Publicly Offered Research
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Recently, using X-ray free electron laser (XFEL) , various dynamic processes associated with proteins have been elucidated. However, all such studies by serial femtosecond crystallography have been limited to only natural proteins and the methodology has not been applied tosynthetic organic molecules or metal complexes. In this work, we plan to establish a method to study process of a chemical reaction by serial crystallography. We will study the CO photo-release reaction from Lys-Mn(CO)3 composite and ring-closing-opening reaction of photochromic diarylethene molecules.
In this research, we demonstrated a visualization of photolysis reaction by a synthetic metal complex using TR-SFX method. We fixed a photoactive CO releasing Mn(CO)3 reaction center into porous Lysozyme microcrystals. X-ray crystal structure revealed an octahedral Mn reaction center with 3CO and two water molecules. Three CO ligands oriented in a facial manner. By controlling the time and light dose, we succeeded in determining the CO release process and structure of a bicarbonyl intermediate species in real-time. In addition, we performed quantum chemical calculations which was in good agreement with the experimental results. The demonstration has a great scope to explore the mechanism of difficult organic, inorganic, or synthetic metalloprotein reactions.
令和3年度が最終年度であるため、記入しない。
All 2021 2020
All Presentation (4 results)
