行動モードの相転移を実現する脳情報動態

• Project Area: Brain information dynamics underlying multi-area interconnectivity and parallel processing
• Project/Area Number: 18H05123
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Nagoya University
• Principal Investigator: 中野 俊詩 名古屋大学, 理学研究科, 助教 (60608529)
• Project Period (FY): 2018-06-29 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 行動制御 / 神経回路 / 線虫 / 温度走性 / 行動モード

Outline of Annual Research Achievements

線虫C. elegans の2つの行動様式、「ローカルサーチ」と「グローバルサーチ」の違いを特徴付ける行動要素として、後退や急激な方向転換などのターン運動の頻度が挙げられる。すなわち、ローカルサーチモードではターン運動の頻度が上昇し、方向転換を繰り返しながら局所的な探索運動を繰り返すのに対し、グローバルサーチモードでは、方向転換の頻度を著しく減少させることで、より広範な範囲を探索する。最終年度においては、これらの後退などの方向転換運動を制御する神経回路を同定することに成功した。具体的には、行動中の多数の線虫個体をトラッキングするMulti worm tracking システムを駆使して、細胞死を誘導するキャスパーぜやminiSOGなどの遺伝学的手法によって、神経回路への摂動をあたえた集団から行動データを取得した。これらの行動データに対してシミュレーションを用いた解析を行い、ここの行動要素に重要な神経細胞を絞り込むことで、それぞれの行動要素の制御に関与する神経回路を明らかにすることに成功した。これらの解析から、後退や急激な方向転換などの単一の行動要素であっても、線虫が置かれた温度環境に応じて、異なる神経回路が行動制御に関与していることを見出した。また、温度環境に応じた行動制御には、感覚ニューロンと介在ニューロン間の情報伝達様式に変容が生じることが重要であることを明らかにした。これらの研究成果を、とりまとめ、論文発表を行った。(Ikeda et al, PNAS, 2020)。

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Pohang University(韓国)
• [Journal Article] Context-dependent operation of neural circuits underlies a navigation behavior inCaenorhabditis elegans (2020)
  • Author(s): Ikeda Muneki、Nakano Shunji、Giles Andrew C.、Xu Linghuan、Costa Wagner Steuer、Gottschalk Alexander、Mori Ikue
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 117 Issue: 11 Pages: 6178-6188
  • DOI: 10.1073/pnas.1918528117
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Caenorhabditis elegans INX‐4/Innexin is required for the fine‐tuning of temperature orientation in thermotaxis behavior (2020)
  • Author(s): Tsukamoto Satomi、Emmei Taishi、Nakano Shunji、Nishio Nana、Sasakura Hiroyuki、Mori Ikue
  • Journal Title: Genes to Cells Volume: 25 Issue: 3 Pages: 154-164
  • DOI: 10.1111/gtc.12745
  • Peer Reviewed / Open Access
• [Journal Article] Presynaptic MAST kinase controls opposing postsynaptic responses to convey stimulus valence in Caenorhabditis elegans (2020)
  • Author(s): Nakano Shunji、Ikeda Muneki、Tsukada Yuki、Fei Xianfeng、Suzuki Takamasa、Niino Yusuke、Ahluwalia Rhea、Sano Ayana、Kondo Rumi、Ihara Kunio、Miyawaki Atsushi、Hashimoto Koichi、Higashiyama Tetsuya、Mori Ikue
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 117 Issue: 3 Pages: 1638-1647
  • DOI: 10.1073/pnas.1909240117
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Presynaptic MAST Kinase Controls Bidirectional Post-SynapticResponses to Convey Stimulus Valence in C. elegans (2019)
  • Author(s): Shunji Nakano, Muneki Ikeda, Yuki Tsukada, Xianfeng Fei, Takamasa Suzuki, Rhea Ahluwalia, Ayana Sano, Rumi Kondo, Kunio Ihara, Koichi Hashimoto, Tetsuya Higashiyama, Ikue Mori
  • Journal Title: bioRxiv Volume: 609479 Pages: 609479-609479
  • DOI: 10.1101/609479
  • Open Access
• [Journal Article] KIN-4/MAST kinase promotes PTEN-mediated longevity of Caenorhabditis elegans via binding through a PDZ domain (2019)
  • Author(s): An Seon Woo A.、Choi Eun-Seok、Hwang Wooseon、Son Heehwa G.、Yang Jae-Seong、Seo Keunhee、Nam Hyun-Jun、Nguyen Nhung T. H.、Kim Eun Ji E.、Suh Bo Kyoung、Kim Youngran、Nakano Shunji、Ryu Youngjae、Man Ha Chang、Mori Ikue、Park Sang Ki、Yoo Joo-Yeon、Kim Sanguk、Lee Seung-Jae V.
  • Journal Title: Aging Cell Volume: e12906 Issue: 3
  • DOI: 10.1111/acel.12906
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Circuit Degeneracy Facilitates Robustness and Flexibility of Navigation Behavior in C. elegans (2018)
  • Author(s): Ikeda Muneki、Nakano Shunji、Giles Andrew C.、Costa Wagner Steuer、Gottschalk Alexander、Mori Ikue
  • Journal Title: bioRxiv Volume: 385468 Pages: 385468-385468
  • DOI: 10.1101/385468
  • Open Access
• [Journal Article] Identification of animal behavioral strategies by inverse reinforcement learning. (2018)
  • Author(s): Yamaguchi S, Honda N*, Ikeda M, Tsukada Y, Nakano S, Mori I and Ishii S
  • Journal Title: PLoS Computational Biology Volume: 14(5) Issue: 5 Pages: e1006122-e1006122
  • DOI: 10.1371/journal.pcbi.1006122
  • NAID: 120006469168
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] SLO potassium channels antagonize premature decision making in C. elegans (2018)
  • Author(s): Aoki I, Tateyama M, Shimomura T, Ihara K, Kubo Y, Nakano S, Mori I.
  • Journal Title: Commun Biol. Volume: 1 Issue: 1 Pages: 123-123
  • DOI: 10.1038/s42003-018-0124-5
  • Peer Reviewed / Open Access
• [Presentation] Stomatin-dependent control of presynaptic release during a memory-guided navigation behavior in C. elegans (2019)
  • Author(s): Shunji Nakano, Ikue Mori
  • Organizer: The 42nd Annual meeting of the Molecular Biology Society of Japan
  • Int'l Joint Research / Invited
• [Presentation] Presynaptic MAST Kinase Controls Bidirectional Post-Synaptic Responses to Convey Stimulus Valence during C. elegans Thermotaxis (2019)
  • Author(s): Shunji Nakano, Muneki Ikeda, Yuki Tsukada, Xianfeng Fei, Takamasa Suzuki, Rhea Ahluwalia, Ayana Sano, Rumi Kondo, Kunio Ihara, Koichi Hashimoto, Tetsuya Higashiyama & Ikue Mori
  • Organizer: The 22nd International C. elegans meeting
  • Int'l Joint Research
• [Presentation] Alteration of Synaptic Valence Controls a C. elegans Navigation Behavior (2019)
  • Author(s): Shunji Nakano
  • Organizer: Japan-UK neuroscience symposium
• [Presentation] Alteration between positive and negative valence of a neuronal connection shapes a C. elegans navigation behavior (2018)
  • Author(s): Shunji Nakano, Muneki Ikeda, Yuki Tsukada, Ayana Sano, Rumi Kondo, Takamasa Suzuki, Tetsuya Higashiyama, and Ikue Mori
  • Organizer: The 41st annual meeting of the Japan neuroscience society
  • Invited