ROS生成酵素の網羅的解析に基づく植物の活性酸素シグナルネットワークの解明
Publicly Offered Research
| Project Area | Signaling functions of reactive oxygen species |
|---|
| Project/Area Number |
21117516
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
|
| Allocation Type | Single-year Grants |
|---|
| Review Section |
Biological Sciences
|
|---|
| Research Institution | Tokyo University of Science |
|---|
Principal Investigator |
朽津 和幸 東京理科大学, 理工学部, 教授 (50211884)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
|
|---|
| Keywords | 活性酸素種 / シロイヌナズナ / NADPH oxidase / カルシウムイオン / タンパク質リン酸化 / 花粉 / Rboh / Rac / 異種発現系 / 低分子量Gタンパク質 / 活性酸素種生成酵素 |
|---|
| Research Abstract |
1.植物ROS生成酵素rbohの網羅的解析
シロイヌナズナrboh遺伝子の発現部位の解析と、異種発現系を用いたROS生成活性の解析を並行して進め、両者を総合して、植物体での各遺伝子の機能分担について考察した。
AtrbohH,Jは、花粉に特異的に局在することが明らかになったので、二重突然変異体等を用いて、rbohを介したROSの局所的生成が花粉管の発芽、先端成長の制御に関与している可能性を検証した。
2.rbohの活性制御因子の単離と機能解析
2-1:異種共発現系を用いた活性制御候補因子Racによるrbohの制御機構の解明
シロイヌナズナの11種全てのRac遺伝子をクローニングし、Atrboh遺伝子との異種発現系における共発現解析により、ROS生成量を調べた。
2-2:酵母two-hybrid法を用いた植物rboh新奇活性制御候補因子のスクリーニング
rbohの活性制御にはCa^<2+>の結合とリン酸化以外にも、特にN末端細胞質領域において複数の制御因子との相互作用が関与する可能性がある。そこで、感染防御応答や気孔閉鎖等に関与するAtrbohD,AtrbohFのN末端領域と相互作用する因子を酵母two-hybrid法により単離を試みた.これまでに,相互作用候補因子としてプロテインキナーゼ,防御応答関連因子を含む19種を単離した異種共発現解析系を用いて、rbohのROS生成活性に対する候補因子の共発現の影響について検証した。
|
Report
(2 results)
Research Products
(60 results)
-
[Journal Article] Cryptogein-induced cell cycle arrest at G2 phase is associated with inhibition of cyclin-dependent kinases, suppression of expression of cell cycle-related genes and protein degradation in synchronized tobacco BY-2 cells.2011
Author(s)
Ohno, R., Kadota, Y., Fujii, S., Sekine, M., Umeda, M., Kuchitsu, K.
-
Journal Title
Plant and Cell Physiology
Volume: 52(印刷中)
Related Report
Peer Reviewed
-
-
[Journal Article] Negative Feedback Regulation of Microbe-Associated Molecular Pattern-Induced Cytosolic Ca^<2+> Transients by Protein Phosphorylation.2011
Author(s)
Kurusu, T., Hamada, H., Sugiyama, Y., Yagala, T., Kadota, Y., Furuichi, T., Hayashi, T., Umemura, K., Komatsu, S., Miyao, A., Hirochika, H., Kuchitsu, K.
-
Journal Title
Journal of Plant Research
Volume: 124
Pages: 415-424
NAID
Related Report
Peer Reviewed
-
-
[Journal Article] Regulation of microbe-associated molecular pattern-induced hypersensitive cell death, phytoalexin production and defense gene expression by calcineurin B-like protein-interacting protein kinases, OsCIPK14/15, in rice cultured cells.2010
Author(s)
Kurusu, T., Hamada, J., Nokajima, H., Kitagawa, Y., Kiyoduka, M., Takahashi, A., Hanamata, S., Ohno, R., Hayashi, T., Okada, K., Koga, J., Hirochika, H., Yamane, H., Kuchitsu, K.
-
Journal Title
Plant Physiology
Volume: 153
Pages: 678-692
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Enhanced resistance against both pathogens and insects by overexpression of a tobacco MAP kinase, WIPK.2010
Author(s)
Amano, Y., Seo, S., Kawazu, K., Sato, Y., Sugeno, W., Mochizuki, A., Kuchitsu, K., Ohashi, Y., Mitsuhara, I.
Organizer
国際シンポジウム『微細藻類のバイオテクノロジー～食料・環境・エネルギー』
Place of Presentation
東京大学弥生講堂
Year and Date
2010-05-31
Related Report
-
-
-
-
-
-
-
[Presentation] REGULATION OF MICROBE-ASSOCIATED MOLECULAR PATTERN-INDUCED DEFENSE RESPONSES BY CALCINEURINB-LIKE PROTEIN-INTERACTING PROTEIN KINASES.2010
Author(s)
Kurusu, T., Hamada, J., Nokajima, H., Kitagawa, Y., Kiyoduka, M., Takahashi, A., Hanamata, S., Okada, K., Hirochika, H., Yamane, H., Kuchitsu, K.
Organizer
21st International Conference on Arabidopsis Research
Place of Presentation
パシフィコ横浜
Related Report
-
[Presentation] INTERRELATIONSHIP BETWEEN MAMP-INDUCED DEFENSE RESPONSES AND CELL CYCLE PROGRESSION USING SYNCHRONOUS-CULTURED ARABIDOPSIS CELLS.2010
Author(s)
Takahashi, S., Ohno, R., Maeda, Y., Kimura, S., Abe, Y., Umeda, M., Kuchitsu, K.
Organizer
21st International Conference on Arabidopsis Research
Place of Presentation
パシフィコ横浜
Related Report
-
[Presentation] SCREENING FOR NOVEL REGULATORS OF ATRBOHD AND ATRBOHF, NADAPH OXIDASES INVOLVED IN PRODUCTION OF REACTIVE OXYGEN SPECIES.2010
Author(s)
Kawarazaki, T., Michikawa, M., Imai, A., Niibori, H., Kaya, H., Kuchitsu, K.
Organizer
21st International Conference on Arabidopsis Research
Place of Presentation
パシフィコ横浜
Related Report
-
[Presentation] REGULATION OF ROS-PRODUCING ACTIVITY OF AN ARABIDOPSIS NADPH OXIDASE, ATRBOHF, BY BINDING OF CA^<2+> AND PHOSPHORYLATION.2010
Author(s)
Kimura, S., Okai, M., Kaya, H., Tanokura, M., Kuchitsu, K.
Organizer
21st International Conference on Arabidopsis Research
Place of Presentation
パシフィコ横浜
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
