マルチモーダル入力を適応的行動出力に収斂する脳情報動態の解明

• Project Area: Brain information dynamics underlying multi-area interconnectivity and parallel processing
• Project/Area Number: 18H05113
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Research Institution: The University of Tokyo
• Principal Investigator: 能瀬 聡直 東京大学, 大学院新領域創成科学研究科, 教授 (30260037)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ショウジョウバエ / コネクトミクス / マルチモーダル感覚統合 / カルシウムイメージング / 脳情報動態

Outline of Annual Research Achievements

動物は時々刻々と変化する外界の状況を複数の感覚モダリティを通じて解釈することで適切な行動を決定し実行する。複数のモ ダリティにまたがった判断、行動選択がなされるには、異なった脳内経路を経由して処理される各モダリティの情報が脳内のどこかで統合される必要がある。本研究では脳全体を視野に入れた神経活動測定が可能で、かつコネクトミクス解析により回路構造を網羅的に同定できるショウジョウバエ幼虫をモデルとして用い、マルチモーダル感覚統合の脳情報動態を理解することを目的とした。
幼虫は頭部に侵害刺激を受けると後退運動により逃避する。この逃避行動は、２つのモダリティ、視覚（頭部への青色光の照射 ）および触覚（頭部への機械的刺激）によって誘起される。申請者らは以前の研究において視覚刺激、触覚刺激が後退運動を誘起する過程を制御するコマンドニューロンをそれぞれ同定していた（Takagi et al., 2017、大浦ら、未発表）。また昨年度計画において視覚刺激、触覚刺激から後退運動出力に至る神経経路をコネクトミクス解析により探索し、マルチモーダル入力の統合に関わるような神経経路の一部を明らかにしていた。本年度はコネクトミクス解析をさらに進め、統合に関わるような新たな経路を見出すとともに、その回路が後退を誘発するだけでなく前進を抑制する可能性を見出した。

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] University of Cambridge(英国)
• [Int'l Joint Research] University of Washington(米国)
• [Int'l Joint Research] University of St Andrews(英国)
• [Int'l Joint Research] University of Freiburg(ドイツ)
• [Int'l Joint Research] Janelia Farm Research Campus(米国)
• [Int'l Joint Research] University of Washington(米国)
• [Int'l Joint Research] University of St Andrews(英国)
• [Journal Article] System level analysis of motor-related neural activities in larval Drosophila (2019)
  • Author(s): Yoon Youngteak、Park Jeonghyuk、Taniguchi Atsushi、Kohsaka Hiroshi、Nakae Ken、Nonaka Shigenori、Ishii Shin、Nose Akinao
  • Journal Title: Journal of Neurogenetics Volume: 33 Issue: 3 Pages: 179-189
  • DOI: 10.1080/01677063.2019.1605365
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regulation of forward and backward locomotion through intersegmental feedback circuits in Drosophila larvae (2019)
  • Author(s): Kohsaka Hiroshi、Zwart Maarten F.、Fushiki Akira、Fetter Richard D.、Truman James W.、Cardona Albert、Nose Akinao
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 1-11
  • DOI: 10.1038/s41467-019-10695-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Data-driven analysis of motor activity implicates 5-HT2A neurons in backward locomotion of larval Drosophila (2018)
  • Author(s): Park Jeonghyuk、Kondo Shu、Tanimoto Hiromu、Kohsaka Hiroshi、Nose Akinao
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 1-15
  • DOI: 10.1038/s41598-018-28680-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Functional connectomics of motor circuits in Drosophila larvae (2019)
  • Author(s): Nose A
  • Organizer: Max Planck / HHMI Connectomics Conference Berlin 2019
  • Int'l Joint Research / Invited
• [Presentation] Experience-driven development of motor circuits in Drosophila (2019)
  • Author(s): Zeng X, Kawasaki T, Inada K, Kazama H, Nose A
  • Organizer: 52th Annual Meeting of the Japanese Society of Developmental Biologists
  • Int'l Joint Research / Invited
• [Presentation] Embryonic development of the motor circuits in Drosophila: emergence of coordinated neural activities and the role of sensory feedback (2019)
  • Author(s): Zeng X, Kawasaki T, Inada K, Kazama H, Nose A
  • Organizer: 第42回日本神経科学大会
  • Int'l Joint Research / Invited
• [Presentation] Regulation of motor circuit development by proprioceptive feedback (2019)
  • Author(s): A. Nose
  • Organizer: 2019 ASIA-PACIFIC DRORSOPHILA NEUROBIOLOGY CONFERENCE
  • Int'l Joint Research / Invited
• [Presentation] Regulation of embryonic motor circuit formation by somatosensory feedback (2018)
  • Author(s): A. Nose, X. Zeng, K. Inada and H. Kazama
  • Organizer: The Joint Research Area Meeting Scientific Research on Innovative Areas. “Artificial Intelligence and Brain Science” and “Adaptive Circuit Shift”
  • Int'l Joint Research
• [Presentation] Formation of the neuronal connectivity that regulates divergent action selections in Drosophila larvae (2018)
  • Author(s): S. Takagi and A. Nose
  • Organizer: SfN－Society for Neuroscience 48th Annual Meeting
  • Int'l Joint Research
• [Presentation] Optogenetic and connectome analyses of the neural circuits regulating escape behaviors in Drosophila larvae (2018)
  • Author(s): S. Takagi and A. Nose
  • Organizer: 第10回光操作研究会・第2回脳情報動態 合同国際シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] Embryonic development of the motor circuits in Drosophila: emergence of coordinated neural activities and the role of sensory feedback (2018)
  • Author(s): X. Zeng, T. Kawasaki, K. Inada, H. Kazama and A. Nose
  • Organizer: Behavioral Neurogenetics of Drosophila Larva
  • Int'l Joint Research / Invited
• [Presentation] Neural circuits regulating divergent escape responses in Drosophila larvae: implication for evo-devo of adaptive behaviors (2018)
  • Author(s): S. Takagi and A. Nose
  • Organizer: The 41st Annual Meeting of the Molecular Biology Society of Japan
  • Int'l Joint Research / Invited
• [Book] Neuroscience (2019)
  • Author(s): S. Takagi and A. Nose
  • Total Pages: 6
  • Publisher: Circuit architecture for somatotopic action selection in Invertebrates
• [Remarks] 能瀬・高坂研究室
  • URL: http://bio.phys.s.u-tokyo.ac.jp/
• [Remarks] 能瀬・高坂研究室
  • URL: http://bio.phys.s.u-tokyo.ac.jp/index.html