Site-Selective Protein Chemical Modification of Exposed Tyrosine Residues Using Tyrosine Click Reaction
Publicly Offered Research
| Project Area | Non-equilibrium-state molecular movies and their applications |
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| Project/Area Number |
20H05438
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Complex systems
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Maity Basudev 東京工業大学, 生命理工学院, 特任助教 (60815421)
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | CO release reaction / Initial intermediate / TR-SFX method / Lysozyme / Serial crystallography / Lysozyme-Mn(CO)3 / CO photo-release |
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| Outline of Research at the Start |
Recently, using X-ray free electron laser (XFEL) , various dynamic processes associated with proteins have been elucidated. However, all such studies by serial femtosecond crystallography have been limited to only natural proteins and the methodology has not been applied tosynthetic organic molecules or metal complexes. In this work, we plan to establish a method to study process of a chemical reaction by serial crystallography. We will study the CO photo-release reaction from Lys-Mn(CO)3 composite and ring-closing-opening reaction of photochromic diarylethene molecules.
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| Outline of Annual Research Achievements |
In this research, we demonstrated a visualization of photolysis reaction by a synthetic metal complex using TR-SFX method. We fixed a photoactive CO releasing Mn(CO)3 reaction center into porous Lysozyme microcrystals. X-ray crystal structure revealed an octahedral Mn reaction center with 3CO and two water molecules. Three CO ligands oriented in a facial manner. By controlling the time and light dose, we succeeded in determining the CO release process and structure of a bicarbonyl intermediate species in real-time. In addition, we performed quantum chemical calculations which was in good agreement with the experimental results. The demonstration has a great scope to explore the mechanism of difficult organic, inorganic, or synthetic metalloprotein reactions.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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Report
(2 results)
Research Products
(4 results)
