• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

潜在性結核対策を想定した、抗酸菌の長期生存を可能にする分子メカニズムの解明

Publicly Offered Research

Project AreaMatrix of Infection Phenomena
Project/Area Number 21022043
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOsaka City University

Principal Investigator

松本 壮吉  大阪市立大学, 大学院・医学研究科, 准教授 (30244073)

Co-Investigator(Kenkyū-buntansha) 仁木 満美子 (吉村 満美子)  大阪市立大学, 大学院・医学研究科, 助教 (20438229)
岡 真優子  大阪市立大学, 大学院・医学研究科, 研究員 (40347498)
Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 2010: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2009: ¥3,800,000 (Direct Cost: ¥3,800,000)
Keywords結核 / 抗酸菌 / 休眠 / 潜在感染 / ゲノム
Research Abstract

抗酸菌感染症は現在も甚大な健康被害を及ぼしている。特に結核は年間180万人が死亡する最大級の細菌感染症である。結核菌は、人類の1/3に休眠状態で潜伏感染しており、感染者の10%において再燃が生じ病気が発症する。結核菌はヒト以外の宿主や自然環境下で生息できないため、潜伏感染菌の殺傷は病気の制圧に繋がるが、現在まで休眠機構は不明で潜伏感染菌を殺傷することができない。このような状況から抗酸菌の休眠機構を解明し対策を構築することは急務の課題であり本研究の目的である。
本研究は、休眠期に制御される抗酸菌分子で休眠菌の長期生存を担う分子を同定し、各分子の機能を解析することで、休眠抗酸菌の生存維持のメカニズム解明に迫ることを目的とした。平成21年度には、休眠Mycobacterium smegmatisが発現する遺伝子群の同定を行った。その結果、M.smegmatis遺伝子のうち1200あまりは休眠期で発現が減少するが、逆に900あまりの遺伝子は増加することが判明した。また休眠の導入や維持に重要な役割を果たす蛋白質分子MDP1の欠失で休眠期に減少する遺伝子が約700、逆に増加するものが約500観察された。以上の結果から、M.smegmatis休眠菌は遺伝子の発現を継続していること、またMDP1が休眠期に発現する遺伝子の多くを制御していることが明らかとなった。22年度には、これらの遺伝子発現の変化が、MDP1のゲノムへの直接的結合に依存するのかを明らかにするため、MDP1が結合するゲノム領域をクロマチン沈降法によって沈降させ、その遺伝子配列を次世代シーケンサーで解析する、Chip-sequence法を実施した。現在、次世代シーケンサーによる大規模データを解析しており仮説を検証している。

Report

(2 results)
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (28 results)

All 2011 2010 2009

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (8 results) Book (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Transient role of CD4+CD25+ regulatory T cells in mycobacterial infection in mice.2010

    • Author(s)
      Ozeki, Y., I.Sugawara, T.Udagawa, T.Aoki, M.Osada-Oka, Y.Tateishi, H.Hisaeda, Y.Nishiuchi, N.Harada, K.Kobayashi, S.Matsumoto.
    • Journal Title

      Int Immunol

      Volume: 22 Pages: 179-189

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Controlled expression of branch-forming mannosyltransferase is critical for mycobacterial lipoarabinomannan biosynthesis.2010

    • Author(s)
      Sena, C.B., T.Fukuda, K.Miyanagi, S.Matsumoto, K.Kobayashi, Y.Murakami, Y.Maeda, T.Kinoshita, Y.S.Morita.
    • Journal Title

      J Biol Chem

      Volume: 285 Pages: 13326-13336

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of murine T-cell epitopes on mycobacterial DNA-binding protein 1 (MDP1) using DNA vaccination.2010

    • Author(s)
      Suzuki, D., T.Nagata, G.Eweda, S.Matsumoto, M.Matsumoto, K.Tsujimura, Y.Koide.
    • Journal Title

      Vaccine

      Volume: 28 Pages: 2020-2025

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential recruitment of CD63 and Rab7-interacting- lysosomal-protein to phagosomes containing Mycobacterium tuberculosis in macrophages.2010

    • Author(s)
      Seto, S., S.Matsumoto, K.Tsujimura, Y.Koide.
    • Journal Title

      Microbiol Immunol

      Volume: 54 Pages: 170-174

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of avian influenza virus H5 DNA vaccine and MDP-1 gene of Mycobacterium bovis as genetic adjuvant.2010

    • Author(s)
      Jalilian, B., A.R.Omar, M.H.Bejo, N.B.Alitheen, M.Rasoli, S.Matsumoto.
    • Journal Title

      Genet Vaccines Ther

      Volume: 8 Pages: 4-4

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of murine T-cell epitopes on mycobacterial DNA-binding protein 1 (MDP1) using DNA vaccination.2010

    • Author(s)
      Suzuki, D., S.Matsumoto
    • Journal Title

      Vaccine (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential recruitment of CD63 and Rab7-interacting-lysosomal-protein to phagosomes containing Mycobacterium tuberculosis in macrophages.2010

    • Author(s)
      Seto, S., S.Matsumoto
    • Journal Title

      Microbiol Immunol (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Controlled expression of branch-forming mannosyltransferase is critical for mycobacterial lipoarabinomannan biosynthesis.2010

    • Author(s)
      Sena, C.B., S.Matsumoto
    • Journal Title

      J Biol Chem (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transient role of CD4+CD25+ regulatory T cells in mycobacterial infection in mice.2010

    • Author(s)
      Ozeki, Y., S.Matsuxnoto.
    • Journal Title

      Int Immunol (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mycobacteria Exploit Host Hyaluronan for Efficient Extracellular Replication.2009

    • Author(s)
      Hirayama, Y., S.Matsumoto.
    • Journal Title

      PLoS Pathog 5

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dissection of Rab7 localization on Mycobacterium tuberculosis phagosome.2009

    • Author(s)
      Seto, S., S.Matsumoto, I.Ohta, K.Tsujimura, Y.Koide.
    • Journal Title

      Biochem Biophys Res Commun 387

      Pages: 272-277

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Virulence of Mycobacterium avium complex strains isolated from immunocompetent patients.2009

    • Author(s)
      Tateishi, Y., S.Matsumoto.
    • Journal Title

      Microb Pathog 46

      Pages: 6-12

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High transmissibility of the modern Beijing Mycobacterium tuberculosis in homeless patients of Japan.2009

    • Author(s)
      Wada, T., S.Matsumoto
    • Journal Title

      Tuberculosis (Edinb) 19

      Pages: 19-19

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mycobacterium kyorinense sp.nov., a novel, slow-growing species, related to Mycobacteriuxn celatum, isolated from human clinical specimens.2009

    • Author(s)
      Okazaki, M., S.Matsumoto
    • Journal Title

      Int J Syst Evol Microbiol 59

      Pages: 1336-1341

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mycobacterium avium Complex Organisms Predominantly Colonize in the Bathtub Inlets of Patients' Bathrooms.2009

    • Author(s)
      Nishiuchi, Y., S.Matsumoto
    • Journal Title

      Jpn J Infect Dis 62

      Pages: 182-186

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Growth inhibition of HeLa cell by internalization of Mycobacterium bovis Bacillus Calmette-Guerin (BCG) Tokyo.2009

    • Author(s)
      Kitamura. A., S.Matsumoto, I.Asahina.
    • Journal Title

      Cancer Cell Int 9

      Pages: 30-30

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 結核菌の長生きのメカニズム;休眠現象と潜在性結核2010

    • Author(s)
      松本壮吉
    • Organizer
      第27回東海薬物治療研究会
    • Place of Presentation
      名古屋(AP名古屋)(招待講演)
    • Year and Date
      2010-05-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 抗酸菌感染症における感染制御の進歩2010

    • Author(s)
      松本壮吉
    • Organizer
      第84回日本感染症学会総会
    • Place of Presentation
      京都(国立京都国際会館)(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mycobacteria exploit host hyaluronan for efficient extracellular replication2010

    • Author(s)
      松本壮吉
    • Organizer
      International Society for Hyaluronan Sciences 8th Internarional Congerence Hyaluronan 2010
    • Place of Presentation
      京都(ブライトンホテル)(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 結核菌の気道ヒアルロン酸を利用した感染と生体内増殖2010

    • Author(s)
      松本壮吉
    • Organizer
      第22回微生物シンポジウム
    • Place of Presentation
      大阪(大阪薬科大学)(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] A histone-like protein of mycobacteria possesses ferritin superfamily protein-like activities : ferric iron storage and ferrous iron oxidation2010

    • Author(s)
      Mayuko Osada-Oka, Masaki Takatsuka, Kazuo kobayashi, Sohkichi Matsumoto.
    • Organizer
      The 10th Awaji International Forum on Infection and Immunity
    • Place of Presentation
      淡路島(淡路夢舞台国際会議場)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Clinical Practice for the Diagnosis of Latent Mycobacterium tuberculosis Infection2010

    • Author(s)
      Mayuko Osada-Oka, Yukio Hirayama, Seigo Kitada, Yoshitaka Tateishi, Ryoji Maekura, Haruhiko Sugimura, Yukio Koide, Kazuo Kobayashi, Sohkichi Matsumoto.
    • Organizer
      14th International Conference on Emerging Infectious Diseases (EID) in the Pacific Rim
    • Place of Presentation
      Malaysia
    • Related Report
      2010 Annual Research Report
  • [Presentation] Transient role of CD4^+CD25^+regulatory T cells in Mycobacterium tuberculosis infection in mice.2010

    • Author(s)
      Ozeki, I.Sugawara, T.Udagawa, H.Hisaeda, K.Kobayashi, S. Matsumoto.
    • Organizer
      14^<th> International Congress of Immunology
    • Place of Presentation
      神戸(神戸ポートピアホテル、神戸国際展示場)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 気道ヒアルロン酸を利用した、結核菌の感染と増殖のストラテジー2009

    • Author(s)
      松本壮吉
    • Organizer
      第50回日本熱帯医学学会大会
    • Place of Presentation
      沖縄
    • Related Report
      2009 Annual Research Report
  • [Book] 人獣共通感染症、非結核性抗酸菌症 改訂版2010

    • Author(s)
      西内由紀子、立石善隆、山田毅、松本壮吉
    • Total Pages
      551
    • Publisher
      医薬ジャーナル社
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 免疫刺激G9.1の抗結核ブースターワクチン創出への応用2011

    • Inventor(s)
      伊保澄子, 前山順一, 松本壮吉, 山本三郎
    • Industrial Property Rights Holder
      国立大学法人福井大学, 国立感染症研究所長, 公立大学法人大阪市立大学, 日本ビーシージー製造株式会社
    • Industrial Property Number
      2011-080646
    • Filing Date
      2011-03-31
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] MDP1による微生物を凝集および/または沈殿させる方法2009

    • Inventor(s)
      山本法明、松本壮吉
    • Industrial Property Rights Holder
      コニカミノルタホ-ルディングス株式会社
    • Filing Date
      2009-06-29
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] 遅発育性抗酸菌ポリペプチド2009

    • Inventor(s)
      山田毅、松本壮吉
    • Industrial Property Rights Holder
      大塚製薬株式会社
    • Acquisition Date
      2009-12-04
    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2018-03-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi