Role of DNA topology in gene expression.
Publicly Offered Research
Project Area | Chromatin potential for gene regulation |
Project/Area Number |
19H05261
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Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | Kyoto University |
Principal Investigator |
Canela Andres 京都大学, 白眉センター, 特定准教授 (90837585)
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Project Period (FY) |
2019-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2020: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | DNA Topoisomerases / Transcription / DNA torsion / RNA polymerase II / Chromatin loop / DNA torsions / RNA polymerase 2 |
Outline of Research at the Start |
How torsional tension in the chromatin impacts transcription? which steps are affected? To this end, I will set up a model to measure changes in the rates of RNA polymerase binding, pause release and transcriptional burst frequency with a combination of genomics and single-cell imaging.
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Outline of Annual Research Achievements |
During the fiscal year 2020, I evaluated the impact of torsional stress in transcription using cell lines deficient for DNA topoisomerase 2 (TOP2α and TOP2β) generated in the fiscal year 2019. Combining nascent RNA FISH with ChIP-seq for PolII I quantified how the torsional stress accumulated in absence of topoisomerase 2 activity affects transcription dynamics. I measured transcription bursting, intensity of transcription and rates of PollII binding and pause release
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Research Progress Status |
令和2年度が最終年度であるため、記入しない。
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Strategy for Future Research Activity |
令和2年度が最終年度であるため、記入しない。
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Report
(2 results)
Research Products
(11 results)
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[Journal Article] Neuronal enhancers are hotspots for DNA single-strand break repair.2021
Author(s)
Wu W, Hill SE, Nathan WJ, Paiano J, Callen E, Wang D, Shinoda K, van Wietmarschen N, Colon-Mercado JM, Zong D, De Pace R, Shih HY, Coon S, Parsadanian M, Pavani R, Hanzlikova H, Park S, Jung SK, McHugh PJ, Canela A, Chen C, Casellas R, Caldecott KW, Ward ME, Nussenzweig A.
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Journal Title
Nature
Volume: online
Issue: 7859
Pages: 440-444
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] 53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination.2020
Author(s)
Callen E, Zong D, Wu W, Wong N, Stanlie A, Ishikawa M, Pavani R, Dumitrache LC, Byrum AK, Mendez-Dorantes C, Martinez P, Canela A, Maman Y, Day A, Kruhlak MJ, Blasco MA, Stark JM, Mosammaparast N, McKinnon PJ, Nussenzweig A
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Journal Title
Molecular Cell
Volume: 77
Issue: 1
Pages: 26-38
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Topoisomerase II-Induced Chromosome Breakage and Translocation Is Determined by Chromosome Architecture and Transcriptional Activity.2019
Author(s)
Canela A, Maman Y, Huang SN, Wutz G, Tang W, Zagnoli-Vieira G, Callen E, Wong N, Day A, Peters JM, Caldecott KW, Pommier Y, Nussenzweig A
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Journal Title
Molecular Cell
Volume: 75
Issue: 2
Pages: 252-266
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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