高等哺乳動物における脳情報動態の基盤構築形成機構の解明

• Project Area: Brain information dynamics underlying multi-area interconnectivity and parallel processing
• Project/Area Number: 18H05119
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Research Institution: Kanazawa University
• Principal Investigator: 河崎 洋志 金沢大学, 医学系, 教授 (50303904)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 大脳皮質

Outline of Annual Research Achievements

大脳皮質は脳情報処理の中枢であり、ヒトに至る進化の過程で著しく発達してきた。従って、脳情報処理の基盤となる大脳皮質の神経回路の進化のプロセス、高等哺乳動物における大脳皮質神経回路の解析、およびその形成過程の解明は重要である。我々は、発達した大脳皮質を持つ食肉類哺乳動物フェレットを用いて、この問題にアプローチしてきた。ヒトやサルなどの形成過程の大脳皮質には、外側線維層(outer fiber layer)および内側線維層(inner fiber layer)という2つの線維層が存在することが知られていたが、マウスにはこの区分は見いだされておらず、投射先およびその形成過程については不明な点が多かった。我々はこれまでに、子宮内エレクトロポレーション法をフェレットに応用することに成功し、フェレットの大脳皮質での遺伝子発現を可能としてきた。そこで本研究では、フェレット大脳皮質神経細胞へGFPを導入し神経回路を可視化することにより、フェレット大脳皮質の線維層の解析を行った。その結果、ヒトやサルと同様にフェレットにも外側線維層と内側線維層が存在していることを見いだした。投射先を解析した結果、外側線維層は近隣の大脳皮質へ、内側線維層は対側大脳皮質もしくは皮質下へ投射していることが分かった。さらにマウスとフェレットとの比較解析により、これまで報告されていなかったマウスにも外側線維層および内側線維層に対応する線維層が存在すること、また進化の過程で外側線維層の量が著しく増加していることを見いだした。これらの研究の成果は、大脳皮質における脳情報動態や大脳皮質の進化プロセスの解明に繋がる。

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Int'l Joint Research] INMED, Aix-Marseille University, INSERM(フランス)
• [Journal Article] Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation (2021)
  • Author(s): Hamabe-Horiike Toshihide、Kawasaki Kanji、Sakashita Masataka、Ishizu Chihiro、Yoshizaki Tomokazu、Harada Shin-ichi、Ogawa-Ochiai Keiko、Shinmyo Yohei、Kawasaki Hiroshi
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 4864-4864
  • DOI: 10.1038/s41598-021-84210-z
  • Peer Reviewed / Open Access
• [Journal Article] Phosphorylation of GAP-43 T172 is a molecular marker of growing axons in a wide range of mammals including primates (2021)
  • Author(s): Okada Masayasu、Kawagoe Yosuke、Sato Yuta、Nozumi Motohiro、Ishikawa Yuya、Tamada Atsushi、Yamazaki Hiroyuki、Sekino Yuko、Kanemura Yonehiro、Shinmyo Yohei、Kawasaki Hiroshi、Kaneko Naoko、Sawamoto Kazunobu、Fujii Yukihiko、Igarashi Michihiro
  • Journal Title: Molecular Brain Volume: 14 Issue: 1 Pages: 66-66
  • DOI: 10.1186/s13041-021-00755-0
  • Peer Reviewed / Open Access
• [Journal Article] The expression of aristaless-related homeobox in neural progenitors of gyrencephalic carnivore ferrets (2021)
  • Author(s): Maeyama Hiroki、Shinmyo Yohei、Kawasaki Hiroshi
  • Journal Title: Biochemistry and Biophysics Reports Volume: 26 Pages: 100970-100970
  • DOI: 10.1016/j.bbrep.2021.100970
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] BMP signaling alters aquaporin-4 expression in the mouse cerebral cortex (2021)
  • Author(s): Morita K., Matsumoto N., Saito K., Hamabe-Horiike T., Mizuguchi K., Shinmyo Y. and Kawasaki H.
  • Journal Title: Scientific Reports
  • Peer Reviewed / Open Access
• [Journal Article] Visualization of the Retina in Intact Eyes of Mice and Ferrets Using a Tissue Clearing Method (2020)
  • Author(s): Ye Yunyan、Dinh Duong Tung Anh、Saito Kengo、Shinmyo Yohei、Ichikawa Yoshie、Higashide Tomomi、Kagami Kyosuke、Fujiwara Hiroshi、Sugiyama Kazuhisa、Kawasaki Hiroshi
  • Journal Title: Translational Vision Science & Technology Volume: 9 Issue: 3 Pages: 1-1
  • DOI: 10.1167/tvst.9.3.1
  • Peer Reviewed / Open Access
• [Journal Article] Structural Changes and Astrocyte Response of the Lateral Geniculate Nucleus in a Ferret Model of Ocular Hypertension (2020)
  • Author(s): Fujishiro Takashi、Honjo Megumi、Kawasaki Hiroshi、Asaoka Ryo、Yamagishi Reiko、Aihara Makoto
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Issue: 4 Pages: 1339-1339
  • DOI: 10.3390/ijms21041339
  • Peer Reviewed / Open Access
• [Journal Article] In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation (2020)
  • Author(s): Kalebic Nereo、Langen Barbara、Helppi Jussi、Kawasaki Hiroshi、Huttner Wieland B.
  • Journal Title: Journal of Visualized Experiments Volume: 159 Issue: 159
  • DOI: 10.3791/61171
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The origin and development of subcortical U-fibers in gyrencephalic ferrets. (2020)
  • Author(s): Yoshino M, Saito K, Kawasaki K, Horiike T, Shinmyo Y, Kawasaki H.
  • Journal Title: Mol Brain Volume: 13 Issue: 1 Pages: 37-37
  • DOI: 10.1186/s13041-020-00575-8
  • Peer Reviewed / Open Access
• [Journal Article] A discrete subtype of neural progenitor crucial for cortical folding in the gyrencephalic mammalian brain (2020)
  • Author(s): Matsumoto Naoyuki、Tanaka Satoshi、Horiike Toshihide、Shinmyo Yohei、Kawasaki Hiroshi
  • Journal Title: eLife Volume: 9
  • DOI: 10.7554/elife.54873
  • Peer Reviewed / Open Access
• [Journal Article] A novel third mesh-like myometrial layer connects the longitudinal and circular muscle fibers -A potential stratum to coordinate uterine contractions- (2020)
  • Author(s): Kagami Kyosuke, Ono Masanori, Iizuka Takashi, Matsumoto Takeo, Hosono Takashi, Sekizuka-Kagami Naomi, Shinmyo Yohei, Kawasaki Hiroshi, Fujiwara Hiroshi
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 8274-8274
  • DOI: 10.1038/s41598-020-65299-0
  • Peer Reviewed / Open Access
• [Journal Article] Neocortical Expansion Due to Increased Proliferation of Basal Progenitors Is Linked to Changes in Their Morphology (2019)
  • Author(s): Kalebic Nereo、Gilardi Carlotta、Stepien Barbara、Wilsch-Br?uninger Michaela、Long Katherine R.、Namba Takashi、Florio Marta、Langen Barbara、Lombardot Benoit、Shevchenko Anna、Kilimann Manfred W.、Kawasaki Hiroshi、Wimberger Pauline、Huttner Wieland B.
  • Journal Title: Cell Stem Cell Volume: 24 Issue: 4 Pages: 535-550.e9
  • DOI: 10.1016/j.stem.2019.02.017
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] YAP Activity Is Necessary and Sufficient for Basal Progenitor Abundance and Proliferation in the Developing Neocortex (2019)
  • Author(s): Kostic Milos、Paridaen Judith T.M.L.、Long Katherine R.、Kalebic Nereo、Langen Barbara、Gr?bling Nannette、Wimberger Pauline、Kawasaki Hiroshi、Namba Takashi、Huttner Wieland B.
  • Journal Title: Cell Reports Volume: 27 Issue: 4 Pages: 1103-1118.e6
  • DOI: 10.1016/j.celrep.2019.03.091
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] FGF Signaling Directs the Cell Fate Switch from Neurons to Astrocytes in the Developing Mouse Cerebral Cortex. (2019)
  • Author(s): Dinh Duong TA, Hoshiba Y, Saito K, Kawasaki K, Ichikawa Y, Matsumoto N, Shinmyo Y, Kawasaki H.
  • Journal Title: J Neurosci Volume: 39 Issue: 31 Pages: 6081-6094
  • DOI: 10.1523/jneurosci.2195-18.2019
  • Peer Reviewed
• [Journal Article] Characterization of the Inner and Outer Fiber Layers in the Developing Cerebral Cortex of Gyrencephalic Ferrets. (2019)
  • Author(s): Saito K, Mizuguchi K, Horiike T, Dinh Duong TA, Shinmyo Y, Kawasaki H.
  • Journal Title: Cereb Cortex. Volume: 29 Issue: 10 Pages: 4303-4311
  • DOI: 10.1093/cercor/bhy312
  • Peer Reviewed
• [Journal Article] Homeobox code model of heterodont tooth in mammals revised (2019)
  • Author(s): Wakamatsu Yoshio、Egawa Shiro、Terashita Yukari、Kawasaki Hiroshi、Tamura Koji、Suzuki Kunihiro
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 12865-12865
  • DOI: 10.1038/s41598-019-49116-x
  • Peer Reviewed / Open Access
• [Journal Article] YAP activity is necessary and sufficient for basal progenitor abundance and proliferation in the developing neocortex (2019)
  • Author(s): Kostic M., Paridaen J.T.M.L., Long K., Kalebic N., Langen B., Grubling N., Wimberger P., Kawasaki H., Namba T. and Huttner W.B.
  • Journal Title: Cell Reports
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular Investigations of the Development and Diseases of Cerebral Cortex Folding using Gyrencephalic Mammal Ferrets (2018)
  • Author(s): Kawasaki Hiroshi
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 41 Issue: 9 Pages: 1324-1329
  • DOI: 10.1248/bpb.b18-00142
  • NAID: 130007479897
  • ISSN: 0918-6158, 1347-5215
  • Year and Date: 2018-09-01
  • Peer Reviewed
• [Journal Article] Pathophysiological analyses of leptomeningeal heterotopia using gyrencephalic mammals (2018)
  • Author(s): Matsumoto Naoyuki、Kobayashi Naoki、Uda Natsu、Hirota Miwako、Kawasaki Hiroshi
  • Journal Title: Human Molecular Genetics Volume: 27 Issue: 6 Pages: 985-991
  • DOI: 10.1093/hmg/ddy014
  • Peer Reviewed
• [Journal Article] Correct laminar positioning in the neocortex influences proper dendritic and synaptic development (2018)
  • Author(s): Martineau F. S., Sahu S., Plantier V., Buhler E., Schaller F., Fournier L., Chazal G., Kawasaki H., Represa A., Watrin F. and Manent J.-B.
  • Journal Title: Cerebral Cortex Volume: 28 Issue: 8 Pages: 2976-2990
  • DOI: 10.1093/cercor/bhy113
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Distribution and morphological features of microglia in the developing cerebral cortex of gyrencephalic mammals (2018)
  • Author(s): Mizuguchi K., Horiike T., Matsumoto N., Ichikawa Y., Shinmyo Y. and Kawasaki H.
  • Journal Title: Neurochemical Research Volume: 43 Issue: 5 Pages: 1075-1085
  • DOI: 10.1007/s11064-018-2520-0
  • Description: 掲載確定 印刷中
  • Peer Reviewed
• [Journal Article] Three-dimensional evaluation of murine ovarian follicles using a modified CUBIC tissue clearing method. (2018)
  • Author(s): Kagami K., Shinmyo Y., Ono M., Kawasaki H. and Fujiwara H.
  • Journal Title: Reproductive Biology and Endocrinology Volume: 16 Issue: 1 Pages: 72-72
  • DOI: 10.1186/s12958-018-0381-7
  • Peer Reviewed / Open Access
• [Presentation] 細胞外小胞を介したシヌクレイン伝播機構の解明 (2020)
  • Author(s): 河原 裕憲、奥野 龍禎、八杉 徹雄、佐藤 純、新明 洋平、河崎 洋志、望月 秀樹、華山 力成
  • Organizer: 第43回 日本神経科学大会
• [Presentation] フェレット大脳皮質の外側放射状グリア増加と脳回形成におけるShhシグナルの役割 (2020)
  • Author(s): 松本 直之, 田中 智, 堀池 俊秀, 新明 洋平, 河崎 洋志
  • Organizer: 第43回 日本神経科学大会
• [Presentation] フェレット大脳におけるU-fiberの形成過程解析 (2020)
  • Author(s): 吉野 真優子, 齋藤 健吾, 川崎 寛二, 堀池 俊秀, 新明 洋平, 河崎 洋志
  • Organizer: 第43回 日本神経科学大会
• [Presentation] フェレットを用いた大脳皮質の形成と進化、異常病態の解析 (2020)
  • Author(s): 河崎洋志
  • Organizer: 第50回日本神経精神薬理学会年会・42回日本生物学的精神医学会年会・第4回日本精神薬学会総会・学術集会
  • Invited
• [Presentation] マウスとフェレットを用いた大脳皮質の形成と進化のメカニズム解析 (2020)
  • Author(s): 河崎洋志
  • Organizer: 第63回日本神経化学会大会
  • Invited
• [Presentation] フェレットとマウスを用いた大脳皮質の形成、進化と異常病態の解析 (2020)
  • Author(s): 河崎洋志
  • Organizer: 第61回日本神経病理学会総会学術研究会
  • Invited
• [Presentation] 子宮内エレクトロポレーション法を用いた大脳皮質グリア細胞選択的な遺伝子操作 (2020)
  • Author(s): 堀池 俊秀、川崎 寛二、坂下正考、石津 千裕、新明 洋平、原田 真市、小川 恵子、河崎 洋志
  • Organizer: 第43回日本分子生物学会年会
• [Presentation] Mechanisms underlying the formation and evolution of cortical folds (2020)
  • Author(s): Kawasaki H.
  • Organizer: 遺伝研研究会「哺乳類脳の機能的神経回路の構築メカニズム」
  • Invited
• [Presentation] Uncovering the mechanisms underlying the formation of the cerebral cortex using gyrencephalic ferrets (2019)
  • Author(s): Kawasaki H
  • Organizer: 19th HFSP Awardees Meeting
  • Int'l Joint Research
• [Presentation] フェレットとマウスにおける形成期大脳皮質の神経線維層の解析 (2019)
  • Author(s): 齋藤健吾、水口敬司、堀池俊秀、Dinh Duong Tung Anh、新明洋平、河崎洋志
  • Organizer: 第42回 日本神経科学大会
• [Presentation] Investigation of the mechanisms underlying gyrification of the cerebral cortex using gyrencephalic ferrets (2019)
  • Author(s): Hiroshi Kawasaki, Yohei Shinmyo, Naoyuki Matsumoto
  • Organizer: Annual Meeting for the Society of Neuroscience 2019
  • Int'l Joint Research
• [Presentation] Characterization of fiber layers in the developing cerebral cortex of ferrets and mice (2019)
  • Author(s): K. SAITO, K. MIZUGUCHI, T. HORIIKE, T. A. DINH DUONG, Y. SHINMYO, H. KAWASAKI
  • Organizer: Annual Meeting for the Society of Neuroscience 2019
  • Int'l Joint Research
• [Presentation] フェレット大脳におけるU-fiberの形成過程解析 (2019)
  • Author(s): 吉野 真優子、 齋藤 健吾、川崎 寛二、堀池 俊秀、新明 洋平、河崎 洋志
  • Organizer: 次世代脳プロジェクト 冬のシンポジウム2019
• [Presentation] Investigation of fiber layers in the developing cerebral cortex of ferrets and mice (2019)
  • Author(s): Kengo Saito, Keishi Mizuguchi, Toshihide Horiike, Tung Anh Dinh Duong, Yohei Shinmyo and Hiroshi Kawasaki
  • Organizer: 第12回神経発生討論会
  • Int'l Joint Research
• [Presentation] FGF signaling directs the cell fate switch from neurons to astrocytes in the developing mouse cerebral cortex (2019)
  • Author(s): Tung Anh Dinh Duong, Yoshio Hoshiba, Kengo Saito, Kanji Kawasaki, Yoshie Ichikawa, Naoyuki Matsumoto, Yohei Shinmyo and Hiroshi Kawasaki
  • Organizer: 第12回神経発生討論会
  • Int'l Joint Research
• [Presentation] フェレットとマウスを用いた大脳皮質形成機構とその異常疾患病態の解析 (2019)
  • Author(s): 河崎洋志
  • Organizer: 第18回日本再生医療学会総会
  • Invited
• [Presentation] Folding of the cerebral cortex requires Cdk5 in upper-layer neurons in gyrencephalic mammals (2018)
  • Author(s): Y. Shinmyo, Y. Terashita, T.A. Dinh Duong, T. Horiike, M. Kawasumi, K. Hosomichi, A. Tajima, H. Kawasaki,
  • Organizer: 22nd Biennial Meeting of the International Society for Developmental Neuroscience
  • Int'l Joint Research
• [Presentation] "Gyrification of the cerebral cortex requires FGF signaling in the mammalian brain" (2018)
  • Author(s): N. Matsumoto, Y. Shinmyo, Y. Ichikawa, H. Kawasaki
  • Organizer: 22nd Biennial Meeting of the International Society for Developmental Neuroscience
  • Int'l Joint Research
• [Presentation] Folding of the cerebral cortex requires Cdk5 in upper-layer neurons in gyrencephalic mammals (2018)
  • Author(s): Shinmyo Y, Terashita Y, Dinh Duong TA, Horiike T, Kawasumi M, Hosomichi K, Tajima A, Kawasaki H
  • Organizer: 2018 Korean-Japan Joint Symposium on Neurodevelopment
  • Int'l Joint Research
• [Presentation] Uncovering the mechanisms underlying cortical folding using gyrencephalic ferrets (2018)
  • Author(s): Kawasaki H
  • Organizer: Cortical Evolution 2018
  • Int'l Joint Research
• [Presentation] Uncovering the mechanisms of cortical folding using gyrencephalic mammals (2018)
  • Author(s): Kawasaki H
  • Organizer: Joint symposium of 10th Optogenetics Research Conference and Second International Symposium on Brain Information Dynamics 2018
  • Int'l Joint Research
• [Presentation] マウスとフェレットを用いた大脳皮質形成機構の解析 (2018)
  • Author(s): 河崎洋志
  • Organizer: イメージング数理研究会
  • Invited
• [Presentation] Folding of the cerebral cortex requires Cdk5 in upper-layer neurons in gyrencephalic mammals (2018)
  • Author(s): Yohei Shinmyo, Yukari Terashita, Tung Anh Dinh Duong, Toshihide Horiike, Muneo Kawasumi,Kazuyoshi Hosomichi, Atsushi Tajima, Hiroshi Kawasaki
  • Organizer: 第41回日本神経科学大会
• [Presentation] "Gyrification of the cerebral cortex requires FGF signaling in the mammalian brain" (2018)
  • Author(s): Naoyuki Matsumoto, Yohei Shinmyo, Yoshie Ichikawa, Hiroshi Kawasaki
  • Organizer: 第41回日本神経科学大会
• [Presentation] Molecular mechanisms underlying the formation of the cerebral cortex in the mammalian brain (2018)
  • Author(s): Hiroshi Kawasaki
  • Organizer: 沖縄科学技術大学院大学(OIST)ワークショップ
  • Int'l Joint Research / Invited
• [Presentation] 高等哺乳動物フェレットを用いた大脳皮質の形成機構の解析 (2018)
  • Author(s): 河崎洋志
  • Organizer: 第91回日本生化学会
  • Invited
• [Presentation] Uncovering the mechanisms underlying cortical folding using gyrencephalic ferrets (2018)
  • Author(s): Hiroshi Kawasaki
  • Organizer: Cold Spring Harbor Asia Meeting(Latest Advances in Development & Function of Neuronal Circuits)
  • Int'l Joint Research
• [Presentation] フェレットを用いた大脳皮質形成機構の解析 (2018)
  • Author(s): 河崎洋志
  • Organizer: 神経発達・再生研究会（生理学研究所研究会）
  • Invited
• [Presentation] フェレットを用いた高等哺乳動物の大脳皮質形成機構の解析 (2018)
  • Author(s): 河崎洋志
  • Organizer: 研究推進機構総合研究院 脳学際研究部門 第2回公開シンポジウム
  • Invited
• [Presentation] フェレット大脳皮質の形成期における神経線維層の解析 (2018)
  • Author(s): 斎藤健吾,水口敬司, 堀池俊秀,Tung Anh Dinh Doung, 新明洋平, 河崎洋志
  • Organizer: 第41回分子生物学会年会
• [Presentation] フェレットとマウスを用いた大脳皮質の形成機構の解析 (2018)
  • Author(s): 河崎洋志
  • Organizer: シナプス研究会（生理研研究会）
  • Invited
• [Presentation] Characterization of the inner and outer fiber layers in the developing cerebral cortex of gyrencephalic ferrets (2018)
  • Author(s): 斎藤健吾,水口敬司, 堀池俊秀,Tung Anh Dinh Doung, 新明洋平, 河崎洋志
  • Organizer: 国立遺伝学研究所研究会Circuit construction in the mammalian brain
• [Book] 生理学 (2018)
  • Author(s): 河崎洋志（和田 隆志、奈良 信雄編）
  • Total Pages: 169
  • Publisher: 医歯薬出版
  • ISBN: 426322373X
• [Remarks] 研究室ホームページ
  • URL: http://square.umin.ac.jp/top/kawasaki-lab/
• [Remarks] ResearchMap
  • URL: https://researchmap.jp/hiroshikawasaki
• [Remarks] 河崎研究室ホームページ
  • URL: http://square.umin.ac.jp/top/kawasaki-lab/