Sig. ＆ Behav. 2019に発表した。

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] CRABS CLAW and SUPERMAN coordinate hormone-, stress- and metabolic-related gene expression during Arabidopsis stamen development (2019)
  • Author(s): Lee, H. Z., Tatsumi, Y. Ichihashi, Y., Suzuki, T., Shibata, A., Shirasu, K., Yamaguchi, N., Ito, T.
  • Journal Title: Front Plant Sci. Volume: 7 Pages: 437-437
  • DOI: 10.3389/fevo.2019.00437
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Functional Dissection of Class C Genes in Rice (2019)
  • Author(s): Ito Toshiro
  • Journal Title: Plant and Cell Physiology Volume: 60 Issue: 6 Pages: 1164-1165
  • DOI: 10.1093/pcp/pcz093
  • NAID: 120007123945
  • Peer Reviewed
• [Journal Article] The Roles of Plant Hormones and Their Interactions with Regulatory Genes in Determining Meristem Activity (2019)
  • Author(s): Lee Ze Hong、Hirakawa Takeshi、Yamaguchi Nobutoshi、Ito Toshiro
  • Journal Title: International Journal of Molecular Sciences Volume: 20 Issue: 16 Pages: 4065-4065
  • DOI: 10.3390/ijms20164065
  • Peer Reviewed / Open Access
• [Journal Article] Control of floral stem cell activity in Arabidopsis (2019)
  • Author(s): Shang Erlei、Ito Toshiro、Sun Bo
  • Journal Title: Plant Signaling & Behavior Volume: 14 Issue: 11 Pages: 1659706-1659706
  • DOI: 10.1080/15592324.2019.1659706
  • Peer Reviewed
• [Journal Article] Abscisic acid‐dependent histone demethylation during postgermination growth arrest in Arabidopsis (2019)
  • Author(s): Wu Jinfeng、Ichihashi Yasunori、Suzuki Takamasa、Shibata Arisa、Shirasu Ken、Yamaguchi Nobutoshi、Ito Toshiro
  • Journal Title: Plant, Cell & Environment Volume: - Issue: 7 Pages: 2198-2214
  • DOI: 10.1111/pce.13547
  • Peer Reviewed / Open Access
• [Journal Article] Integration of transcriptional repression and Polycomb-mediated silencing of WUSCHEL in floral meristems (2019)
  • Author(s): Sun Bo、Zhou Yingying、Cai Jie、Shang Erlei、Yamaguchi Nobutoshi、Xiao Jun、Looi Liang-Sheng、Wee Wan-Yi、Gao Xiuying、Wagner Doris、Ito Toshiro
  • Journal Title: The Plant Cell Volume: - Issue: 7 Pages: 1488-1505
  • DOI: 10.1105/tpc.18.00450
  • NAID: 120006726651
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] When to stop: an update on molecular mechanisms of floral meristem termination (2019)
  • Author(s): Yifeng Xu, Nobutoshi Yamaguchi, Eng-Seng Gan, Toshiro Ito
  • Journal Title: Journal of Experimental Botany Volume: 70 Issue: 6 Pages: 1711-1718
  • DOI: 10.1093/jxb/erz048
  • Peer Reviewed
• [Journal Article] Histone demethylases control root elongation in response to stress-signaling hormone abscisic acid (2019)
  • Author(s): Wu Jinfeng、Yamaguchi Nobutoshi、Ito Toshiro
  • Journal Title: Plant Signaling & Behavior Volume: - Issue: 7 Pages: 1-4
  • DOI: 10.1080/15592324.2019.1604019
  • Peer Reviewed
• [Journal Article] Chromatin-mediated feed-forward auxin biosynthesis in floral meristem determinacy. (2018)
  • Author(s): Yamaguchi, N., Huang, J., Tatsumi, Y., Abe, M., Sugano, S. S., Kojima, M., Takebayashi, Y., Kiba, T., Yokoyama, R., Nishitani, K., Sakakibara, H., and Ito, T.
  • Journal Title: Nature Commun. Volume: 9 Issue: 1 Pages: 5290-5290
  • DOI: 10.1038/s41467-018-07763-0
  • NAID: 120006549567
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Meristem Termination and Organ Number Control in Early Stage of Arabidopsis Flower Development (2018)
  • Author(s): Xu Yifeng、Ito Toshiro
  • Journal Title: Journal of Cell Signaling Volume: 03 Issue: 03
  • DOI: 10.4172/2576-1471.1000186
  • Peer Reviewed
• [Journal Article] In Situ Proximity Ligation Assay to Detect the Interaction Between Plant Transcription Factors and Other Regulatory Proteins (2018)
  • Author(s): Yifeng Xu, Eng-Seng Gan, Toshiro Ito
  • Journal Title: Plant Transcription Factors.Methods in molecular biology (Clifton, N.J.) Volume: 1830 Pages: 325-335
  • DOI: 10.1007/978-1-4939-8657-6_19
  • ISBN: 9781493986569, 9781493986576
  • Peer Reviewed
• [Journal Article] Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis (2018)
  • Author(s): Ze Hong Lee, Nobutoshi Yamaguchi, Toshiro Ito
  • Journal Title: Plant Transcription Factors.Methods in molecular biology (Clifton, N.J.) Volume: 1830 Pages: 93-108
  • DOI: 10.1007/978-1-4939-8657-6_6
  • ISBN: 9781493986569, 9781493986576
  • Peer Reviewed
• [Journal Article] RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells (2018)
  • Author(s): Arai Satoshi、Kriszt Rokus、Harada Kazuki、Looi Liang-Sheng、Matsuda Shogo、Wongso Devina、Suo Satoshi、Ishiura Shoichi、Tseng Yu-Hua、Raghunath Michael、Ito Toshiro、Tsuboi Takashi、Kitaguchi Tetsuya
  • Journal Title: Angewandte Chemie Volume: 130 Issue: 34 Pages: 11039-11044
  • DOI: 10.1002/ange.201804304
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] SUPERMAN regulates floral whorl boundaries through control of auxin biosynthesis. (2018)
  • Author(s): Xu, Y., Prunet, N., Gan, E-S., Wang, Y., Stewart, D., Wellmer, F., Huang, J., Yamaguchi, N., Tatsumi, Y., Kojima, M., Kiba, T., Sakakibara, H., Jack, T.P., Meyerowitz, E.M., Ito, T.
  • Journal Title: EMBO Journal Volume: - Issue: 11
  • DOI: 10.15252/embj.201797499
  • NAID: 120006478564
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Membrane-bound NAC-like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa (2018)
  • Author(s): Guo, S., Dai, S., Singh, P.K., Wang, H., Wang, Y., Tan, L.H., Wee, W., and Ito, T.
  • Journal Title: Frontiers in Plant Science, Plant Biotechnology Volume: 9 Pages: 555-555
  • DOI: 10.3389/fpls.2018.00555
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of floral meristem activity through the interaction of AGAMOUS, SUPERMAN, and CLAVATA3 in Arabidopsis. (2018)
  • Author(s): Uemura, A., Yamaguchi, N, Xu, Y., Wee, W-Y., Ichihashi, Y., Suzuki, T., Shibata, A., Shirasu, K., and Ito, T.
  • Journal Title: Plant Reproduction Volume: 31 Issue: 1 Pages: 89-105
  • DOI: 10.1007/s00497-017-0315-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 花幹細胞の増殖終結機構におけるオーキシン作用の二面性 (2020)
  • Author(s): 伊藤 寿朗
  • Organizer: 第61回日本植物生理学会大阪年会
• [Presentation] Epigenetic regulation in floral meristem determinacy (2019)
  • Author(s): 伊藤 寿朗
  • Organizer: 2019 Collaborative Research Meeting National Institute of Genetics
  • Int'l Joint Research
• [Presentation] Multistep termination of floral stem cell activities (2019)
  • Author(s): 伊藤 寿朗
  • Organizer: ICAR2019(The 30th International Conference on Arabidopsis Research)
  • Int'l Joint Research
• [Presentation] Auxin-mediated termination of floral stem cell activities (2019)
  • Author(s): 伊藤 寿朗
  • Organizer: Principles of pluripotent stem cells underlying plant vitality
  • Int'l Joint Research / Invited
• [Presentation] 花の儚さの分子機構 (2019)
  • Author(s): 伊藤 寿朗
  • Organizer: 2019年度 近畿植物学会講演会
  • Invited
• [Presentation] Multistep termination of floral stem cell activities (2019)
  • Author(s): 伊藤 寿朗
  • Organizer: Japan-Taiwan Plant Biology 2019 (JTPB2019)
  • Int'l Joint Research / Invited
• [Presentation] Spatio-temporal regulation of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: PSDB 10th Annual National Convention
  • Int'l Joint Research / Invited
• [Presentation] Memory and induced forgetting in plants (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: University of the Philippines-Diliman Ateneo de University 大学訪問セミナー
• [Presentation] 植物における記憶定着と能動的忘却システム (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: さきがけ懇親会
• [Presentation] Spatio-temporal regulation of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: Henan University大学訪問セミナー
  • Invited
• [Presentation] Multi-step termination of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: 福建農林大学さくらサイエンスプラン
• [Presentation] Auxin-mediated multistep termination of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: IPMB 2018
  • Int'l Joint Research / Invited
• [Presentation] 花幹細胞の時空間的な運命制御 (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: 京都大学形態統御学分科セミナー
  • Invited
• [Presentation] Auxin-mediated floral meristem determinacy control (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: 25th ICSPR 2018
  • Int'l Joint Research / Invited
• [Presentation] Auxin-mediated multistep termination of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: Meristem 2018
  • Int'l Joint Research / Invited