発動分子の化学-力学エネルギー変換機構の解明に資する高速AFM技術の開発

• Project Area: Molecular Engine: Design of Autonomous Functions through Energy Conversion
• Project/Area Number: 19H05389
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Nagoya University
• Principal Investigator: 内橋 貴之 名古屋大学, 理学研究科, 教授 (30326300)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 高速原子間力顕微鏡 / 発動分子 / 動態計測 / モータータンパク質 / 一分子計測 / タンパク質 / 構造変化 / 蛍光顕微鏡

Outline of Research at the Start

本領域で創成された発動分子やその集積体の構造動態や動的相互作用、秩序形成のダイナミクス観察に関して共同研究を推進する。同時に、計測技術・シミュレーション・理論班との協働により発動分子の作動原理を実験・理論両面から明らかにする。一方で、回転分子モーターV-ATPaseに焦点を絞り、高速AFM/一分子蛍光顕微鏡を用いてATPの結合と解離の検出と同時に分子の構造変化を測定する方法を確立し、化学-力学エネルギー変換の分子機構を明らかにする。また、高速AFM/一分子蛍光顕微鏡装置に実装する多機能基板の開発も進め、外部刺激による発動分子の動態応答に関する研究を推進する。

Outline of Annual Research Achievements

i) フェリチンケージ分解過程の直接観察(A01-3上野Gとの領域内共同研究)：鉄を貯蔵タンパク質フェリチンは自己集合によってかご状構造体を形成することが知られており, ナノ材料を調製するためのテンプレートとして広く使用されてきた。このかご状構造体は溶液のpHに依存して分解及び再構成されることが知られているが, それらの開始や中間状態に関与するダイナミクスは解明されていなかった。高速AFMで, 溶液中の単一のフェリチンケージのpH依存的な分解過程を観察した結果, かご状構造体が断片に分解する前に穴が形成されることを明らかにした。MDシミュレーションの結果, 穴はフェリチンタンパク質３個で構成される３回対称チャネルの開口形成でトリガーされることを明らかにした。（Basudev et al, PCCP 2020）
ii) 微小管の変形によるキネシンの運動速度変化( B01　角五Gとの領域内共研究)：マイカ基板にアイランド上に平面脂質膜を形成し、さらに脂質膜を構成する正電荷脂質の量を制御することで、微小管を屈曲して基板に固定することができた。この微小管上で微小管関連運動タンパク質であるキネシンの滑走運動を高速AFMで観察した結果、微小管の変形が細胞内輸送に関与する滑走速度を制御していることが明らかになった。屈曲の曲率が異なる微小管でのキネシンの移動速度を解析したところ、曲率が大きくなるほどキネシンの運動速度が低下した。分子動力学シミュレーションでキネシンの運動速度低下の要因を探ったところ、変形した微小管ではキネシンの親和性が高まっているためであることがわかった。この結果は、キネシンの移動を制御するためのメカノセンサーとしての微小管の役割を明らかにし、微小管の機械的変形がキネシンの移動を制御する役割を果たしていることを示唆している。

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Non-close-packed arrangement of soft elastomer microspheres on solid substrates (2021)
  • Author(s): Yuma Sasaki, Seina Hiroshige, Masaya Takizawa, Yuichiro Nishizawa, Takayuki Uchihashi, Haruka Minato and Daisuke Suzuki
  • Journal Title: RSC Advances Volume: 11 Issue: 24 Pages: 14562-14567
  • DOI: 10.1039/d1ra02688g
  • Peer Reviewed / Open Access
• [Journal Article] Nanostructure and thermoresponsiveness of poly(N-isopropyl methacrylamide)-based hydrogel microspheres prepared via aqueous free radical precipitation polymerization (2021)
  • Author(s): Nishizawa Yuichiro、Minato Haruka、Inui Takumi、Saito Ikuma、Kureha Takuma、Shibayama Mitsuhiro、Uchihashi Takayuki、Suzuki Daisuke
  • Journal Title: RSC Advances Volume: 11 Issue: 22 Pages: 13130-13137
  • DOI: 10.1039/d1ra01650d
  • Peer Reviewed / Open Access
• [Journal Article] Dynamic Assembly/Disassembly of Staphylococcus aureus FtsZ Visualized by High-Speed Atomic Force Microscopy (2021)
  • Author(s): Fujita Junso、Sugiyama Shogo、Terakado Haruna、Miyazaki Maho、Ozawa Mayuki、Ueda Nanami、Kuroda Natsuko、Tanaka Shun-ichi、Yoshizawa Takuya、Uchihashi Takayuki、Matsumura Hiroyoshi
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 4 Pages: 1697-1697
  • DOI: 10.3390/ijms22041697
  • Peer Reviewed / Open Access
• [Journal Article] Single-molecule level dynamic observation of disassembly of the apo-ferritin cage in solution (2020)
  • Author(s): Maity Basudev、Li Zhipeng、Niwase Kento、Ganser Christian、Furuta Tadaomi、Uchihashi Takayuki、Lu Diannan、Ueno Takafumi
  • Journal Title: Physical Chemistry Chemical Physics Volume: 22 Issue: 33 Pages: 18562-18572
  • DOI: 10.1039/d0cp02069a
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Nanostructures, Thermoresponsiveness, and Assembly Mechanism of Hydrogel Microspheres during Aqueous Free-Radical Precipitation Polymerization (2020)
  • Author(s): Nishizawa Yuichiro、Minato Haruka、Inui Takumi、Uchihashi Takayuki、Suzuki Daisuke
  • Journal Title: Langmuir Volume: 37 Issue: 1 Pages: 151-159
  • DOI: 10.1021/acs.langmuir.0c02654
  • Peer Reviewed
• [Journal Article] Novel Babesia bovis exported proteins that modify properties of infected red blood cells (2020)
  • Author(s): Hakimi H, Templeton TJ, Sakaguchi M, Yamagishi J, Miyazaki S, Yahata K, Uchihashi T, Kawazu SI, Kaneko O, Asada M
  • Journal Title: PLoS Pathogens Volume: 16(10) Issue: 10 Pages: e1008917-e1008917
  • DOI: 10.1371/journal.ppat.1008917
  • NAID: 120006988490
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural insights into the mechanism of rhodopsin phosphodiesterase (2020)
  • Author(s): Ikuta T, Shihoya W, Sugiura M, Yoshida K, Watari M, Tokano T, Yamashita K, Katayama K, Tsunoda SP, Uchihashi T, Kandori H and Nureki O.
  • Journal Title: Nat. Commun. Volume: 11 Issue: 1 Pages: 5605-5605
  • DOI: 10.1038/s41467-020-19376-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Convergent evolution of processivity in bacterial and fungal cellulases (2020)
  • Author(s): Uchiyama, T., Uchihashi, T., Nakamura, A., Watanabe, H., Kaneko, S., Samejima, M., and Igarashi K.
  • Journal Title: Proc. Nat. Acad. Sci. U.S.A. Volume: 117 Issue: 33 Pages: 19896-19903
  • DOI: 10.1073/pnas.2011366117
  • Peer Reviewed / Open Access
• [Journal Article] Direct visualization of the conformational change of FUS/TLS upon binding to promoter-associated non-coding RNA (2020)
  • Author(s): Nesreen Hamad, Hiroki Watanabe, Takayuki Uchihashi, Riki Kurokawa, Takashi Nagata, and Masato Katahira
  • Journal Title: Chemical Communications Volume: 56 Issue: 64 Pages: 9134-9137
  • DOI: 10.1039/d0cc03776a
  • Peer Reviewed / Open Access
• [Journal Article] Structural Dynamics of a Protein Domain Relevant to the Water-Oxidizing Complex in Photosystem II as Visualized by High-Speed Atomic Force Microscopy (2020)
  • Author(s): Tokano Takaya、Kato Yuki、Sugiyama Shogo、Uchihashi Takayuki、Noguchi Takumi
  • Journal Title: The Journal of Physical Chemistry B Volume: 124 Issue: 28 Pages: 5847-5857
  • DOI: 10.1021/acs.jpcb.0c03892
  • Peer Reviewed
• [Journal Article] Assembly mechanism of a supramolecular MS-ring complex to initiate bacterial flagellar biogenesis in Vibrio species. (2020)
  • Author(s): Terashima H, Hirano K, Inoue Y, Tokano T, Kawamoto A, Kato T, Yamaguchi E, Namba K, Uchihashi T, Kojima S, Homma M.
  • Journal Title: Journal of Bacteriology Volume: - Issue: 16
  • DOI: 10.1128/jb.00236-20
  • Peer Reviewed
• [Journal Article] Thermoresponsive structural changes of single poly(N-isopropyl acrylamide) hydrogel microspheres under densely packed conditions on a solid substrate (2020)
  • Author(s): Minato Haruka、Nishizawa Yuichiro、Uchihashi Takayuki、Suzuki Daisuke
  • Journal Title: Polymer Journal Volume: 52 Issue: 9 Pages: 1137-1141
  • DOI: 10.1038/s41428-020-0372-3
  • NAID: 40022335719
  • Peer Reviewed
• [Journal Article] Dynamic behavior of an artificial protein needle contacting a membrane observed by high-speed atomic force microscopy (2020)
  • Author(s): Ueno Takafumi、Niwase Kento、Tsubokawa Daisho、Kikuchi Kosuke、Takai Natsumi、Furuta Tadaomi、Kawano Ryuji、Uchihashi Takayuki
  • Journal Title: Nanoscale Volume: in press Issue: 15 Pages: 8166-8173
  • DOI: 10.1039/d0nr01121e
  • Peer Reviewed
• [Journal Article] Construction of a Hexameric Hemoprotein Sheet and Direct Observation of Dynamic Processes of Its Formation (2020)
  • Author(s): Oohora Koji、Hirayama Shota、Uchihashi Takayuki、Hayashi Takashi
  • Journal Title: Chemistry Letters Volume: 49 Issue: 2 Pages: 186-190
  • DOI: 10.1246/cl.190855
  • NAID: 130007795242
  • Peer Reviewed
• [Journal Article] Thermoresponsive Micellar Assembly Constructed from a Hexameric Hemoprotein Modified with Poly(N-isopropylacrylamide) toward an Artificial Light-Harvesting System (2020)
  • Author(s): Hirayama Shota、Oohora Koji、Uchihashi Takayuki、Hayashi Takashi
  • Journal Title: Journal of the American Chemical Society Volume: 142 Issue: 4 Pages: 1822-1831
  • DOI: 10.1021/jacs.9b10080
  • Peer Reviewed / Open Access
• [Journal Article] Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge (2020)
  • Author(s): Tatebe Hisashi、Lim Chew Theng、Konno Hiroki、Shiozaki Kazuhiro、Shinohara Akira、Uchihashi Takayuki、Furukohri Asako
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 370-370
  • DOI: 10.1038/s41467-019-14025-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Supramolecular tholos-like architecture constituted by archaeal proteins without functional annotation. (2020)
  • Author(s): Maho Yagi-Utsumi,Arunima Sikdar,Chihong Song,Jimin Park,Rintaro Inoue,Hiroki Watanabe,Raymond N Burton-Smith,Toshiya Kozai,Tatsuya Suzuki,Atsuji Kodama,Kentaro Ishii,Hirokazu Yagi,Tadashi Satoh,Susumu Uchiyama,Takayuki Uchihashi,Keehyoung Joo,Jooyoung Lee,Masaaki Sugiyama,Kazuyoshi Murata,Koichi Kato
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 1540-1540
  • DOI: 10.1038/s41598-020-58371-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Single-molecule imaging analysis reveals the mechanism of a high-catalytic-activity mutant of chitinase A from Serratia marcescens (2020)
  • Author(s): Visootsat A, Nakamura A, Vignon P, Watanabe H, Uchihashi T, Iino R
  • Journal Title: J Biol Chem Volume: 295 Issue: 7 Pages: 1915-1925
  • DOI: 10.1074/jbc.ra119.012078
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hydrogel Microellipsoids that Form Robust String-like Assemblies at the Air/Water Interface (2019)
  • Author(s): Honda Kenshiro、Sazuka Yuka、Iizuka Kojiro、Matsui Shusuke、Uchihashi Takayuki、Kureha Takuma、Shibayama Mitsuhiro、Watanabe Takumi、Suzuki Daisuke
  • Journal Title: Angewandte Chemie International Edition Volume: 印刷中 Issue: 22 Pages: 7294-7298
  • DOI: 10.1002/anie.201901611
  • Peer Reviewed
• [Journal Article] Construction of a quadrangular tetramer and a cage-like hexamer from three-helix bundle-linked fusion proteins (2019)
  • Author(s): Miyamoto Takaaki、Hayashi Yugo、Yoshida Keito、Watanabe Hiroki、Uchihashi Takayuki、Yonezawa Kento、Shimizu Nobutaka、Kamikubo Hironari、Hirota Shun
  • Journal Title: ACS Synthetic Biology Volume: 印刷中 Issue: 5 Pages: 1112-1120
  • DOI: 10.1021/acssynbio.9b00019
  • NAID: 120006801961
  • Peer Reviewed / Open Access
• [Journal Article] Non‐Thermoresponsive Decanano‐sized Domains in Thermoresponsive Hydrogel Microspheres Revealed by Temperature‐Controlled High‐Speed Atomic Force Microscopy (2019)
  • Author(s): Nishizawa Yuichiro、Matsui Shusuke、Urayama Kenji、Kureha Takuma、Shibayama Mitsuhiro、Uchihashi Takayuki、Suzuki Daisuke
  • Journal Title: Angewandte Chemie International Edition Volume: 58 Issue: 26 Pages: 8809-8813
  • DOI: 10.1002/anie.201903483
  • Peer Reviewed
• [Journal Article] Mutational and Combinatorial Control of Self-Assembling and Disassembling of Human Proteasome α Subunits (2019)
  • Author(s): Taichiro Sekiguchi, Tadashi Satoh, Eiji Kurimoto, Chihong Son, Toshiya Kozai, Hiroki Watanabe, Kentaro Ishii, Hirokazu Yagi, Saeko Yanaka Susumu Uchiyama, Takayuki Uchihashi, Kazuyoshi Murata, Koichi Kato
  • Journal Title: International Journal of Molecular Sciences Volume: 20 Pages: 2608-2608
  • Peer Reviewed / Open Access
• [Journal Article] High-speed AFM reveals accelerated binding of agitoxin-2 to a K+ channel by induced fit (2019)
  • Author(s): A. Sumino,T. Sumikama,T. Uchihashi,S. Oiki
  • Journal Title: Science Advances Volume: 5 Issue: 7 Pages: 1-10
  • DOI: 10.1126/sciadv.aax0495
  • NAID: 120006649788
  • Peer Reviewed / Open Access
• [Journal Article] Dynamics of Inter-Molecular Interactions Between Single Aβ42 Oligomeric and Aggregate Species by High-Speed Atomic Force Microscopy (2019)
  • Author(s): Lei Feng, Hiroki Wantanabe, Paul Molino, Gordon Wallace, Son Lam Phung, Takayuki Uchihashi, Michael Higgins
  • Journal Title: Journal of Molecular Biology Volume: 431 Issue: 15 Pages: 2687-2699
  • DOI: 10.1016/j.jmb.2019.04.044
  • Int'l Joint Research
• [Journal Article] Protein uptake into individual hydrogel microspheres visualized by high-speed atomic force microscopy. (2019)
  • Author(s): Shusuke Matsui,Kensuke Hosho,Haruka Minato,Takayuki Uchihashi,Daisuke Suzuki
  • Journal Title: Chemical Communications Volume: 55 Issue: 68 Pages: 10064-10067
  • DOI: 10.1039/c9cc05116c
  • Peer Reviewed
• [Journal Article] Crystal structure of heliorhodopsin (2019)
  • Author(s): W. Shihoya, K. Inoue, M. Singh, M. Konno, S. Hososhima, K. Yamashita, K. Ikeda, A. Higuchi, T. Izume, Okazaki S, Hashimoto M, Mizutori R, Tomida S, Yamauchi Y, Abe-Yoshizumi R, K. Katayama, SP. Tsunoda, M. Shibata, Y. Furutani, A. Pushkarev, O. Béjà, T. Uchihashi, H. Kandori, O. Nureki
  • Journal Title: Nature Volume: 574 Issue: 7776 Pages: 132-136
  • DOI: 10.1038/s41586-019-1604-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III. (2019)
  • Author(s): [Rina Yogo,Yuki Yamaguchi,Hiroki Watanabe,Hirokazu Yagi,Tadashi Satoh,Mahito Nakanishi,Masayoshi Onitsuka,Takeshi Omasa,Mari Shimada,Takahiro Maruno,Tetsuo Torisu,Shio Watanabe,Daisuke Higo,Takayuki Uchihashi,Saeko Yanaka,Susumu Uchiyama,Koichi Kato]
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 11957-11957
  • DOI: 10.1038/s41598-019-48323-w
  • NAID: 120006875664
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] On-Membrane Dynamic Interplay between Anti-GM1 IgG Antibodies and Complement Component C1q. (2019)
  • Author(s): Saeko Yanaka,Rina Yogo,Hiroki Watanabe,Yuki Taniguchi,Tadashi Satoh,Naoko Komura,Hiromune Ando,Hirokazu Yagi,Nobuhiro Yuki,Takayuki Uchihashi,Koichi Kato
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Issue: 1 Pages: 147-147
  • DOI: 10.3390/ijms21010147
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 高速AFMによる酸素発生光化学系II のドメイン構造揺らぎの可視化 (2020)
  • Author(s): 内橋貴之
  • Organizer: ブルカージャパン オンラインシンポジウム ～単一生体分子の動的プロセス評価に向けた高速AFM技術～
  • Invited
• [Presentation] 高速原子間力顕微鏡でタンパク質の一分子動態を可視化する (2020)
  • Author(s): 内橋貴之
  • Organizer: 新学術領域合同シンポジウム－ソフトロボット学と発動分子科学の境界
  • Invited
• [Presentation] 高速原子間力顕微鏡で可視化する生体・人工分子のダイナミクス (2020)
  • Author(s): 内橋貴之
  • Organizer: 岡山大学大学院医歯薬学総合研究科(薬学系) 先端薬学特論 公開セミナー
  • Invited
• [Presentation] HS-AFM as a versatile tool to study dynamical and mechanical properties of proteins (2020)
  • Author(s): Christian Ganser, Kimitoshi Takeda, Ryota Iino, Koichi Kato and Takayuki Uchihashi
  • Organizer: 第58回日本生物物理学会年会: Symposium "The Multiple Modes of Proteins ? From Molecular Dynamics to Pathogenesis -
  • Int'l Joint Research / Invited
• [Presentation] 高速原子間顕微鏡による膜輸送装置Secの動態観察 (2020)
  • Author(s): 長池航, 板家成良, 春山隆充, 塚崎智也, 内橋貴之
  • Organizer: 日本生体エネルギー研究会第46回討論会
• [Presentation] 高速原子間力顕微鏡を用いた光化学系IIの動態観察 (2020)
  • Author(s): 戸叶貴也, 加藤祐樹, 杉山翔吾, 野口巧, 内橋貴之
  • Organizer: 日本生体エネルギー研究会第46回討論会
• [Presentation] Observation of Substrate Binding Sec Translocon and Structural Change of SecA with HS-AFM (2020)
  • Author(s): Wataru Nagaike, Takamitsu Haruyama, Tomoya Tsukazaki and Takayuki Uchihashi
  • Organizer: 第58回日本生物物理学会年会
  • Int'l Joint Research
• [Presentation] 溶液中HS-AFM observation of Amyloid β elongation and inhibition by antibodies (2020)
  • Author(s): Shogo Miyajima, Maho Yagi-Utsumi, Takayuki Uchihashi and Koichi Kato
  • Organizer: 第58回日本生物物理学会年会
  • Int'l Joint Research
• [Presentation] Chasing the transformation between monomer and dimer structure of cadherin anchored to supported lipid bilayer by high-speed AFM (2020)
  • Author(s): Shigetaka Nishiguchi, Hiroki Oda and Takayuki Uchihashi
  • Organizer: 第58回日本生物物理学会年会
  • Int'l Joint Research
• [Presentation] Real-Time Visualization of Biological Molecules at Work with High-Speed Atomic Force Microscopy (2019)
  • Author(s): Takayuki Uchihashi
  • Organizer: IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale 2019
  • Int'l Joint Research / Invited
• [Presentation] The 2nd NIBB-Princeton Joint Symposium on Imaging and Quantitative Biology (2019)
  • Author(s): Takayuki Uchihashi
  • Organizer: Imaging of Single-Molecule Dynamics using High-Speed Atomic Force Microscopy
  • Int'l Joint Research / Invited
• [Presentation] Real-Time Monitoring of Morphological Change of Metal Surface During Corrosion Process with High-Speed Atomic Force Microscopy (2019)
  • Author(s): Takayuki Uchihashi, Mitsuhiro Okada, Miyuki Tanaka, Toshitaka Kubo, Tetsuo Shimizu
  • Organizer: 27th International Colloquium on Scanning Probe Microscopy (ICSPM27)
  • Int'l Joint Research
• [Presentation] 高速原子間力顕微鏡と生体・人工分子のダイナミクス計測 (2019)
  • Author(s): 内橋貴之
  • Organizer: 第23回コロイド・界面技術者フォーラム～コロイド領域のイメージング・評価技術～
  • Invited
• [Presentation] ダイナミンの動的分子イメージング：in vitro 再構成系×高速 AFM (2019)
  • Author(s): 竹居孝二, 内橋貴之
  • Organizer: ワークショップ「新しいコラボの在り方を探る～細胞生物学ｘ蛋白質科学」 第19回 日本蛋白質科学会年会, 第71回 日本細胞生物学会大会 合同年次大会
• [Presentation] 溶液中高速原子間力顕微鏡による生体・人工分子のダイナミクス解析 (2019)
  • Author(s): 内橋貴之
  • Organizer: シンポジウム「原子間力顕微鏡技術のパラダイムシフト～原子から細胞まで階層をまたぐ構造・機能解析～」 日本顕微鏡学会 第75回学術講演会
• [Presentation] 高速原子間力顕微鏡による生細胞イメージング (2019)
  • Author(s): 内橋貴之
  • Organizer: 日本学術振興会 ナノプローブテクノロジー第167委員会 第91回研究会
  • Invited
• [Presentation] 高速原子間力顕微鏡で可視化する分子ダイナミクス (2019)
  • Author(s): 内橋貴之
  • Organizer: 日本学術振興会 情報科学用有機材料第142委員会 合同研究会
  • Invited
• [Book] 膜タンパク質工学ハンドブック:第1編 第2章15「高速原子間力顕微鏡によるタンパク質の構造ダイナミクス解析」 (2020)
  • Author(s): 内橋貴之
  • Total Pages: 624
  • Publisher: 株式会社 エヌ・ティー・エス
  • ISBN: 9784860435370
• [Book] 自己修復材料、 自己組織化、形状記憶材料の開発と応用事例 (2020)
  • Author(s): 内橋 貴之
  • Total Pages: 446
  • Publisher: （株）技術情報協会
  • ISBN: 9784861047817
• [Remarks] 名古屋大学D研ホームページ
  • URL: http://d.phys.nagoya-u.ac.jp/
• [Remarks] 名古屋大学D研ホームページ
  • URL: http://d.phys.nagoya-u.ac.jp/index.html