環境応答により雌雄を産み分けるミジンコの性スペクトラム

• Project Area: Spectrum of the Sex: a continuity of phenotypes between female and male
• Project/Area Number: 18H04884
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Research Institution: Osaka University
• Principal Investigator: 加藤 泰彦 大阪大学, 工学研究科, 准教授 (60415932)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000); Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: オオミジンコ / 性スペクトラム / ダブルセックス遺伝子 / 長鎖ノンコーディングＲＮＡ / エピゲノム / 共生菌

Outline of Annual Research Achievements

前年度に行ったオス化の程度が異なるオス決定因子Dsx1変異体を用いたRNA-seqデータから、Dsx1と相関のある449個の遺伝子を見出した。これらの遺伝子の発現は Dsx1の変異に依存して変化しており、Dsx1の下流因子であることが示唆された。興味深いことに、これらの遺伝子の大半（398個）は Dsx1 発現と負の相関を示しており、Dsx1によって直接的または間接的に抑制されていることを示唆された。RNA-Seq解析において見いだされたメスで高発現する遺伝子群には「輸送」と「代謝」に関わる遺伝子が多く、これらの中にはアミノ酸や糖の生合成遺伝子が多く含まれていた。一方で、オスで高発現する遺伝子群には、「シグナル伝達経路」に関係する遺伝子が多かった。この結果は、Dsx1が転写因子として複数のシグナル伝達経路を活性化して器官形成をオス型に向かわせる一方で、抑制因子として代謝を低下させていることを示唆している。以上の解析から、性スペトラム上の位置を変動させるDsx1の下流で働く因子をリストアップすることができた。
一方で、 Dsx1 を活性化する長鎖ノンコーディングＲＮＡの性スペトラム上の位置決定における役割を明らかにするために、長鎖ノンコーディングＲＮＡに結合するタンパク質を探索も行った。長鎖ノンコーディングＲＮＡのコア配列に結合するタンパク質をトラップして質量分析を行い、２つの結合タンパク質を見出すことに成功した。

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Mutation of the Cytochrome P450 CYP360A8 Gene Increases Sensitivity to Paraquat in Daphnia magna (2021)
  • Author(s): Religia Pijar、Nguyen Nhan Duc、Nong Quang Dang、Matsuura Tomoaki、Kato Yasuhiko、Watanabe Hajime
  • Journal Title: Environmental Toxicology and Chemistry Volume: 0 Issue: 5 Pages: 0-0
  • DOI: 10.1002/etc.4970
  • Peer Reviewed / Open Access
• [Journal Article] Complete mitochondrial genome of the freshwater water flea Daphnia magna NIES strain (Cladocera, Daphniidae): Rearrangement of two ribosomal RNA genes (2020)
  • Author(s): Lee Jin-Sol、Kim Duck-Hyun、Choi Beom-Soon、Kato Yasuhiko、Watanabe Hajime、Lee Jae-Seong
  • Journal Title: Mitochondrial DNA Part B Volume: 5 Issue: 2 Pages: 1822-1823
  • DOI: 10.1080/23802359.2020.1750995
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Oligosaccharides derived from dragon fruit modulate gut microbiota, reduce oxidative stress and stimulate toll-pathway related gene expression in freshwater crustacean Daphnia magna (2020)
  • Author(s): Sangkuanun Thanwarat、Wichienchot Santad、Kato Yasuhiko、Watanabe Hajime、Peerakietkhajorn Saranya
  • Journal Title: Fish & Shellfish Immunology Volume: 103 Pages: 126-134
  • DOI: 10.1016/j.fsi.2020.04.046
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Two Doublesex1 mutants revealed a tunable gene network underlying intersexuality in Daphnia magna (2020)
  • Author(s): Nong Quang Dang、Matsuura Tomoaki、Kato Yasuhiko、Watanabe Hajime
  • Journal Title: PLOS ONE Volume: 15 Issue: 8 Pages: e0238256-e0238256
  • DOI: 10.1371/journal.pone.0238256
  • Peer Reviewed / Open Access
• [Journal Article] Roles of and cross-talk between ecdysteroid and sesquiterpenoid pathways in embryogenesis of branchiopod crustacean Daphnia magna (2020)
  • Author(s): Adhitama Nikko、Kato Yasuhiko、Matsuura Tomoaki、Watanabe Hajime
  • Journal Title: PLOS ONE Volume: 15 Issue: 10 Pages: e0239893-e0239893
  • DOI: 10.1371/journal.pone.0239893
  • Peer Reviewed / Open Access
• [Journal Article] Caloric restriction upregulates the expression ofDNMT3.1, lacking the conserved catalytic domain, inDaphnia magna (2020)
  • Author(s): Nguyen Nhan Duc、Matsuura Tomoaki、Kato Yasuhiko、Watanabe Hajime
  • Journal Title: genesis Volume: 58 Issue: 12
  • DOI: 10.1002/dvg.23396
  • Peer Reviewed
• [Journal Article] Production of genome-edited Daphnia for heavy metal detection by fluorescence (2020)
  • Author(s): Arao Takuto、Kato Yasuhiko、Nong Quang Dang、Yamamoto Hiroshi、Watanabe Haruna、Matsuura Tomoaki、Tatarazako Norihisa、Tani Kazune、Okamoto Akira、Matsumoto Takeru、Watanabe Hajime
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 21490-21490
  • DOI: 10.1038/s41598-020-78572-z
  • Peer Reviewed / Open Access
• [Journal Article] Reduction of histamine and enhanced spinning behavior of Daphnia magna caused by scarlet mutant (2020)
  • Author(s): Ismail Nur Izzatur Binti、Kato Yasuhiko、Matsuura Tomoaki、G?mez‐Canela Cristian、Barata Carlos、Watanabe Hajime
  • Journal Title: genesis Volume: 59 Issue: 3
  • DOI: 10.1002/dvg.23403
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Variations in effects of ectosymbiotic microbes on the growth rates among different species and genotypes of Daphnia fed different algal diets (2020)
  • Author(s): Zhang Xuan、Ohtsuki Hajime、Makino Wataru、Kato Yasuhiko、Watanabe Hajime、Urabe Jotaro
  • Journal Title: Ecological Research Volume: 36 Issue: 2 Pages: 303-312
  • DOI: 10.1111/1440-1703.12194
  • Peer Reviewed / Open Access
• [Journal Article] Atrazine exposed phytoplankton causes the production of non-viable offspring on Daphnia magna (2019)
  • Author(s): Religia Pijar、Kato Yasuhiko、Fukushima Ery Odette、Matsuura Tomoaki、Muranaka Toshiya、Watanabe Hajime
  • Journal Title: Marine Environmental Research Volume: 145 Pages: 177-183
  • DOI: 10.1016/j.marenvres.2019.02.007
  • Peer Reviewed
• [Journal Article] A 5′ UTR-Overlapping LncRNA Activates the Male-Determining Gene doublesex1 in the Crustacean Daphnia magna (2018)
  • Author(s): Kato Yasuhiko、Perez Christelle Alexa G.、Mohamad Ishak Nur Syafiqah、Nong Quang D.、Sudo Yuumi、Matsuura Tomoaki、Wada Tadashi、Watanabe Hajime
  • Journal Title: Current Biology Volume: 28 Issue: 11 Pages: 1811-1817
  • DOI: 10.1016/j.cub.2018.04.029
  • Peer Reviewed / Open Access
• [Journal Article] Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna (2018)
  • Author(s): Toerner Kerstin、Nakanishi Tsuyoshi、Matsuura Tomoaki、Kato Yasuhiko、Watanabe Hajime
  • Journal Title: PLOS ONE Volume: 13 Issue: 6 Pages: e0198023-e0198023
  • DOI: 10.1371/journal.pone.0198023
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Monitoring ecdysteroid activities using genetically encoded reporter gene in Daphnia magna (2018)
  • Author(s): Adhitama Nikko、Matsuura Tomoaki、Kato Yasuhiko、Watanabe Hajime
  • Journal Title: Marine Environmental Research Volume: 140 Pages: 375-381
  • DOI: 10.1016/j.marenvres.2018.07.003
  • Peer Reviewed
• [Journal Article] Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer (2018)
  • Author(s): Elfaramawy Maie A.、Fujii Satoshi、Uyeda Atsuko、Osaki Toshihisa、Takeuchi Shoji、Kato Yasuhiko、Watanabe Hajime、Matsuura Tomoaki
  • Journal Title: Chemical Communications Volume: 54 Issue: 86 Pages: 12226-12229
  • DOI: 10.1039/c8cc06804f
  • Peer Reviewed
• [Journal Article] Generation of white-eyed Daphnia magna mutants lacking scarlet function (2018)
  • Author(s): Ismail Nur Izzatur Binti、Kato Yasuhiko、Matsuura Tomoaki、Watanabe Hajime
  • Journal Title: PLOS ONE Volume: 13 Issue: 11 Pages: e0205609-e0205609
  • DOI: 10.1371/journal.pone.0205609
  • Peer Reviewed / Open Access
• [Presentation] ミジンコの性 決定を制御する long noncoding RNA (2020)
  • Author(s): 加藤泰彦
  • Organizer: 日本遺伝学会第 92 回大会
  • Invited
• [Presentation] A 5´ UTR-overlapping LncRNA Activates the Male-determining Gene doublesex1 in the Crustacean Daphnia magna (2018)
  • Author(s): Yasuhiko Kato, Christelle Alexa G. Pelez, Nur Syafiqah Mohamad Ishak, Quang D. Nong, Tomoaki Matsuura, Tadashi Wada, Hajime Watanabe
  • Organizer: 第 20 回 日本 RNA 学会年会
• [Presentation] Monitoring of chemical and metal contaminants using engineered Daphnia magna (2018)
  • Author(s): Yasuhiko Kato, Hajime Watanabe
  • Organizer: Second Interdisciplinary and Research Alumni Symposium iJaDe2018
  • Int'l Joint Research
• [Presentation] オオミジンコにおける内分泌かく乱物質検出のためのGAL4/UASシステムの確立 (2018)
  • Author(s): 加藤泰彦、山崎勇人、松浦友亮、渡邉肇
  • Organizer: 第70回日本生物工学会大会
• [Presentation] Visualization of ecdysteroid activities using genetically encoded reporter gene in Daphnia magna (2018)
  • Author(s): Nikko Adhitama, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
  • Organizer: 第70回日本生物工学会大会
• [Presentation] オオミジンコの遺伝子操作法の開発と応用 (2018)
  • Author(s): 加藤泰彦、志賀靖弘、時下進一、山形秀夫、渡邉肇
  • Organizer: 東京薬科大学生命科学部25周年記念シンポジウム
• [Presentation] 環境応答により雌雄を産み分けるミジンコの性スペクトラム (2018)
  • Author(s): Yasuhiko Kato、Christelle Alexa G. Perez、Quang D. Nong、Hajime Watanabe
  • Organizer: 新学術領域研究 性スペクトラム 第二回 領域班会議
• [Presentation] A 5 ' UTR-Overlapping LncRNA Activates the Male- Determining Gene doublesex1 in the Crustacean Daphnia magna (2018)
  • Author(s): Yasuhiko Kato、Christelle Alexa G. Perez、Quang D. Nong、Hajime Watanabe
  • Organizer: JAJ RNA 2018
  • Int'l Joint Research / Invited
• [Presentation] オオミジンコにおけるHSP70プロモーターを利用した熱誘導型遺伝子発現系の開発 (2018)
  • Author(s): 加藤泰彦、清水里奈、渡邉肇
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] 環境応答により雌雄を産み分けるミジンコの性スペクトラム (2018)
  • Author(s): 加藤泰彦、Quang Dang Nong、渡邉肇
  • Organizer: 「先進ゲノム支援」2018年度拡大班会議
• [Presentation] Alterations in the activity of the sex determination factor dsx1 resulted in different intersex phenotypes in Daphnia magna (2018)
  • Author(s): Quang Dang Nong , Yasuhiko Kato , Hajime Watanabe
  • Organizer: 「先進ゲノム支援」2018年度拡大班会議
• [Presentation] Possibility of chemical communication among Daphnia individuals for reproductive strategy (2018)
  • Author(s): Yasuhiko Kato, Hajime Watanabe
  • Organizer: Frontier Research on Chemical Communications (ISCC2019)
  • Int'l Joint Research