Publicly Offered Research
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
To determine how developmental clock processes, regulate dendrite arbor patterning, recently we used in vivo live imaging of Drosophila c sensory neurons to follow the complete temporal sequence of arbor patterning events. Key to this is the timing of a switch in growth rates: early establishment of the main arbor field requires rapid outgrowth; this then transitions into a slow growing maturation phase. We predict that the developmental clock which times this transition consists of both a cell intrinsic counting component and anexternal regulatory component.
The nervous system expands massively in size from its formation during embryogenesis until maturity; insufficient brain growth is a significant cause of intellectual disability. As neurons don’t divide, this expansion is supported primarily through enormous cellular growth that scales proportionally with the growth of the organism. Here, we utilized Drosophila larval body wall sensory neurons as a model system to investigate this scaling program. We carried out genome-wide mapping of transcription start sites at single base pair-resolution during scaling. Through motif- and Chip seq- activity mapping, we identified the prevalent chromatin architectural proteins that drive these promoter cohorts to power cellular scaling. In parallel, through systematic genetic screening of the loci controlled by these promoter cohorts, coupled with multi-dimensional morphometric analysis, we identified key cell biological effectors of this program. We found that these effectors of scaling also introduce variance to dendritic parameters of individual neurons, allowing for diversification of arbor morphology;a trait argued to potentially contribute to the emergence of higher order nervous system activity. Some neuron types are bigger than others; for example, the large class IV da neurons grow quicker and to a larger mature arbor size than other body wall sensory neurons.
令和2年度が最終年度であるため、記入しない。
All 2020 2019
All Journal Article (1 results) (of which Int'l Joint Research: 1 results, Peer Reviewed: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results, Invited: 3 results)
Neuron
Volume: online Issue: 3 Pages: 452-467.e8
10.1016/j.neuron.2020.02.002
