色覚オプシンの種内多様性と環境適応

• Project Area: Molecular interaction and modal shift of cellular sensors
• Project/Area Number: 21026007
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: 河村 正二 東京大学, 大学院・新領域創成科学研究科, 教授 (40282727)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥5,800,000 (Direct Cost: ¥5,800,000); Fiscal Year 2010: ¥2,900,000 (Direct Cost: ¥2,900,000); Fiscal Year 2009: ¥2,900,000 (Direct Cost: ¥2,900,000)
• Keywords: 色覚 / オプシン / 視物質 / 種内変異 / 集団遺伝学

Research Abstract

視覚オプシンの遺伝子重複と重複オプシン遺伝子間の吸収波長の分化及び発現パターンの分化は視覚系進化の重要なステップである。しかし、発現パターンの分化のメカニズムの理解は吸収波長の分化のメカニズムに比べて非常に遅れている。ゼブラフィッシュ(Danio rerio)は2つの赤型錐体オプシン遺伝子LWS-1とLWS-2を持つ。これらはゲノム中で順方向に隣接して並んでおり、共に網膜の複錐体長形細胞(long member of double cones(LDCs))に特異的に発現している。網膜の中央から背側と後方にかけてLWS-2が発現し、網膜の周縁、特に腹側と前方の広い領域でLWS-1が発現するという発現の空間分化が生じている。そこで我々はこれら2つの遺伝子の発現分化に関わるシス制御因子の同定を試みた。LWS-1とLWS-2を含むゼブラフィッシュのゲノム領域約100kbのP1-artificial chromosome(PAC)クローンを公共遺伝子バンクから取得し、これらのPACクローンとGFP等の蛍光レポーター遺伝子を活用してゼブラフィッシュ生体への遺伝子導入レポーターアッセイを行なった。その結果、両方の遺伝子の発現制御に関わる約0.6kbの領域をLWS-1上流に見出し、"LWS-activating region"(LAR)と命名した。LARはLWS-1とLWS-2の双方の発現を増強させるエンハンサーとして機能しており、2つの遺伝子のプロモーターとLARとの相互作用は2つの遺伝子間で競合的であり、その競合のパターンは確率的ではなく発生段階でプログラムされていると考えられた。

Research Products

• [Journal Article] Polymorphic color vision in primates : evolutionary considerations (2011)
  • Author(s): Kawamura, S., Hiramatsu, C., Melin, A.D., Schaffner, C.M, Aureli, F., Fedigan, L.M.
  • Journal Title: Post Genome Biology of Primates (Hirai, H., Imai, H.and Go, Y.eds.), Springer, Tokyo, Japan(書籍の1章) Volume: (掲載確定)
  • Peer Reviewed
• [Journal Article] Evolutionary diversification of visual opsin genes in fish and primates (2011)
  • Author(s): Kawamura, S.
  • Journal Title: From Genes to Animal Behavior : Social Structures, Personalities, Communication by Color (Inoue-Murayama, M., Kawamura, S.and Weiss, A.eds.), Springer, Tokyo, Japan(書籍の1章) Pages: 329-349
  • Peer Reviewed
• [Journal Article] A single enhancer regulating the differential expression of duplicated red-sensitive opsin genes in zebrafish (2010)
  • Author(s): Tsujimura, T., Hosoya, T., Kawamura, S.
  • Journal Title: PLoS Genetics Volume: 6
  • Peer Reviewed
• [Journal Article] Interplay of olfaction and vision in fruit foraging of spider monkeys (2009)
  • Author(s): Hiramatsu, C., Melin, A.D., Aureli, F., Schaffner, C.M., Vorobyev, M., Kawamura, S.
  • Journal Title: Animal Behaviour 77 Pages: 1421-1426
  • Peer Reviewed
• [Journal Article] Medaka : a promising model animal for comparative population genomics (2009)
  • Author(s): Matsumoto, Y., Oota, H., Asaoka, Y., Nishina, H., Watanabe, K., Bujnicki, J.M., Oda, S., Kawamura, S., Mitani, H.
  • Journal Title: BMC Research Notes 2
  • Peer Reviewed
• [Journal Article] Genetic differentiation among local populations of medaka fish(Oryzias latipes)evaluated through grid- and deme-based sampling (2009)
  • Author(s): Katsumura, T., Oda, S., Mano, S., Suguro, N., Watanabe, K., Mitani, H., Oota, H, Kawamura, S.
  • Journal Title: Gene 443 Pages: 170-177
  • Peer Reviewed
• [Journal Article] A common variation in EDAR is a genetic determinant of shovel-shaped incisors (2009)
  • Author(s): Kimura, R., Yamaguchi, T., Takeda, M., Kondo, O., Toma, T., Haneji, K., Hanihara, T., Matsukusa, H., Kawamura, S., Maki, K., Osawa, M., Ishida, H., Onta, H.
  • Journal Title: The American Journal of Human Genetics 85 Pages: 528-535
  • Peer Reviewed
• [Journal Article] Fig foraging by dichromatic and trichromatic white-faced capuchin monkeys in a tropical dry forest (2009)
  • Author(s): Melin, A.D., Fedigan, L.M., Hiramatsu, C., Hiwatashi, T., Parr, N., Kawamura, S.
  • Journal Title: International Journal of Primatology 30 Pages: 753-775
  • Peer Reviewed
• [Journal Article] An explicit signature of balancing selection for color-vision variation in New World monkeys (2009)
  • Author(s): Hiwatashi, T., Okabe, Y., Tautsui, T., Hiramatsu, C., Melin, A.D., Oota, H., Schaffner, C.M., Aureli, F., Fedigan, L.M., Innan, H., Kawamura, S.
  • Journal Title: Molecular Biology and Evolution 27 Pages: 453-464
  • Peer Reviewed
• [Journal Article] Population-specific susceptibility to Crohn's disease and ulcerative colitis ; dominant and recessive relative risks in the Japanese population (2009)
  • Author(s): Nakagome, S., Takeyama, Y., Mano, S., Sakisaka, S., Matsui, T., Kawamura, S., Oota, H.
  • Journal Title: Annals of Human Genetics 74 Pages: 126-136
  • Peer Reviewed
• [Journal Article] (訳者ノート)霊長類の色覚:3色型が進化した理由は? (2009)
  • Author(s): 河村正二
  • Journal Title: 日経サイエンス 39 Pages: 72-73
• [Journal Article] 錐体オプシン遺伝子と色覚の進化多様性:魚類と霊長類に注目して (2009)
  • Author(s): 河村正二
  • Journal Title: 比較生理生化学 26 Pages: 110-116
  • NAID: 10025617644
  • Peer Reviewed
• [Journal Article] 色覚の進化 (2009)
  • Author(s): 河村正二
  • Journal Title: 東京大学大学院新領域創成科学研究科広報誌創成 14 Pages: 5-5
• [Presentation] ヒトL/Mオプシン遺伝子の多様性と色覚変異の起源及び中立性の検討 (2010)
  • Author(s): 河村正二
  • Organizer: 戦略的研究プロジェクト研究「現生人類の拡散による遺伝子と文化の多様性創出に関する総合的研究」第1回全体会議
  • Place of Presentation: 総合研究大学院大学、葉山
  • Year and Date: 2010-12-03
• [Presentation] 新世界ザルホエザル属におけるヒト型3色型色覚仮説の検証に向けて (2010)
  • Author(s): 松下裕香、太田博樹、Welker, B、河村正二
  • Organizer: 第64回日本人類学会
  • Place of Presentation: だて歴史の杜カルチャーセンター、北海道伊達市
  • Year and Date: 2010-10-01
• [Presentation] Toward deeper understanding color vision of howler monkeys (2010)
  • Author(s): Matsushita, Y., Oota, H., Welker, B., Kawamura, S.
  • Organizer: The 23rd International Primatological Society Congress Kyoto 2010
  • Place of Presentation: Kyoto University (Yoshida Main Campus), Kyoto, Japan
  • Year and Date: 2010-09-12
• [Presentation] Challenging advantage of trichromacy in fruit foraging behavior of wild spider monkeys (2010)
  • Author(s): Hiramatsu, C., Melin, A.D., Aureli, F., Schaffner, C.M., Vorobyev, M., Kawamura, S.
  • Organizer: The 23rd International Primatological Society Congress Kyoto 2010
  • Place of Presentation: Kyoto University (Yoshida Main Campus), Kyoto, Japan
  • Year and Date: 2010-09-12
• [Presentation] Dietary selectivity by white-faced capuchins : how important are colourful fruits? (2010)
  • Author(s): Melin, A.D., Fedigan, L.M., Parr, N., Kawamura, S.
  • Organizer: The 23rd International Primatological Society Congress Kyoto 2010
  • Place of Presentation: Kyoto University (Yoshida Main Campus), Kyoto, Japan
  • Year and Date: 2010-09-12
• [Presentation] A population genetic test of balancing selection for color vision variation in New World monkeys (2010)
  • Author(s): Kawamura, S., Hiwatashi, T., Okabe, Y., Tsutsui, T., Hiramatsu, C., Melin, A.D., Oota, H., Schaffner, C.M., Aureli, F., Fedigan, L.M., Innan, H.
  • Organizer: The 23rd International Primatological Society Congress Kyoto 2010
  • Place of Presentation: Kyoto University (Yoshida Main Campus), Kyoto, Japan
  • Year and Date: 2010-09-12
• [Presentation] Identification of a single enhancer regulating differential expression of duplicated red-sensitive opsin genes in zebrafish (2010)
  • Author(s): Tsujimura, T., Ashino, R., Hosoya, T., Kawamura, S.
  • Organizer: The 4th International Symposium of the Biodiversity and Evolution, Kyoto University Global COE project "Evolution of Sensor, Communication and Society"
  • Place of Presentation: Shiran Kaikan, Kyoto University, Kyoto, Japan
  • Year and Date: 2010-09-11
• [Presentation] Molecular genetic reevaluation of the uniform-trichromacy hypothesis for howler monkeys (2010)
  • Author(s): Matsushita, Y., Oota, H., Welker, B., Kawamura, S.
  • Organizer: The 4th International Symposium of the Biodiversity and Evolution, Kyoto University Global COE project "Evolution of Sensor, Communication and Society"
  • Place of Presentation: Shiran Kaikan, Kyoto University, Kyoto, Japan
  • Year and Date: 2010-09-11
• [Presentation] Color vision polymorphism in wild New World monkeys as a model system to understand the adaptive significance of primate color vision (2010)
  • Author(s): Kawamura, S.
  • Organizer: The 4th International Symposium of the Biodiversity and Evolution, Kyoto University Global COE project "Evolution of Sensor, Communication and Society"
  • Place of Presentation: Shiran Kaikan, Kyoto University, Kyoto, Japan
  • Year and Date: 2010-09-11
• [Presentation] グッピーLWSオプシン遺伝子にかかる選択圧の検出 (2010)
  • Author(s): 手塚あゆみ、笠木聡、河村正二、van Oosterhout, C.、松島野枝、河田雅圭
  • Organizer: 第12回日本進化学会
  • Place of Presentation: 東京工業大学 大岡山キャンパス、東京
  • Year and Date: 2010-08-02
• [Presentation] Testing adaptive significance of primate color vision by behavioral and genetic studies of wild populations of New World monkeys (2010)
  • Author(s): Kawamura, S.
  • Organizer: International Symposium on Biodiversity Sciences (ISBDS) 2010 "Genome, Evolution and Environment"
  • Place of Presentation: Nagoya City University (Hotel Rubra Ohzan), Nagoya, Japan
  • Year and Date: 2010-07-31
• [Presentation] Evolution of cis- and trans-acting factors in the regulation of duplicated opsin genes in zebrafish (2010)
  • Author(s): Tsujimura, T., Masuda, R., Matsumoto, Y., Hosoya, T., Oda, S., Mitani, H., Kawamura, S.
  • Organizer: Annual Meeting of the Society of Molecular Biology and Evolution (SMBE) 2010
  • Place of Presentation: Lyon Convention Center, Lyon, France
  • Year and Date: 2010-07-04
• [Presentation] Invertebrate Foraging by Costa Rican Capuchin Monkeys : Testing Predicted Sex Differences in Relation to Color Vision Variation (2010)
  • Author(s): Melin, A.D., Fedigan, L.M., Young, H., Kawamura, S.
  • Organizer: The 33rd Annual Meeting of the American Society of Primatologists
  • Place of Presentation: Seelbach Hilton Hotel, Louisville, Kentucky, USA
  • Year and Date: 2010-06-16
• [Presentation] 新世界ザル野生集団に対する行動観察と遺伝子調査による色覚多型の適応的意義の考察 (2010)
  • Author(s): 河村正二、樋渡智秀、平松千尋、太田博樹、印南秀樹、Melin, A.D.、Fedigam, L.M.、Schaffner, C.M.、Aureli, F.
  • Organizer: 第57回日本生態学会
  • Place of Presentation: 東京大学駒場キャンパス、東京
  • Year and Date: 2010-03-15
• [Presentation] グッピーLWS遺伝子から探る色覚の進化 (2010)
  • Author(s): 手塚あゆみ、笠木聡、河村正二、van Oosterhout, C.、松島野枝、河田雅圭
  • Organizer: 第57回日本生態学会
  • Place of Presentation: 東京大学駒場キャンパス、東京
  • Year and Date: 2010-03-15
• [Presentation] Polymorphic color vision in primates : evolutionary considerations (2010)
  • Author(s): Kawamura, S.
  • Organizer: Hominization Conference 2010 Primate Genomics for Globalization of Collaboration Program : Post-genome biology of primates as the hub-research for understanding our species and neighbors
  • Place of Presentation: Inuyama International Sightseeing Center "Freude", Inuyama, Japan
  • Year and Date: 2010-03-04
• [Presentation] 霊長類における色覚進化の適応的意義を考える (2009)
  • Author(s): 平松千尋、河村正二
  • Organizer: 第41回種生物学シンポジウム「生き物の眼をとおして覗く世界:生理学が支える認知生態学の可能性」
  • Place of Presentation: 八王子セミナーハウス、八王子
  • Year and Date: 2009-12-13
• [Presentation] シトクロムP450遺伝子族のヒト多型と野生メダカ地域集団を用いた機能解析 (2009)
  • Author(s): 勝村啓史、尾田正二、三谷啓志、河村正二、太田博樹
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2009-12-09
• [Presentation] Color vision of non-human primates (2009)
  • Author(s): Mikami, A., Onishi, A., Hanazawa, A., Terao, K., Saito, A., Widayati, K.A., Suryobroto, B., Hiwatashi, T., Kawamura, S., Goto, S., Siriaroonrat, B., Malaivijitnond, S.
  • Organizer: The 3rd International Congress on the Future of Animal Research
  • Place of Presentation: Rose Garden Riverside, Bangkok, Thailand
  • Year and Date: 2009-11-19
• [Presentation] 見ることの進化～ヒトからサカナへ (2009)
  • Author(s): 河村正二
  • Organizer: 東京大学柏キャンパス一般公開柏図書館主催講演会
  • Place of Presentation: 東京大学柏キャンパス柏図書館、柏
  • Year and Date: 2009-10-30
• [Presentation] Medaka(Oryzias latipes)as a model to study human polymorphic functions (2009)
  • Author(s): Katsumura, T., Oda, S., Mitani, H., Kawamura, S., Oota, H.
  • Organizer: The 59th Annual Meeting of The American Society of Human Genetics
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, USA
  • Year and Date: 2009-10-20
• [Presentation] 種内多様度と種間相違度をL-Mオプシン遺伝子と中立対照遺伝子の間で比較することで再評価した新世界ザル自然集団における色覚多型維持に働く平衡選択 (2009)
  • Author(s): 河村正二、樋渡智秀、岡部友吾、筒井登子、平松千尋、太田博樹、印南秀轍
  • Organizer: 第63回日本人類学会
  • Place of Presentation: シェーンバッハ・サボー、東京
  • Year and Date: 2009-10-03
• [Presentation] テナガザルにおけるL-Mオプシン遺伝子座位間 gene conversion と浄化淘汰により形成されるエクソンとイントロンの対照的な多型性パターン (2009)
  • Author(s): 河村正二、樋渡智秀、Suryobroto, B.、Perwitasari-Farajallah, D.、Malaivijitnond, S.、Siriaroonrat, B.、太田博樹、後藤俊二、三上章允
  • Organizer: 第81回日本遺伝学会
  • Place of Presentation: 信州大学理学部、松本
  • Year and Date: 2009-09-16
• [Presentation] コスタリカ・サンタロサ国立公園に生息する野生オマキザル群の遺伝的多様性の解明に向けて (2009)
  • Author(s): 佐藤亮子、Campos, F.、太田博樹、Jack, K.、Fedigan, L.、河村正二
  • Organizer: 第81回日本遺伝学会
  • Place of Presentation: 信州大学理学部、松本
  • Year and Date: 2009-09-16
• [Presentation] グッピーのLWS遺伝子を用いた色覚の多様性の維持機構の解明 (2009)
  • Author(s): 手塚あゆみ、笠木聡、河村正二、van Oosterhout Cock、松島野枝、河田雅圭
  • Organizer: 第11回日本進化学会
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2009-09-02
• [Presentation] メダカを用いたヒト遺伝的多型の生体機能解析実験系の開発 (2009)
  • Author(s): 勝村啓史、尾田正二、三谷啓志、河村正二、太田博樹
  • Organizer: 第11回日本進化学会
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2009-09-02
• [Presentation] Retinal histology and molecular basis color vision in 0-2 age-class Champsocephalus gunnari(Channichthyidae) (2009)
  • Author(s): Miyazaki, T., Iwami, T., Yamauchi, M., Yamauchi, S., Shimizu, R., Kasagi, S., Nakata, M., Kawamura, S.
  • Organizer: The Xth SCAR International Biology Symposium
  • Place of Presentation: Conference hall, Hokkaido University, Sapporo, Japan
  • Year and Date: 2009-07-26
• [Presentation] 新世界ザル自然集団の色覚多型に対する平衡選択の明瞭な証拠 (2009)
  • Author(s): 河村正二、樋渡智秀、岡部友吾、筒井登子、平松千尋、太田博樹、印南秀樹
  • Organizer: 第25回日本霊長類学会
  • Place of Presentation: 中部学院大学各務原キャンパス、各務原
  • Year and Date: 2009-07-18
• [Presentation] 新世界ザル果実採食における視覚および嗅覚の役割 (2009)
  • Author(s): 平松千尋、Melin, A.D.、樋渡智秀、Aureli, F., Schaffner, C.M.、Fedigan, L.M.、Vorobyev, M.、河村正二
  • Organizer: 第25回日本霊長類学会
  • Place of Presentation: 中部学院大学各務原キャンパス、各務原
  • Year and Date: 2009-07-18
• [Presentation] テナガザルL-Mオプシン遺伝子の属を越えた非多型性 (2009)
  • Author(s): 樋渡智秀、白井祐介、三上章允、後藤俊二、Suryobroto, B.、Perwitasari-Farajallah, D.、Malaivijitnond, S.、Sirieroonrat, B.、太田博樹、河村正二
  • Organizer: 第25回日本霊長類学会
  • Place of Presentation: 中部学院大学各務原キャンパス、各務原
  • Year and Date: 2009-07-18
• [Presentation] コスタリカ・サンタロサ国立公園に生息するシロガオオマキザル自然群間の遺伝的関係の解明に向けて (2009)
  • Author(s): 佐藤亮子、Campos, F.、太田博樹、Jack, K.、Fedigan, L.、河村正二
  • Organizer: 第25回日本霊長類学会
  • Place of Presentation: 中部学院大学各務原キャンパス、各務原
  • Year and Date: 2009-07-18
• [Presentation] A population study on CYP genes in medaka(Oryzias latipes)as a model for investigating their functional polymorphisms in humans (2009)
  • Author(s): Katsumura, T., Oda, S., Mitani, H., Kawamura, S., Oota, H.
  • Organizer: 16th International Conference on Cytochrome P450 : Biochemistry, Biophysics, Biotechnology
  • Place of Presentation: Bankoku Shinryokan, Okinawa, Japan
  • Year and Date: 2009-06-21
• [Presentation] 視覚光センサーと色覚の進化多様性:魚類と霊長類から (2009)
  • Author(s): 河村正二
  • Organizer: 東北大学・生命科学研究科・生態適応セミナー
  • Place of Presentation: 東北大学、仙台
  • Year and Date: 2009-04-27
• [Remarks]
  • URL: http://www.jinrui.ib.k.u-tokyo.ac.jp/kawamura-home.html
• [Remarks]
  • URL: http://www.jinrui.ib.k.u-tokyo.ac.jp/kawamura-home.html