原子レベルで制御されたモデル有機材料の化学構造-水和構造-界面現象の包括的研究

• Project Area: Aquatic Functional Materials: Creation of New Materials Science for Environment-Friendly and Active Functions
• Project/Area Number: 20H05210
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: 林 智広 東京工業大学, 物質理工学院, 准教授 (30401574)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2021: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ナノバイオテクノロジー / 表面・界面科学 / 界面科学 / 表面科学

Outline of Research at the Start

本研究では材料の化学構造、それによって決定する局所水和構造、水和によって引き起こされる界面相互作用を系統的に解析する。具体的には精密表面間力測定(原子間力顕微鏡:AFM)で観測されるナノメートルスケールで作用する相互作用と、AFMで可視化される水和構造、表面増強赤外分光・X線発光分光測定で明らかとなる界面水分子の水素結合状態の関係を詳細に解析する。

Outline of Annual Research Achievements

本研究課題においては、モデル有機物表面である金ーチオール系の自己組織化単分子膜(
Self-assembled monolayers: SAMs)の近傍の水分子の振る舞いの解析のため原子間力顕微鏡(Atomic force microscopy: AFM)、X線発光分光法(X-ray photoemission spectroscopy)を用いた解析を行った。その結果、抗付着性(抗タンパク質吸着・抗細胞接着特性)を持つ単分子膜近傍には、バルクの水とは異なり、タンパク質あるいは細胞の接近を防ぐ様な界面の水分子の障壁があることを報告した。またこの水分子層の障壁は、抗付着性SAMの親水性・疎水性に関わらず存在することが明らかとなり、界面の水分子の振る舞いと抗付着性の関係性が明らかとなった。
また、本課題においては情報科学的手法(機械学習・多変量解析)を用いてSAMを構成する分子の化学構造から水への濡れ性、タンパク質(フィブリノーゲン)の吸着量を予測するアルゴリズムの構築を行った。この成果は情報科学を用いたバイオマテリアル設計の先駆的成果として、新聞、WEBメディアなどでも紹介された。

Research Progress Status

令和3年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和3年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Design of Biointerfaces Using Informatics : From Monolayers to Polymer Surfaces (2022)
  • Author(s): Debabrata Palai, 千頭 俊太, 前田 翔一, 田原 寛之, 林 智広
  • Journal Title: Vacuum and Surface Science Volume: 65 Issue: 1 Pages: 10-14
  • DOI: 10.1380/vss.65.10
  • NAID: 130008140711
  • ISSN: 2433-5835, 2433-5843
  • Year and Date: 2022-01-10
  • Open Access
• [Journal Article] Interphase Protein Layers Formed on Self-Assembled Monolayers in Crowded Biological Environments: Analysis by Surface Force and Quartz Crystal Microbalance Measurements (2022)
  • Author(s): Evan Angelo Quimada Mondarte, Elisa Margarita Mendoza Zamarripa, Ryongsok Chang, Fan Wang, Subin Song, Hiroyuki Tahara, Tomohiro Hayashi
  • Journal Title: Langmuir Volume: 38 Issue: 4 Pages: 1324-1333
  • DOI: 10.1021/acs.langmuir.1c02312
  • Peer Reviewed / Open Access
• [Journal Article] Formation and superlattice of long-range and highly ordered alicyclic selenolate monolayers on Au(1 1 1) studied by scanning tunneling microscopy (2022)
  • Author(s): Hungu Kang, Hyerim Jeong, Sicheon Seong, Seulki Han, Young Ji Son, Hiroyuki Tahara, Tomohiro Hayashi, Hyo Jae Yoon, Jaegeun Noh
  • Journal Title: Applied Surface Science Volume: 572 Pages: 151454-151454
  • DOI: 10.1016/j.apsusc.2021.151454
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 表面・界面科学の手法による生体分子・細胞と人工材料の界面の分子プロセスの解析 (2022)
  • Author(s): 林 智広
  • Journal Title: バイオマテリアル－生体材料－ Volume: 40 Pages: 38-38
• [Journal Article] Analysis of Vicinal Water in Soft Contact Lenses Using a Combination of Infrared Absorption Spectroscopy and Multivariate Curve Resolution (2022)
  • Author(s): Shoichi Maeda, Shunta Chikami, Glenn Villena Latag, Subin Song, Norio Iwakiri, Tomohiro Hayashi
  • Journal Title: Molecules Volume: 27 Issue: 7 Pages: 2130-2130
  • DOI: 10.3390/molecules27072130
  • Peer Reviewed / Open Access
• [Journal Article] Lab on a Tip: Atomic Force Microscopy as a Versatile Analytical Tool for Nano-bioscience (2021)
  • Author(s): Evan Angelo Quimada Mondarte, Hiroyuki Tahara, Kasinan Suthiwanich, Subin Song, Fan Wang,Tomohiro Hayashi
  • Journal Title: Sensors and Materials Volume: 33 Issue: 1 Pages: 223
  • DOI: 10.18494/SAM.2021.3069
  • ISSN: 0914-4935, 2435-0869
  • Year and Date: 2021-01-22
  • Peer Reviewed / Open Access
• [Journal Article] Water at interfaces: its behavior and roles in interfacial phenomena (2021)
  • Author(s): Tomohiro Hayashi
  • Journal Title: Chemistry Letters Volume: - Issue: 6 Pages: 1173-1180
  • DOI: 10.1246/cl.210049
  • NAID: 130008053415
  • Peer Reviewed / Open Access
• [Journal Article] Dendronized oligoethylene glycols with phosphonate tweezers for cell-repellent coating of oxide surfaces: coarse-scale and nanoscopic interfacial forces (2021)
  • Author(s): Julian Czajor, Wasim Abuillan, Dinh Vu Nguyen, Christopher Heidebrecht, Evan Angelo Mondarte, Oleg V Konovalov, Tomohiro Hayashi, Delphine Felder-Flesch, Stefan Kaufmann, Motomu Tanaka
  • Journal Title: RSC Advances Volume: 11 Issue: 29 Pages: 17727-17733
  • DOI: 10.1039/d1ra02571f
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] AIを用いたバイオマテリアルの設計 (2021)
  • Author(s): 林 智広
  • Journal Title: 工業材料 (日刊工業新聞社) Volume: 69 Pages: 59-63
• [Journal Article] Chemical force spectroscopic mapping of nanoparticle-embedded polyester fiber by atomic force microscopy (2021)
  • Author(s): Taichi Nakamura, Evan Angelo Quimada Mondarte, Hiroyuki Tahara, Ryongsok Chang, Tomohiro Hayashi
  • Journal Title: Molecular Crystals and Liquid Crystals Volume: 728 Issue: 1 Pages: 52-58
  • DOI: 10.1080/15421406.2021.1946977
  • Peer Reviewed / Open Access
• [Journal Article] Protein- and Cell-Resistance of Zwitterionic Peptide-Based Self-Assembled Monolayers: Anti-Biofouling Tests and Surface Force Analysis (2021)
  • Author(s): Ryongsok Chang, Evan Angelo Quimada Mondarte, Debabrata Palai, Taito Sekine, Aki Kashiwazaki, Daiki Murakami, Masaru Tanaka, Tomohiro Hayashi
  • Journal Title: Front Chem. Volume: 9 Pages: 748017-748017
  • DOI: 10.3389/fchem.2021.748017
  • Peer Reviewed / Open Access
• [Journal Article] Tyrosine-based photoluminescent diketopiperazine supramolecular aggregates (2021)
  • Author(s): Noritaka Shimosaraya, Taichi Sotani, Yu Miyagi, Evan Angelo Quimada Mondarte, Kasinan Suthiwanich, Tomohiro Hayashi, Yuuya Nagata, Hiromitsu Sogawa, Fumio Sanda
  • Journal Title: Soft Matter Volume: 18 Issue: 1 Pages: 137-145
  • DOI: 10.1039/d1sm01206a
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of Polyacrylonitrile Nonwoven Mats and Silver?Gold Bimetallic Nanoparticle?Decorated Nonwoven Mats for Potential Promotion of Wound Healing In Vitro and In Vivo and Bone Growth In Vitro (2021)
  • Author(s): Meng-Yi Bai, Fang-Yu Ku, Jia-Fwu Shyu, Tomohiro Hayashi, Chia-Chun Wu
  • Journal Title: Polymers (Basel) Volume: 13(4) Issue: 4 Pages: 516-516
  • DOI: 10.3390/polym13040516
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Visualization of molecular binding sites at the nanoscale in the lift-up mode by amplitude-modulation atomic force microscopy (2021)
  • Author(s): Maekawa Tatsuhiro, Nyu Takashi, Mondarte Evan Angelo Quimada, Tahara Hiroyuki, Suthiwanich Kasinan, Hayashi Tomohiro
  • Journal Title: Nanoscale Volume: 7 Issue: 7 Pages: 4213-4220
  • DOI: 10.1039/d0nr06125e
  • Peer Reviewed / Open Access
• [Journal Article] Analysis of specific interactions of biomolecules by atomic force microscopy (2020)
  • Author(s): キマダ モンダルテ エヴァン アンジェロ, 田原 寛之, 張 嶺碩, 林 智広
  • Journal Title: Oyo Buturi Volume: 89 Issue: 7 Pages: 390-393
  • DOI: 10.11470/oubutsu.89.7_390
  • NAID: 130007871667
  • ISSN: 0369-8009, 2188-2290
  • Year and Date: 2020-07-10
  • Open Access
• [Journal Article] Anti-biofouling Surfaces Made of Biomolecules (2020)
  • Author(s): 張嶺碩, E. A. Mondarte, E. Mendoza, 田原寛之,林智広
  • Journal Title: Vacuum and Surface Science Volume: 63 Issue: 6 Pages: 289-293
  • DOI: 10.1380/vss.63.289
  • NAID: 130007853643
  • ISSN: 2433-5835, 2433-5843
  • Year and Date: 2020-06-10
  • Peer Reviewed / Open Access
• [Journal Article] Scanning tunneling microscopy study on phase behavior of self-assembled monolayers formed by coadsorption of octanethiol and octyl thiocyanate on Au(111) (2020)
  • Author(s): Youngsik Choi, Sicheon Seong, Seulki Han, Young Ji Son, Evan Angelo Quimada Mondarte, Hiroyuki Tahara, Subin Song, Tomohiro Hayashi, Jaegeun Noh
  • Journal Title: Thin Solid Films Volume: 707 Pages: 138100-138100
  • DOI: 10.1016/j.tsf.2020.138100
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Formation and Surface Structure of Highly Ordered Self-Assembled Monolayers of Alkyl Selenocyanates on Au(111) via Ambient-Pressure Vapor Deposition (2020)
  • Author(s): Seulki Han, Sicheon Seong, Young Ji Son, Yasuyuki Yokota, Tomohiro Hayashi, Masahiko Hara, Jaegeun Noh
  • Journal Title: J. Phys. Chem. C Volume: 124 Issue: 49 Pages: 26730-26740
  • DOI: 10.1021/acs.jpcc.0c07401
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Living Ring-Expansion Polymerization of Thiirane with Cyclic Monocarbamothioates (2020)
  • Author(s): Kudo Hiroto, Naritomi Kazunori, Onishi Shuto, Maekawa Hiroyuki, Mondarte Evan Angelo Quimada, Suthiwanich Kasinan, Hayashi Tomohiro
  • Journal Title: Macromolecules Volume: 53 Issue: 12 Pages: 4733-4740
  • DOI: 10.1021/acs.macromol.0c00687
  • Peer Reviewed / Open Access
• [Journal Article] Data-Driven Prediction of Protein Adsorption on Self-Assembled Monolayers toward Material Screening and Design (2020)
  • Author(s): Rudolf Jason Kwaria, Evan Angelo Quimada Mondarte, Hiroyuki Tahara, Ryongsok Chang, tomohiro Hayash
  • Journal Title: ACS Biomaterials Science & Engineering Volume: 6(9) Issue: 9 Pages: 4949-4956
  • DOI: 10.1021/acsbiomaterials.0c01008
  • Peer Reviewed / Open Access
• [Journal Article] Selective electro-oxidation of glycerol to dihydroxyacetone by a non-precious electrocatalyst ? CuO (2020)
  • Author(s): CinLiu, Makoto Hirohara, Tatsuhiro Maekawa, Ryongsok Chang, Tomohiro Hayashi, Cia-Ying Chiang
  • Journal Title: Applied Catalysis B: Environmental Volume: 265 Pages: 118543-118543
  • DOI: 10.1016/j.apcatb.2019.118543
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Study on Bacterial Anti-adhesiveness of Stiffness and Thickness Tunable Cross-linked Phospholipid Copolymer Thin-film (2020)
  • Author(s): Zhou Lu, Evan Angelo Mondarte, Kasinan Suthiwanich, Tomohiro Hayashi, Tsukuru Masuda, Norifumi Isu, Madoka Takai
  • Journal Title: ACS Applied Bio Materials Volume: 3(2) Issue: 2 Pages: 1079-1079
  • DOI: 10.1021/acsabm.9b01041
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Imaging nanophase-separated structure of block copolymer by atomic force microscopy in Aqueous Solution (2020)
  • Author(s): Kasinan Suthiwanich, Yukari Hiraguchi, Takashi Nyu, Evan Angelo Quimada Mondarte, Madoka Takai,Tomohiro Hayashi
  • Journal Title: Chemistry Letters Volume: 49 Issue: 6 Pages: 641-644
  • DOI: 10.1246/cl.190894
  • NAID: 130007852062
  • Peer Reviewed / Open Access
• [Journal Article] Real-time and in situ observation of structural evolution of giant block copolymer thin film under solvent vapor annealing by atomic force microscopy (2020)
  • Author(s): Takano Kaori、Nyu Takashi、Maekawa Tatsuhiro、Seki Takashi、Nakatani Ryuichi、Komamura Takahiro、Hayakawa Teruaki、Hayashi Tomohiro
  • Journal Title: RSC Advances Volume: 10 Issue: 1 Pages: 70-75
  • DOI: 10.1039/c9ra09043f
  • Peer Reviewed / Open Access
• [Journal Article] 材料科学と情報科学の出会い: バイオマテリアル分野の現状 (2020)
  • Author(s): 林智広
  • Journal Title: 研究開発リーダー Volume: 16（10） Pages: 22-25
• [Journal Article] 林智広. データ科学で医療材料を創る (2020)
  • Author(s): 林智広
  • Journal Title: みらいぶっく～学問・大学なび学校法人河合塾 Volume: -
• [Journal Article] 原子間力顕微鏡を用いたバイオ界面の解析: 水中での評価技術の発展と代表的成果 (2020)
  • Author(s): 林智広
  • Journal Title: 水中・液中における測定・評価と応用技術 Volume: -
• [Journal Article] Molecular Dynamics Study on the Water Mobility and Side-Chain Flexibility of Hydrated Poly(ω-methoxyalkyl acrylate)s (2020)
  • Author(s): An-Tsung Kuo, Shingo Urata, Ryohei Koguchi, Toshiki Sonoda, Shingo Kobayashi, Masaru Tanaka
  • Journal Title: ACS Biomaterials Science & Engineering Volume: 6 Pages: 6690-6700
  • DOI: 10.1016/j.colsurfb.2020.111449
  • Peer Reviewed / Open Access
• [Presentation] マテリアルズインフォマティクスを利用した抗付着性表面の設計 (2022)
  • Author(s): 林 智広
  • Organizer: 新化学技術推進協会（JACI） 材料分科会
  • Invited
• [Presentation] Analysis of molecular processes at biointerfaces and data-driven biomaterials design (2022)
  • Author(s): 林 智広
  • Organizer: NIMS ICYS ( (International Center for Young Scientists) Workshop
  • Int'l Joint Research / Invited
• [Presentation] Mechanism underlying anti-biofouling: behavior of molecules at bio interfaces (2022)
  • Author(s): 林 智広
  • Organizer: Aquaphotomics Spring Seminar
  • Int'l Joint Research / Invited
• [Presentation] 表面科学の手法を用いたバイオマテリアル近傍の水の解析 (2022)
  • Author(s): 林 智広
  • Organizer: 電子情報通信学会 有機エレクトロニクス研究会（OME）
• [Presentation] A new interface-sensitive analytical method based on a combination of linear spectroscopy and informatics (2022)
  • Author(s): Subin Song, Tomohiro Hayashi
  • Organizer: 第69回応用物理学会春季学術講演会
• [Presentation] Evaluation of viscoelasticity of water layers on model solid surfaces by quartz crystal microbalance with energy dissipation monitoring (QCM-D) (2022)
  • Author(s): Subin Song, Evan Angelo Quimada Mondarte, Tomohiro Hayashi
  • Organizer: 第69回応用物理学会春季学術講演会
• [Presentation] Investigation of Bacterial Adhesion on Various Surfaces using Quartz Crystal Microbalance with Energy Dissipation (QCM-D) Monitoring (2022)
  • Author(s): Glenn Villena Latag, Taichi Nakamura, Evan Angelo Quimada Mondarte, Tomohiro Hayashi
  • Organizer: 第69回応用物理学会春季学術講演会
• [Presentation] ソフトコンタクトレンズの界面水の水素結合状態の解析 (2022)
  • Author(s): 前田翔一, 千頭俊太, Subin Song, Glenn Latag, 林智広
  • Organizer: 第69回応用物理学会春季学術講演会
• [Presentation] 固体表面に形成された細胞認識サイトのナノスケール分解能での可視化 (2022)
  • Author(s): 田原寛之, 張嶺碩, Evan Angelo Quimada Mondarte, 林智広
  • Organizer: 第69回応用物理学会春季学術講演会
• [Presentation] 実験と機械学習によるバイオマテリアルの評価・設計: 実験科学者が使う情報科学 (2021)
  • Author(s): 林 智広
  • Organizer: 電気学会
  • Invited
• [Presentation] コンビナトリアル実験と情報科学の融合による生体材料の設計 (2021)
  • Author(s): 林 智広
  • Organizer: 日本ゴム協会年次大会
  • Invited
• [Presentation] バイオマテリアルの生体適合性のメカニズム解明およびデータ科学による材料設計 (2021)
  • Author(s): 林 智広
  • Organizer: S&Tセミナー
  • Invited
• [Presentation] Anti-biofouling Monolayers: The Mechanism and Molecular Designs (2021)
  • Author(s): 林 智広
  • Organizer: MRS Workshop Series on Innovations in Biomaterials Science: Machine Learning and Artificial Intelligence in Biomaterials Design and Development
  • Int'l Joint Research / Invited
• [Presentation] バイオ界面解析とインフォマティクスとの融合 (2021)
  • Author(s): 林 智広
  • Organizer: 日本化学会 CSJ 化学フェスタ
  • Invited
• [Presentation] バイオマテリアルの表面構造と界面相互作用 (2021)
  • Author(s): 林 智広
  • Organizer: 物質・デバイス領域共同研究拠点 アライアンス研究会「バイオ界面水の役割と機能-生物相分離における界面科学」
  • Invited
• [Presentation] Design of novel RNA hydrogel materials made of self-assembling RNA higher-order structures (2021)
  • Author(s): Josephine Galipon, Karim Raqbi, Lala Ito, Arisa Tsuruoka, Masaru Tomita, Ryongsok Chang, Mondarte Evan Angelo Quimada, Tomohiro Hayashi, H. Furukawa
  • Organizer: 第22回日本RNA学会年会
• [Presentation] Interactions at nanoscales at biointerfaces (2021)
  • Author(s): Subin Song, Fan Wang, Enkhriimaa Bat-Erdene, Tomohiro Hayashi
  • Organizer: KJF-ICOMEP 2021
  • Int'l Joint Research
• [Presentation] 抗付着性単分子膜が引き起こすナノメートルスケールの相互作用 (2021)
  • Author(s): 林智広
  • Organizer: 第70回高分子討論会
• [Presentation] 固体表面上に吸着したタンパク質が形成する細胞認識サイトのナノスケール分解能での可視化 (2021)
  • Author(s): 田原寛之, 張嶺碩, Evan Angelo Quimada Mondarte, 林智広
  • Organizer: 第82回応用物理学会秋季学術講演会
• [Presentation] Data-driven prediction of protein adsorption on model organic surfaces: from self-assembled monolayers to polymer surfaces (2021)
  • Author(s): Debabrata Palai, Shunta Chikami, Shoichi Maeda, Tomohiro Hayashi
  • Organizer: MRM 2021 Yokohama
  • Int'l Joint Research
• [Presentation] Water at biointerfaces: what makes surfaces bioinert? (2021)
  • Author(s): 林智広
  • Organizer: The 4th Aquaphotomics International Conference
• [Presentation] Data-driven prediction of protein adsorption on self-assembled monolayers:toward material screening and design (2020)
  • Author(s): 林智広
  • Organizer: 第30回日本MRS-J年次大会
• [Presentation] バイオマテリアルと生体分子・細胞の相互作用の理解および材料情報学による設計最適化 (2020)
  • Author(s): 林智広
  • Organizer: S&Tセミナー
• [Presentation] 情報科学を利用したソフトバイオマテリアルの開発 (2020)
  • Author(s): 林智広
  • Organizer: 日本ゴム協会年次大会
• [Presentation] Probing the macromolecular crowding condition at the interfacial level using colloidal probe atomic force microscopy (2020)
  • Author(s): Evan Angelo Quimada Mondarte, Elisa Margarita Mendoza Zamarripa, Ryongsok Chang,Tomohiro Hayashi
  • Organizer: 第30回MRS-J年次大会
• [Presentation] バクテリア接着を規制する高分子薄膜物性 (2020)
  • Author(s): 高井まどか, 林智広, 陸洲, 増田造, Evan Angelo Qimada Mondarte, Suthiwanich Kasinan,井須紀文
  • Organizer: 第69回高分子討論会
• [Book] 水中・液中における測定・評価と応用技術 (2020)
  • Author(s): 大西 洋, 森口 志穂, 林 智広, 木之下 博, 松本 直浩, 小椋 俊彦, 大久保 信明, 高山 博光, 紙野 圭, 細田 奈麻絵, 鳥村 政基, 中山 敦好, 国岡 正雄, 本間 寿, 敷野 修, 糸﨑 秀夫, 加藤 亮, 蜂屋 弘之, 今城 勝治, 吉田 弘
  • Total Pages: 165
  • Publisher: S&T出版
  • ISBN: 9784907002824
• [Remarks] 林研究室WEBサイト
• [Remarks] 林研究室WEBサイト
  • URL: http://lab.spm.jp/